FastQCFastQC Report
Wed 25 May 2016
SRR1779802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83413
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2609231.280495845971252No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG34674.15642645630777No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC22702.721398343183916No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT6050.7253066068838191RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4350.5215014446189442No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3150.3776389771378562No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT2440.29252035054487907No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2300.2757363960054188No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2000.2397707791351468No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1730.20740172395190196No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1570.18822006162109023RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1170.14026590579406087No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT1030.1234819512546006No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1000.1198853895675734No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG920.11029455840216754No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC860.10310143502811311No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG840.10070372723676166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG150.002219691669.9838143
CTCGTCT150.002219691669.9838139
GGCCGTG150.002219691669.9838127
CGAGCAC252.8218146E-669.9838115
CGAGACA150.002219691669.9838123
CGAGAAT207.894686E-569.9838123
GCTTGCA352.9348666E-760.02211456
CGTCAAA553.45608E-1157.25948332
AAACTCG509.658834E-1055.9870536
AATTTCG252.3808758E-455.9870527
ATATCGT509.658834E-1055.9870537
CTTGCAA452.6553607E-854.46450857
GTCAATC37900.053.826633
CGTCAAT38250.053.7914832
TCAATCT37950.053.75568434
CAATCTC38150.053.56559435
ATCTCGT38550.053.5544137
AATCTCG38300.053.53853236
TCGTCAA39850.053.387931
TCTCGTA38450.053.32966638