FastQCFastQC Report
Wed 25 May 2016
SRR1779798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149714
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1714011.448495130715898No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG22421.497521941835767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC13020.8696581482025728No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4450.297233391666778RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3490.2331111318914731No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT3080.2057255834457699No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT2630.17566827417609576No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1880.12557275872663878No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1610.10753837316483429RNA PCR Primer, Index 32 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTC150.002224018369.9769513
GGTCTAT150.002224018369.976951
ATTTGCA252.3881842E-455.98156443
GCACGTA200.00691267452.55292552
ATTTGTG200.00693098652.51779660
CCGAGTG200.00694933452.4827164
CGTCCAA200.00694933452.48271622
ATCTCGT23050.052.3688737
CTCGTAT23750.052.2985739
CGTCTTC23950.052.0426948
GCCGTCT24100.052.00932746
CGTATGC24250.051.94165441
TCGTATG24250.051.94165440
TCTCGTA23250.051.91838538
GATCTCG23100.051.8011236
CCGTCTT24200.051.7944147
TATGCCG24200.051.7598143
GTCTTCT24150.051.7566749
CTGCTTG24200.051.6842854
ATGCCGT24200.051.6152344