##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779805_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189987
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.57782900935327	34.0	34.0	34.0	34.0	34.0
2	33.674104017643316	34.0	34.0	34.0	34.0	34.0
3	33.770305336680934	34.0	34.0	34.0	34.0	34.0
4	33.58743492975835	34.0	34.0	34.0	34.0	34.0
5	33.620510877060006	34.0	34.0	34.0	34.0	34.0
6	37.28590377236337	38.0	38.0	38.0	37.0	38.0
7	37.33892845299942	38.0	38.0	38.0	37.0	38.0
8	37.25818608641644	38.0	38.0	38.0	36.0	38.0
9	37.16087942859248	38.0	38.0	38.0	36.0	38.0
10-11	37.11699747877486	38.0	38.0	38.0	35.5	38.0
12-13	37.2758451894077	38.0	38.0	38.0	36.5	38.0
14-15	37.29536757778164	38.0	38.0	38.0	36.5	38.0
16-17	37.239876938948456	38.0	38.0	38.0	36.0	38.0
18-19	37.11693168479948	38.0	38.0	38.0	36.0	38.0
20-21	37.11387094906493	38.0	38.0	38.0	36.0	38.0
22-23	37.11072336528289	38.0	38.0	38.0	36.0	38.0
24-25	37.292972677077906	38.0	38.0	38.0	36.0	38.0
26-27	37.211956607557354	38.0	38.0	38.0	36.0	38.0
28-29	36.72759451962503	38.0	37.0	38.0	34.0	38.0
30-31	37.00402659129308	38.0	38.0	38.0	35.5	38.0
32-33	37.097409296425546	38.0	38.0	38.0	36.0	38.0
34-35	37.176225215409474	38.0	38.0	38.0	36.0	38.0
36-37	37.12521119866096	38.0	38.0	38.0	36.0	38.0
38-39	36.6052835193987	38.0	37.0	38.0	34.0	38.0
40-41	36.87561517366977	38.0	37.5	38.0	35.0	38.0
42-43	37.195602857037585	38.0	38.0	38.0	36.0	38.0
44-45	36.97174543521399	38.0	38.0	38.0	35.5	38.0
46-47	37.10552300946907	38.0	38.0	38.0	36.0	38.0
48-49	36.77073957691842	38.0	37.0	38.0	34.5	38.0
50-51	36.94262502171201	38.0	37.5	38.0	35.5	38.0
52-53	36.85589803512872	38.0	37.5	38.0	35.0	38.0
54-55	36.86325116981688	38.0	37.5	38.0	35.5	38.0
56-57	36.755025343839314	38.0	37.0	38.0	34.5	38.0
58-59	36.850573986641194	38.0	37.5	38.0	35.5	38.0
60-61	36.52590177222652	38.0	37.0	38.0	34.0	38.0
62-63	36.35937458878765	38.0	37.0	38.0	34.0	38.0
64-65	36.71211977661629	38.0	37.0	38.0	34.5	38.0
66-67	36.50927431876918	38.0	37.0	38.0	34.0	38.0
68-69	36.60135430318917	38.0	37.0	38.0	34.0	38.0
70-71	35.89479016985373	38.0	37.0	38.0	31.0	38.0
72-73	34.60408343728781	38.0	37.0	38.0	23.0	38.0
74-75	34.41228084026802	38.0	36.5	38.0	22.5	38.0
76	34.16114260449399	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	7.0
23	11.0
24	34.0
25	76.0
26	143.0
27	319.0
28	472.0
29	878.0
30	1469.0
31	2444.0
32	3864.0
33	6092.0
34	10571.0
35	20717.0
36	30484.0
37	112404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.388749038897025	17.24350399713512	11.37313967327765	33.994607290690205
2	18.546532131145817	17.897540358024496	39.84535784027328	23.710569670556406
3	17.50593461657903	19.58502423850053	37.25044345139404	25.658597693526396
4	12.544542521330406	24.811697642470275	34.573944533047	28.069815303152325
5	12.246101049019144	32.609599604183444	41.67653576297325	13.467763583824159
6	26.58971403306542	42.90451451941449	17.611731328985666	12.894040118534425
