##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779800_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618512
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.46702893395763	34.0	31.0	34.0	21.0	34.0
2	31.59437650360866	34.0	33.0	34.0	23.0	34.0
3	31.167178648110305	34.0	33.0	34.0	23.0	34.0
4	31.19860730268774	34.0	33.0	34.0	23.0	34.0
5	31.833277284838452	34.0	34.0	34.0	23.0	34.0
6	34.63483651085185	38.0	36.0	38.0	26.0	38.0
7	34.8250252218227	38.0	36.0	38.0	26.0	38.0
8	34.24805824300903	38.0	35.0	38.0	23.0	38.0
9	35.152079506945704	38.0	36.0	38.0	27.0	38.0
10-11	33.66089340222987	37.5	33.5	38.0	19.0	38.0
12-13	34.66254090462271	38.0	36.0	38.0	25.5	38.0
14-15	33.415644320562905	37.5	33.0	38.0	17.0	38.0
16-17	34.11827579739762	37.5	35.0	38.0	24.0	38.0
18-19	35.145923603745764	38.0	36.5	38.0	27.0	38.0
20-21	33.64731807951988	37.5	33.0	38.0	18.5	38.0
22-23	34.65395497581292	38.0	35.5	38.0	25.0	38.0
24-25	34.989869234550014	38.0	36.5	38.0	26.5	38.0
26-27	33.842957776081946	38.0	34.0	38.0	22.0	38.0
28-29	35.233260631968335	38.0	36.5	38.0	29.0	38.0
30-31	35.65494444731873	38.0	37.0	38.0	31.0	38.0
32-33	35.75345829991981	38.0	37.0	38.0	31.5	38.0
34-35	35.650688264738605	38.0	37.0	38.0	31.0	38.0
36-37	35.120683996430145	38.0	36.5	38.0	28.5	38.0
38-39	35.090481672142175	38.0	36.5	38.0	28.5	38.0
40-41	35.10349273740849	38.0	36.5	38.0	27.0	38.0
42-43	35.49106484595286	38.0	36.5	38.0	29.5	38.0
44-45	35.52718459787361	38.0	37.0	38.0	31.0	38.0
46-47	35.43957109967147	38.0	37.0	38.0	29.5	38.0
48-49	35.18471104845177	38.0	36.5	38.0	29.0	38.0
50-51	34.510056393408696	38.0	35.0	38.0	24.5	38.0
52-53	35.07653933957627	38.0	36.0	38.0	27.0	38.0
54-55	35.25019239723724	38.0	36.5	38.0	29.0	38.0
56-57	35.316934028765814	38.0	37.0	38.0	29.0	38.0
58-59	35.60037396202499	38.0	37.0	38.0	31.0	38.0
60-61	35.4315486199136	38.0	37.0	38.0	30.5	38.0
62-63	35.4854748169801	38.0	37.0	38.0	31.0	38.0
64-65	35.494076105233205	38.0	37.0	38.0	29.5	38.0
66-67	35.26578142380423	38.0	37.0	38.0	27.0	38.0
68-69	34.62276075484388	38.0	36.0	38.0	24.5	38.0
70-71	34.68286629847117	38.0	35.5	38.0	25.5	38.0
72-73	34.98749417957937	38.0	36.0	38.0	27.0	38.0
74-75	35.11729279302584	38.0	36.5	38.0	28.5	38.0
76	34.141454005742816	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	17.0
13	221.0
14	998.0
15	2002.0
16	2811.0
17	3399.0
18	3614.0
19	3391.0
20	3163.0
21	3029.0
22	3004.0
23	3485.0
24	3984.0
25	4661.0
26	5886.0
27	7333.0
28	8863.0
29	10734.0
30	13730.0
31	17255.0
32	22263.0
33	29631.0
34	41587.0
35	62996.0
36	114559.0
37	245895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.19063555965142	21.16069263229374	11.454948181921068	28.19372362613377
2	18.68047830923248	22.15478438575161	35.98475049796932	23.17998680704659
3	18.76341930310164	24.121924877771168	31.542961171327317	25.57169464779988
4	13.423021703701787	18.816773158806942	35.31378534288744	32.44641979460383
5	13.973536487570168	34.64977235688232	34.863187779703544	16.51350337584396
