##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779805_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189987
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.683952059877782	34.0	31.0	34.0	12.0	34.0
2	30.11726065467637	34.0	31.0	34.0	12.0	34.0
3	29.812508224246923	34.0	31.0	34.0	11.0	34.0
4	29.874859858832448	34.0	31.0	34.0	12.0	34.0
5	30.642770294809644	34.0	32.0	34.0	21.0	34.0
6	33.160463610668096	37.0	34.0	38.0	21.0	38.0
7	33.41611268139399	38.0	34.0	38.0	21.0	38.0
8	32.56636506708354	37.0	31.0	38.0	11.0	38.0
9	33.48668593114266	38.0	34.0	38.0	21.0	38.0
10-11	32.09556706511498	37.0	30.0	38.0	11.5	38.0
12-13	33.025414896808726	37.0	34.0	38.0	16.0	38.0
14-15	31.79101464837068	37.0	29.0	38.0	11.0	38.0
16-17	32.450701890129324	37.0	30.5	38.0	16.0	38.0
18-19	33.32437482564596	37.5	34.0	38.0	21.0	38.0
20-21	32.0309731718486	37.0	30.0	38.0	16.0	38.0
22-23	32.94579892308421	37.5	32.5	38.0	16.0	38.0
24-25	33.25253833157006	38.0	34.0	38.0	16.0	38.0
26-27	32.17350134482886	37.0	30.0	38.0	11.0	38.0
28-29	33.41054651107707	37.5	34.0	38.0	16.0	38.0
30-31	33.8093132688026	38.0	35.0	38.0	21.0	38.0
32-33	33.90720154536889	38.0	35.5	38.0	21.0	38.0
34-35	33.8664987604415	38.0	34.5	38.0	21.0	38.0
36-37	33.36555659071411	38.0	34.0	38.0	16.0	38.0
38-39	33.33008574270871	38.0	34.0	38.0	16.0	38.0
40-41	33.30100217383295	38.0	34.0	38.0	16.0	38.0
42-43	33.68570744314085	38.0	35.0	38.0	16.0	38.0
44-45	33.73212377689	38.0	34.0	38.0	21.0	38.0
46-47	33.66861416833784	38.0	34.0	38.0	21.0	38.0
48-49	33.38357624469043	38.0	34.0	38.0	16.0	38.0
50-51	32.759065093927475	37.5	32.5	38.0	11.0	38.0
52-53	33.32219046566344	37.5	34.0	38.0	21.0	38.0
54-55	33.46988214983131	38.0	34.0	38.0	16.0	38.0
56-57	33.51226662876934	38.0	34.0	38.0	16.0	38.0
58-59	33.758391363619616	38.0	35.0	38.0	21.0	38.0
60-61	33.63941743382442	38.0	34.0	38.0	21.0	38.0
62-63	33.66516656402806	38.0	34.0	38.0	21.0	38.0
64-65	33.688615536852524	38.0	34.5	38.0	21.0	38.0
66-67	33.54283977324764	38.0	34.0	38.0	21.0	38.0
68-69	32.918015443161906	38.0	32.5	38.0	11.0	38.0
70-71	32.993612720870374	37.0	33.5	38.0	11.0	38.0
72-73	33.20434819224474	37.5	34.0	38.0	15.0	38.0
74-75	33.24673003942375	38.0	34.0	38.0	15.0	38.0
76	32.30607883697306	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	29.0
13	301.0
14	1255.0
15	2497.0
16	3509.0
17	4073.0
18	4049.0
19	3518.0
20	2704.0
21	2100.0
22	1746.0
23	1428.0
24	1462.0
25	1551.0
26	1702.0
27	2002.0
28	2407.0
29	2911.0
30	3779.0
31	4784.0
32	6221.0
33	8324.0
34	11686.0
35	17814.0
36	32107.0
37	66027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07573189603445	19.35037318539261	10.08021643700062	28.493678481572328
2	20.02558069762668	20.44455673282909	31.757962386900157	27.771900182644078
3	18.09334322874723	21.379883886792253	32.88803970798002	27.638733176480496
4	12.688762915357366	22.93630616831678	32.403796049203365	31.971134867122487
5	13.188270776421545	33.83863106423071	35.90245648386469	17.07064167548306
6	27.370964176307726	37.150632151842764	17.055993599528385	18.422410072321117
