##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779804_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248796
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.068385343815816	34.0	24.0	34.0	12.0	34.0
2	29.81728002057911	34.0	31.0	34.0	12.0	34.0
3	29.409729256097364	34.0	27.0	34.0	11.0	34.0
4	29.611022685252173	34.0	27.0	34.0	12.0	34.0
5	30.10883213556488	34.0	32.0	34.0	11.0	34.0
6	32.68832698274892	37.0	32.0	38.0	12.0	38.0
7	32.94159472017235	38.0	34.0	38.0	12.0	38.0
8	32.272725445746715	37.0	31.0	38.0	11.0	38.0
9	33.086858309619124	38.0	34.0	38.0	12.0	38.0
10-11	31.73604680139552	37.0	28.5	38.0	11.5	38.0
12-13	32.568887763468865	37.0	31.5	38.0	11.0	38.0
14-15	31.374310680236015	37.0	27.0	38.0	11.0	38.0
16-17	31.99177237576167	37.0	30.0	38.0	11.0	38.0
18-19	32.80861629608194	37.5	33.5	38.0	11.0	38.0
20-21	31.603621038923457	37.0	27.5	38.0	11.0	38.0
22-23	32.469955304747664	37.5	32.5	38.0	11.0	38.0
24-25	32.74549831990868	37.5	32.5	38.0	11.0	38.0
26-27	31.66715702824804	37.0	29.0	38.0	11.0	38.0
28-29	32.83102622228653	37.5	32.5	38.0	11.0	38.0
30-31	33.188087830994064	38.0	34.0	38.0	11.0	38.0
32-33	33.307115066158616	38.0	34.0	38.0	11.0	38.0
34-35	33.24771499541793	38.0	34.0	38.0	11.0	38.0
36-37	32.778879081657266	38.0	32.5	38.0	11.0	38.0
38-39	32.69919331500506	38.0	32.5	38.0	11.0	38.0
40-41	32.72347827135485	38.0	33.0	38.0	11.0	38.0
42-43	33.06778244023216	38.0	34.0	38.0	11.0	38.0
44-45	33.15578224730301	38.0	34.0	38.0	11.0	38.0
46-47	33.065312545217765	38.0	34.0	38.0	11.0	38.0
48-49	32.791433543947655	38.0	33.0	38.0	11.0	38.0
50-51	32.19669930384733	37.5	30.5	38.0	11.0	38.0
52-53	32.73679842119648	37.5	33.0	38.0	11.0	38.0
54-55	32.86113723693307	38.0	33.0	38.0	11.0	38.0
56-57	32.90698001575588	38.0	34.0	38.0	11.0	38.0
58-59	33.11242946028071	38.0	34.0	38.0	11.0	38.0
60-61	33.03590290840688	38.0	34.0	38.0	11.0	38.0
62-63	33.01295639801283	38.0	34.0	38.0	11.0	38.0
64-65	33.021308622325115	38.0	34.0	38.0	11.0	38.0
66-67	32.91544076271323	38.0	33.5	38.0	11.0	38.0
68-69	32.335190276371	37.0	31.0	38.0	11.0	38.0
70-71	32.36429645171144	37.0	31.0	38.0	11.0	38.0
72-73	32.57981438608338	37.0	32.0	38.0	11.0	38.0
74-75	32.60982290712069	37.5	32.0	38.0	11.0	38.0
76	31.66199617357192	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	50.0
13	734.0
14	2363.0
15	4108.0
16	5094.0
17	6053.0
18	6342.0
19	5730.0
20	4374.0
21	3505.0
22	2598.0
23	2232.0
24	2185.0
25	2241.0
26	2395.0
27	2797.0
28	3234.0
29	3948.0
30	4801.0
31	6056.0
32	7617.0
33	10269.0
34	14268.0
35	21395.0
36	38869.0
37	85537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67668290486985	18.27802697792569	11.890464476920851	30.154825640283605
2	20.04011318509944	22.392241032814034	32.2111287962829	25.35651698580363
3	19.065017122461775	20.393816620845996	30.776218267174716	29.764947989517516
4	13.869194038489367	23.448930047106867	29.398382610652906	33.283493303750866
5	13.84990112381228	35.335375166803324	32.3401501631859	18.474573546198492
6	29.81325766101804	36.40912891089746	16.652062767291554	17.12555066079295
7	26.827425144405275	28.72187765044477	20.312405789877765	24.13829141527219
