##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779803_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66651
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.61814526413707	34.0	34.0	34.0	34.0	34.0
2	33.700649652668375	34.0	34.0	34.0	34.0	34.0
3	33.80018304301511	34.0	34.0	34.0	34.0	34.0
4	33.59502483083524	34.0	34.0	34.0	34.0	34.0
5	33.665721444539464	34.0	34.0	34.0	34.0	34.0
6	37.41598775712293	38.0	38.0	38.0	37.0	38.0
7	37.44877046105835	38.0	38.0	38.0	37.0	38.0
8	37.342725540502016	38.0	38.0	38.0	37.0	38.0
9	37.28880286867414	38.0	38.0	38.0	37.0	38.0
10-11	37.22505288742855	38.0	38.0	38.0	36.5	38.0
12-13	37.378786514830985	38.0	38.0	38.0	36.5	38.0
14-15	37.40202697633944	38.0	38.0	38.0	37.0	38.0
16-17	37.16880466909724	38.0	38.0	38.0	36.0	38.0
18-19	36.95629472926137	38.0	38.0	38.0	35.0	38.0
20-21	37.121333513375646	38.0	38.0	38.0	35.5	38.0
22-23	37.160582736943184	38.0	38.0	38.0	36.0	38.0
24-25	37.158714797977524	38.0	38.0	38.0	36.0	38.0
26-27	37.02898681190079	38.0	38.0	38.0	35.5	38.0
28-29	36.61865538401524	38.0	37.0	38.0	34.0	38.0
30-31	36.589608558011136	38.0	37.0	38.0	34.5	38.0
32-33	36.42537996429161	38.0	37.0	38.0	34.0	38.0
34-35	36.461178376918575	38.0	37.0	38.0	34.0	38.0
36-37	36.65512145354158	38.0	37.0	38.0	34.5	38.0
38-39	33.92332448125309	37.0	34.0	38.0	25.0	38.0
40-41	35.968305051687146	37.5	36.5	38.0	32.5	38.0
42-43	37.057088415777706	38.0	37.5	38.0	35.5	38.0
44-45	36.87530569683876	38.0	37.5	38.0	35.0	38.0
46-47	37.0374412987052	38.0	38.0	38.0	36.0	38.0
48-49	36.63855005926392	38.0	37.0	38.0	34.0	38.0
50-51	36.653553585092496	38.0	37.0	38.0	34.0	38.0
52-53	36.56249718683891	38.0	37.0	38.0	34.0	38.0
54-55	36.61232389611558	38.0	37.0	38.0	34.0	38.0
56-57	36.449940736072975	38.0	37.0	38.0	34.0	38.0
58-59	36.41246192855321	38.0	37.0	38.0	34.0	38.0
60-61	35.944824533765434	38.0	37.0	38.0	32.5	38.0
62-63	35.93782538896641	38.0	37.0	38.0	33.0	38.0
64-65	36.4996324136172	38.0	37.0	38.0	34.0	38.0
66-67	36.034688151715656	38.0	37.0	38.0	32.5	38.0
68-69	35.839169704880646	38.0	37.0	38.0	31.0	38.0
70-71	32.25926092631769	36.0	29.5	38.0	19.5	38.0
72-73	25.852860422199218	24.0	16.0	38.0	10.0	38.0
74-75	24.823663560936822	23.5	11.0	38.0	11.0	38.0
76	24.934809680274864	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	10.0
24	24.0
25	52.0
26	91.0
27	230.0
28	373.0
29	594.0
30	999.0
31	1613.0
32	2473.0
33	3856.0
34	7320.0
35	17070.0
36	15735.0
37	16209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.760053438208317	8.560620843903392	12.442396313364055	64.23692940452423
2	7.257205443279171	7.923361990067666	68.3320580336379	16.48737453301526
3	6.9871419783649165	9.884322815861728	69.7828989812606	13.345636224512761
4	4.903152240776582	56.62780753477067	26.283176546488423	12.185863677964322
5	4.441043645256635	22.269733387346026	67.6659014868494	5.623321480547929
6	9.97734467599886	68.24353723124933	14.1183178046841	7.660800288067696
7	9.659269928433181	20.356783844203388	13.495671482798457	56.48827474456497
8	8.622526293679014	67.4048401374323	8.651032992753297	15.321600576135392
9	8.562512190364737	14.78747505663831	8.415477637244754	68.23453511575221
10-11	8.965356858861833	40.15693688016684	35.79316139292734	15.08454486804399
