##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779797_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65141
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.418415437282203	23.0	12.0	34.0	12.0	34.0
2	23.459817933405997	23.0	12.0	34.0	12.0	34.0
3	23.393208578314734	23.0	11.0	34.0	11.0	34.0
4	24.34500544971677	23.0	12.0	34.0	12.0	34.0
5	23.663637340538216	23.0	11.0	34.0	11.0	34.0
6	25.413395557329487	27.0	12.0	37.0	11.0	38.0
7	26.073686311232557	27.0	12.0	37.0	11.0	38.0
8	25.2193856403801	27.0	11.0	37.0	11.0	38.0
9	26.158241353371917	28.0	12.0	37.0	11.0	38.0
10-11	25.250602539107476	26.0	11.5	37.0	11.0	38.0
12-13	25.45581891589015	27.0	11.0	37.0	11.0	38.0
14-15	24.669087057306456	26.0	11.0	36.5	11.0	38.0
16-17	24.77145730031777	26.0	11.0	37.0	11.0	38.0
18-19	24.881464822462043	26.0	11.0	37.0	11.0	38.0
20-21	24.68229686372638	25.5	11.0	36.5	11.0	38.0
22-23	24.928117468261156	26.0	11.0	37.0	11.0	38.0
24-25	25.185697179963462	26.0	11.0	37.0	11.0	38.0
26-27	24.4814018820712	25.5	11.0	37.0	11.0	38.0
28-29	24.67198845581124	26.0	11.0	37.0	11.0	38.0
30-31	24.764925315853304	26.0	11.0	37.5	11.0	38.0
32-33	24.94977049784314	26.0	11.0	38.0	11.0	38.0
34-35	25.088745951090708	26.0	11.0	37.5	11.0	38.0
36-37	24.784513593589292	26.0	11.0	37.0	11.0	38.0
38-39	24.609585360986166	26.0	11.0	37.0	11.0	38.0
40-41	24.63978139727668	26.0	11.0	37.0	11.0	38.0
42-43	24.847361876544724	26.0	11.0	37.0	11.0	38.0
44-45	24.98793386653567	26.0	11.0	37.0	11.0	38.0
46-47	24.85843785020187	26.0	11.0	37.0	11.0	38.0
48-49	24.552263551373173	26.0	11.0	37.0	11.0	38.0
50-51	24.24188299227829	25.0	11.0	37.0	11.0	38.0
52-53	24.647073271825732	26.0	11.0	37.0	11.0	38.0
54-55	24.64814786386454	25.5	11.0	37.0	11.0	38.0
56-57	24.656836708064045	25.5	11.0	37.0	11.0	38.0
58-59	24.515635314164655	25.0	11.0	37.0	11.0	38.0
60-61	24.71552478469781	25.0	11.0	37.0	11.0	38.0
62-63	24.532421976942324	25.0	11.0	37.0	11.0	38.0
64-65	24.523057674889856	25.0	11.0	37.0	11.0	38.0
66-67	24.80044825839333	25.0	11.0	37.0	11.0	38.0
68-69	24.530656575735712	25.0	11.0	37.0	11.0	38.0
70-71	24.702775517723094	25.0	11.0	37.0	11.0	38.0
72-73	24.491456993291475	25.0	11.0	37.0	11.0	38.0
74-75	24.329945809858614	24.5	11.0	37.0	11.0	38.0
76	23.88122687708202	24.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	56.0
13	594.0
14	2313.0
15	4033.0
16	5341.0
17	5970.0
18	5963.0
19	5242.0
20	3942.0
21	2770.0
22	1898.0
23	1354.0
24	1033.0
25	780.0
26	635.0
27	603.0
28	578.0
29	601.0
30	650.0
31	739.0
32	917.0
33	1145.0
34	1628.0
35	2283.0
36	4353.0
37	9720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.33734514361155	20.400362291030227	17.024608157688707	37.23768440766952
2	12.953439462089927	34.046146052409384	28.763758615925454	24.23665586957523
3	10.990006294039084	33.33691530679603	32.197847745659416	23.47523065350547
4	9.841727943998404	33.86653566878003	20.724275034156676	35.567461353064886