7	26.078854555982524	31.351792388271832	19.941043322629888	22.628309733115756
8	23.004042445679637	35.77406518443658	17.920982819605864	23.300909550277918
9	24.16579372641087	17.43792473588074	19.51613667349227	38.88014486421611
10-11	23.0641081988699	26.187274158018987	24.876107661981173	25.872509981129937
12-13	23.185655785163117	23.031170717842368	26.081658648531995	27.701514848462516
14-15	24.05558333552649	25.19330473458431	22.972603100244758	27.778508829644444
16-17	25.027107260534986	26.46563430989652	23.573052751255354	24.93420567831314
18-19	21.885288830754572	26.400202124943746	25.82962025249827	25.884888791803412
20-21	23.367914646791625	25.77781637691	25.8620326653929	24.992236310905483
22-23	24.97019462318898	25.64157225007567	24.49067677286066	24.897556353874702
24-25	22.08679861038004	28.21533845667965	24.63943573007685	25.05842720286346
26-27	22.89411560355922	28.20557358324714	25.36772543167768	23.532585381515958
28-29	22.29076907285413	27.49275564480683	26.52052501506773	23.695950267271307
30-31	22.67752012653597	27.06180208489595	26.231738737956316	24.028939050611758
32-33	21.349092306315693	28.342728713017205	26.008621642533434	24.299557338133663
34-35	21.736292599991053	28.535710431915025	25.601709658147325	24.1262873099466
36-37	23.369430741935993	26.565288577460265	25.58449899709922	24.480781683504517
38-39	23.163859651893322	26.130899271202633	26.988137631895476	23.717103445008565
40-41	21.88860290440925	26.66550869270003	26.835257149173366	24.61063125371736
42-43	20.53461693330842	26.306869172283292	27.424316990944092	25.734196903464195
44-45	20.39498790665358	24.819652543287336	28.99692336845097	25.78843618160811
46-47	20.661322328839947	25.688614244432394	28.254896490738645	25.39516693598901
48-49	20.430608410049107	26.305220883534137	27.85322153621037	25.410949170206383
50-51	22.11174566413138	26.202858120377925	26.835013290522937	24.85038292496776
52-53	18.707098037564222	27.20537143097785	27.879432325444288	26.20809820601365
54-55	19.131615725351168	26.17838803833166	27.82072236390865	26.869273872408524
56-57	19.868342782541866	26.575964793143932	27.127651174176027	26.428041250138186
58-59	19.47404163025511	26.695111550974428	27.02255235367818	26.808294465092285
60-61	18.988014857049595	25.755553625156956	27.6166333672908	27.63979815050265
62-63	19.76706235359145	25.95083304819309	26.65517332140131	27.626931276814148
64-65	20.313302043491653	25.75015333426343	25.96758526832838	27.96895935391654
66-67	19.76899062379902	27.54581492927049	24.847195826248097	27.83799862068239
68-69	20.212698763599615	28.377204756114892	24.142441324932758	27.26765515535273
70-71	21.002392287832492	28.466382960804903	24.01051659205571	26.520708159306892
72-73	21.572575338860375	28.553230688248455	23.633109619686802	26.241084353204368
74-75	22.652092205488323	28.69972153058163	22.80159185542752	25.846594408502526
76	21.86113348176923	29.19186655507656	23.44287060284976	25.50412936030445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	3.0
15	9.0
16	14.5
17	16.0
18	25.5
19	47.5