8	23.201868272330437	38.45393229522562	17.635236709461143	20.708962722982804
9	24.665265155017106	18.870161870575895	18.684454877622308	37.7801180967847
10-11	23.468977603421386	26.50951814396193	23.97925141083975	26.042252841776936
12-13	23.559663338638885	23.053023360504188	25.34526278557533	28.0420505152816
14-15	24.579222694944242	24.996633781559556	22.067119381178518	28.357024142317687
16-17	25.44454090901783	25.95017604784643	22.301202591681538	26.304080451454205
18-19	22.172425851417454	25.86890201012094	25.076670779891558	26.882001358570047
20-21	24.73311468030033	25.0317529220727	24.40855158443062	25.826580813196355
22-23	25.5679443068909	25.080790379111868	22.937232708447215	26.41403260555002
24-25	22.798544957594757	27.6556533622734	22.878130149925642	26.6676715302062
26-27	23.76268863383783	27.602589275127563	23.79946582635136	24.835256264683245
28-29	22.75544657930702	27.175817991799985	24.633813007476483	25.434922421416513
30-31	23.252584446695284	26.659793565812954	24.577766523577548	25.50985546391421
32-33	22.015313812576622	27.41152354347957	24.761550674249886	25.811611969693928
34-35	21.922725217303924	27.976084007436064	24.972717680751646	25.128473094508365
36-37	23.796965579346	25.801218914072987	24.89266159053814	25.50915391604287
38-39	23.809763078135173	25.082605095387784	25.852782043139555	25.254849783337484
40-41	21.986595255082047	26.439502798516834	25.688274365183837	25.88562758121728
42-43	20.906452727959675	25.70379510759906	26.414021238454303	26.975730925986962
44-45	20.444003183510063	24.18945100529781	28.001020974186236	27.365524837005893
46-47	20.958649449831686	25.36843691905743	27.319298598201275	26.353615032909612
48-49	21.097733269827106	25.631997491905974	26.902523970586167	26.36774526768076
50-51	22.680281842992713	25.680591339718877	25.879151577026498	25.759975240261905
52-53	18.748140823102837	27.107000152753997	26.825611216966948	27.31924780717622
54-55	19.618365410898335	25.997298731368485	26.67020404225556	27.71413181547763
56-57	19.996221325792067	26.496947916603858	26.010745102295544	27.496085655308534
58-59	19.686009514984995	26.392526294843936	26.070536308290336	27.85092788188073
60-61	19.328641583696673	25.294239440045512	26.503762043464015	28.873356932793804
62-63	20.212126632806264	25.963905911768663	25.694979026516833	28.128988428908237
64-65	20.675722940729514	25.187845992417817	25.218801886299403	28.917629180553266
66-67	20.206350929242493	27.587627788431	23.698598196590165	28.507423085736338
68-69	20.814207738128733	28.234603728385732	23.03150397517625	27.91968455830928
70-71	21.10709397878981	28.86225836078225	22.731721433868376	27.298926226559566
72-73	21.958586981206775	29.02649460481176	22.35390585903978	26.66101255494169
74-75	22.793689076474728	28.95347201286303	22.14872877097779	26.104110139684455
76	21.768559829575143	29.674022267776035	22.23642429358093	26.320993609067887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	13.5
15	25.5
16	36.0
17	44.0
18	80.0
19	129.0
20	188.0
21	234.0
22	305.5
23	431.0
24	666.5
25	848.0
26	948.5
27	1188.0
28	1592.5
29	1858.0
30	2187.5
31	2854.0
32	3610.5
33	4030.0
34	4406.5
35	5334.0
36	6361.5
37	6838.0
38	7390.5
39	8512.5
