##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779799_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70210
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.690457199829083	32.0	21.0	34.0	12.0	34.0
2	26.954678820680815	33.0	21.0	34.0	12.0	34.0
3	26.47617148554337	33.0	21.0	34.0	11.0	34.0
4	26.88346389403219	33.0	21.0	34.0	12.0	34.0
5	27.02684802734653	34.0	21.0	34.0	11.0	34.0
6	29.456430707876372	36.0	21.0	38.0	11.0	38.0
7	29.595114656031903	36.0	21.0	38.0	12.0	38.0
8	29.207577268195415	34.0	21.0	38.0	11.0	38.0
9	29.736319612590798	36.0	21.0	38.0	11.0	38.0
10-11	28.469911693490957	34.0	21.0	38.0	11.0	38.0
12-13	28.977367896311065	34.5	21.0	38.0	11.0	38.0
14-15	28.03886910696482	34.0	21.0	38.0	11.0	38.0
16-17	28.378343540806153	34.5	21.0	38.0	11.0	38.0
18-19	28.814577695484974	35.5	21.0	38.0	11.0	38.0
20-21	28.17021079618288	33.0	16.0	38.0	11.0	38.0
22-23	28.72536675687224	35.0	21.0	38.0	11.0	38.0
24-25	28.98498789346247	35.0	21.0	38.0	11.0	38.0
26-27	28.039716564591938	34.0	21.0	38.0	11.0	38.0
28-29	28.76691354507905	35.0	21.0	38.0	11.0	38.0
30-31	29.009692351516875	36.0	21.0	38.0	11.0	38.0
32-33	29.182794473721692	36.5	21.0	38.0	11.0	38.0
34-35	29.205398091439967	36.0	21.0	38.0	11.0	38.0
36-37	28.829661016949153	35.5	21.0	38.0	11.0	38.0
38-39	28.68118501637943	35.0	21.0	38.0	11.0	38.0
40-41	28.686191425722832	35.0	21.0	38.0	11.0	38.0
42-43	28.992422731804588	35.5	21.0	38.0	11.0	38.0
44-45	29.08497365047714	36.0	21.0	38.0	11.0	38.0
46-47	29.03595641646489	36.0	21.0	38.0	11.0	38.0
48-49	28.736910696481985	35.5	21.0	38.0	11.0	38.0
50-51	28.259044295684376	34.5	16.0	38.0	11.0	38.0
52-53	28.709143996581684	34.5	21.0	38.0	11.0	38.0
54-55	28.797799458766555	35.5	21.0	38.0	11.0	38.0
56-57	28.80399515738499	36.0	21.0	38.0	11.0	38.0
58-59	28.85995584674548	36.0	21.0	38.0	11.0	38.0
60-61	28.88352086597351	36.0	21.0	38.0	11.0	38.0
62-63	28.792629255091867	36.0	21.0	38.0	11.0	38.0
64-65	28.850740635237145	36.0	21.0	38.0	11.0	38.0
66-67	28.874540663723117	36.0	21.0	38.0	11.0	38.0
68-69	28.446966244124766	34.0	21.0	38.0	11.0	38.0
70-71	28.594117647058823	34.0	21.0	38.0	11.0	38.0
72-73	28.595157384987893	34.5	20.5	38.0	11.0	38.0
74-75	28.562427004700186	35.0	20.0	38.0	11.0	38.0
76	27.81506907847885	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	43.0
13	423.0
14	1514.0
15	2617.0
16	3512.0
17	4124.0
18	4087.0
19	3618.0
20	2810.0
21	2020.0
22	1349.0
23	1014.0
24	859.0
25	717.0
26	682.0
27	683.0
28	820.0
29	877.0
30	1060.0
31	1285.0
32	1601.0
33	2224.0
34	2928.0
35	4416.0
36	7983.0
37	16944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.31049708018801	19.54137587238285	9.933057968950292	40.21506907847885
2	15.912263210368893	21.34453781512605	35.96638655462185	26.776812419883207
3	21.273322888477423	20.43725964962256	32.03674690215069	26.252670559749326
4	12.726107392109387	26.232730380287713	30.65090442956844	30.39025779803447
5	13.602050989887479	30.22931206380857	37.15710012818687	19.01153681811708
