FastQCFastQC Report
Wed 25 May 2016
SRR1779796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125260
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATC353.6779966E-970.023
ACGATCT352.9544572E-760.024
CTGTGTA601.8189894E-1258.33333634
ATCTCGG1050.056.66666442
TAGATCT1250.056.039
TCGGTGG750.056.045
AGCCGTA252.3827922E-455.99999653
GATCTCG1000.055.99999641
CGATCTA252.3827922E-455.99999625
CGACGAT950.055.2631622
CAGCCGT200.006937656652.512
CCGGACC200.006937656652.553
AACGGAC200.006937656652.535
ACGTGGG200.006937656652.514
TTACGTG200.006937656652.512
CGGTATA200.006937656652.567
CGACTAT200.006937656652.522
CGTTTTC200.006937656652.540
GCCGTAA200.006937656652.554
GGTAGCC200.006937656652.550
Produced by FastQC (version 0.11.5)