12-13	9.266177551724656	10.960075617770176	40.908613524178186	38.86513330632699
14-15	9.5017329072332	9.083134536616106	14.966767190289717	66.44836536586098
16-17	37.45480187844144	35.16376348441884	8.994613734227544	18.386820902912184
18-19	39.096187604086964	16.38910143883813	9.285682135301796	35.22902882177312
20-21	11.175376213410152	10.21965161813026	9.222667326821803	69.38230484163779
22-23	9.043375193170396	38.850879956789846	9.297684955964652	42.80805989407511
24-25	37.35333833458365	38.11927981995499	9.319579894973744	15.207801950487623
26-27	38.819372552549844	41.93935574860092	9.612008822073188	9.629262876776043
28-29	10.78528454186734	42.20266762689232	9.677274159427466	37.334773671812876
30-31	8.66453616599901	42.42171910398944	9.716283326581747	39.197461403429806
32-33	8.831075302696135	69.66362095092347	10.381689697078814	11.123614049301587
34-35	37.412792006121435	18.91869589353498	35.43082624416738	8.237685856176201
36-37	38.98591168924697	34.983721174476	16.752936940180945	9.277430196096082
38-39	11.566218061244392	16.23456512280386	37.745870279515685	34.453346536436065
40-41	8.260941321210485	10.395943046615955	41.53651108010382	39.806604552069736
42-43	37.00900225056264	9.798199549887473	38.5311327831958	14.661665416354088
44-45	10.564732712187364	37.82163808494997	41.75931343865808	9.854315764204587
46-47	36.967937465304345	11.462693733027262	17.045505693838052	34.52386310783034
48-49	35.35880932019025	9.388456287227498	11.503203252764399	43.74953113981786
50-51	13.55052183764884	8.938392394900923	40.08433436625425	37.42675140119599
52-53	7.8045156788150285	9.283479278751997	67.58886162573442	15.323143416698557
54-55	7.534920027320559	9.102099329745633	45.1689896647227	38.193990978211104
56-57	36.31723920532585	8.99675015198481	17.639994896312587	37.04601574637676
58-59	10.454784240150094	10.504315196998123	64.00675422138836	15.034146341463414
60-61	36.17030856045144	36.28136631048145	18.225477248169046	9.322847880898067
62-63	9.31234241805739	70.80681954616401	10.280345779805499	9.600492255973107
64-65	6.974039615846339	74.34273709483794	9.253451380552221	9.429771908763506
66-67	7.251673520847716	73.81877945546783	9.010746555398793	9.918800468285655
68-69	6.986811109277237	73.95306615451558	9.0851801281378	9.97494260806938
70-71	7.448005762131989	69.16584136130368	9.884157138141113	13.501995738423217
72-73	9.287632996683524	57.24897579422843	11.865029938322554	21.59836127076549
74-75	10.642687794483958	51.95747426547823	13.345187719456197	24.054650220581618
76	10.584598763579617	53.428665746353765	12.847368105155754	23.13936738491087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	3.5
17	7.0
18	8.5
19	10.0
20	13.0
21	16.0
22	20.0
23	31.0
24	54.0
25	70.0
26	73.0
27	100.0
28	145.0
29	166.0
30	188.0
31	271.0
32	346.0
33	360.0
34	448.0
35	577.0
36	664.0
37	710.0
38	959.5
39	6025.0
40	12794.5
41	13226.5
42	11705.0
43	9467.5
44	5577.0
45	3215.0
46	2506.0
47	2197.5
48	1726.5
49	1451.5
50	1339.0
51	1258.5
52	1042.0
53	819.5
54	733.0
55	698.0
56	602.5
57	476.5
58	411.0
59	379.0
60	318.0
61	252.0
62	215.0
63	227.5
64	197.5
65	135.0
66	108.0
67	101.0
68	100.5
69	89.0
70	66.5
71	55.0
72	49.5
73	42.0
74	40.5
75	41.0
76	38.0
77	33.5
78	26.5
79	21.0
80	16.0