6	21.88243337558933	44.3502784622616	15.140940362072158	18.626347800076914
7	20.1475741431868	35.93060026779864	17.3271416768753	26.594683912139256
8	17.711909068896265	38.85082684062843	15.670802056774965	27.766462033700346
9	22.07834757834758	19.17948717948718	17.03988603988604	41.7022792022792
10-11	20.02578090503796	29.559018331505406	22.264161693278446	28.151039070178186
12-13	19.217347956131604	23.583535108958838	25.518444666001994	31.68067226890756
14-15	25.567234969875656	25.775185517526246	20.827814097907677	27.82976541469042
16-17	27.38486080745209	24.861662049466947	20.370040664591894	27.38343647848907
18-19	21.40201692163063	24.946585761900693	23.313534455744524	30.337862860724158
20-21	24.78849166785358	23.994445235721408	22.167070217917676	29.049992878507336
22-23	26.965587967183225	24.07275524156791	20.82668641750228	28.134970373746583
24-25	21.352267547857796	29.325005697356428	20.234161349134002	29.088565405651778
26-27	23.577293671084398	29.97600039880643	21.668720045008154	24.77798588510102
28-29	22.254271449835837	27.904906380645116	24.15657115996838	25.684251009550668
30-31	23.371481067376923	26.36110497867098	24.006722736951552	26.26069121700055
32-33	19.95655889474434	29.270759151118074	24.004415325452214	26.76826662868537
34-35	19.835487661574618	31.68607342520386	22.4035893601111	26.074849553110425
36-37	24.130979855828134	25.599054050203723	23.202125537795254	27.06784055617289
38-39	24.58155866725545	23.670602982863493	25.409894445948062	26.33794390393299
40-41	20.987337805694427	27.321283596120153	24.57234827443775	27.119030323747666
42-43	17.872352547394208	26.67677933028529	25.782307111623865	29.668561010696635
44-45	17.475126592645875	24.29225630470547	28.94573786954013	29.286879233108515
46-47	19.091401142336235	26.717421339752445	26.2609141538593	27.930263364052017
48-49	20.06053268765133	27.496083179034326	24.71727674120496	27.726107392109384
50-51	24.038571103008206	27.106597538742022	21.79737351868733	27.057457839562442
52-53	16.364206147972993	29.773368280105977	24.938748183812425	28.923677388108597
54-55	18.188228255605253	26.197259337340817	24.57123728668699	31.043275120366943
56-57	20.19629350009259	27.896326263158645	22.590134043674592	29.31724619307417
58-59	19.20285468052222	27.770457475374105	22.870919722793996	30.155768121309677
60-61	17.62204286904923	25.545479801397626	24.338051445708466	32.49442588384468
62-63	19.61227004351635	26.114082631207847	23.260236313004338	31.01341101227147
64-65	20.145171170914477	25.314490048865274	22.448819682874362	32.09151909734589
66-67	17.86090771816861	30.57636969714237	20.22992108037266	31.33280150431636
68-69	18.73606848076115	33.13440488822737	19.480981918401355	28.648544712610118
70-71	17.649320960838633	33.575940577842026	20.98149110875309	27.793247352566247
72-73	19.046262534184137	33.8836030082042	20.341698951686418	26.72843550592525
74-75	20.51933992835217	33.570497617674086	19.287225177873214	26.622937276100533
