##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779803_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66651
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.10446955034433	23.0	12.0	34.0	12.0	34.0
2	23.3987487059459	23.0	12.0	34.0	12.0	34.0
3	23.358464239096186	23.0	11.0	34.0	11.0	34.0
4	24.02065985506594	23.0	12.0	34.0	12.0	34.0
5	23.57477007096668	23.0	11.0	34.0	11.0	34.0
6	25.143343685766155	26.0	12.0	37.0	11.0	38.0
7	25.21671092706786	26.0	12.0	37.0	11.0	38.0
8	24.765269838412028	26.0	11.0	37.0	11.0	38.0
9	25.8650882957495	27.0	12.0	37.0	11.0	38.0
10-11	25.03542332448125	26.0	11.5	37.0	11.0	38.0
12-13	25.33710672007922	27.0	11.0	37.0	11.0	38.0
14-15	24.536901171775366	26.0	11.0	36.5	11.0	38.0
16-17	24.675413722224725	26.0	11.0	36.5	11.0	38.0
18-19	24.669494831285352	26.0	11.0	37.0	11.0	38.0
20-21	24.670275014628437	25.0	11.0	36.5	11.0	38.0
22-23	24.84018994463699	26.0	11.0	37.0	11.0	38.0
24-25	25.031507404239996	26.0	11.0	37.0	11.0	38.0
26-27	24.279620710867054	24.5	11.0	37.0	10.5	38.0
28-29	24.45006826604252	26.0	11.0	37.0	11.0	38.0
30-31	24.57071161723005	26.0	11.0	37.5	11.0	38.0
32-33	24.823948627927564	26.0	11.0	38.0	11.0	38.0
34-35	24.938087950668407	26.0	11.0	37.0	11.0	38.0
36-37	24.712059834061005	26.0	11.0	37.0	11.0	38.0
38-39	24.518536856161198	26.0	11.0	37.0	11.0	38.0
40-41	24.447000045010576	26.0	11.0	37.0	11.0	38.0
42-43	24.66746185353558	26.0	11.0	37.0	11.0	38.0
44-45	24.867488859882073	26.0	11.0	37.0	11.0	38.0
46-47	24.705143208654036	26.0	11.0	37.0	11.0	38.0
48-49	24.43006106435012	25.5	11.0	37.0	11.0	38.0
50-51	24.121708601521355	24.5	11.0	37.0	11.0	38.0
52-53	24.500622646321887	25.5	11.0	37.0	11.0	38.0
54-55	24.514065805464284	25.0	11.0	37.0	11.0	38.0
56-57	24.453946677469204	25.0	11.0	37.0	11.0	38.0
58-59	24.42414217341075	24.5	11.0	37.0	11.0	38.0
60-61	24.607957870099472	25.0	11.0	37.0	11.0	38.0
62-63	24.376565993008356	25.0	11.0	37.0	11.0	38.0
64-65	24.367248803468815	24.5	11.0	37.0	11.0	38.0
66-67	24.741249193560485	25.0	11.0	37.0	11.0	38.0
68-69	24.38396273124184	24.5	11.0	37.0	11.0	38.0
70-71	24.55677334173531	24.5	11.0	37.0	11.0	38.0
72-73	24.23524778322906	24.5	11.0	37.0	11.0	38.0
74-75	23.88665586412807	24.0	11.0	37.0	11.0	38.0
76	23.498117057508516	23.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	39.0
13	543.0
14	2247.0
15	4351.0
16	5769.0
17	6466.0
18	6292.0
19	5404.0
20	4041.0
21	2727.0
22	1878.0
23	1343.0
24	1007.0
25	762.0
26	637.0
27	555.0
28	502.0
29	513.0
30	632.0
31	725.0
32	870.0
33	1086.0
34	1589.0
35	2422.0
36	4536.0
37	9712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.42050381839732	20.37478807519767	9.462723740078918	46.741984366326086
2	11.59772546548439	35.00022505288743	28.124109165653927	25.277940315974256
3	10.789035423324481	21.951658639780348	31.331862987802133	35.927442949093034
4	10.02235525348457	32.599660920316275	27.205893384945462	30.172090441253697
