##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779804_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248796
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.582987668612034	34.0	34.0	34.0	34.0	34.0
2	33.67519574269683	34.0	34.0	34.0	34.0	34.0
3	33.771985884017425	34.0	34.0	34.0	34.0	34.0
4	33.580214312127204	34.0	34.0	34.0	34.0	34.0
5	33.613731732021414	34.0	34.0	34.0	34.0	34.0
6	37.293919516390936	38.0	38.0	38.0	37.0	38.0
7	37.362433479637936	38.0	38.0	38.0	37.0	38.0
8	37.265856364250226	38.0	38.0	38.0	36.0	38.0
9	37.182249714625634	38.0	38.0	38.0	36.0	38.0
10-11	37.133979244039296	38.0	38.0	38.0	35.5	38.0
12-13	37.291403398768466	38.0	38.0	38.0	36.5	38.0
14-15	37.30436381613852	38.0	38.0	38.0	36.5	38.0
16-17	37.23045587549639	38.0	38.0	38.0	36.0	38.0
18-19	37.10709778292255	38.0	38.0	38.0	36.0	38.0
20-21	37.1206008135179	38.0	38.0	38.0	36.0	38.0
22-23	37.11353478351742	38.0	38.0	38.0	36.0	38.0
24-25	37.28292456470361	38.0	38.0	38.0	36.0	38.0
26-27	37.211142864033185	38.0	38.0	38.0	36.5	38.0
28-29	36.73405922924806	38.0	37.0	38.0	34.0	38.0
30-31	36.96108458335343	38.0	37.5	38.0	36.0	38.0
32-33	37.0335817296098	38.0	38.0	38.0	36.0	38.0
34-35	37.04253886718436	38.0	38.0	38.0	36.0	38.0
36-37	37.03668065402981	38.0	38.0	38.0	36.0	38.0
38-39	36.54202840881686	38.0	37.0	38.0	34.0	38.0
40-41	36.86425625813919	38.0	37.5	38.0	35.0	38.0
42-43	37.18594551359347	38.0	38.0	38.0	36.0	38.0
44-45	36.95128740011897	38.0	38.0	38.0	35.5	38.0
46-47	37.08718588723291	38.0	38.0	38.0	36.0	38.0
48-49	36.74181859836975	38.0	37.0	38.0	34.5	38.0
50-51	36.91477354941398	38.0	37.5	38.0	35.5	38.0
52-53	36.825503625460215	38.0	37.5	38.0	35.0	38.0
54-55	36.817280020579105	38.0	37.5	38.0	35.0	38.0
56-57	36.70593980610621	38.0	37.0	38.0	34.5	38.0
58-59	36.79057340150163	38.0	37.0	38.0	35.0	38.0
60-61	36.46412120773646	38.0	37.0	38.0	34.0	38.0
62-63	36.286208379555944	38.0	37.0	38.0	34.0	38.0
64-65	36.64986977282593	38.0	37.0	38.0	34.0	38.0
66-67	36.42121456936607	38.0	37.0	38.0	34.0	38.0
68-69	36.48073120146626	38.0	37.0	38.0	34.0	38.0
70-71	35.56805977588064	38.0	37.0	38.0	29.5	38.0
72-73	33.965041640540846	38.0	35.5	38.0	21.5	38.0
74-75	33.72440473319507	38.0	35.5	38.0	19.0	38.0
76	33.506173732696666	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	20.0
23	18.0
24	51.0
25	125.0
26	234.0
27	430.0
28	742.0
29	1372.0
30	2120.0
31	3439.0
32	5377.0
33	8760.0
34	14722.0
35	28930.0
36	41377.0
37	141074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.25123865903095	15.1088250434335	10.60018660317869	36.039749694356864
2	19.077879065579832	18.55335294779659	39.10513030756121	23.263637679062366
3	19.01879451438126	17.821829932957122	38.58140806122285	24.57796749143877
4	13.494589945175967	26.581215132076082	31.636762648917184	28.28743227383077
5	13.38928278589688	29.39999035354266	42.049309474428846	15.161417386131609
6	26.501631859033104	42.384925802665634	17.546503963086224	13.566938375215035
7	25.140275567131305	26.724706184986896	18.37770703709063	29.757311210791173
