FastQCFastQC Report
Wed 25 May 2016
SRR1779800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618512
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT140772.275946141707841No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19870.3212548826861888No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC10960.17719947228186358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA200.006955161752.4911543
CTCGTAT20350.050.38462439
CCGGTCA352.0884305E-549.99156618
TATGCCG20700.049.8708243
CGTATGC20850.049.8477141
TCGTATG20850.049.8477140
TCTCGTA20000.049.5166538
CGTCTTC20750.049.43733648
ATCTCGT20000.049.34168237
ATGCCGT20950.049.275744
TGCCGTC20950.049.12057545
CCGTCTT21100.048.77926647
GCCGTCT21250.048.4349446
GTATGCC21700.047.73388742
CCGCGTG305.882172E-446.658840
GATCTCG21150.046.49334336
GTAGATC20700.046.32069433
GTCTTCT23100.044.71473749
CTGCTTG23200.044.52200354
TAGATCT21650.044.28814734
Produced by FastQC (version 0.11.5)