5	9.963841502753148	31.706951133516377	36.78264392132151	21.546563442408967
6	15.256271756091705	39.908174288800865	23.573100468131077	21.262453486976355
7	26.069081415238728	25.756984665246225	16.844221708729705	31.329712210785342
8	14.589647411852964	40.03450862715679	22.939234808702174	22.436609152288074
9	21.112728254835176	18.787042177442345	16.713430461986288	43.38679910573619
10-11	15.258929174262374	31.993488323243234	23.648339447407707	29.09924305508668
12-13	15.246582946992543	23.155691587523066	26.70477562227123	34.89294984321315
14-15	26.346388999332337	27.369637137005725	19.82430739454318	26.45966646911876
16-17	28.682990502768153	24.054402784654393	18.114506909123644	29.148099803453814
18-19	19.816352710823036	23.720357992182986	22.611572479913576	33.851716817080394
20-21	25.04163478417428	23.344736012963047	20.016953984186284	31.59667521867639
22-23	29.43682998372081	22.273985551279456	17.819338479658512	30.469845985341227
24-25	18.984103645133946	32.25557197620423	17.472487078116444	31.287837300545384
26-27	24.01575393848462	32.058514628657164	19.55888972243061	24.366841710427607
28-29	20.196253479073047	29.56105538759312	23.584176688147522	26.658514445186317
30-31	22.066435612368906	27.04310512970548	24.19993698519152	26.69052227273409
32-33	17.278060344180883	31.430886258270696	23.523277970323026	27.7677754272254
34-35	16.467604957536835	35.52876391681421	21.314857605857814	26.688773519791138
36-37	24.22662234860706	25.404602375468006	22.04698414603951	28.321791129885426
38-39	24.861198643498096	22.451306983583926	26.197443053929952	26.49005131898803
40-41	19.192654218648023	28.572928935266802	24.377911643573565	27.85650520251161
42-43	13.997749437359339	28.006751687921984	26.09227306826707	31.903225806451612
44-45	13.955721605785751	23.840711815324138	31.140419977043056	31.06314660184706
46-47	17.361361181130714	27.843296122914417	26.060046813107668	28.735295882847193
48-49	18.62387661100359	28.930548678939548	23.31322860872305	29.132346101333813
50-51	25.41880790727334	28.1045800667692	18.312014704227465	28.164597321729996
52-53	12.88020888041896	31.825002626011013	23.45928182350205	31.835506670067975
54-55	15.988926068754408	25.948711792686403	23.50809537385772	34.55426676470147
56-57	19.348279536007443	28.962019237983768	20.166869250739055	31.52283197526974
58-59	17.637658215976533	29.05921985549545	20.979419730948436	32.323702197579586
60-61	14.148402151973768	25.97975583951738	23.10145340766697	36.77038860084188
62-63	17.292527929293307	27.298305110179093	21.613409061920873	33.79575789860672
64-65	18.19662044540489	25.995708025691815	20.8258298817456	34.981841647157694
66-67	14.380467894176082	36.08472516094179	16.68617476252645	32.84863218235567
68-69	16.495000037509097	38.41531571406066	15.505510086195903	29.584174162234344
70-71	16.90372237475807	39.56204708106405	15.284091761563968	28.250138782613917