5	11.240232725932977	33.161910317618705	33.834297907615785	21.76355904883253
6	16.87928890526413	41.277882681650006	20.829303489460997	21.013524923624864
7	16.273642839597148	36.35316875460575	15.716347334807173	31.656841070989927
8	14.682448302860038	41.81058966210718	20.23058382842844	23.27637820660434
9	18.014339008551207	22.022813454718516	16.864454918095706	43.09839261863457
10-11	15.75335631001635	33.461010001765466	22.60721385969895	28.17841982851923
12-13	15.936199935524478	24.80925991311156	25.77332248507085	33.48121766629312
14-15	25.96828418354032	28.866612425354234	18.399318401621098	26.76578498948435
16-17	26.84484426091095	25.714987488678403	17.818271134922707	29.621897115487943
18-19	18.661488037212447	25.86756115720876	22.67748447562539	32.79346632995341
20-21	25.889992477855728	24.546752429345574	19.036397967485915	30.526857125312784
22-23	27.039661035163	24.44983458577361	17.976803629134398	30.533700749929
24-25	18.57335851026267	32.241821335912434	17.345983205145917	31.838836948678978
26-27	23.97433240457166	31.94172596157536	18.87305132751514	25.21089030633784
28-29	19.46510681743162	30.7049259608963	22.3330185999739	27.49694862169818
30-31	21.277853255655085	28.495329326609404	22.55202216746878	27.674795250266733
32-33	18.041003676668126	31.337647085914295	22.616498184693086	28.004851052724494
34-35	17.356862684894494	34.10118439939514	20.850956039822837	27.69099687588753
36-37	23.665300105946844	26.17040551537765	22.084542509251158	28.079751869424356
38-39	24.2991156420807	23.454676656635755	25.210342074556287	27.03586562672726
40-41	19.46499846484495	29.404359840343876	22.837734111145224	28.29290758366595
42-43	14.844181762357996	28.97988946883635	24.704482652747927	31.47144611605772
44-45	14.538253106841575	24.783346254519216	28.46089486248532	32.217505776153885
46-47	17.35876573533927	27.89146453791833	24.491096100706173	30.25867362603623
48-49	18.95594906394639	28.77395782961445	23.03098686684935	29.23910623958981
50-51	25.550173860313024	27.62498368860198	18.798406472362736	28.026435978722258
52-53	13.418697127416209	31.810295856171216	22.487832588703117	32.283174427709454
54-55	16.646068580113763	27.094288061041983	21.86518872197189	34.39445463687237
56-57	18.783058628445968	29.15070512279193	20.02364483613668	32.04259141262542
58-59	17.5699327540148	29.509165719900516	20.92762612460466	31.993275401480027
60-61	14.525478513900511	27.386427326541114	22.236980506268857	35.85111365328952
62-63	17.09092025670157	28.198022538152117	21.043541007768603	33.66751619737771
64-65	17.46140204064398	27.32144365196963	20.924662003946164	34.29249230344023
66-67	14.929028196801855	34.72052693397204	17.569071800895113	32.78137306833099
68-69	16.088301440731957	38.0746233142208	15.790483646886347	30.046591598160898
70-71	14.511597918361707	39.16273928862007	17.350056032299165	28.975606760719057