20	60.5
21	61.0
22	96.0
23	182.5
24	310.5
25	387.0
26	464.0
27	625.5
28	851.5
29	993.0
30	1227.5
31	1640.0
32	2334.5
33	2851.0
34	3239.5
35	3969.5
36	4895.5
37	5480.0
38	6147.0
39	7610.5
40	8974.0
41	10013.0
42	10485.0
43	10868.0
44	11784.5
45	12166.0
46	12014.0
47	12189.0
48	11947.5
49	11164.0
50	10797.0
51	10350.5
52	9213.5
53	7869.0
54	7215.0
55	6715.0
56	5715.0
57	4656.5
58	4098.0
59	3764.0
60	3119.5
61	2510.0
62	2211.0
63	2049.5
64	1703.0
65	1416.0
66	1189.0
67	1064.0
68	1021.5
69	886.5
70	773.5
71	753.0
72	692.0
73	609.5
74	562.0
75	536.0
76	475.0
77	395.5
78	323.0
79	269.0
80	238.5
81	188.5
82	162.0
83	155.0
84	145.0
85	114.0
86	72.5
87	52.0
88	52.5
89	39.5
90	26.0
91	22.0
92	18.0
93	19.0
94	11.5
95	4.5
96	6.0
97	7.0
98	6.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.263518030181012E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	5.263518030181012E-4
7	0.00894798065130772
8	0.0015790554090543037
9	0.00842162884828962
10-11	0.0018422313105633543
12-13	5.263518030181012E-4
14-15	0.0010527036060362024
16-17	5.263518030181012E-4
18-19	0.0028949349165995567
20-21	0.0
22-23	0.0023685831135814555
24-25	0.0036844626211267086
26-27	7.895277045271518E-4
28-29	0.006053045734708165
30-31	7.895277045271518E-4
32-33	0.0
34-35	0.003947638522635759
36-37	0.020001368514687846
38-39	0.008684804749798672
40-41	0.0
42-43	2.631759015090506E-4
44-45	0.003421286719617658
46-47	0.002105407212072405
48-49	0.0
50-51	0.0010527036060362024
52-53	0.010000684257343923
54-55	0.0071057493407443665
56-57	0.01263244327243443
58-59	0.015264202287524935
60-61	0.022896303431287405
62-63	0.011579739666398227
64-65	0.022369951628269304
66-67	0.020001368514687846
68-69	0.0
70-71	7.895277045271518E-4
72-73	0.006316221636217215
74-75	0.010527036060362024
76	0.0031581108181086073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	189987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.06468147192098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1041863666975	43.76940163484856
2	11.433326989804552	13.506116336342458
3	6.084467587685678	10.78131419978646
4	3.365859730117376	7.952137313541948
5	1.7938423011477893	5.297636206407596
6	0.9174541653331	3.2513482824292006
7	0.57149791542171	2.36287396353751
8	0.38463603960622417	1.817472412957025
9	0.2325697210627359	1.2362990845125605
>10	1.1091775232641614	9.919734091732817
>50	0.0029816598591750603	0.1056664739038726
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010527036060362024	0.0
2	0.0	0.0	0.0	0.0010527036060362024	0.0
3	0.0	0.0	0.0	0.0015790554090543037	0.0
4	0.0	0.0	0.0	0.002105407212072405	0.0
5	0.0	0.0	0.0	0.0031581108181086073	0.0
6	0.0	0.0	0.0	0.0031581108181086073	0.0
7	0.0	0.0	0.0	0.00421081442414481	0.0
8	0.0	0.0	0.0	0.00421081442414481	0.0
9	0.0	0.0	0.0	0.007895277045271518	0.0
10	0.0	0.0	0.0	0.007895277045271518	0.0
11	0.0	0.0	0.0	0.00894798065130772	0.0
12	0.0	0.0	0.0	0.009474332454325822	0.0
13	0.0	0.0	0.0	0.011053387863380126	0.0
14	0.0	0.0	0.0	0.011053387863380126	0.0
15	0.0	0.0	0.0	0.015790554090543037	0.0
