FastQCFastQC Report
Wed 25 May 2016
SRR1779801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150335
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1946212.945754481657632No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG28091.8684936974091195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC15101.0044234542854291No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4960.3299298233944191TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4130.27471979246349815No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT3080.20487577743040544No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT3080.20487577743040544No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1970.13104067582399306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC150.002224862869.970398
GGTCTAA150.002224862869.9703940
ACTCGTA700.059.97461738
AACTCGT750.055.97631537
GTAACTC750.055.97631535
GTTGCAC252.3893217E-455.97631538
CTCGTAT26950.054.00312439
CCGTCTT26850.053.99754747
CGTATGC27200.053.8926441
TCGTATG27350.053.85289440
CGTCTTC26950.053.79718448
GCCGTCT27050.053.72776846
TCTCGTA25350.053.68536838
TATGCCG27200.053.63539543
GTATGCC27200.053.63539542
ATCTCGT25400.053.5796937
TGCCGTC27200.053.5245845
CTGCTTG27150.053.4186854
ACGTATC25150.053.4167633
CGTATCT25300.053.3766234