FastQCFastQC Report
Wed 25 May 2016
SRR1779805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189987
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT175389.231157921331459No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG22521.185344260396764No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC12850.6763620668782601No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4640.24422723660039897RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4360.22948938611589212No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT2720.14316769042092353No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT2550.1342197097696158No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGC150.002224359769.98157540
TCCGGTC207.921939E-569.9815758
CAACTCG800.056.86002736
CTCGTAT27300.053.44746439
CCGTCTT27500.053.3272747
CGTCTTC27450.053.32498648
TATGCCG27450.053.28287543
TCTCGTA25950.053.26150538
GCCGTCT27550.053.2304946
CTGCTTG27050.053.22133654
CATCTCG25800.053.164336
CGTATGC27650.053.15056241
TCGTATG27650.053.15056240
ATGCCGT27550.053.0894744
ATCTCGT26050.053.0570537
TGCCGTC27700.052.94223845
CTTGAAA27000.052.930757
GTCTTCT27650.052.82653849
GTATGCC27800.052.7379142
TCTTCTG27650.052.69985650