##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779799_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70210
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.6056544651759	34.0	34.0	34.0	34.0	34.0
2	33.67147130038456	34.0	34.0	34.0	34.0	34.0
3	33.77068793619143	34.0	34.0	34.0	34.0	34.0
4	33.59275032046717	34.0	34.0	34.0	34.0	34.0
5	33.63975217205526	34.0	34.0	34.0	34.0	34.0
6	37.35704315624555	38.0	38.0	38.0	37.0	38.0
7	37.41541091012676	38.0	38.0	38.0	37.0	38.0
8	37.32351516877937	38.0	38.0	38.0	37.0	38.0
9	37.24009400370318	38.0	38.0	38.0	36.0	38.0
10-11	37.18112804443811	38.0	38.0	38.0	36.5	38.0
12-13	37.32770260646632	38.0	38.0	38.0	36.5	38.0
14-15	37.36588805013531	38.0	38.0	38.0	37.0	38.0
16-17	37.21412191995442	38.0	38.0	38.0	36.0	38.0
18-19	37.05185158809286	38.0	38.0	38.0	35.5	38.0
20-21	37.115688648340694	38.0	38.0	38.0	35.5	38.0
22-23	37.172824383990886	38.0	38.0	38.0	36.0	38.0
24-25	37.25381712006836	38.0	38.0	38.0	36.0	38.0
26-27	37.11999002991027	38.0	38.0	38.0	36.0	38.0
28-29	36.666763993733085	38.0	37.0	38.0	34.0	38.0
30-31	37.03534396809572	38.0	37.5	38.0	36.0	38.0
32-33	36.96524711579547	38.0	38.0	38.0	35.5	38.0
34-35	37.00600341831648	38.0	38.0	38.0	36.0	38.0
36-37	36.94547785215781	38.0	38.0	38.0	35.5	38.0
38-39	35.126385130323314	38.0	35.5	38.0	28.5	38.0
40-41	36.37386412191995	38.0	37.0	38.0	34.0	38.0
42-43	37.125302663438255	38.0	37.5	38.0	36.0	38.0
44-45	36.935543369890325	38.0	37.5	38.0	35.0	38.0
46-47	37.092600769121205	38.0	38.0	38.0	36.0	38.0
48-49	36.71543227460476	38.0	37.0	38.0	34.5	38.0
50-51	36.78720267768124	38.0	37.0	38.0	34.5	38.0
52-53	36.70278450363196	38.0	37.0	38.0	34.5	38.0
54-55	36.72046004842615	38.0	37.0	38.0	35.0	38.0
56-57	36.59099131177895	38.0	37.0	38.0	34.0	38.0
58-59	36.61425010682239	38.0	37.0	38.0	34.0	38.0
60-61	36.23725252812989	38.0	37.0	38.0	34.0	38.0
62-63	36.15707164221621	38.0	37.0	38.0	34.0	38.0
64-65	36.589510041304656	38.0	37.0	38.0	34.0	38.0
66-67	36.26188577125765	38.0	37.0	38.0	33.0	38.0
68-69	36.19607605754166	38.0	37.0	38.0	34.0	38.0
70-71	33.98765133171913	38.0	33.0	38.0	21.0	38.0
72-73	30.07372881355932	37.0	22.5	38.0	11.0	38.0
74-75	29.49074918102834	37.0	22.0	38.0	11.0	38.0
76	29.461259079903147	37.0	22.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	11.0
24	19.0
25	38.0
26	106.0
27	160.0
28	314.0
29	504.0
30	834.0
31	1256.0
32	1952.0
33	3107.0
34	5623.0
35	12955.0
36	13917.0
37	29412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.69151634322191	13.06032885925166	12.192584993303127	50.05556980422331
2	12.157812277453354	12.773109243697478	54.55205811138014	20.517020367469023
3	12.21193562170631	14.809856145848169	53.305796895029204	19.672411337416325
4	9.282153539381854	40.7619997151403	29.82481128044438	20.13103546503347
5	9.034325594644638	26.87366472012534	54.30992736077482	9.782082324455207
6	18.713858424725824	55.449366187152826	15.455063381284717	10.381712006836633
7	17.309500071214927	23.5336846603048	16.755447941888622	42.401367326591654
8	14.834069220908702	55.14883919669563	12.965389545648767	17.051702036746903