40	9678.0
41	10700.0
42	11126.0
43	11505.5
44	12318.5
45	12820.5
46	12889.0
47	13052.5
48	12971.0
49	12653.0
50	12580.0
51	12252.5
52	11392.0
53	10443.5
54	10028.0
55	9667.0
56	8895.0
57	7955.5
58	7427.0
59	6902.0
60	5887.0
61	5091.5
62	4786.0
63	4330.0
64	3605.5
65	3202.0
66	2719.5
67	2372.0
68	2233.0
69	1957.5
70	1663.5
71	1506.0
72	1433.5
73	1227.0
74	1051.5
75	1010.0
76	941.0
77	842.5
78	717.0
79	621.0
80	576.5
81	467.0
82	364.0
83	326.0
84	287.5
85	218.0
86	155.0
87	123.0
88	98.5
89	62.5
90	43.5
91	38.5
92	41.0
93	34.5
94	23.5
95	16.5
96	14.0
97	13.0
98	9.0
99	10.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.00160774288975707
7	0.006832907281467547
8	0.004019357224392675
9	0.006832907281467547
10-11	0.00321548577951414
12-13	0.0
14-15	2.0096786121963375E-4
16-17	0.0
18-19	0.0020096786121963374
20-21	0.0
22-23	0.00160774288975707
24-25	0.002411614334635605
26-27	2.0096786121963375E-4
28-29	0.00643097155902828
30-31	0.0
32-33	4.019357224392675E-4
34-35	0.0034164536407337736
36-37	0.020096786121963375
38-39	0.008842585893663884
40-41	0.0014067750285374362
42-43	8.03871444878535E-4
44-45	0.005627100114149745
46-47	0.0034164536407337736
48-49	6.029035836589013E-4
50-51	0.0020096786121963374
52-53	0.012058071673178026
54-55	0.00964645733854242
56-57	0.012660975256836926
58-59	0.012259039534397657
60-61	0.024719046930014952
62-63	0.011857103811958392
64-65	0.022106464734159715
66-67	0.01808710750976704
68-69	8.03871444878535E-4
70-71	2.0096786121963375E-4
72-73	0.0038183893631730413
74-75	0.008440650171224617
76	0.002411614334635605
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248796.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.47611211566311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41050374041197	49.266522059810974
2	9.273179656663642	11.957971424234625
3	6.088058797018528	11.776030846899472
4	3.830878802472376	9.880006846789046
5	2.1592922455970673	6.961138445879925
6	1.1572930743134429	4.477065480606838
7	0.5471028690139458	2.4692546144940883
8	0.26441407000262634	1.3638712977958505
9	0.11992238621360926	0.6958916296817872
>10	0.14871343936318787	1.1270397377438652
>50	6.409189295682145E-4	0.0252076160635335
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00160774288975707	0.0
2	0.0	0.0	0.0	0.004421292946831943	0.0
3	0.0	0.0	0.0	0.0056271001141497455	0.0
4	0.0	0.0	0.0	0.00964645733854242	0.0
5	0.0	0.0	0.0	0.010450328783420955	0.0
6	0.0	0.0	0.0	0.010450328783420955	0.0
7	0.0	0.0	0.0	0.010450328783420955	0.0
8	0.0	0.0	0.0	0.010852264505860222	0.0
9	0.0	0.0	0.0	0.01446968600781363	0.0
10	0.0	0.0	0.0	0.02090065756684191	0.0
11	0.0	0.0	0.0	0.021704529011720444	0.0
12	0.0	0.0	0.0	0.022508400456598982	0.0
13	0.0	0.0	0.0	0.027331629125870192	4.019357224392675E-4
14	0.0	0.0	0.0	0.027331629125870192	4.019357224392675E-4
15	0.0	0.0	0.0	0.028135500570748727	4.019357224392675E-4
16	0.0	0.0	0.0	0.029743243460505796	4.019357224392675E-4
17	0.0	0.0	0.0	0.030145179182945063	4.019357224392675E-4
18	0.0	0.0	0.0	0.034164536407337735	4.019357224392675E-4
19	0.0	0.0	0.0	0.03898776507660895	4.019357224392675E-4
20	0.0	0.0	0.0	0.042605186578562354	4.019357224392675E-4