7	25.531746908999036	27.598204087648103	20.051898287777583	26.81815071557528
8	22.615757920278757	41.488101817492776	17.27749793406917	18.618642328159297
9	21.74148757546569	16.201634848700174	18.824445883139372	43.23243169269476
10-11	22.460484138388416	28.16455862769558	28.522741029649396	20.852216204266607
12-13	22.608125819134994	20.717996494496994	29.29358324516941	27.38029444119861
14-15	20.30875796765042	21.210135430318918	21.834388668698384	36.646717933332276
16-17	26.278376941580213	28.850921378831185	22.466274008221614	22.40442767136699
18-19	26.2839036355119	24.636422492065247	23.613984114702586	25.465689757720266
20-21	20.281914025696494	23.78452209888045	23.10473874528257	32.82882513014048
22-23	20.729839410064898	29.211472363898583	22.864722323106317	27.193965902930202
24-25	25.837693303717117	29.523754381901824	23.225132378175235	21.413419936205827
26-27	25.858874554574783	30.125482269839516	23.52660971540158	20.489033460184118
28-29	25.565696600293702	24.825909141151765	24.22534173390811	25.38305252464642
30-31	26.393384810539665	23.45134140756999	29.628079815987405	20.527193965902928
32-33	20.790896219215	29.43411917657524	29.43675093559033	20.33823366861943
34-35	25.185670598514637	29.520967224073225	25.32804876123103	19.965313416181107
36-37	21.37856800728471	28.778548005916193	23.51345092032613	26.32943306647297
38-39	19.72924463252749	24.725639122676814	29.618868659434593	25.926247585361107
40-41	19.552916778516423	22.46153684199445	31.039228999878937	26.946317379610186
42-43	25.432596526595308	22.46685948738463	30.154247801817498	21.946296184202563
44-45	19.506597819850832	27.722159937258866	31.659271423834262	21.11197081905604
46-47	24.763799628395628	22.53391021490944	26.541026491286246	26.161263665408686
48-49	24.056382805139297	22.063878054814275	25.855716443756677	28.024022696289748
50-51	19.27377536785028	22.26158827090627	31.48350925219131	26.981127109052146
52-53	17.64735305499759	22.321266384627737	37.368929559592786	22.66245100078188
54-55	17.34338136164476	22.50326508257499	32.51495618469835	27.6383973710819
56-57	22.77243104469752	22.548350997301032	26.72740438417484	27.951813573826605
58-59	17.676112145202183	22.3906020867285	36.26474018571515	23.668545582354163
60-61	22.989861440565296	27.88617222650769	26.385265258518785	22.738701074408233
62-63	17.28237434219359	34.988061422467695	24.35785721619224	23.371707019146477
64-65	17.23609678030702	35.780391248499654	23.398576512455517	23.584935458737814
66-67	17.285253946356928	35.70729036130622	22.62744705721296	24.380008635123893
68-69	17.19447485892361	36.043438894971786	22.559062578960667	24.203023667143942
70-71	17.904362314529905	34.87982775003751	22.444349100978382	24.771460834454203
72-73	19.00287990228339	32.10274985916383	22.480348747216183	26.414021491336605
74-75	19.48908952120239	31.347950830459848	22.78460688057698	26.378352767760788
76	19.56546917115903	31.57534535040431	22.48410124663073	26.37508423180593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	1.5