76	18.990983805033686	36.25279530531144	18.426937484866183	26.329283404788693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	2.0
15	8.0
16	18.0
17	23.0
18	22.0
19	22.0
20	46.5
21	70.0
22	80.5
23	85.0
24	106.5
25	134.0
26	174.5
27	233.0
28	308.5
29	366.0
30	416.5
31	554.5
32	775.5
33	909.0
34	1018.0
35	1243.0
36	1550.5
37	1742.0
38	1892.0
39	2307.5
40	2735.5
41	3061.5
42	3225.0
43	3345.0
44	3612.5
45	3903.5
46	4047.0
47	3998.0
48	4030.0
49	4148.5
50	4186.0
51	3984.5
52	3576.5
53	3242.0
54	3114.0
55	2905.0
56	2567.0
57	2200.0
58	1962.0
59	1847.0
60	1629.0
61	1369.0
62	1212.0
63	1149.5
64	981.5
65	807.0
66	691.0
67	644.0
68	571.0
69	462.5
70	421.5
71	416.0
72	383.5
73	313.0
74	255.0
75	235.0
76	238.0
77	211.0
78	159.5
79	138.0
80	127.0
81	103.0
82	78.0
83	66.0
84	62.0
85	46.5
86	32.0
87	29.0
88	26.0
89	18.5
90	12.5
91	10.0
92	9.0
93	8.0
94	5.5
95	3.5
96	3.0
97	3.0
98	3.5
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.004272895598917533
7	0.011394388263780088
8	0.004272895598917533
9	0.01424298532972511
10-11	0.004272895598917533
12-13	0.0
14-15	0.001424298532972511
16-17	0.0021364477994587666
18-19	0.005697194131890044
20-21	0.0
22-23	0.002848597065945022
24-25	0.002848597065945022
26-27	7.121492664862555E-4
28-29	0.007833641931348811
30-31	7.121492664862555E-4
32-33	0.0
34-35	0.0035607463324312774
36-37	0.022788776527560176
38-39	0.0128186867967526
40-41	0.001424298532972511
42-43	0.001424298532972511
44-45	0.0064093433983763
46-47	0.004272895598917533
48-49	0.0
50-51	0.002848597065945022
52-53	0.011394388263780088
54-55	0.011394388263780088
56-57	0.0128186867967526
58-59	0.013530836063238856
60-61	0.027773821392963964
62-63	0.009257940464321322
64-65	0.024213075060532687
66-67	0.017091582395670133
68-69	7.121492664862555E-4
70-71	7.121492664862555E-4
72-73	0.002848597065945022
74-75	0.007833641931348811
76	0.004272895598917533
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	70210.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.87337986041875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.322841612944	68.08574277168495
2	6.878306878306878	10.850306224184589
3	3.470755006591184	8.212505341119499
4	1.794969030463911	5.663010967098704
5	0.8505336150387346	3.3542230451502633
6	0.3593549668634993	1.7006124483691782
7	0.14085270057966304	0.7776669990029911
8	0.08667858497210033	0.5469306366614443
9	0.04875670404680643	0.3461045435123202
>10	0.04695090019322101	0.4628970232160661
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.005697194131890044	0.0
5	0.0	0.0	0.0	0.009970089730807577	0.0
6	0.0	0.0	0.0	0.009970089730807577	0.0
7	0.0	0.0	0.0	0.009970089730807577	0.0
8	0.0	0.0	0.0	0.009970089730807577	0.0
9	0.0	0.0	0.0	0.009970089730807577	0.0
10	0.0	0.0	0.0	0.01424298532972511	0.0
11	0.0	0.0	0.0	0.017091582395670133	0.0
12	0.0	0.0	0.0	0.017091582395670133	0.0
13	0.0	0.0	0.0	0.017091582395670133	0.0
14	0.0	0.0	0.0	0.017091582395670133	0.0
15	0.0	0.0	0.0	0.018515880928642644	0.0
16	0.0	0.0	0.0	0.018515880928642644	0.0
