##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779801_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150335
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.616742608175077	34.0	23.0	34.0	12.0	34.0
2	29.196567665546944	34.0	27.0	34.0	12.0	34.0
3	28.902258289819404	34.0	23.0	34.0	11.0	34.0
4	29.275604483320585	34.0	27.0	34.0	12.0	34.0
5	29.520045232314498	34.0	31.0	34.0	11.0	34.0
6	32.013217148368646	37.0	28.0	38.0	12.0	38.0
7	32.17508896797153	37.0	31.0	38.0	12.0	38.0
8	31.565151162403964	37.0	27.0	38.0	11.0	38.0
9	32.573918249243356	37.0	31.0	38.0	12.0	38.0
10-11	31.170106761565837	37.0	26.0	38.0	11.0	38.0
12-13	31.99500116406692	37.0	29.5	38.0	11.0	38.0
14-15	30.801629693684106	36.5	25.5	38.0	11.0	38.0
16-17	31.412112947750025	37.0	28.0	38.0	11.0	38.0
18-19	32.208753783217475	37.0	31.0	38.0	11.0	38.0
20-21	31.030551767718762	36.5	27.0	38.0	11.0	38.0
22-23	31.85331759071407	37.0	29.0	38.0	11.0	38.0
24-25	32.134925333422025	37.0	31.0	38.0	11.0	38.0
26-27	31.052256626866665	37.0	25.5	38.0	11.0	38.0
28-29	32.19864635647055	37.5	31.0	38.0	11.0	38.0
30-31	32.58934712475471	38.0	32.0	38.0	11.0	38.0
32-33	32.71367944923006	38.0	33.5	38.0	11.0	38.0
34-35	32.66366780856088	38.0	33.0	38.0	11.0	38.0
36-37	32.19604882429241	37.5	29.0	38.0	11.0	38.0
38-39	32.096893604283764	37.5	29.0	38.0	11.0	38.0
40-41	32.102038780057875	37.0	29.5	38.0	11.0	38.0
42-43	32.45753483885988	37.5	32.5	38.0	11.0	38.0
44-45	32.52092327136063	38.0	31.5	38.0	11.0	38.0
46-47	32.422187115442185	38.0	31.5	38.0	11.0	38.0
48-49	32.1352579239698	37.0	29.5	38.0	11.0	38.0
50-51	31.54776665447168	37.0	28.5	38.0	11.0	38.0
52-53	32.07014002062061	37.0	30.5	38.0	11.0	38.0
54-55	32.19442910832474	37.0	31.0	38.0	11.0	38.0
56-57	32.226208135164796	37.0	31.0	38.0	11.0	38.0
58-59	32.450144676888286	38.0	31.5	38.0	11.0	38.0
60-61	32.321112847972856	37.5	31.0	38.0	11.0	38.0
62-63	32.317936608241595	38.0	31.0	38.0	11.0	38.0
64-65	32.3358865201051	37.5	31.0	38.0	11.0	38.0
66-67	32.23956164565803	37.0	31.0	38.0	11.0	38.0
68-69	31.62191771709848	37.0	27.0	38.0	11.0	38.0
70-71	31.737689160874048	37.0	28.0	38.0	11.0	38.0
72-73	31.913483220806867	37.0	30.0	38.0	11.0	38.0
74-75	31.951255529317855	37.0	30.0	38.0	11.0	38.0
76	30.9484218578508	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	29.0
13	434.0
14	1617.0
15	2967.0
16	3919.0
17	4364.0
18	4382.0
19	3863.0
20	2970.0
21	2260.0
22	1779.0
23	1516.0
24	1435.0
25	1457.0
26	1514.0
27	1849.0
28	2173.0
29	2650.0
30	3046.0
31	3812.0
32	4895.0
33	6577.0
34	8912.0
35	13397.0
36	23481.0
37	45036.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.27876594782285	22.593691380526028	8.771801455425917	30.35574121622521
2	19.90354874114478	21.69288588818306	28.191705191738453	30.211860178933712
3	21.937007350251108	19.668074633318923	29.281271826254695	29.113646190175274
4	14.055941730136029	21.68357335284531	30.68214321348987	33.578341703528785
5	18.60777596700702	30.809192802740547	31.669937140386473	18.913094089865968