6	29.172244750134197	38.601792747521394	17.487049145340603	14.73891335700381
7	25.55038514024797	30.40247317600036	23.315860275903997	20.731281407847675
8	22.237995149555374	36.99288601455134	21.734680679062247	19.034438156831044
9	23.094025026557773	17.00910159540668	23.059746500599065	36.83712687743648
10-11	24.022535147505945	25.790520783734213	26.64201564913852	23.544928419621325
12-13	23.343562200187222	24.149853438338205	27.851404421263325	24.65517994021125
14-15	22.712199822476308	25.94626755633305	25.049675914174248	26.291856707016386
16-17	22.85007518067614	28.326138623466075	25.69319816979515	23.130588026062636
18-19	21.265936780768264	27.831191183163746	27.891256001429266	23.011616034638728
20-21	22.875708151175726	27.054042605478955	27.422103370670253	22.648145872675066
22-23	22.970387155344756	27.56878149259861	26.634179387662215	22.82665196439442
24-25	21.94991224659233	28.248755647786684	26.609290785932615	23.19204131968837
26-27	22.06875254645049	28.19526990758406	27.207004598162026	22.528972947803425
28-29	21.521647037518512	27.97986314570843	27.977437797913556	22.521052018859507
30-31	22.28284286660108	28.147748741126854	27.15689896436514	22.41250942790693
32-33	21.45924412137517	28.534854618827122	27.44441498305611	22.5614862767416
34-35	21.575524359632496	28.091043949603268	27.72612440732911	22.607307283435127
36-37	22.564476980072737	27.920080724580732	27.19870180902621	22.31674048632032
38-39	22.041029165066597	27.53820967318148	28.087313297087533	22.33344786466439
40-41	21.78547263295492	27.629773883463205	27.732844415234393	22.851909068347485
42-43	21.01447107926394	27.409522408305776	28.285014215626436	23.290992296803847
44-45	21.07331451318219	26.236810534110717	29.014965577232143	23.67490937547495
46-47	21.005305590878557	26.880016685569974	28.99376473831384	23.120912985237634
48-49	20.503984161975968	26.748734663785555	29.365177527014453	23.38210364722402
50-51	21.199272433306387	26.662004850444625	28.81794664510913	23.320776071139854
52-53	19.466282051696652	27.131605584247172	29.614571172633696	23.78754119142248
54-55	19.868644012986845	27.085462235962176	28.937526678653196	24.10836707239778
56-57	19.826967295023916	27.344293203199026	28.69333708471989	24.13540241705717
58-59	19.3419004846814	27.064221296067974	29.13303069379373	24.46084752545689
60-61	19.56089325305764	26.879500751169612	28.729609666720034	24.82999632905272
62-63	19.88628141186971	26.89906242395383	28.736055001443113	24.478601162733355
64-65	20.341226448809564	26.33391387593853	28.14491572429563	25.17994395095628
66-67	20.167251905496126	27.155593753966834	27.244370024021336	25.4327843165157
68-69	20.571178200549383	27.240097589210215	27.07356861882812	25.11515559141228
70-71	20.74097427689124	27.368757174500008	26.904253771159723	24.986014777449032
72-73	20.70937798865131	27.7059850230927	26.789876238003963	24.794760750252028
74-75	21.414365659273585	28.0723440928369	25.96970544626096	24.54358480162856