14	7.0
15	10.5
16	19.0
17	28.0
18	21.5
19	30.5
20	64.0
21	82.0
22	102.0
23	187.0
24	332.5
25	413.0
26	520.5
27	685.0
28	944.0
29	1146.0
30	1291.5
31	1618.0
32	2378.0
33	2957.0
34	3144.5
35	3749.0
36	4780.0
37	5394.0
38	5970.0
39	7329.0
40	11246.0
41	15866.5
42	17353.0
43	16595.0
44	15009.0
45	13109.0
46	12037.0
47	11531.5
48	10558.5
49	9655.0
50	9219.0
51	8662.0
52	7377.0
53	6319.0
54	5989.0
55	5508.0
56	4423.5
57	3555.5
58	3291.0
59	2817.0
60	2145.5
61	1833.5
62	1719.0
63	1519.0
64	1217.0
65	1059.0
66	980.5
67	958.0
68	874.5
69	722.5
70	628.0
71	602.0
72	564.5
73	510.5
74	482.0
75	470.0
76	449.0
77	381.0
78	304.0
79	274.0
80	222.5
81	161.0
82	151.5
83	152.0
84	120.5
85	89.5
86	70.0
87	50.0
88	36.5
89	17.5
90	12.5
91	7.0
92	1.0
93	2.5
94	2.0
95	2.0
96	4.0
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05316153210482823
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.263518030181012E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.631759015090506E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01684325769657924
52-53	0.031844284082595126
54-55	0.052108828498792024
56-57	0.05237200440030108
58-59	0.03816050571881234
60-61	0.036055098506739935
62-63	0.029738876870522717
64-65	0.016316905893561138
66-67	0.03421286719617658
68-69	0.010000684257343923
70-71	0.01605372999205209
72-73	0.02631759015090506
74-75	0.01684325769657924
76	0.018422313105633543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	189987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.97823008942717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.36600507948453	11.013911478153767
2	18.013357163013826	10.079637027796638
3	14.237606998400903	11.950291335722971
4	9.863606434013732	11.03865001289562
5	6.200733703320478	8.674277713738308
6	3.8096133947888253	6.3951744066699305
7	2.073182202991252	4.060277808481633
8	1.4090866334305334	3.153900003684463
9	0.8879691468347286	2.2359424592208943
>10	4.065468911673408	18.364414407301553
>50	0.05643871695983445	1.0374394037486776
>100	0.011287743391966889	0.9032196939790618
>500	0.0	0.0
>1k	0.00376258113065563	1.8617063272750243
>5k	0.0	0.0
>10k+	0.001881290565327815	9.231157921331459
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	17538	9.231157921331459	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2252	1.185344260396764	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1285	0.6763620668782601	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	464	0.24422723660039897	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	436	0.22948938611589212	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	272	0.14316769042092353	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	255	0.1342197097696158	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015790554090543037	0.0
2	0.0	0.0	0.0	0.0015790554090543037	0.0
3	0.0	0.0	0.0	0.002105407212072405	0.0
4	0.0	0.0	0.0	0.002631759015090506	0.0