6	28.481816790947974	35.10054479781283	15.298907078380374	21.118731332858825
7	27.059981773310536	27.289478410685895	20.53895123362758	25.111588582375987
8	23.770562684174465	34.04774733425129	16.458129618912682	25.72356036266156
9	25.15782681292949	17.17634693293774	17.306732835294667	40.35909341883811
10-11	23.71384668600165	25.152994705052816	23.193332091637174	27.939826517308358
12-13	23.793939514881814	21.591517582457787	24.742823503587	29.8717193990734
14-15	25.48683103346205	23.546158732692763	21.721560919150296	29.2454493146949
16-17	26.682254180690997	24.271621855335454	21.575957534556387	27.470166429417166
18-19	23.65902899963081	23.86956651888019	23.764131457897484	28.70727302359151
20-21	25.0084810589683	23.748295473442646	23.508165097947916	27.735058369641134
22-23	27.05152211582914	23.32647081322526	22.51726993650783	27.104737134437766
24-25	24.048999358072486	26.60140957968715	21.594042380519994	27.755548681720366
26-27	24.714302819056236	26.773384596964096	22.709433661048067	25.802878922931605
28-29	23.623482454681525	25.90587061367038	24.45834026276401	26.012306668884083
30-31	24.443572312317905	25.43370095919752	24.030824697007997	26.09190203147658
32-33	22.65939402002195	26.583962483786213	24.389862640103768	26.366780856088067
34-35	22.670715523463304	27.608169998769377	23.443346493226592	26.277767984540727
36-37	24.8898099575207	24.675583882481696	23.830322303795196	26.60428385620241
38-39	24.831944652330822	24.00405794209117	25.270002827254736	25.89399457832327
40-41	22.889955399459204	24.859230582467763	25.255679833970245	26.99513418410278
42-43	21.150098114211595	24.881098879169855	25.572887218545247	28.3959157880733
44-45	20.922652209342935	23.16475694733166	28.059071028254976	27.85351981507043
46-47	21.106705958264875	24.482967358260883	27.155771674128076	27.254555009346166
48-49	21.201317058569195	25.104267136727977	26.423653839757876	27.270761964944956
50-51	23.167158351265684	25.30939544412922	24.67181300242461	26.851633202180487
52-53	18.897999234952685	26.758694097493642	26.099422888219937	28.243883779333746
54-55	19.747739836749357	25.17645571809661	25.927182496124964	29.14862194902908
56-57	20.375877324285664	25.710341615939857	25.281907993214247	28.631873066560225
58-59	20.323851087043295	25.573125948002872	25.32331887490354	28.779704090050295
60-61	19.687629946275013	24.879493022404816	25.619999667337535	29.812877363982636
62-63	21.138219494752946	24.91443396697101	24.495667647890368	29.45167889038567
64-65	21.382948996021344	24.498675998988702	23.812391052680603	30.30598395230935
66-67	20.238043756673775	27.210703320171515	22.538312869864313	30.0129400532904
68-69	21.008081950310974	28.319752552632455	21.986563341869825	28.68560215518675
70-71	20.803482934551962	28.95605090033459	22.467788176913917	27.77267798819953
72-73	21.519989356748486	29.313842878999534	21.894831370983837	27.271336393268143