72-73	16.07534888005281	38.35838310023489	17.23290909928305	28.333358920429248
74-75	17.91008113730397	37.510458805738715	16.48000736914019	28.099452687817124
76	16.109917101627264	41.28645993245318	15.036843721215842	27.566779244703714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	2.5
14	3.0
15	3.0
16	5.5
17	7.0
18	6.0
19	16.0
20	26.0
21	25.0
22	31.0
23	50.0
24	69.0
25	75.0
26	95.5
27	158.5
28	236.0
29	271.0
30	335.0
31	490.0
32	684.5
33	788.0
34	910.5
35	1195.0
36	1471.0
37	1585.0
38	1823.5
39	2232.0
40	2667.5
41	3067.0
42	3201.0
43	3467.5
44	3891.0
45	4098.0
46	4148.0
47	4155.5
48	4105.5
49	3899.5
50	3751.0
51	3614.5
52	3361.0
53	3046.0
54	2848.0
55	2606.0
56	2210.0
57	1944.0
58	1832.0
59	1602.5
60	1261.0
61	1047.5
62	946.0
63	850.0
64	697.0
65	573.5
66	480.0
67	453.0
68	419.0
69	336.0
70	264.5
71	242.0
72	213.5
73	188.0
74	182.0
75	173.0
76	158.5
77	136.5
78	133.5
79	138.0
80	129.5
81	100.5
82	80.0
83	80.0
84	69.5
85	57.5
86	44.0
87	32.0
88	34.0
89	32.5
90	22.0
91	12.0
92	9.0
93	8.0
94	4.5
95	2.0
96	2.0
97	6.0
98	10.5
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0030702629680232113
7	0.007675657420058028
8	0.0030702629680232113
9	0.006140525936046423
10-11	0.003837828710029014
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.002302697226017408
20-21	0.0
22-23	0.002302697226017408
24-25	0.0030702629680232113
26-27	7.675657420058028E-4
28-29	0.009978354646075436
30-31	7.675657420058028E-4
32-33	7.675657420058028E-4
34-35	0.003837828710029014
36-37	0.02149184077616248
38-39	0.013816183356104449
40-41	0.0015351314840116057
42-43	0.0015351314840116057
44-45	0.003837828710029014
46-47	0.0015351314840116057
48-49	7.675657420058028E-4
50-51	0.003837828710029014
52-53	0.012281051872092845
54-55	0.00844322316206383
56-57	0.016118880582121856
58-59	0.01074592038808124
60-61	0.026864800970203096
62-63	0.01074592038808124
64-65	0.02225940651816828
66-67	0.014583749098110254
68-69	7.675657420058028E-4
70-71	0.0
72-73	0.006140525936046423
74-75	0.0069080916780522245
76	0.0015351314840116057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	65141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.89304738950892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.08870554564245	80.88761302405551
2	3.102309064890554	5.391381771848759
3	1.7119233962864158	4.4626272240217375
4	1.0158472165786265	3.530802413226693
5	0.5476741515467378	2.3794538002179886
6	0.27560376658481	1.4368830690348628
7	0.12366835680087628	0.7522144271656868
8	0.058300796777555966	0.4052747117790639
9	0.03533381622882179	0.276323667122089
>10	0.04063388866314506	0.47742589152760934
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0015351314840116057	0.0
4	0.0	0.0	0.0	0.0015351314840116057	0.0
5	0.0	0.0	0.0	0.01074592038808124	0.0
6	0.0	0.0	0.0	0.01074592038808124	0.0
7	0.0	0.0	0.0	0.01074592038808124	0.0
8	0.0	0.0	0.0	0.012281051872092845	0.0