9	15.395242842899872	14.93519441674975	13.062241845890899	56.60732089445948
10-11	16.50619569861843	34.18031619427432	31.290414470873095	18.023073636234155
12-13	16.197122916963398	15.586810995584674	34.367611451360204	33.848454636091724
14-15	15.098276598775104	14.665289844751461	17.88918957413474	52.347243982338696
16-17	32.14641788918958	31.536818117077342	15.098276598775104	21.218487394957982
18-19	33.78080045577553	19.490813274462326	15.514171770403076	31.214214499359066
20-21	16.6165788349238	15.812562313060818	15.569719413189004	52.001139438826385
22-23	15.450078336419311	33.72952570858852	15.141005554764279	35.67939040022789
24-25	32.464979810424516	33.121586110141784	15.20591942685819	19.207514652575504
26-27	33.960974220196555	35.03560746332431	16.018373451075345	14.985044865403788
28-29	31.303233157669847	20.03774391112377	16.90215069078479	31.75687224042159
30-31	18.722404215923657	16.399373308645494	48.1348810710725	16.743341404358354
32-33	14.98290841760433	48.8135593220339	20.99273607748184	15.210796182879932
34-35	14.897450505625978	37.19769263637659	16.061814556331008	31.84304230166643
36-37	31.83307221193562	34.77638513032332	16.19783506622988	17.19270759151118
38-39	16.378008830650902	19.772824383990883	32.95826805298391	30.890898732374307
40-41	14.534966528984475	15.51345962113659	35.647343683236	34.30423016664293
42-43	32.01204965140045	15.069185793945264	33.76537363177348	19.153390922880806
44-45	15.72425580401652	32.224042159236575	36.34809856145848	15.703603475288421
46-47	31.42216208517305	16.30465745620282	21.4869676684233	30.78621279020083
48-49	30.739210938612732	15.074775672981055	17.313772966813843	36.872240421592366
50-51	16.980997692373435	15.200421640409106	35.07521153243498	32.74336913478248
52-53	13.053234458145448	15.214526389274749	52.160935752498595	19.57130340008121
54-55	12.79175810082932	15.393713129470774	38.59614124084471	33.218387528855196
56-57	30.262332924848245	15.587506056028955	21.31935934338397	32.83080167573883
58-59	14.552759809648647	16.0024791268914	49.54193143931838	19.902829624141567
60-61	30.22904570227621	31.462971538488937	21.918569443949703	16.38941331528515
62-63	13.732921112994914	52.73119719614184	16.376497741811395	17.159383949051847
64-65	12.533388416801408	54.800453014039164	15.775715313441555	16.890443255717877
66-67	12.874567190550149	55.108220173551246	15.12018922500392	16.897023410894686
68-69	13.269710134605797	54.8201695035966	14.808062103838756	17.102058257958834
70-71	13.613053945100498	51.680175496075556	15.478411988433214	19.228358570390736
72-73	14.775293304744878	44.24823518517199	16.945784033679292	24.030687476403838
74-75	15.709961180953739	41.366857794080985	17.727127034438546	25.19605399052673
76	15.135758853528591	42.09521638793128	17.494800421664436	25.274224336875694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.5
16	13.5
17	26.0
18	19.0
19	21.5
20	36.0
21	41.0
22	65.0
23	98.0
24	112.0
25	117.0
26	144.5
27	195.5
28	287.0
29	355.0
30	392.0
31	488.0
32	659.0
33	771.0
34	861.0
35	968.0
36	1129.5