81	11.0
82	6.5
83	2.0
84	6.5
85	8.0
86	3.0
87	1.0
88	2.5
89	2.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048011282651423084
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015003525828569714
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0015003525828569714
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01725405470285517
52-53	0.024755817617140026
54-55	0.05176216410856552
56-57	0.04876145894285157
58-59	0.03900916715428126
60-61	0.02850669907428246
62-63	0.02850669907428246
64-65	0.016503878411426684
66-67	0.037508814571424284
68-69	0.0060014103314278856
70-71	0.013503173245712742
72-73	0.0210049361599976
74-75	0.013503173245712742
76	0.0105024680799988
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	66651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.90223702570104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.10897254457968	8.76355943646757
2	13.539013114444758	4.306011912799508
3	8.774412680441552	4.18598370617095
4	6.670440607604491	4.242997104319516
5	5.075950561373714	4.035948447885253
6	3.5003302198320596	3.3397848494396185
7	2.311538824417398	2.573104679599706
8	1.509576375129729	1.9204513060569233
9	0.8680064156995944	1.2422919386055724
>10	2.368147938484763	5.79586202757648
>50	0.10378337579016889	1.1207633793941576
>100	0.12265308047929051	3.98793716523383
>500	0.018869704689121615	2.0074717558626274
>1k	0.018869704689121615	8.487494561221887
>5k	0.0	0.0
>10k+	0.009434852344560807	43.9903377293664
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	29320	43.9903377293664	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3654	5.482288337759374	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2003	3.0052062234625136	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	726	1.089255975154161	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	612	0.9182157807084664	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	414	0.6211459693027862	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT	392	0.5881382124799328	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	297	0.4456047171085205	No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	272	0.40809590253709627	TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	203	0.3045715743199652	RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT	179	0.2685631123313979	No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	176	0.26406205458282694	TruSeq Adapter, Index 5 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	143	0.21455041934854693	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG	138	0.20704865643426207	No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	132	0.1980465409371202	TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT	107	0.16053772636569594	No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	103	0.15453631603426804	No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT	102	0.15303596345141107	RNA PCR Primer, Index 5 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	99	0.14853490570284017	TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT	94	0.1410331427885553	No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT	79	0.11852785404570074	Illumina PCR Primer Index 5 (96% over 26bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015003525828569714	0.0