76	21.668517916705067	28.593648191352965	25.370694631051528	24.367139260890443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	3.5
7	4.0
8	3.0
9	4.5
10	7.0
11	15.5
12	23.0
13	35.5
14	71.0
15	142.0
16	276.5
17	363.0
18	473.0
19	795.5
20	1252.0
21	1496.0
22	1902.5
23	2775.5
24	3832.0
25	4422.0
26	5093.5
27	6670.0
28	8540.0
29	9505.0
30	10413.0
31	12416.5
32	14734.5
33	15957.0
34	17366.5
35	20076.5
36	22730.0
37	24083.0
38	25447.0
39	28020.0
40	29840.0
41	31004.5
42	31558.0
43	31672.0
44	32216.0
45	32047.0
46	31448.0
47	30896.0
48	29443.0
49	27645.5
50	26749.0
51	25723.0
52	23611.0
53	21766.0
54	21007.0
55	19724.5
56	17332.0
57	14993.0
58	13764.0
59	12636.0
60	10440.0
61	8431.5
62	7491.0
63	6770.0
64	5374.0
65	4296.0
66	3516.0
67	3139.0
68	2835.5
69	2284.0
70	1903.0
71	1770.0
72	1585.5
73	1285.5
74	1039.0
75	908.0
76	861.5
77	775.5
78	668.5
79	601.0
80	585.5
81	511.0
82	432.0
83	412.0
84	341.0
85	242.0
86	189.0
87	164.0
88	145.0
89	107.0
90	76.5
91	53.5
92	42.0
93	34.0
94	23.0
95	16.0
96	12.0
97	10.5
98	7.5
99	14.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.233567012442766E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0032335670124427657
7	0.005820420622396978
8	0.0019401402074656596
9	0.007922239180484777
10-11	0.0018593010321545905
12-13	1.616783506221383E-4
14-15	4.850350518664149E-4
16-17	3.233567012442766E-4
18-19	0.002182657733398867
20-21	0.0
22-23	0.002182657733398867
24-25	0.00282937113588742
26-27	6.467134024885532E-4
28-29	0.007113847427374085
30-31	4.041958765553457E-4
32-33	0.0
34-35	0.003152727837131697
36-37	0.018269653620301626
38-39	0.008003078355795846
40-41	5.65874227177484E-4
42-43	4.041958765553457E-4
44-45	0.004203637116175596
46-47	0.002182657733398867
48-49	8.083917531106915E-5
50-51	0.0019401402074656596
52-53	0.009377344336084021
54-55	0.006467134024885531
56-57	0.011964197946038233
58-59	0.010428253615127921
60-61	0.023120004138965776
62-63	0.008973148459528674
64-65	0.020209793827767285
66-67	0.016733709289391316
68-69	1.616783506221383E-4
70-71	3.233567012442766E-4
72-73	0.004284476291486665
74-75	0.008245595881729054
76	0.0017784618568435213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	618512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.26829946695067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79896524971947	44.79330135369485
2	14.458054583353347	18.294730541782364
3	7.730364737275123	14.672610935600341
4	3.8106136838500624	9.643641908107433
5	1.7461606697017011	5.523830808404919
6	0.7193862075921522	2.730860520860054
7	0.29584589091749064	1.3102366495844235
8	0.15537047175425367	0.7864020428215617
9	0.08234624187517095	0.4688916021842643
>10	0.20289226396125745	1.7754936369597918
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.083917531106914E-4	0.0
2	0.0	0.0	0.0	0.001131748454354968	0.0
3	0.0	0.0	0.0	0.0012934268049771063	0.0
4	0.0	0.0	0.0	0.0012934268049771063	0.0
5	0.0	0.0	0.0	0.0012934268049771063	0.0
6	0.0	0.0	0.0	0.0012934268049771063	0.0
7	0.0	0.0	0.0	0.0016167835062213828	0.0
