FastQCFastQC Report
Wed 25 May 2016
SRR1779797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65141
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2830343.448826391980475No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG36975.675381096390906No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC20173.0963602032514084No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT7241.1114351944244025RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6070.9318248107950446No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3480.5342257564360388No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT2820.43290707849127275No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2580.39606392287499426No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1930.2962803764142399No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1830.28092906157412384No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1760.2701831411860426No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1670.2563669578299381No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT1230.1888211725334275No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1220.1872860410494159No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG1160.17807525214534625No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1090.167329331757265No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT1010.15504827988517217No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC990.15197801691714896No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT960.14737262246511412No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC780.11974025575290524No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT770.11820512426889364No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT700.10745920388081238No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCC150.002202059470.1070153
CCGCCTT207.8131896E-570.10747
TGCCGCC207.84307E-570.0530845
GAAAACA150.002208797770.0530860
CTATACA301.0092481E-769.999231
GCTTATA150.002215551469.999231
GTACCCA150.002222320569.94546516
CCCACGT150.002222320569.94546519
AGTACCC150.002222320569.94546515
GATCTCG150.002222320569.94546536
ACCCACG150.002222320569.94546518
CTGCCGT207.903103E-569.94546544
CATCAGT401.0528311E-861.20228628
TCAGTTA352.9480907E-759.9532630
GTCTGCC308.330389E-658.2878942
TCTGCCG308.330389E-658.2878943
CTTGAAC252.3563713E-456.085657
TTGAAAC252.3653611E-456.0424658
TGAAACA252.3653611E-456.0424659
TGAAAAC252.3653611E-456.0424659
Produced by FastQC (version 0.11.5)