37	1274.0
38	1351.0
39	4597.5
40	9222.5
41	9704.5
42	8731.0
43	7301.0
44	4765.5
45	3275.5
46	2891.0
47	2702.0
48	2374.0
49	2247.5
50	2260.0
51	2115.0
52	1808.5
53	1504.0
54	1361.0
55	1402.5
56	1375.0
57	1206.0
58	1106.0
59	1023.5
60	916.0
61	823.0
62	755.0
63	691.5
64	627.0
65	567.0
66	513.5
67	519.0
68	444.0
69	364.0
70	313.0
71	267.0
72	272.5
73	253.0
74	216.5
75	205.0
76	155.5
77	102.0
78	103.5
79	109.0
80	105.0
81	85.0
82	71.0
83	73.0
84	52.0
85	24.0
86	13.0
87	9.0
88	9.5
89	6.0
90	4.0
91	4.0
92	2.0
93	1.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03988035892323031
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.121492664862555E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.121492664862555E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.011394388263780088
52-53	0.02919811992593648
54-55	0.04557755305512035
56-57	0.04557755305512035
58-59	0.034183164791340266
60-61	0.03916820965674405
62-63	0.02991026919242273
64-65	0.0192280301951289
66-67	0.04130465745620282
68-69	0.007121492664862555
70-71	0.0128186867967526
72-73	0.026349522859991453
74-75	0.01780373166215639
76	0.017091582395670133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	70210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.984902435550488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.449901337426006	11.290414470873095
2	20.264196448147338	10.531263352798746
3	14.56917342688007	11.357356501922803
4	9.471607103705328	9.844751459905996
5	5.6840605130453845	7.384987893462469
6	2.800920850690638	4.366899302093719
7	1.5621574216180663	2.8414755732801593
8	0.8112256084192063	1.686369463039453
9	0.40561280420960316	0.9485828229596923
>10	0.8879631659723745	3.55647343683236
>50	0.027406270554702915	0.5013530836063239
>100	0.04933128699846524	3.038028770830366
>500	0.0	0.0
>1k	0.010962508221881167	5.627403503774391
>5k	0.0	0.0
>10k+	0.005481254110940584	27.024640364620424
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	18974	27.024640364620424	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	2457	3.4995014955134596	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1494	2.1279020082609312	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	430	0.6124483691781798	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	375	0.5341119498646917	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	316	0.4500783364193135	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	313	0.44580544082039597	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	164	0.23358495940749183	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	153	0.2179176755447942	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	133	0.18943170488534397	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	127	0.18088591368750892	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	122	0.17376442102264633	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG	92	0.13103546503347102	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	81	0.1153681811707734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.007121492664862555	0.0
