FastQCFastQC Report
Wed 25 May 2016
SRR1779801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150335
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT207.892839E-570.0239757
CGAATGG150.002221048370.0006632
GTATCGG150.002223982569.977381
CGATACA150.002223982569.9773825
ACGATAC452.6893758E-854.42685324
TTTGCCG200.006930945452.5179855
CTACGTG200.00694007852.50050432
TTGGTAA200.00694007852.50050433
GGCTCGT200.00694007852.50050433
GACATAG200.0069492252.4830361
TCGCCGT552.2646418E-950.90957352
TACGTGT352.0786223E-550.00047733
AGATCTC751.4551915E-1146.6671140
TGGTCGC604.8912625E-946.66710749
TCTCGGT703.8016879E-1045.00042743
TAGATCT802.910383E-1143.7504239
CCGTATC503.452511E-642.0143855
ACGTGTA503.460429E-642.000434
CGTGTAG503.460429E-642.000435
GCCGTAT602.5727968E-740.84731354