2	0.0	0.0	0.0	0.0015003525828569714	0.0
3	0.0	0.0	0.0	0.0015003525828569714	0.0
4	0.0	0.0	0.0	0.0075017629142848565	0.0
5	0.0	0.0	0.0	0.0315074042399964	0.0
6	0.0	0.0	0.0	0.0315074042399964	0.0
7	0.0	0.0	0.0	0.0315074042399964	0.0
8	0.0	0.0	0.0	0.0315074042399964	0.0
9	0.0	0.0	0.0	0.03300775682285337	0.0
10	0.0	0.0	0.0	0.03900916715428126	0.0
11	0.0	0.0	0.0	0.03900916715428126	0.0
12	0.0	0.0	0.0	0.04651093006856611	0.0
13	0.0	0.0	0.0	0.05701339814856491	0.0
14	0.0	0.0	0.0	0.05701339814856491	0.0
15	0.0	0.0	0.0	0.05701339814856491	0.0
16	0.0	0.0	0.0	0.05701339814856491	0.0
17	0.0	0.0	0.0	0.05701339814856491	0.0
18	0.0	0.0	0.0	0.06601551364570674	0.0
19	0.0	0.0	0.0	0.07951868689141948	0.0
20	0.0	0.0	0.0	0.07951868689141948	0.0
21	0.0	0.0	0.0	0.08251939205713342	0.0
22	0.0	0.0	0.0	0.08552009722284737	0.0
23	0.0	0.0	0.0	0.09152150755427525	0.0
24	0.0	0.0	0.0	0.09152150755427525	0.0
25	0.0	0.0	0.0	0.09752291788570314	0.0
26	0.0	0.0	0.0	0.09752291788570314	0.0
27	0.0	0.0	0.0	0.11252644371427285	0.0
28	0.0	0.0	0.0	0.12752996954284257	0.0
29	0.0	0.0	0.0	0.14403384795426924	0.0
30	0.0	0.0	0.0	0.16053772636569594	0.0
31	0.0	0.0	0.0	0.18004230994283657	0.0
32	0.0	0.0	0.0	0.22955394517711664	0.0
33	0.0	0.0	0.0	0.24305711842282937	0.0
34	0.0	0.0	0.0	0.2790655804113967	0.0
35	0.0	0.0	0.0	0.316574394982821	0.0
36	0.0	0.0	0.0	0.4546068326056623	0.0
37	0.0	0.0	0.0	0.7111671242742045	0.0
38	0.0	0.0	0.0	0.9707281211084605	0.0
39	0.0	0.0	0.0	1.161272899131296	0.0
40	0.0	0.0	0.0	1.3998289598055542	0.0
41	0.0	0.0	0.0	1.6353843153140988	0.0
42	0.0	0.0	0.0	1.9219516586397805	0.0
43	0.0	0.0	0.0	2.1455041934854693	0.0
44	0.0	0.0	0.0	2.454576825554005	0.0
45	0.0	0.0	0.0	2.7426445214625437	0.0
46	0.0	0.0	0.0	3.05171715353108	0.0
47	0.0	0.0	0.0	3.395297895005326	0.0
48	0.0	0.0	0.0	3.629352897931014	0.0
49	0.0	0.0	0.0	3.884412837016699	0.0
50	0.0	0.0	0.0	4.140973128685241	0.0
51	0.0	0.0	0.0	4.472551049496632	0.0
52	0.0	0.0	0.0	4.721609578250889	0.0
53	0.0	0.0	0.0	5.027681505153711	0.0
54	0.0	0.0	0.0	5.261736508079399	0.0
55	0.0	0.0	0.0	5.572309492730792	0.0
56	0.0	0.0	0.0	5.846874015393618	0.0
57	0.0	0.0	0.0	6.1154371277250155	0.0
58	0.0	0.0	0.0	6.362995303896415	0.0
59	0.0	0.0	0.0	6.59855065940496	0.0
60	0.0	0.0	0.0	6.879116592399214	0.0
61	0.0	0.0	0.0	7.122173710822043	0.0
62	0.0	0.0	0.0	7.3877361179877274	0.0
63	0.0	0.0	0.0	7.695308397473406	0.0
64	0.0	0.0	0.0	7.909858816821953	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTTTG	20	7.8581164E-5	70.02853	22
CATCACT	15	0.0022121107	70.02852	19
AGTCTTC	15	0.0022121107	70.02852	48
GACCACG	15	0.0022121107	70.02852	18
ACAATAA	15	0.0022121107	70.02852	70
TTCAAGA	15	0.0022187117	69.97599	43
TATGCAG	15	0.0022187117	69.97599	43
ATGCCGC	15	0.0022187117	69.97599	44
ATGCAGT	15	0.0022187117	69.97599	44
AGCCGCC	15	0.0022187117	69.97599	36
TCCGAGA	15	0.0022187117	69.97599	13
TTTACAC	25	2.8174527E-6	69.97599	2
CTATACA	40	1.0497388E-8	61.228996	1
TTGCAAA	35	2.925044E-7	60.024452	58
GCAAAAA	35	2.925044E-7	60.024452	60
TCTTTAT	100	0.0	59.47959	1
TGCTTGC	30	8.274092E-6	58.3571	55
GCTTGCA	30	8.274092E-6	58.3571	56
CTTGCAA	30	8.274092E-6	58.3571	57
ACCACGA	25	2.3698868E-4	56.022823	19
>>END_MODULE