FastQCFastQC Report
Wed 25 May 2016
SRR1779799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70210
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1897427.024640364620424No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG24573.4995014955134596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14942.1279020082609312No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4300.6124483691781798Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC3750.5341119498646917No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT3160.4500783364193135No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT3130.44580544082039597No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1640.23358495940749183No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1530.2179176755447942No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1330.18943170488534397No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1270.18088591368750892No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1220.17376442102264633No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG920.13103546503347102No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG810.1153681811707734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA150.002206262770.0791857
TCGAAAA150.002206262770.0791858
TAAGAGA308.313287E-658.3161244
GAGCTCG252.379018E-455.9834836
GCTCGTA252.379018E-455.9834838
CAGAGCT252.379018E-455.9834834
AGAGCTC252.379018E-455.9834835
CGTCTTC27500.052.67535848
GTCTTCT27500.052.5855849
TGCTCGA200.006894085652.5593855
AGCAATT200.00691357252.52191567
TGGAGAT200.00691357252.52191567
CAGATAA200.00691357252.52191559
AAGCAAT200.00691357252.52191566
AAACACG200.00691357252.52191569
CCGTCTT27650.052.51614447
CTTAATA200.006933099652.484511
TTTAGTG200.006933099652.4845123
CCTTATA407.3982847E-752.484511
TGAAGGC200.006933099652.4845121
Produced by FastQC (version 0.11.5)