74-75	22.142282182795483	29.846129283333667	21.628714550861158	26.38287398300969
76	21.48221567362252	30.880269276054523	21.390417018672377	26.24709803165058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.5
11	0.5
12	0.0
13	0.5
14	2.0
15	5.5
16	10.5
17	13.0
18	22.0
19	47.0
20	93.5
21	124.0
22	133.0
23	177.0
24	249.0
25	286.0
26	362.5
27	492.0
28	678.5
29	812.0
30	917.0
31	1203.5
32	1583.0
33	1781.0
34	1994.0
35	2403.5
36	2887.0
37	3174.0
38	3504.5
39	4414.0
40	5287.0
41	6003.5
42	6426.0
43	6640.0
44	7159.0
45	7756.0
46	8048.0
47	8229.0
48	8221.0
49	7937.0
50	7842.0
51	7625.0
52	7147.0
53	6640.0
54	6394.0
55	6046.5
56	5472.5
57	4946.0
58	4646.0
59	4386.0
60	3830.0
61	3312.5
62	3091.0
63	2878.0
64	2513.5
65	2315.0
66	2079.0
67	1890.0
68	1778.0
69	1534.0
70	1411.0
71	1420.0
72	1341.5
73	1107.5
74	977.5
75	1003.0
76	890.5
77	727.5
78	601.0
79	525.0
80	514.0
81	460.0
82	376.5
83	336.0
84	310.0
85	219.5
86	150.5
87	146.0
88	108.0
89	64.5
90	51.5
91	36.0
92	28.0
93	21.0
94	12.0
95	11.5
96	13.0
97	10.0
98	6.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.651810955532643E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0026607243822130574
7	0.003991086573319586
8	0.0013303621911065287
9	0.007982173146639172
10-11	0.001995543286659793
12-13	3.3259054777663217E-4
14-15	3.3259054777663217E-4
16-17	6.651810955532643E-4
18-19	0.0036584960255429543
20-21	0.0
22-23	9.977716433298965E-4
24-25	0.004323677121096218
26-27	6.651810955532643E-4
28-29	0.0066518109555326445
30-31	6.651810955532643E-4
32-33	0.0
34-35	0.00299331492998969
36-37	0.01762729903216151
38-39	0.008314763694415804
40-41	3.3259054777663217E-4
42-43	0.0
44-45	0.004988858216649483
46-47	0.003991086573319586
48-49	0.0
50-51	9.977716433298965E-4
52-53	0.011640669172182127
54-55	0.009312535337745701
56-57	0.013303621911065289
58-59	0.012638440815512025
60-61	0.021618385605481093
62-63	0.008314763694415804
64-65	0.021950976153257723
66-67	0.01762729903216151
68-69	0.0
70-71	0.0013303621911065287
72-73	0.0033259054777663223
74-75	0.009312535337745701
76	0.003991086573319586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	150335.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.45087272868908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33864265660416	55.10137973610757
2	9.90970836396787	13.764757887287162
3	5.280212161516667	11.00146028429911
4	2.6533274208596183	7.371036200546488
5	1.3161193438564538	4.570281852297016
6	0.6345734060900782	2.6443006118023646
7	0.34077083007621245	1.6566782084490934
8	0.17288373140772917	0.9605540821487251
9	0.09993279255835442	0.6246377691653483
>10	0.2538292930628464	2.304913367897122
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0013303621911065287	0.0
2	0.0	0.0	0.0	0.0026607243822130574	0.0
3	0.0	0.0	0.0	0.003325905477766322	0.0
4	0.0	0.0	0.0	0.006651810955532644	0.0
5	0.0	0.0	0.0	0.007982173146639172	0.0
6	0.0	0.0	0.0	0.007982173146639172	0.0
7	0.0	0.0	0.0	0.008647354242192437	0.0