16	0.0	0.0	0.0	0.016316905893561138	0.0
17	0.0	0.0	0.0	0.018422313105633543	0.0
18	0.0	0.0	0.0	0.019475016711669745	0.0
19	0.0	0.0	0.0	0.02579123834788696	0.0
20	0.0	0.0	0.0	0.02631759015090506	0.0
21	0.0	0.0	0.0	0.03052840457504987	0.0
22	0.0	0.0	0.0	0.04473990325653861	0.0
23	0.0	0.0	0.0	0.04737166227162911	0.0
24	0.0	0.0	0.0	0.05263518030181012	0.0
25	0.0	0.0	0.0	0.05263518030181012	0.0
26	0.0	0.0	0.0	0.060530457347081644	0.0
27	0.0	0.0	0.0	0.0852689920889324	0.0
28	0.0	0.0	0.0	0.10684941601267454	0.0
29	0.0	0.0	0.0	0.14737850484506834	0.0
30	0.0	0.0	0.0	0.20632990678309568	0.0
31	0.0	0.0	0.0	0.24633264381247139	0.0
32	0.0	0.0	0.0	0.28633538084184706	0.0
33	0.0	0.0	0.0	0.3473921899919468	0.0
34	0.0	0.0	0.0	0.42002873880844477	0.0
35	0.0	0.0	0.0	0.5305626174422461	0.0
36	0.0	0.0	0.0	0.7232073773468711	0.0
37	0.0	0.0	5.263518030181012E-4	1.0927063430655781	0.0
38	0.0	0.0	5.263518030181012E-4	1.590108796917684	0.0
39	0.0	0.0	5.263518030181012E-4	2.1338302094353825	0.0
40	0.0	0.0	5.263518030181012E-4	2.7686104838752126	0.0
41	0.0	0.0	5.263518030181012E-4	3.562349002826509	0.0
42	0.0	0.0	5.263518030181012E-4	4.4250396079731775	0.0
43	0.0	0.0	5.263518030181012E-4	5.369841094390669	0.0
44	0.0	0.0	5.263518030181012E-4	6.243585087400717	0.0
45	0.0	0.0	5.263518030181012E-4	7.145225725970724	0.0
46	0.0	0.0	5.263518030181012E-4	8.028970403238116	0.0
47	0.0	0.0	5.263518030181012E-4	8.921136709353798	0.0
48	0.0	0.0	5.263518030181012E-4	9.748561743698254	0.0
49	0.0	0.0	5.263518030181012E-4	10.512298209877518	0.0
50	0.0	0.0	5.263518030181012E-4	11.270771158026601	0.0
51	0.0	0.0	5.263518030181012E-4	12.054508992720555	0.0
52	0.0	0.0	5.263518030181012E-4	13.049840252227783	0.0
53	0.0	0.0	5.263518030181012E-4	13.991483627827167	0.0
54	0.0	0.0	5.263518030181012E-4	14.867859379852305	0.0
55	0.0	0.0	5.263518030181012E-4	15.675283045682074	0.0
56	0.0	0.0	5.263518030181012E-4	16.455862769557918	0.0
57	0.0	0.0	5.263518030181012E-4	17.18064920231384	0.0
58	0.0	0.0	5.263518030181012E-4	17.96543974061383	0.0
59	0.0	0.0	5.263518030181012E-4	18.62864301241664	0.0
60	0.0	0.0	5.263518030181012E-4	19.26289693505345	0.0
61	0.0	0.0	5.263518030181012E-4	19.936100891113604	0.0
62	0.0	0.0	5.263518030181012E-4	20.581408201613794	0.0
63	0.0	0.0	5.263518030181012E-4	21.309879096990848	0.0
64	0.0	0.0	5.263518030181012E-4	22.03782364056488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCTT	20	7.912055E-5	69.99921	14
CTTCCGA	30	1.0216354E-7	69.98078	18
GCCGTAT	50	9.731593E-10	56.01412	54
CGTATCA	45	2.6822818E-8	54.458168	56
GACGAAT	45	2.6877387E-8	54.44383	23
ACGAATA	45	2.6877387E-8	54.44383	24
GAGCGAC	20	0.0069434587	52.49941	26
ATCTCGA	30	5.8748934E-4	46.653854	42
TCTCGGT	105	0.0	46.653854	43
TAGATCT	160	0.0	45.92489	39
TCGGTGG	115	0.0	45.63964	45
GTGTAGA	195	0.0	44.871292	36
CGCCGTA	95	0.0	44.210026	53
TACCGAC	40	4.5805653E-5	43.749508	19
AGATCTC	185	0.0	43.501568	40
CGACGAG	105	0.0	43.332844	22
GACGATC	65	9.949872E-9	43.076435	23
CGACGAT	75	7.4942363E-10	41.999527	22
TCGCCGT	100	0.0	41.999523	52
GATCTCG	150	0.0	41.988472	41
>>END_MODULE