FastQCFastQC Report
Wed 25 May 2016
SRR1779800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618512
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGC200.006949669752.50163749
ACCGACG350.001255126740.00124420
TCGCCGT601.214706E-535.00392552
GTAACCG501.7092585E-435.001095
TCCGGTG400.002418202335.001098
TGGTCGC853.9541465E-628.82442949
ACAACCG500.007206737528.0008728
TGTTCCG500.007206737528.0008725
TCGAGTA500.007206737528.00087224
CCGAACG500.007206737528.00087235
GTAGATC1653.6379788E-1227.57661838
GTCGCTT906.1680203E-627.2230748
TCGCTTA906.1680203E-627.2230749
GAACCGT657.9537043E-426.9239166
CGACGAT808.66248E-526.25081822
CGCTTAT959.380525E-625.79236450
GTTGTCG700.001226269224.99875617
ACATCGT851.3077876E-424.7066526
TCGGTGG1001.3979425E-524.49878345
GTCGCCG1202.1641372E-623.33594951