##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779798_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149714
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.912793726705587	34.0	23.0	34.0	12.0	34.0
2	29.54053061169964	34.0	31.0	34.0	12.0	34.0
3	29.28047477189842	34.0	27.0	34.0	11.0	34.0
4	29.359171486968485	34.0	27.0	34.0	12.0	34.0
5	30.0500554390371	34.0	32.0	34.0	11.0	34.0
6	32.527305395620985	37.0	31.0	38.0	12.0	38.0
7	32.868475894037964	38.0	34.0	38.0	12.0	38.0
8	32.159183509892195	37.0	31.0	38.0	11.0	38.0
9	33.01912980749963	38.0	34.0	38.0	12.0	38.0
10-11	31.648680150152963	37.0	28.5	38.0	11.5	38.0
12-13	32.45821366071309	37.0	31.0	38.0	11.0	38.0
14-15	31.2970463684091	37.0	27.0	38.0	11.0	38.0
16-17	31.88653031780595	37.0	28.5	38.0	11.0	38.0
18-19	32.68144929665897	37.5	32.0	38.0	11.0	38.0
20-21	31.48960685039475	37.0	27.5	38.0	11.0	38.0
22-23	32.30442042828326	37.0	31.0	38.0	11.0	38.0
24-25	32.599917175414454	37.5	32.0	38.0	11.0	38.0
26-27	31.543606476348238	37.0	26.5	38.0	11.0	38.0
28-29	32.66230947005624	37.5	32.5	38.0	11.0	38.0
30-31	33.029806831692426	38.0	34.0	38.0	11.0	38.0
32-33	33.168621505002875	38.0	34.0	38.0	11.0	38.0
34-35	33.11349306010126	38.0	34.0	38.0	11.0	38.0
36-37	32.62513191819069	38.0	32.5	38.0	11.0	38.0
38-39	32.57161988858758	38.0	32.5	38.0	11.0	38.0
40-41	32.53374433920675	37.5	32.0	38.0	11.0	38.0
42-43	32.89680991757618	38.0	33.0	38.0	11.0	38.0
44-45	32.95767596884727	38.0	34.0	38.0	11.0	38.0
46-47	32.88485712758994	38.0	34.0	38.0	11.0	38.0
48-49	32.62026931349106	38.0	32.5	38.0	11.0	38.0
50-51	32.04495237586331	37.5	29.5	38.0	11.0	38.0
52-53	32.554670905860505	37.0	32.0	38.0	11.0	38.0
54-55	32.67881427254632	37.5	32.5	38.0	11.0	38.0
56-57	32.72161254124531	38.0	33.0	38.0	11.0	38.0
58-59	32.912446397798476	38.0	34.0	38.0	11.0	38.0
60-61	32.85406508409367	38.0	34.0	38.0	11.0	38.0
62-63	32.8189815247739	38.0	34.0	38.0	11.0	38.0
64-65	32.82795530144141	38.0	33.5	38.0	11.0	38.0
66-67	32.72513258613088	38.0	32.5	38.0	11.0	38.0
68-69	32.14984904551345	37.0	31.0	38.0	11.0	38.0
70-71	32.257243811533996	37.0	31.0	38.0	11.0	38.0
72-73	32.44739636907704	37.0	31.0	38.0	11.0	38.0
74-75	32.51266080660459	37.5	31.5	38.0	11.0	38.0
76	31.560121297941407	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	49.0
13	463.0
14	1554.0
15	2602.0
16	3403.0
17	3783.0
18	3858.0
19	3442.0
20	2646.0
21	2107.0
22	1546.0
23	1420.0
24	1313.0
25	1408.0
26	1449.0
27	1715.0
28	1928.0
29	2386.0
30	2857.0
31	3704.0
32	4761.0
33	6313.0
34	8560.0
35	13344.0
36	23564.0
37	49537.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.81715804801154	17.24354435790908	9.405933980790039	32.53336361328934
2	24.649665361956796	20.945268979520954	28.180397290834524	26.224668367687727
3	18.08314519684198	23.208250397424422	30.178206446958868	28.530397958774728
4	15.91434334798349	21.45089971545747	30.243664587146156	32.39109234941288