8	22.553819193234617	41.79608996929211	17.027203009694688	18.62288782777858
9	21.622936060065275	17.123667583080113	17.11482499718645	44.13857135966816
10-11	22.265631280245664	28.360182639592274	28.216490618820238	21.15769546134182
12-13	22.499959806427754	19.313413398929242	29.375472274473868	28.811154520169136
14-15	20.718379716715702	19.630540683933827	20.991696007974404	38.65938359137607
16-17	27.91363205196225	28.40298879403206	20.28690171867715	23.396477435328542
18-19	27.72210968021994	23.399692921108056	21.862489750639078	27.015707648032926
20-21	20.889403366613614	21.682020611263848	21.430810784739304	35.99776523738324
22-23	20.538111545201694	29.1700831203074	21.38338236949147	28.908422964999435
24-25	28.031134316479967	28.859332859043462	21.20959994855161	21.899932875924964
26-27	28.04345729031013	29.74645894628531	21.4655782247303	20.744505538674254
28-29	20.662510651296646	23.02388302062734	28.305720349201756	28.00788597887426
30-31	20.141400987154135	29.115620829916878	23.461189086641266	27.281789096287724
32-33	19.961333783501342	22.620942458881977	35.77770542934774	21.640018328268944
34-35	27.386694319844366	28.135701538609947	24.505217125677262	19.972387015868424
36-37	20.508167333879964	36.92322223829965	22.0469782472387	20.52163218058168
38-39	19.93560989726523	23.406726796250744	29.640749851283783	27.016913455200243
40-41	19.953697004774995	21.00094052959051	30.516366822617723	28.52899564301677
42-43	27.01020527104862	20.604076481251482	30.29004031463908	22.095677933060816
44-45	19.837135645267608	27.44798951751636	31.546930014952007	21.167944822264023
46-47	26.236153314361964	20.902265309731668	25.5128699818325	27.34871139407386
48-49	25.36897699319925	20.202897152687342	24.35830961912571	30.0698162349877
50-51	19.399928041213492	20.453179553252518	31.86930033145262	28.27759207408137
52-53	17.25665140104621	21.13229623613328	38.69050730377512	22.920545059045384
54-55	17.11572220557688	21.44809100286948	32.61452273563602	28.821664055917616
56-57	24.3540142609521	20.942003048449433	25.501003414424233	29.202979276174233
58-59	17.385727354061743	21.128301014304814	37.503141429677214	23.982830201956233
60-61	24.385708139492802	27.736116863221298	25.553576382885474	22.324598614400422
62-63	17.271923123316313	36.95167062040127	22.531462345703833	23.244943910578584
64-65	17.3135174357253	37.7065523915958	21.818927344612103	23.161002828066803
66-67	17.34992370279676	37.51932560912108	21.160360838525364	23.970389849556792
68-69	17.485584276460315	37.895515398545264	20.88144436605989	23.737455958934532
70-71	17.360910114166263	36.701238141180255	21.14789355201801	24.78995819263547
72-73	17.964726645573943	33.45259967114653	21.81066401868595	26.772009664593575
74-75	18.57782583702822	32.07378792203146	22.40691088448227	26.94147535645805
76	18.01090181134078	32.98400906876341	22.103502890266356	26.901586229629448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	2.5
14	9.0
15	18.5
16	38.0
17	56.0
18	78.5
19	123.0
20	200.0
21	255.0
22	312.5
23	460.5
24	703.5
25	856.0
26	929.0
