FastQCFastQC Report
Wed 25 May 2016
SRR1779804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248796
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT150.002224267569.9887514
CGACGAT1300.056.5293722
GACGAAT700.055.00221323
GGACGGT200.006939039652.5126758
CGTAACC200.006939039652.5126756
TGTGCGA200.006944560452.50211310
CCGTCAT200.006944560452.50211370
GTGTCGA200.006944560452.50211336
TCGACGT200.006944560452.50211352
GCCGTAT608.367351E-1152.50211354
GTGCGAA200.006950084652.49155811
CGCCGTA800.048.12693453
ACGAACG451.679964E-646.66854524
TTACGCT451.6822923E-646.65916413
TCGCCGT1000.045.5018352
GACGATC703.8198777E-1045.0018123
CGGCGGT551.3087265E-744.53829646
TGGTCGC551.3087265E-744.53829649
TCTCGGT1300.043.06999643
GTAGATC2300.042.60184538
Produced by FastQC (version 0.11.5)