9	0.0	0.0	0.0	0.012281051872092845	0.0
10	0.0	0.0	0.0	0.012281051872092845	0.0
11	0.0	0.0	0.0	0.01381618335610445	0.0
12	0.0	0.0	0.0	0.015351314840116056	0.0
13	0.0	0.0	0.0	0.015351314840116056	0.0
14	0.0	0.0	0.0	0.015351314840116056	0.0
15	0.0	0.0	0.0	0.015351314840116056	0.0
16	0.0	0.0	0.0	0.015351314840116056	0.0
17	0.0	0.0	0.0	0.015351314840116056	0.0
18	0.0	0.0	0.0	0.01688644632412766	0.0
19	0.0	0.0	0.0	0.019956709292150872	0.0
20	0.0	0.0	0.0	0.029167498196220505	0.0
21	0.0	0.0	0.0	0.050659338972382985	0.0
22	0.0	0.0	0.0	0.08596736310464992	0.0
23	0.0	0.0	0.0	0.10438894091278918	0.0
24	0.0	0.0	0.0	0.11359972981685881	0.0
25	0.0	0.0	0.0	0.11666999278488202	0.0
26	0.0	0.0	0.0	0.11974025575290524	0.0
27	0.0	0.0	0.0	0.12127538723691685	0.0
28	0.0	0.0	0.0	0.1381618335610445	0.0
29	0.0	0.0	0.0	0.15965367433720698	0.0
30	0.0	0.0	0.0	0.19035630401743908	0.0
31	0.0	0.0	0.0	0.22412919666569442	0.0
32	0.0	0.0	0.0	0.23333998556976404	0.0
33	0.0	0.0	0.0	0.26404261524999617	0.0
34	0.0	0.0	0.0	0.3300532690624952	0.0
35	0.0	0.0	0.0	0.3945287913909826	0.0
36	0.0	0.0	0.0	0.5158041786278995	0.0
37	0.0	0.0	0.0	0.6708524585130716	0.0
38	0.0	0.0	0.0	0.9134032329869053	0.0
39	0.0	0.0	0.0	1.2710888687616095	0.0
40	0.0	0.0	0.0	1.7070662102209053	0.0
41	0.0	0.0	0.0	2.101595001611888	0.0
42	0.0	0.0	0.0	2.4807724781627547	0.0
43	0.0	0.0	0.0	2.8753012695537374	0.0
44	0.0	0.0	0.0	3.226846379392395	0.0
45	0.0	0.0	0.0	3.596813067039192	0.0
46	0.0	0.0	0.0	4.048141723338604	0.0
47	0.0	0.0	0.0	4.456486698085691	0.0
48	0.0	0.0	0.0	4.843339832056616	0.0
49	0.0	0.0	0.0	5.170322838151088	0.0
50	0.0	0.0	0.0	5.558711103606024	0.0
51	0.0	0.0	0.0	5.922537265316774	0.0
52	0.0	0.0	0.0	6.304785004835664	0.0
53	0.0	0.0	0.0	6.5872491978938	0.0
54	0.0	0.0	0.0	6.851291813143796	0.0
55	0.0	0.0	0.0	7.17059916181821	0.0
56	0.0	0.0	0.0	7.565127953209192	0.0
57	0.0	0.0	0.0	7.8614083296234325	0.0
58	0.0	0.0	0.0	8.19299673016994	0.0
59	0.0	0.0	0.0	8.487741975100167	0.0
60	0.0	0.0	0.0	8.7963034033865	0.0
61	0.0	0.0	0.0	9.043459572312369	0.0
62	0.0	0.0	0.0	9.29215087272225	0.0
63	0.0	0.0	0.0	9.566939408360327	0.0
64	0.0	0.0	0.0	9.81102531431817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGAT	30	1.00679244E-7	70.023834	37
GCCGTAT	15	0.0022124662	70.023834	54
AGATCTA	20	7.889363E-5	69.97005	40
ACATTTG	20	7.889363E-5	69.97005	8
CTAGGCG	15	0.002219226	69.97005	44
TAGTCCA	15	0.002219226	69.97005	20
GATCTCG	95	0.0	62.604774	41
TATGACG	40	1.0499207E-8	61.22379	11
GACGATC	30	8.275218E-6	58.353195	23
GACGAAA	60	1.8189894E-12	58.353195	23
TCTTACG	30	8.31306E-6	58.308376	11
TCGGCGG	30	8.31306E-6	58.308376	45
TTGCCGA	30	8.31306E-6	58.308376	18
CATTTGA	30	8.31306E-6	58.308376	9
TGGTCGC	60	1.8189894E-12	58.308376	49
GGTGGTC	55	3.45608E-11	57.248222	47
GTCGATC	25	2.3702598E-4	56.019066	38
ACGTGTA	25	2.3702598E-4	56.019066	34
GTCATCG	25	2.3792885E-4	55.97604	4
CTGACGT	25	2.3792885E-4	55.97604	12
>>END_MODULE