17	0.0	0.0	0.0	0.018515880928642644	0.0
18	0.0	0.0	0.0	0.018515880928642644	0.0
19	0.0	0.0	0.0	0.025637373593505198	0.0
20	0.0	0.0	0.0	0.02991026919242273	0.0
21	0.0	0.0	0.0	0.02991026919242273	0.0
22	0.0	0.0	0.0	0.05269904571998291	0.0
23	0.0	0.0	0.0	0.05269904571998291	0.0
24	0.0	0.0	0.0	0.07548782224754308	0.0
25	0.0	0.0	0.0	0.0783364193134881	0.0
26	0.0	0.0	0.0	0.08260931491240564	0.0
27	0.0	0.0	0.0	0.0911551061102407	0.0
28	0.0	0.0	0.0	0.1410055547642786	0.0
29	0.0	0.0	0.0	0.18658310781939894	0.0
30	0.0	0.0	0.0	0.22076627261073922	0.0
31	0.0	0.0	0.0	0.25637373593505197	0.0
32	0.0	0.0	0.0	0.27916251246261214	0.0
33	0.0	0.0	0.0	0.3190428713858425	0.0
34	0.0	0.0	0.0	0.4116222760290557	0.0
35	0.0	0.0	0.0	0.5227175616009115	0.0
36	0.0	0.0	0.0	0.7121492664862555	0.0
37	0.0	0.0	0.0	1.1551061102407065	0.0
38	0.0	0.0	0.0	1.5197265346816693	0.0
39	0.0	0.0	0.0	1.9171058253809998	0.0
40	0.0	0.0	0.0	2.3728813559322033	0.0
41	0.0	0.0	0.0	2.8030195128899016	0.0
42	0.001424298532972511	0.0	0.0	3.2901296111665004	0.0
43	0.001424298532972511	0.0	0.0	3.8812135023500924	0.0
44	0.001424298532972511	0.0	0.0	4.53211793191853	0.0
45	0.001424298532972511	0.0	0.0	5.151687793761573	0.0
46	0.001424298532972511	0.0	0.0	5.761287565873807	0.0
47	0.001424298532972511	0.0	0.0	6.316763993733087	0.0
48	0.001424298532972511	0.0	0.0	6.838057256801026	0.0
49	0.001424298532972511	0.0	0.0	7.228315054835494	0.0
50	0.001424298532972511	0.0	0.0	7.6456345249964395	0.0
51	0.001424298532972511	0.0	0.0	8.057256801025495	0.0
52	0.001424298532972511	0.0	0.0	8.567155675829653	0.0
53	0.001424298532972511	0.0	0.0	9.057114371172197	0.0
54	0.001424298532972511	0.0	0.0	9.594074918102834	0.0
55	0.001424298532972511	0.0	0.0	10.03418316479134	0.0
56	0.001424298532972511	0.0	0.0	10.383136305369606	0.0
57	0.001424298532972511	0.0	0.0	10.833214641788919	0.0
58	0.001424298532972511	0.0	0.0	11.20780515596069	0.0
59	0.001424298532972511	0.0	0.0	11.632246118786497	0.0
60	0.001424298532972511	0.0	0.0	12.036746902150691	0.0
61	0.001424298532972511	0.0	0.0	12.397094430992736	0.0
62	0.001424298532972511	0.0	0.0	12.76741204956559	0.0
63	0.001424298532972511	0.0	0.0	13.086454920951432	0.0
64	0.001424298532972511	0.0	0.0	13.488107107249679	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTCT	15	0.0022134099	70.022095	34
TCGTGTA	15	0.0022134099	70.022095	34
TCAGCTC	15	0.0022134099	70.022095	38
ACGTGTC	15	0.0022134099	70.022095	34
ACGTGTA	30	1.0081203E-7	70.022095	34
CGTGTCG	15	0.0022134099	70.022095	10
GACGCGA	15	0.0022134099	70.022095	23
CACGTGT	25	2.8079958E-6	70.022095	33
TACGTGT	15	0.0022134099	70.022095	33
CGCGAGG	15	0.0022196798	69.97223	11
GGTGGAC	20	7.892455E-5	69.97222	47
GTGTACA	35	2.9291732E-7	60.01894	36
GACGACT	35	2.9291732E-7	60.01894	23
CGACGCG	30	8.283187E-6	58.35175	22
TCTCGGT	60	1.8189894E-12	58.31019	43
CTCTCGG	30	8.318302E-6	58.31019	42
ACGACTC	30	8.318302E-6	58.31019	24
CTCGGTG	55	3.45608E-11	57.25	44
CTTCCGA	50	9.567884E-10	56.017677	18
AGTGTAC	25	2.3718386E-4	56.017677	35
>>END_MODULE