5	14.969875896709727	31.299678052820713	34.163137715911674	19.56730833455789
6	28.42171354524991	36.96606173144617	17.549578192942498	17.062646530361427
7	27.132513058928836	31.127676913416963	18.456842077137857	23.282967950516344
8	23.992037833649505	35.51580409864536	19.374382130547467	21.117775937157667
9	24.599198396793586	18.810287241148966	18.59251837007348	37.99799599198397
10-11	23.139993520828533	26.107053279496096	24.19202393953664	26.56092926013873
12-13	23.784014854990183	22.637829461506605	25.341651415365295	28.236504268137917
14-15	25.0457539041105	24.28330015896977	21.989593491590632	28.681352445329093
16-17	25.744753329681924	25.91708190282806	22.281817331712464	26.05634743577755
18-19	22.444133471823765	25.648004969624505	24.73356734208584	27.17429421646589
20-21	24.23353861362331	24.747852572237733	24.526096423848138	26.492512390290823
22-23	25.720383135620388	24.861400555733677	23.28437266217805	26.133843646467884
24-25	22.79570898014802	27.55196783071045	22.951345285489086	26.700977903652444
26-27	23.932965520926565	27.65873598995418	23.890217347743032	24.518081141376225
28-29	22.740055442369993	27.067232223372635	25.229618249223474	24.963094085033898
30-31	23.449710781890808	26.21030765325888	25.0554390370974	25.28454252775292
32-33	21.90109141429659	27.599289311620822	24.930200248473756	25.569419025608823
34-35	22.182033144299943	28.35166890434109	24.34890353953336	25.11739441182561
36-37	24.163663147065012	25.762682424149304	24.28859174736364	25.78506268142205
38-39	24.36206597106251	25.2084140492445	25.819962325152634	24.609557654540353
40-41	22.917772946327485	26.48892718425526	25.119645188977614	25.47365468043964
42-43	21.18439157326636	26.137836140908664	26.24737833469148	26.430393951133496
44-45	20.782066183052113	24.651316580497777	27.538308417832287	27.028308818617823
46-47	21.413327722504434	25.701604095905097	26.59900274195865	26.286065439631823
48-49	21.271891740251412	26.28044137488812	26.285450926433068	26.162215958427403
50-51	23.51748690819707	25.709361974991985	25.079819920914826	25.693331195896118
52-53	19.516814301174396	27.003086296043932	26.21982176974361	27.260277633038065
54-55	20.31349155984262	25.799426857536023	25.986800355375046	27.90028122724631
56-57	20.796285781088216	26.488192658405424	25.382277297170912	27.333244263335448
58-59	20.079492317969272	26.56546426185705	25.654308617234467	27.700734802939213
60-61	19.593400609965894	25.63259743253129	26.098589996692954	28.67541196080986
62-63	20.35496950628912	25.993133070145557	25.589333547089904	28.062563876475423
64-65	20.98608721794465	25.567785145224057	24.908055383895913	28.538072252935383
66-67	19.89117473169461	27.951192301397228	23.55776457266542	28.59986839424275
68-69	20.539161334277356	28.989272880291754	23.131437273735255	27.34012851169563
70-71	20.191164486955127	29.31255593999225	23.699854389035092	26.796425184017526