21	0.0	0.0	0.0	0.0458206723580765	4.019357224392675E-4
22	0.0	0.0	0.0	0.049438093860029904	4.019357224392675E-4
23	0.0	0.0	0.0	0.050643901027347706	4.019357224392675E-4
24	0.0	0.0	0.0	0.06109422981076866	4.019357224392675E-4
25	0.0	0.0	0.0	0.07033875142687182	4.019357224392675E-4
26	0.0	0.0	0.0	0.08320069454492837	4.019357224392675E-4
27	0.0	0.0	0.0	0.12942330262544413	4.019357224392675E-4
28	0.0	0.0	0.0	0.160774288975707	4.019357224392675E-4
29	0.0	0.0	0.0	0.19574269682792328	4.019357224392675E-4
30	0.0	0.0	0.0	0.2339265904596537	4.019357224392675E-4
31	0.0	0.0	0.0	0.28497242720944066	4.019357224392675E-4
32	0.0	0.0	0.0	0.34204729979581666	4.019357224392675E-4
33	0.0	0.0	0.0	0.40072991527194973	4.019357224392675E-4
34	0.0	0.0	0.0	0.4730783453110179	4.019357224392675E-4
35	0.0	0.0	0.0	0.6105403623852473	4.019357224392675E-4
36	0.0	0.0	0.0	0.838437917008312	4.019357224392675E-4
37	0.0	0.0	0.0	1.2644897827939356	4.019357224392675E-4
38	0.0	0.0	0.0	1.825592051319153	4.019357224392675E-4
39	0.0	0.0	0.0	2.438544028039036	4.019357224392675E-4
40	0.0	0.0	0.0	3.039035997363302	4.019357224392675E-4
41	0.0	0.0	0.0	3.893953278991624	4.019357224392675E-4
42	0.0	0.0	0.0	4.800720268814612	4.019357224392675E-4
43	0.0	0.0	0.0	5.76737568128105	4.019357224392675E-4
44	0.0	0.0	0.0	6.696651071560636	4.019357224392675E-4
45	0.0	0.0	0.0	7.579703853759707	4.019357224392675E-4
46	0.0	0.0	0.0	8.508577308316854	4.019357224392675E-4
47	0.0	0.0	0.0	9.324908760591006	4.019357224392675E-4
48	0.0	0.0	0.0	10.081753725944147	4.019357224392675E-4
49	0.0	0.0	0.0	10.761427032588948	4.019357224392675E-4
50	0.0	0.0	0.0	11.490940368816219	4.019357224392675E-4
51	0.0	0.0	0.0	12.244569848389846	4.019357224392675E-4
52	0.0	0.0	0.0	13.11998585186257	4.019357224392675E-4
53	0.0	0.0	0.0	13.96525667615235	4.019357224392675E-4
54	0.0	0.0	0.0	14.751442949243557	4.019357224392675E-4
55	0.0	0.0	0.0	15.510297593208895	4.019357224392675E-4
56	0.0	0.0	0.0	16.226948986318106	4.019357224392675E-4
57	0.0	0.0	0.0	16.956462322545377	4.019357224392675E-4
58	0.0	0.0	0.0	17.562179456261354	4.019357224392675E-4
59	0.0	0.0	0.0	18.196835961992956	4.019357224392675E-4
60	0.0	0.0	0.0	18.77843695236258	4.019357224392675E-4
61	0.0	0.0	0.0	19.29813984147655	4.019357224392675E-4
62	0.0	0.0	0.0	19.90265116802521	4.019357224392675E-4
63	0.0	0.0	0.0	20.597598032122704	4.019357224392675E-4
64	0.0	0.0	0.0	21.203717101561118	4.019357224392675E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTT	15	0.0022242675	69.98875	14
CGACGAT	130	0.0	56.52937	22
GACGAAT	70	0.0	55.002213	23
GGACGGT	20	0.0069390396	52.51267	58
CGTAACC	20	0.0069390396	52.51267	56
TGTGCGA	20	0.0069445604	52.502113	10
CCGTCAT	20	0.0069445604	52.502113	70
GTGTCGA	20	0.0069445604	52.502113	36
TCGACGT	20	0.0069445604	52.502113	52
GCCGTAT	60	8.367351E-11	52.502113	54
GTGCGAA	20	0.0069500846	52.491558	11
CGCCGTA	80	0.0	48.126934	53
ACGAACG	45	1.679964E-6	46.668545	24
TTACGCT	45	1.6822923E-6	46.659164	13
TCGCCGT	100	0.0	45.50183	52
GACGATC	70	3.8198777E-10	45.00181	23
CGGCGGT	55	1.3087265E-7	44.538296	46
TGGTCGC	55	1.3087265E-7	44.538296	49
TCTCGGT	130	0.0	43.069996	43
GTAGATC	230	0.0	42.601845	38
>>END_MODULE