5	0.0	0.0	0.0	0.00421081442414481	0.0
6	0.0	0.0	0.0	0.00421081442414481	0.0
7	0.0	0.0	0.0	0.0057898698331991134	0.0
8	0.0	0.0	0.0	0.0057898698331991134	0.0
9	0.0	0.0	0.0	0.011053387863380126	0.0
10	0.0	0.0	0.0	0.012106091469416328	0.0
11	0.0	0.0	0.0	0.01315879507545253	0.0
12	0.0	0.0	0.0	0.015264202287524935	0.0
13	0.0	0.0	0.0	0.01684325769657924	0.0
14	0.0	0.0	0.0	0.01684325769657924	0.0
15	0.0	0.0	0.0	0.022633127529778353	0.0
16	0.0	0.0	0.0	0.023159479332796454	0.0
17	0.0	0.0	0.0	0.02526488654486886	0.0
18	0.0	0.0	0.0	0.02631759015090506	0.0
19	0.0	0.0	0.0	0.03158110818108607	0.0
20	0.0	0.0	0.0	0.03210745998410418	0.0
21	0.0	0.0	0.0	0.03631827440824899	0.0
22	0.0	0.0	0.0	0.03737097801428519	0.0
23	0.0	0.0	0.0	0.0421081442414481	0.0
24	0.0	0.0	0.0	0.0426344960444662	0.0
25	0.0	0.0	0.0	0.0426344960444662	0.0
26	0.0	0.0	0.0	0.052108828498792024	0.0
27	0.0	0.0	0.0	0.08211088127082379	0.0
28	0.0	0.0	0.0	0.10474400880060214	0.0
29	0.0	0.0	0.0	0.14369404222394164	0.0
30	0.0	0.0	0.0	0.20211909235895087	0.0
31	0.0	0.0	0.0	0.24422723660039897	0.0
32	0.0	0.0	0.0	0.2847563254327928	0.0
33	0.0	0.0	0.0	0.34949759720401924	0.0
34	0.0	0.0	0.0	0.43002942306578873	0.0
35	0.0	0.0	0.0	0.5437214125176986	0.0
36	0.0	0.0	0.0	0.7526830783158848	0.0
37	0.0	0.0	0.0	1.1474469305794608	0.0
38	0.0	0.0	0.0	1.6543237168858922	0.0
39	0.0	0.0	0.0	2.204887702842826	0.0
40	0.0	0.0	0.0	2.8723017890697786	0.0
41	0.0	0.0	0.0	3.677620047687473	0.0
42	0.0	0.0	0.0	4.570312705606173	0.0
43	0.0	0.0	0.0	5.549327059219841	0.0
44	0.0	0.0	0.0	6.4546521604109754	0.0
45	0.0	0.0	0.0	7.368925242253417	0.0
46	0.0	0.0	0.0	8.287409138520005	0.0
47	0.0	0.0	0.0	9.201682220362446	0.0
48	0.0	0.0	0.0	10.046476864206499	0.0
49	0.0	0.0	0.0	10.843373493975903	0.0
50	0.0	0.0	0.0	11.618689699821566	0.0
51	0.0	0.0	0.0	12.41611268139399	0.0
52	0.0	0.0	0.0	13.411970292704238	0.0
53	0.0	0.0	0.0	14.35150826109155	0.0
54	0.0	0.0	0.0	15.22946306852574	0.0
55	0.0	0.0	0.0	16.032149568128347	0.0
56	0.0	0.0	0.0	16.808518477580044	0.0
57	0.0	0.0	0.0	17.545411001805387	0.0
58	0.0	0.0	0.0	18.321779911257085	0.0
59	0.0	0.0	0.0	19.000773737150435	0.0
60	0.0	0.0	0.0	19.628711438151033	0.0
61	0.0	0.0	0.0	20.30507350502929	0.0
62	0.0	0.0	0.0	20.971961239453226	0.0
63	0.0	0.0	0.0	21.715169985314784	0.0
64	0.0	0.0	0.0	22.467326711827653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGC	15	0.0022243597	69.981575	40
TCCGGTC	20	7.921939E-5	69.981575	8
CAACTCG	80	0.0	56.860027	36
CTCGTAT	2730	0.0	53.447464	39
CCGTCTT	2750	0.0	53.32727	47
CGTCTTC	2745	0.0	53.324986	48
TATGCCG	2745	0.0	53.282875	43
TCTCGTA	2595	0.0	53.261505	38
GCCGTCT	2755	0.0	53.23049	46
CTGCTTG	2705	0.0	53.221336	54
CATCTCG	2580	0.0	53.1643	36
CGTATGC	2765	0.0	53.150562	41
TCGTATG	2765	0.0	53.150562	40
ATGCCGT	2755	0.0	53.08947	44
ATCTCGT	2605	0.0	53.05705	37
TGCCGTC	2770	0.0	52.942238	45
CTTGAAA	2700	0.0	52.9307	57
GTCTTCT	2765	0.0	52.826538	49
GTATGCC	2780	0.0	52.73791	42
TCTTCTG	2765	0.0	52.699856	50
>>END_MODULE