72-73	20.89608069068016	29.042967119215806	23.09971110331814	26.961241086785897
74-75	21.458654099855384	29.315694375327723	22.844365475112973	26.381286049703924
76	20.974550798209872	30.448867811101465	22.26504575512658	26.311535635562088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	2.0
13	3.0
14	3.5
15	4.0
16	10.5
17	16.0
18	23.0
19	39.5
20	63.5
21	78.0
22	107.0
23	151.5
24	203.5
25	240.0
26	324.0
27	507.5
28	707.5
29	808.0
30	913.0
31	1204.0
32	1683.0
33	1976.0
34	2229.0
35	2828.0
36	3390.0
37	3606.0
38	4033.0
39	5180.5
40	6196.0
41	6910.0
42	7329.0
43	7662.0
44	8372.0
45	8906.0
46	9063.0
47	9178.5
48	8993.0
49	8543.5
50	8395.0
51	7956.5
52	7049.5
53	6264.5
54	5948.0
55	5674.0
56	4925.5
57	4149.0
58	3847.0
59	3483.0
60	2890.0
61	2503.0
62	2345.0
63	2162.0
64	1852.5
65	1622.0
66	1467.5
67	1417.0
68	1299.5
69	1171.0
70	1050.5
71	941.0
72	929.5
73	844.5
74	759.0
75	747.0
76	670.5
77	554.5
78	475.5
79	436.0
80	379.0
81	300.0
82	247.0
83	216.0
84	175.5
85	126.0
86	102.5
87	88.0
88	75.5
89	53.0
90	36.5
91	24.0
92	18.0
93	18.0
94	15.0
95	9.5
96	7.0
97	7.5
98	6.5
99	8.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	6.679402059927595E-4
7	0.005343521647942076
8	0.004007641235956557
9	0.009351162883898633
10-11	0.002337790720974658
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0030057309269674176
20-21	0.0
22-23	0.001335880411985519
24-25	0.004007641235956557
26-27	0.0
28-29	0.006011461853934835
30-31	0.0
32-33	0.0
34-35	0.003339701029963798
36-37	0.019036295870793647
38-39	0.008015282471913115
40-41	3.3397010299637977E-4
42-43	0.0
44-45	0.005343521647942076
46-47	0.002337790720974658
48-49	0.0
50-51	0.001335880411985519
52-53	0.013358804119855192
54-55	0.008683222677905873
56-57	0.01269086391386243
58-59	0.009351162883898633
60-61	0.022375996900757446
62-63	0.007347342265920356
64-65	0.021040116488771927
66-67	0.016364535046822608
68-69	0.0
70-71	0.0
72-73	0.0043416113389529365
74-75	0.005009551544945696
76	0.002671760823971038
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	149714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3777258082176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2129996760883	54.04569498430125
2	8.881999964133199	11.968979164239304
3	4.819999980536142	9.742819112525352
4	2.6220003892523835	7.066576931843477
5	1.304999998659547	4.396396604470365
6	0.7449999970358586	3.0117843316443005
7	0.39599999840135913	1.8677105518638968
8	0.26099999894605025	1.4068469091945646
9	0.1809999992690959	1.0975831489836656
>10	0.5749999976780668	5.395608260933819
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01269086391386243	0.0
2	0.0	0.0	0.0	0.01269086391386243	0.0
3	0.0	0.0	0.0	0.01269086391386243	0.0
4	0.0	0.0	0.0	0.019370265973790025	0.0
5	0.0	0.0	0.0	0.022042026797761065	0.0
6	0.0	0.0	0.0	0.022042026797761065	0.0
7	0.0	0.0	0.0	0.022709967003753825	0.0
8	0.0	0.0	0.0	0.022709967003753825	0.0