72-73	18.20371051111428	38.71955107766866	15.813559194880606	27.263179216336454
74-75	20.797791331877892	36.35974882401926	15.579962938788983	27.26249690531386
76	16.768695234665707	37.313947905413514	18.119073340535348	27.798283519385432
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	1.5
17	1.0
18	3.0
19	9.5
20	18.0
21	22.0
22	20.0
23	37.0
24	68.5
25	81.0
26	84.0
27	132.0
28	191.5
29	206.0
30	240.0
31	307.0
32	468.5
33	597.0
34	684.5
35	904.5
36	1171.5
37	1306.0
38	1593.0
39	2098.5
40	2517.0
41	2954.5
42	3192.0
43	3471.5
44	3853.0
45	4112.0
46	4269.0
47	4363.0
48	4393.5
49	4282.5
50	4235.0
51	4120.5
52	3840.5
53	3434.5
54	3194.0
55	3021.0
56	2655.0
57	2227.0
58	1992.0
59	1856.5
60	1563.5
61	1243.5
62	1081.0
63	983.0
64	792.0
65	607.0
66	488.5
67	462.0
68	400.0
69	297.5
70	230.0
71	203.0
72	185.5
73	149.0
74	111.5
75	93.0
76	82.0
77	67.5
78	56.5
79	49.0
80	44.5
81	38.5
82	35.0
83	33.0
84	27.5
85	21.0
86	14.5
87	9.0
88	11.0
89	13.5
90	11.5
91	8.0
92	7.0
93	5.5
94	5.0
95	5.5
96	5.0
97	4.5
98	5.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.004501057748570914
7	0.0075017629142848565
8	0.0015003525828569714
9	0.0060014103314278856
10-11	0.002250528874285457
12-13	0.0
14-15	7.501762914284857E-4
16-17	0.0
18-19	0.002250528874285457
20-21	0.0
22-23	0.002250528874285457
24-25	7.501762914284857E-4
26-27	0.0015003525828569714
28-29	0.0037508814571424283
30-31	0.0
32-33	0.0
34-35	0.0075017629142848565
36-37	0.01725405470285517
38-39	0.013503173245712742
40-41	7.501762914284857E-4
42-43	0.0015003525828569714
44-45	0.006751586622856371
46-47	0.004501057748570914
48-49	0.0
50-51	0.0052512340399994
52-53	0.015003525828569713
54-55	0.012002820662855771
56-57	0.018004230994283655
58-59	0.01425334953714123
60-61	0.021755112451426084
62-63	0.01425334953714123
64-65	0.02250528874285457
66-67	0.018004230994283655
68-69	7.501762914284857E-4
70-71	0.0
72-73	0.0037508814571424283
74-75	0.006751586622856371
76	0.004501057748570914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	66651.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.68587117972723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.78472402513111	81.29810505468785
2	2.597919587379061	4.5040584537366275
3	1.381172438859754	3.5918440833595895
4	0.9363587586755975	3.246762989302486
5	0.5209685514997316	2.258030637199742
6	0.321927410561296	1.6743934824683802
7	0.20942589611783236	1.2707986376798548
8	0.10038596673416758	0.6961635984456347
9	0.057116153486681545	0.4456047171085205
>10	0.08827041902487148	0.9257175436227514
>50	0.001730792529899441	0.08852080238856132
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0030007051657139428	0.0
2	0.0	0.0	0.0	0.0030007051657139428	0.0
3	0.0	0.0	0.0	0.0030007051657139428	0.0
4	0.0	0.0	0.0	0.0075017629142848565	0.0
5	0.0	0.0	0.0	0.028506699074282455	0.0
6	0.0	0.0	0.0	0.028506699074282455	0.0
7	0.0	0.0	0.0	0.028506699074282455	0.0
8	0.0	0.0	0.0	0.028506699074282455	0.0