5	0.0	0.0	0.0	0.011394388263780088	0.0
6	0.0	0.0	0.0	0.011394388263780088	0.0
7	0.0	0.0	0.0	0.011394388263780088	0.0
8	0.0	0.0	0.0	0.011394388263780088	0.0
9	0.0	0.0	0.0	0.011394388263780088	0.0
10	0.0	0.0	0.0	0.015667283862697622	0.0
11	0.0	0.0	0.0	0.018515880928642644	0.0
12	0.0	0.0	0.0	0.018515880928642644	0.0
13	0.0	0.0	0.0	0.018515880928642644	0.0
14	0.0	0.0	0.0	0.018515880928642644	0.0
15	0.0	0.0	0.0	0.019940179461615155	0.0
16	0.0	0.0	0.0	0.019940179461615155	0.0
17	0.0	0.0	0.0	0.019940179461615155	0.0
18	0.0	0.0	0.0	0.019940179461615155	0.0
19	0.0	0.0	0.0	0.02706167212647771	0.0
20	0.0	0.0	0.0	0.034183164791340266	0.0
21	0.0	0.0	0.0	0.03560746332431278	0.0
22	0.0	0.0	0.0	0.04130465745620282	0.0
23	0.0	0.0	0.0	0.04415325452214784	0.0
24	0.0	0.0	0.0	0.04415325452214784	0.0
25	0.0	0.0	0.0	0.04700185158809286	0.0
26	0.0	0.0	0.0	0.05269904571998291	0.0
27	0.0	0.0	0.0	0.06266913545079049	0.0
28	0.0	0.0	0.0	0.1167924797037459	0.0
29	0.0	0.0	0.0	0.16237003275886625	0.0
30	0.0	0.0	0.0	0.20225039168209658	0.0
31	0.0	0.0	0.0	0.24213075060532688	0.0
32	0.0	0.0	0.0	0.26634382566585957	0.0
33	0.0	0.0	0.0	0.3076484831220624	0.0
34	0.0	0.0	0.0	0.3916820965674405	0.0
35	0.0	0.0	0.0	0.509898874804159	0.0
36	0.0	0.0	0.0	0.7135735650192281	0.0
37	0.0	0.0	0.0	1.2035322603617717	0.0
38	0.0	0.0	0.0	1.5795470730665147	0.0
39	0.0	0.0	0.0	1.9797749608317903	0.0
40	0.0	0.0	0.0	2.4398233869819115	0.0
41	0.0	0.0	0.0	2.867112946873665	0.0
42	0.0	0.0	0.0	3.369890329012961	0.0
43	0.0	0.0	0.0	3.9510041304657455	0.0
44	0.0	0.0	0.0	4.609030052699046	0.0
45	0.0	0.0	0.0	5.251388691069648	0.0
46	0.0	0.0	0.0	5.888050135308361	0.0
47	0.0	0.0	0.0	6.447799458766558	0.0
48	0.0	0.0	0.0	6.981911408631249	0.0
49	0.0	0.0	0.0	7.39068508759436	0.0
50	0.0	0.0	0.0	7.819398946019086	0.0
51	0.0	0.0	0.0	8.23671841618003	0.0
52	0.0	0.0	0.0	8.749465888050135	0.0
53	0.0	0.0	0.0	9.238000284859707	0.0
54	0.0	0.0	0.0	9.774960831790343	0.0
55	0.0	0.0	0.0	10.217917675544793	0.0
56	0.0	0.0	0.0	10.562597920524142	0.0
57	0.0	0.0	0.0	11.002706167212647	0.0
58	0.0	0.0	0.0	11.38441817404928	0.0
59	0.0	0.0	0.0	11.820253525138869	0.0
60	0.0	0.0	0.0	12.226178607036035	0.0
61	0.0	0.0	0.0	12.595071927075915	0.0
62	0.0	0.0	0.0	12.968238142714712	0.0
63	0.0	0.0	0.0	13.288705312633528	0.0
64	0.0	0.0	0.0	13.707449081327447	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGAAA	15	0.0022062627	70.07918	57
TCGAAAA	15	0.0022062627	70.07918	58
TAAGAGA	30	8.313287E-6	58.316124	4
GAGCTCG	25	2.379018E-4	55.98348	36
GCTCGTA	25	2.379018E-4	55.98348	38
CAGAGCT	25	2.379018E-4	55.98348	34
AGAGCTC	25	2.379018E-4	55.98348	35
CGTCTTC	2750	0.0	52.675358	48
GTCTTCT	2750	0.0	52.58558	49
TGCTCGA	20	0.0068940856	52.55938	55
AGCAATT	20	0.006913572	52.521915	67
TGGAGAT	20	0.006913572	52.521915	67
CAGATAA	20	0.006913572	52.521915	59
AAGCAAT	20	0.006913572	52.521915	66
AAACACG	20	0.006913572	52.521915	69
CCGTCTT	2765	0.0	52.516144	47
CTTAATA	20	0.0069330996	52.48451	1
TTTAGTG	20	0.0069330996	52.48451	23
CCTTATA	40	7.3982847E-7	52.48451	1
TGAAGGC	20	0.0069330996	52.48451	21
>>END_MODULE