FastQCFastQC Report
Wed 25 May 2016
SRR1779803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66651
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2932043.9903377293664TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG36545.482288337759374TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC20033.0052062234625136RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT7261.089255975154161TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6120.9182157807084664RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT4140.6211459693027862No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT3920.5881382124799328No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT2970.4456047171085205No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2720.40809590253709627TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2030.3045715743199652RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT1790.2685631123313979No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1760.26406205458282694TruSeq Adapter, Index 5 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1430.21455041934854693No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG1380.20704865643426207No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1320.1980465409371202TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT1070.16053772636569594No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1030.15453631603426804No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT1020.15303596345141107RNA PCR Primer, Index 5 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC990.14853490570284017TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT940.1410331427885553No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT790.11852785404570074Illumina PCR Primer Index 5 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTTG207.8581164E-570.0285322
CATCACT150.002212110770.0285219
AGTCTTC150.002212110770.0285248
GACCACG150.002212110770.0285218
ACAATAA150.002212110770.0285270
TTCAAGA150.002218711769.9759943
TATGCAG150.002218711769.9759943
ATGCCGC150.002218711769.9759944
ATGCAGT150.002218711769.9759944
AGCCGCC150.002218711769.9759936
TCCGAGA150.002218711769.9759913
TTTACAC252.8174527E-669.975992
CTATACA401.0497388E-861.2289961
TTGCAAA352.925044E-760.02445258
GCAAAAA352.925044E-760.02445260
TCTTTAT1000.059.479591
TGCTTGC308.274092E-658.357155
GCTTGCA308.274092E-658.357156
CTTGCAA308.274092E-658.357157
ACCACGA252.3698868E-456.02282319
Produced by FastQC (version 0.11.5)