9	0.0	0.0	0.0	0.0315074042399964	0.0
10	0.0	0.0	0.0	0.03300775682285337	0.0
11	0.0	0.0	0.0	0.03300775682285337	0.0
12	0.0	0.0	0.0	0.03900916715428126	0.0
13	0.0	0.0	0.0	0.048011282651423084	0.0
14	0.0	0.0	0.0	0.048011282651423084	0.0
15	0.0	0.0	0.0	0.048011282651423084	0.0
16	0.0	0.0	0.0	0.048011282651423084	0.0
17	0.0	0.0	0.0	0.048011282651423084	0.0
18	0.0	0.0	0.0	0.05401269298285097	0.0
19	0.0	0.0	0.0	0.06451516106284977	0.0
20	0.0	0.0	0.0	0.06601551364570674	0.0
21	0.0	0.0	0.0	0.06601551364570674	0.0
22	0.0	0.0	0.0	0.09002115497141829	0.0
23	0.0	0.0	0.0	0.0945222127199892	0.0
24	0.0	0.0	0.0	0.0945222127199892	0.0
25	0.0	0.0	0.0	0.09752291788570314	0.0
26	0.0	0.0	0.0	0.09752291788570314	0.0
27	0.0	0.0	0.0	0.11102609113141589	0.0
28	0.0	0.0	0.0	0.12752996954284257	0.0
29	0.0	0.0	0.0	0.14403384795426924	0.0
30	0.0	0.0	0.0	0.1620380789485529	0.0
31	0.0	0.0	0.0	0.18004230994283657	0.0
32	0.0	0.0	0.0	0.23255465034283057	0.0
33	0.0	0.0	0.0	0.2415567658399724	0.0
34	0.0	0.0	0.0	0.2835666381599676	0.0
35	0.0	0.0	0.0	0.315074042399964	0.0
36	0.0	0.0	0.0	0.45310648002280535	0.0
37	0.0	0.0	0.0	0.7051657139427766	0.0
38	0.0	0.0	0.0	0.9557245952798907	0.0
39	0.0	0.0	0.0	1.14927007846844	0.0
40	0.0	0.0	0.0	1.3758233184798427	0.0
41	0.0	0.0	0.0	1.620380789485529	0.0
42	0.0	0.0	0.0	1.9204513060569233	0.0
43	0.0	0.0	0.0	2.148504898651183	0.0
44	0.0	0.0	0.0	2.465079293634004	0.0
45	0.0	0.0	0.0	2.766650162788255	0.0
46	0.0	0.0	0.0	3.0772231474396485	0.0
47	0.0	0.0	0.0	3.414802478582467	0.0
48	0.0	0.0	0.0	3.6503578340910114	0.0
49	0.0	0.0	0.0	3.891914599930984	0.0
50	0.0	0.0	0.0	4.148474891599526	0.0
51	0.0	0.0	0.0	4.4905552804909155	0.0
52	0.0	0.0	0.0	4.736613104079459	0.0
53	0.0	0.0	0.0	5.051687146479423	0.0
54	0.0	0.0	0.0	5.299245322650823	0.0
55	0.0	0.0	0.0	5.630823243462213	0.0
56	0.0	0.0	0.0	5.912889529039324	0.0
57	0.0	0.0	0.0	6.182952993953579	0.0
58	0.0	0.0	0.0	6.436512580456407	0.0
59	0.0	0.0	0.0	6.661565467884953	0.0
60	0.0	0.0	0.0	6.955634574124919	0.0
61	0.0	0.0	0.0	7.18818922446775	0.0
62	0.0	0.0	0.0	7.459753041964862	0.0
63	0.0	0.0	0.0	7.768825674033398	0.0
64	0.0	0.0	0.0	7.984876445964802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACA	15	0.002215122	70.00451	29
AGGACGT	15	0.002215122	70.00451	21
GCTGCGG	15	0.002215122	70.00451	17
GGAAGCC	15	0.002215122	70.00451	7
AGATTTC	15	0.002215122	70.00451	40
GGCGGTC	15	0.002215122	70.00451	47
CGTGTAA	15	0.002215122	70.00451	35
CCGTTTG	15	0.002215122	70.00451	19
ACGACGT	15	0.002215122	70.00451	12
TACGTTT	15	0.002215122	70.00451	33
AGTGTCG	15	0.002215122	70.00451	35
ACGTCGA	20	7.87148E-5	70.0045	15
TCGGCGG	25	2.8232007E-6	69.95199	45
GTCCATA	40	1.0462827E-8	61.25394	22
GTTTAGA	30	8.29093E-6	58.33709	36
GACGTCG	30	8.29093E-6	58.33709	14
GTGGTCC	30	8.29093E-6	58.33709	48
GCCGTAC	30	8.29093E-6	58.33709	54
CTCGGCG	25	2.3739075E-4	56.0036	44
TCTCGGC	25	2.3739075E-4	56.0036	43
>>END_MODULE