8	0.0	0.0	0.0	0.008647354242192437	0.0
9	0.0	0.0	0.0	0.012638440815512023	0.0
10	0.0	0.0	0.0	0.015299165197725081	0.0
11	0.0	0.0	0.0	0.015964346293278343	0.0
12	0.0	0.0	0.0	0.018625070675491403	0.0
13	0.0	0.0	0.0	0.020620613962151195	0.0
14	0.0	0.0	0.0	0.020620613962151195	0.0
15	0.0	0.0	0.0	0.024611700535470783	0.0
16	0.0	0.0	0.0	0.025276881631024047	0.0
17	0.0	0.0	0.0	0.026607243822130575	0.0
18	0.0	0.0	0.0	0.02727242491768384	0.0
19	0.0	0.0	0.0	0.03192869258655669	0.0
20	0.0	0.0	0.0	0.03458941696876975	0.0
21	0.0	0.0	0.0	0.0392456846376426	0.0
22	0.0	0.0	0.0	0.049223401070941565	0.0
23	0.0	0.0	0.0	0.05055376326204809	0.0
24	0.0	0.0	0.0	0.06319220407756011	0.0
25	0.0	0.0	0.0	0.0691788339375395	0.0
26	0.0	0.0	0.0	0.07716100708417867	0.0
27	0.0	0.0	0.0	0.1436791166395051	0.0
28	0.0	0.0	0.0	0.20088469085708585	0.0
29	0.0	0.0	0.0	0.2487777297369209	0.0
30	0.0	0.0	0.0	0.2953404064256494	0.0
31	0.0	0.0	0.0	0.35520670502544316	0.0
32	0.0	0.0	0.0	0.41773372800745	0.0
33	0.0	0.0	0.0	0.4709482156517112	0.0
34	0.0	0.0	0.0	0.5673994745069345	0.0
35	0.0	0.0	0.0	0.7177304021019723	0.0
36	0.0	0.0	0.0	0.9731599427944257	0.0
37	0.0	0.0	0.0	1.4421126151594772	0.0
38	0.0	0.0	0.0	1.9742574916020887	0.0
39	0.0	0.0	0.0	2.6307912329131606	0.0
40	0.0	0.0	0.0	3.3259054777663217	0.0
41	0.0	0.0	0.0	4.269132271260851	0.0
42	0.0	0.0	0.0	5.254930654870789	0.0
43	0.0	0.0	0.0	6.323211494329331	0.0
44	0.0	0.0	0.0	7.14404496624206	0.0
45	0.0	0.0	0.0	7.890378155452822	0.0
46	0.0	0.0	0.0	8.677952572587888	0.0
47	0.0	0.0	0.0	9.421625037416437	0.0
48	0.0	0.0	0.0	10.16197159676722	0.0
49	0.0	0.0	0.0	10.821831243556058	0.0
50	0.0	0.0	0.0	11.49033824458709	0.0
51	0.0	0.0	0.0	12.257957228855556	0.0
52	0.0	0.0	0.0	13.171250873050187	0.0
53	0.0	0.0	0.0	13.986097715102936	0.0
54	0.0	0.0	0.0	14.745069345129211	0.0
55	0.0	0.0	0.0	15.42355406259354	0.0
56	0.0	0.0	0.0	16.10203878005787	0.0
57	0.0	0.0	0.0	16.67675524661589	0.0
58	0.0	0.0	0.0	17.31067283067815	0.0
59	0.0	0.0	0.0	17.826853360827485	0.0
60	0.0	0.0	0.0	18.339042804403498	0.0
61	0.0	0.0	0.0	18.906442278910433	0.0
62	0.0	0.0	0.0	19.411979911530914	0.0
63	0.0	0.0	0.0	19.943459606877973	0.0
64	0.0	0.0	0.0	20.53746632520704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAT	20	7.892839E-5	70.02397	57
CGAATGG	15	0.0022210483	70.00066	32
GTATCGG	15	0.0022239825	69.97738	1
CGATACA	15	0.0022239825	69.97738	25
ACGATAC	45	2.6893758E-8	54.426853	24
TTTGCCG	20	0.0069309454	52.51798	55
CTACGTG	20	0.006940078	52.500504	32
TTGGTAA	20	0.006940078	52.500504	33
GGCTCGT	20	0.006940078	52.500504	33
GACATAG	20	0.00694922	52.483036	1
TCGCCGT	55	2.2646418E-9	50.909573	52
TACGTGT	35	2.0786223E-5	50.000477	33
AGATCTC	75	1.4551915E-11	46.66711	40
TGGTCGC	60	4.8912625E-9	46.667107	49
TCTCGGT	70	3.8016879E-10	45.000427	43
TAGATCT	80	2.910383E-11	43.75042	39
CCGTATC	50	3.452511E-6	42.01438	55
ACGTGTA	50	3.460429E-6	42.0004	34
CGTGTAG	50	3.460429E-6	42.0004	35
GCCGTAT	60	2.5727968E-7	40.847313	54
>>END_MODULE