8	0.0	0.0	0.0	0.0017784618568435213	0.0
9	0.0	0.0	0.0	0.003395245363064904	0.0
10	0.0	0.0	0.0	0.0035569237136870427	0.0
11	0.0	0.0	0.0	0.003880280414931319	0.0
12	0.0	0.0	0.0	0.0040419587655534574	0.0
13	0.0	0.0	0.0	0.004203637116175596	0.0
14	0.0	0.0	0.0	0.005012028869286287	0.0
15	0.0	0.0	0.0	0.005173707219908425	0.0
16	0.0	0.0	0.0	0.0053353855705305635	0.0
17	0.0	0.0	0.0	0.006305455674263393	0.0
18	0.0	0.0	0.0	0.006467134024885531	0.0
19	0.0	0.0	0.0	0.0075988824792405	0.0
20	0.0	0.0	0.0	0.008892309284217605	0.0
21	0.0	0.0	0.0	0.01050909279043899	0.0
22	0.0	0.0	0.0	0.014066016504126031	0.0
23	0.0	0.0	0.0	0.015359443309103138	0.0
24	0.0	0.0	0.0	0.01891636702279018	0.0
25	0.0	0.0	0.0	0.021664898983366532	0.0
26	0.0	0.0	0.0	0.024090074242698607	0.0
27	0.0	0.0	0.0	0.033629096929404766	0.0
28	0.0	0.0	0.0	0.04526993817419872	0.0
29	0.0	0.0	0.0	0.06709651550818739	0.0
30	0.0	0.0	0.0	0.09458183511395091	0.0
31	0.0	0.0	0.0	0.12174379801847013	0.0
32	0.0	0.0	0.0	0.15375611144165352	0.0
33	0.0	0.0	0.0	0.21179863931500117	0.0
34	0.0	0.0	0.0	0.29247613627544816	0.0
35	0.0	0.0	0.0	0.4093695837752542	0.0
36	0.0	0.0	0.0	0.591581084926404	0.0
37	0.0	0.0	0.0	0.8984465944072225	0.0
38	0.0	0.0	0.0	1.2877680627053314	0.0
39	0.0	0.0	0.0	1.7065149908166697	0.0
40	0.0	0.0	0.0	2.2251791396124894	0.0
41	0.0	0.0	0.0	2.7819993791551334	0.0
42	0.0	0.0	0.0	3.451347750730786	0.0
43	0.0	0.0	0.0	4.179223685231653	0.0
44	0.0	0.0	0.0	5.0217295703236156	0.0
45	0.0	0.0	0.0	5.930846935871899	0.0
46	0.0	0.0	0.0	6.814095765320641	0.0
47	0.0	0.0	0.0	7.644475774115943	0.0
48	0.0	0.0	0.0	8.372190030266188	0.0
49	0.0	0.0	0.0	9.070155469901959	0.0
50	0.0	0.0	0.0	9.788977416767985	0.0
51	0.0	0.0	0.0	10.494218382181753	0.0
52	0.0	0.0	0.0	11.28126859301032	0.0
53	0.0	0.0	0.0	12.074947616214398	0.0
54	0.0	0.0	0.0	12.877842305403938	0.0
55	0.0	0.0	0.0	13.717923015236568	0.0
56	0.0	0.0	0.0	14.470212380681378	0.0
57	0.0	0.0	0.0	15.155890265669866	0.0
58	0.0	0.0	0.0	15.791771218666735	0.0
59	0.0	0.0	0.0	16.377531882970743	0.0
60	0.0	0.0	0.0	17.015191297824455	0.0
61	0.0	0.0	0.0	17.630862456993558	0.0
62	0.0	0.0	0.0	18.25106060998008	0.0
63	0.0	0.0	0.0	18.872875546472827	0.0
64	0.0	0.0	0.0	19.511666709780894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGC	20	0.0069496697	52.501637	49
ACCGACG	35	0.0012551267	40.001244	20
TCGCCGT	60	1.214706E-5	35.003925	52
GTAACCG	50	1.7092585E-4	35.00109	5
TCCGGTG	40	0.0024182023	35.00109	8
TGGTCGC	85	3.9541465E-6	28.824429	49
ACAACCG	50	0.0072067375	28.000872	8
TGTTCCG	50	0.0072067375	28.000872	5
TCGAGTA	50	0.0072067375	28.000872	24
CCGAACG	50	0.0072067375	28.000872	35
GTAGATC	165	3.6379788E-12	27.576618	38
GTCGCTT	90	6.1680203E-6	27.22307	48
TCGCTTA	90	6.1680203E-6	27.22307	49
GAACCGT	65	7.9537043E-4	26.923916	6
CGACGAT	80	8.66248E-5	26.250818	22
CGCTTAT	95	9.380525E-6	25.792364	50
GTTGTCG	70	0.0012262692	24.998756	17
ACATCGT	85	1.3077876E-4	24.706652	6
TCGGTGG	100	1.3979425E-5	24.498783	45
GTCGCCG	120	2.1641372E-6	23.335949	51
>>END_MODULE