27	1233.5
28	1699.5
29	1934.0
30	2238.5
31	2705.5
32	3375.5
33	3883.0
34	4244.5
35	4934.5
36	5666.0
37	6069.0
38	11008.0
39	18384.5
40	19864.0
41	16811.5
42	14717.0
43	13437.0
44	11653.5
45	10904.0
46	10658.0
47	10589.0
48	10225.0
49	9642.5
50	9355.0
51	9292.5
52	8796.0
53	8192.0
54	8022.0
55	7707.5
56	7065.0
57	6324.0
58	5911.0
59	5571.0
60	4858.5
61	4293.5
62	4101.0
63	3693.0
64	3087.0
65	2691.0
66	2364.0
67	2235.0
68	2110.0
69	1844.5
70	1522.5
71	1341.0
72	1265.0
73	1155.0
74	1039.0
75	957.0
76	890.0
77	732.0
78	641.0
79	641.0
80	541.5
81	441.5
82	384.0
83	327.0
84	285.0
85	194.0
86	116.5
87	88.0
88	80.5
89	58.0
90	32.0
91	13.5
92	6.0
93	9.5
94	9.5
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056271001141497454
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0012058071673178026
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0012058071673178026
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017082268203668868
52-53	0.035772279297094804
54-55	0.058079711892474156
56-57	0.05828067975369379
58-59	0.04160034727246419
60-61	0.038183893631730415
62-63	0.03456647212977701
64-65	0.015876461036351066
66-67	0.03918873293782858
68-69	0.00904355375488352
70-71	0.01446968600781363
72-73	0.02130259328928118
74-75	0.011656135950738758
76	0.012058071673178026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.801893921124133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.6585224808443	9.913744593964534
2	15.77273384415209	8.770237463624817
3	14.069683388752349	11.734915352336854
4	11.856296082116524	13.185099438897732
5	8.726326442099177	12.130420103217093
6	5.8609223651872195	9.776684512612743
7	3.6431979181726177	7.0901461438286795
8	1.901113199363886	4.228363800061094
9	1.0640451062599392	2.6624222254377083
>10	1.4052334827237243	4.87306869885368
>50	0.017348561515107705	0.33240084245727425
>100	0.017348561515107705	0.8500940529590507
>500	0.0028914269191846176	0.5478383896847216
>1k	0.0028914269191846176	2.324796218588723
>5k	0.0	0.0
>10k+	0.0014457134595923088	11.579768163475297
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	28810	11.579768163475297	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	3858	1.5506680171706941	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	1926	0.7741282014180292	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	782	0.3143137349475072	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	581	0.23352465473721443	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	329	0.132236852682519	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	297	0.11937490956446244	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	269	0.10812070933616297	TruSeq Adapter, Index 12 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002411614334635605	0.0
2	0.0	0.0	0.0	0.0056271001141497455	0.0
3	0.0	0.0	0.0	0.006832907281467548	0.0
4	0.0	0.0	0.0	0.011656135950738758	0.0
5	0.0	0.0	0.0	0.012460007395617293	0.0
6	0.0	0.0	0.0	0.012460007395617293	0.0
7	0.0	0.0	0.0	0.012460007395617293	0.0
8	0.0	0.0	0.0	0.012460007395617293	0.0
