FastQCFastQC Report
Wed 25 May 2016
SRR1779798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149714
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTATA150.002223176469.9836351
TAGATCC200.006928369452.5228139
CGAGATT200.006937535552.50526441
ACGAACT200.006937535552.50526424
GGCCCTT200.00694671152.4877241
ATACTCG200.00694671152.4877245
CAGTCGA200.00694671152.48772412
TCGACAG200.00694671152.48772445
GTAGATC1100.050.9312138
CTTCCGA552.2682798E-950.89718618
GTGGACG352.0774676E-550.0050148
TAGATCT1250.047.6206839
CGTGCTT305.8697024E-446.65575810
CGCCGTA451.6786125E-646.65575853
ATCTCGG950.044.21496242
CGACGAT659.946234E-943.0668522
TATCCAC659.946234E-943.066853
TTCCGAC757.4942363E-1041.9901819
GATCTCG1100.041.36778341
AGATCTC1450.041.03859340