FastQCFastQC Report
Wed 25 May 2016
SRR1779797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65141
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT301.00679244E-770.02383437
GCCGTAT150.002212466270.02383454
AGATCTA207.889363E-569.9700540
ACATTTG207.889363E-569.970058
CTAGGCG150.00221922669.9700544
TAGTCCA150.00221922669.9700520
GATCTCG950.062.60477441
TATGACG401.0499207E-861.2237911
GACGATC308.275218E-658.35319523
GACGAAA601.8189894E-1258.35319523
TCTTACG308.31306E-658.30837611
TCGGCGG308.31306E-658.30837645
TTGCCGA308.31306E-658.30837618
CATTTGA308.31306E-658.3083769
TGGTCGC601.8189894E-1258.30837649
GGTGGTC553.45608E-1157.24822247
GTCGATC252.3702598E-456.01906638
ACGTGTA252.3702598E-456.01906634
GTCATCG252.3792885E-455.976044
CTGACGT252.3792885E-455.9760412
Produced by FastQC (version 0.11.5)