9	0.0	0.0	0.0	0.024713787621732104	0.0
10	0.0	0.0	0.0	0.02538172782772486	0.0
11	0.0	0.0	0.0	0.02538172782772486	0.0
12	0.0	0.0	0.0	0.02538172782772486	0.0
13	0.0	0.0	0.0	0.02538172782772486	0.0
14	0.0	0.0	0.0	0.02671760823971038	0.0
15	0.0	0.0	0.0	0.0280534886516959	0.0
16	0.0	0.0	0.0	0.0280534886516959	0.0
17	0.0	0.0	0.0	0.02872142885768866	0.0
18	0.0	0.0	0.0	0.032729070093645216	0.0
19	0.0	0.0	0.0	0.032729070093645216	0.0
20	0.0	0.0	0.0	0.036736711329601775	0.0
21	0.0	0.0	0.0	0.039408472153572814	0.0
22	0.0	0.0	0.0	0.05209933606743524	0.0
23	0.0	0.0	0.0	0.054103156685413524	0.0
24	0.0	0.0	0.0	0.05877873812736284	0.0
25	0.0	0.0	0.0	0.06479019998129767	0.0
26	0.0	0.0	0.0	0.06479019998129767	0.0
27	0.0	0.0	0.0	0.09351162883898634	0.0
28	0.0	0.0	0.0	0.11421777522476188	0.0
29	0.0	0.0	0.0	0.14761478552439986	0.0
30	0.0	0.0	0.0	0.17700415458808128	0.0
31	0.0	0.0	0.0	0.21708056694764685	0.0
32	0.0	0.0	0.0	0.25314933807125584	0.0
33	0.0	0.0	0.0	0.28454252775291555	0.0
34	0.0	0.0	0.0	0.34398920608627115	0.0
35	0.0	0.0	0.0	0.43816877513125024	0.0
36	0.0	0.0	0.0	0.5597338926219325	0.0
37	0.0	0.0	0.0	0.8322534966669783	0.0
38	0.0	0.0	0.0	1.1849259254311555	0.0
39	0.0	0.0	0.0	1.5656518428470283	0.0
40	0.0	0.0	0.0	1.9730953685026116	0.0
41	0.0	0.0	0.0	2.5622186301882257	0.0
42	0.0	0.0	0.0	3.1967618258813473	0.0
43	0.0	0.0	0.0	3.7685186422111494	0.0
44	0.0	0.0	0.0	4.3836915719304805	0.0
45	0.0	0.0	0.0	4.99352098000187	0.0
46	0.0	0.0	0.0	5.640087099402861	0.0
47	0.0	0.0	0.0	6.289992919833817	0.0
48	0.0	0.0	0.0	6.820337443392067	0.0
49	0.0	0.0	0.0	7.43818213393537	0.0
50	0.0	0.0	0.0	8.022629814179036	0.0
51	0.0	0.0	0.0	8.601066032568765	0.0
52	0.0	0.0	0.0	9.308414710715097	0.0
53	0.0	0.0	0.0	10.053168040397024	0.0
54	0.0	0.0	0.0	10.650306584554551	0.0
55	0.0	0.0	0.0	11.318914730753303	0.0
56	0.0	0.0	0.0	11.890003606877112	0.0
57	0.0	0.0	0.0	12.415672548993415	0.0
58	0.0	0.0	0.0	12.90126507875015	0.0
59	0.0	0.0	0.0	13.46300279199006	0.0
60	0.0	0.0	0.0	13.99735495678427	0.0
61	0.0	0.0	0.0	14.533710942196455	0.0
62	0.0	0.0	0.0	15.028654634837089	0.0
63	0.0	0.0	0.0	15.605754972814834	0.0
64	0.0	0.0	0.0	16.111385708751353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTATA	15	0.0022231764	69.983635	1
TAGATCC	20	0.0069283694	52.52281	39
CGAGATT	20	0.0069375355	52.505264	41
ACGAACT	20	0.0069375355	52.505264	24
GGCCCTT	20	0.006946711	52.487724	1
ATACTCG	20	0.006946711	52.487724	5
CAGTCGA	20	0.006946711	52.487724	12
TCGACAG	20	0.006946711	52.487724	45
GTAGATC	110	0.0	50.93121	38
CTTCCGA	55	2.2682798E-9	50.897186	18
GTGGACG	35	2.0774676E-5	50.00501	48
TAGATCT	125	0.0	47.62068	39
CGTGCTT	30	5.8697024E-4	46.655758	10
CGCCGTA	45	1.6786125E-6	46.655758	53
ATCTCGG	95	0.0	44.214962	42
CGACGAT	65	9.946234E-9	43.06685	22
TATCCAC	65	9.946234E-9	43.06685	3
TTCCGAC	75	7.4942363E-10	41.99018	19
GATCTCG	110	0.0	41.367783	41
AGATCTC	145	0.0	41.038593	40
>>END_MODULE