FastQCFastQC Report
Wed 25 May 2016
SRR1779804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248796
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2881011.579768163475297No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG38581.5506680171706941No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC19260.7741282014180292No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT7820.3143137349475072TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5810.23352465473721443No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3290.132236852682519No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT2970.11937490956446244No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2690.10812070933616297TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG150.002224546969.986539
ATCGTAT750.055.98922739
ATATCGT700.054.98941837
TCTCGTA39200.052.66843838
CGTCTTC40050.052.6202748
CCGTCTT40050.052.6202747
ATCTCGT39350.052.55659537
CTGCTTG40200.052.5321454
CGTATGC40650.052.5114241
TCCTCGT200.00695095352.48990231
TAGGGCG200.00695095352.48990225
ATCGGTA200.00695095352.48990222
CGGAGTT200.00695095352.48990229
AGCACGT200.00695095352.48990230
TCGTATG40850.052.4256540
GCCGTCT40400.052.42435546
TGCCGTC40600.052.32803745
TATGCCG40550.052.2957343
AATCTCG39050.052.24346536
CTCGTAT40350.052.12132339