9	0.0	0.0	0.0	0.017283236064888505	0.0
10	0.0	0.0	0.0	0.024920014791234586	0.0
11	0.0	0.0	0.0	0.025321950513673853	0.0
12	0.0	0.0	0.0	0.026125821958552387	0.0
13	0.0	0.0	0.0	0.030949050627823597	0.0
14	0.0	0.0	0.0	0.030949050627823597	0.0
15	0.0	0.0	0.0	0.032556793517580666	0.0
16	0.0	0.0	0.0	0.034164536407337735	0.0
17	0.0	0.0	0.0	0.03456647212977701	0.0
18	0.0	0.0	0.0	0.03898776507660895	0.0
19	0.0	0.0	0.0	0.045016800913197964	0.0
20	0.0	0.0	0.0	0.049438093860029904	0.0
21	0.0	0.0	0.0	0.053457451084422576	0.0
22	0.0	0.0	0.0	0.05506519397417965	0.0
23	0.0	0.0	0.0	0.057476808308815255	0.0
24	0.0	0.0	0.0	0.06069229408832939	0.0
25	0.0	0.0	0.0	0.07194649431662889	0.0
26	0.0	0.0	0.0	0.08641618032444251	0.0
27	0.0	0.0	0.0	0.13505040273959387	0.0
28	0.0	0.0	0.0	0.166803324812296	0.0
29	0.0	0.0	0.0	0.20458528272158716	0.0
30	0.0	0.0	0.0	0.24638659785527098	0.0
31	0.0	0.0	0.0	0.30145179182945064	0.0
32	0.0	0.0	0.0	0.35852666441582665	0.0
33	0.0	0.0	0.0	0.425247994340745	0.0
34	0.0	0.0	0.0	0.5024196530490844	0.0
35	0.0	0.0	0.0	0.649126191739417	0.0
36	0.0	0.0	0.0	0.895512789594688	0.0
37	0.0	0.0	0.0	1.3440730558369105	0.0
38	0.0	0.0	0.0	1.9136159745333525	0.0
39	0.0	0.0	0.0	2.542645380150806	0.0
40	0.0	0.0	0.0	3.175292207270213	0.0
41	0.0	0.0	0.0	4.058746925191723	0.0
42	0.0	0.0	0.0	5.003295872924002	0.0
43	0.0	0.0	0.0	5.994469364459236	0.0
44	0.0	0.0	0.0	6.940626055081271	0.0
45	0.0	0.0	0.0	7.859451116577437	0.0
46	0.0	0.0	0.0	8.828116207656072	0.0
47	0.0	0.0	0.0	9.672583160500972	0.0
48	0.0	0.0	0.0	10.45314233347803	0.0
49	0.0	0.0	0.0	11.181047926815543	0.0
50	0.0	0.0	0.0	11.925030949050628	0.0
51	0.0	0.0	0.0	12.696747536134021	0.0
52	0.0	0.0	0.0	13.576584832553579	0.0
53	0.0	0.0	0.0	14.41984597823116	0.0
54	0.0	0.0	0.0	15.202816765542854	4.019357224392675E-4
55	0.0	0.0	0.0	15.944790109165742	4.019357224392675E-4
56	0.0	0.0	0.0	16.660235695107637	4.019357224392675E-4
57	0.0	0.0	0.0	17.404218717342722	4.019357224392675E-4
58	0.0	0.0	0.0	18.00430875094455	4.019357224392675E-4
59	0.0	0.0	0.0	18.650621392626892	4.019357224392675E-4
60	0.0	0.0	0.0	19.230212704384314	4.019357224392675E-4
61	0.0	0.0	0.0	19.742278814771943	4.019357224392675E-4
62	0.0	0.0	0.0	20.35724047010402	4.019357224392675E-4
63	0.0	0.0	0.0	21.07027444171128	4.019357224392675E-4
64	0.0	0.0	0.0	21.696088361549222	4.019357224392675E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTG	15	0.0022245469	69.98653	9
ATCGTAT	75	0.0	55.989227	39
ATATCGT	70	0.0	54.989418	37
TCTCGTA	3920	0.0	52.668438	38
CGTCTTC	4005	0.0	52.62027	48
CCGTCTT	4005	0.0	52.62027	47
ATCTCGT	3935	0.0	52.556595	37
CTGCTTG	4020	0.0	52.53214	54
CGTATGC	4065	0.0	52.51142	41
TCCTCGT	20	0.006950953	52.489902	31
TAGGGCG	20	0.006950953	52.489902	25
ATCGGTA	20	0.006950953	52.489902	22
CGGAGTT	20	0.006950953	52.489902	29
AGCACGT	20	0.006950953	52.489902	30
TCGTATG	4085	0.0	52.42565	40
GCCGTCT	4040	0.0	52.424355	46
TGCCGTC	4060	0.0	52.328037	45
TATGCCG	4055	0.0	52.29573	43
AATCTCG	3905	0.0	52.243465	36
CTCGTAT	4035	0.0	52.121323	39
>>END_MODULE