##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779796_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125260
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.120557240938847	34.0	23.0	34.0	12.0	34.0
2	28.714873064026825	34.0	23.0	34.0	12.0	34.0
3	28.416525626696473	34.0	23.0	34.0	11.0	34.0
4	28.681542391825005	34.0	23.0	34.0	12.0	34.0
5	28.74461120868593	34.0	23.0	34.0	11.0	34.0
6	31.415711320453457	37.0	27.0	38.0	12.0	38.0
7	31.722696790675396	37.0	27.0	38.0	12.0	38.0
8	31.0942599393262	37.0	26.0	38.0	11.0	38.0
9	31.836867315982754	37.0	28.0	38.0	12.0	38.0
10-11	30.51826201500878	36.5	25.0	38.0	11.0	38.0
12-13	31.225159667890786	37.0	26.5	38.0	11.0	38.0
14-15	30.13845601149609	36.5	24.5	38.0	11.0	38.0
16-17	30.66591090531694	36.5	25.0	38.0	11.0	38.0
18-19	31.343174197668848	37.0	27.0	38.0	11.0	38.0
20-21	30.325307360689767	36.5	24.0	38.0	11.0	38.0
22-23	31.069120229921765	37.0	25.5	38.0	11.0	38.0
24-25	31.353428867954655	37.0	26.5	38.0	11.0	38.0
26-27	30.338799297461282	37.0	23.5	38.0	11.0	38.0
28-29	31.312218585342485	37.0	26.5	38.0	11.0	38.0
30-31	31.63443238064825	38.0	27.0	38.0	11.0	38.0
32-33	31.760729682260894	38.0	27.0	38.0	11.0	38.0
34-35	31.72983394539358	38.0	27.0	38.0	11.0	38.0
36-37	31.303544627175476	37.0	26.5	38.0	11.0	38.0
38-39	31.230372824524988	37.0	26.5	38.0	11.0	38.0
40-41	31.225626696471338	37.0	26.0	38.0	11.0	38.0
42-43	31.54053967747086	37.0	26.5	38.0	11.0	38.0
44-45	31.617978604502635	37.0	27.0	38.0	11.0	38.0
46-47	31.51715631486508	37.0	26.5	38.0	11.0	38.0
48-49	31.24095481398691	37.0	26.5	38.0	11.0	38.0
50-51	30.705684176912023	37.0	24.5	38.0	11.0	38.0
52-53	31.205013571770717	37.0	26.0	38.0	11.0	38.0
54-55	31.310418329873862	37.0	26.5	38.0	11.0	38.0
56-57	31.363356219064347	37.0	26.5	38.0	11.0	38.0
58-59	31.51277742296024	37.0	26.5	38.0	11.0	38.0
60-61	31.465471818617274	37.0	26.5	38.0	11.0	38.0
62-63	31.438112725530896	37.0	27.0	38.0	11.0	38.0
64-65	31.406143222098038	37.0	26.5	38.0	11.0	38.0
66-67	31.400231518441643	37.0	26.5	38.0	11.0	38.0
68-69	30.85845441481718	37.0	24.5	38.0	11.0	38.0
70-71	30.993613284368514	37.0	25.0	38.0	11.0	38.0
72-73	31.129191282133164	37.0	25.0	38.0	11.0	38.0
74-75	31.120369631167172	37.0	25.0	38.0	11.0	38.0
76	30.240866996646975	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	44.0
13	459.0
14	1593.0
15	2969.0
16	3847.0
17	4583.0
18	4656.0
19	4226.0
20	3362.0
21	2535.0
22	1862.0
23	1422.0
24	1314.0
25	1295.0
26	1221.0
27	1448.0
28	1548.0
29	1944.0
30	2251.0
31	2753.0
32	3677.0
33	4474.0
34	6711.0
35	9891.0
36	17933.0
37	37241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.54398850391186	18.94539358135079	9.541753153440844	34.9688647612965
2	19.812390228325082	21.13523870349673	32.710362446112086	26.342008622066103
3	16.749960083027304	20.58198946191921	34.63595720900527	28.032093246048216
4	12.411783490340092	26.785885358454415	28.64282292830912	32.15950822289638
5	16.843365799137793	31.844962478045662	33.28037681622226	18.031294906594283
6	25.04550680206936	37.58861850929297	16.126971961423006	21.238902727214665
7	24.466267465069862	32.359281437125745	17.970459081836328	25.203992015968062
8	21.542109423026258	40.55422052260552	16.316054192580058	21.587615861788166
9	22.727272727272727	19.555913961324116	17.640483528416077	40.07632978298708
10-11	21.815547234885077	27.8842699410013	23.561158258620278	26.739024565493345
12-13	21.931183139070733	23.532252913939004	25.613923040076642	28.92264090691362
14-15	24.431874882742488	25.482502185480428	21.49874060443004	28.586882327347045
16-17	25.758228949616395	25.929474129603463	21.728179212671346	26.5841177081088
18-19	21.70637946245015	25.79289201499359	24.4100963246536	28.090632197902654
20-21	24.375299377295224	25.18880728085582	23.65759220820693	26.778301133642024
22-23	26.07569267862346	24.997505119494797	22.096258477603957	26.830543724277785
24-25	21.789462338979128	28.6023368235327	21.948736782017555	27.659464055470618
26-27	23.86694768439793	28.562817841432555	22.962421861902136	24.60781261226738
28-29	22.103481451023757	27.891528509095842	24.7609770897289	25.244012950151497
30-31	23.046156179770797	27.08816496952327	24.406931210806366	25.45874763989957
32-33	21.442998562988983	28.26041832987386	24.754909787641704	25.54167331949545
34-35	21.228040061793198	29.701053438344516	23.951651211713564	25.119255288148718
36-37	24.089097921474373	25.730438462705518	24.683190532846773	25.497273082973336
38-39	24.211139855003033	24.867857941298585	25.87628948292932	25.04471272076906
40-41	21.851386144542243	26.84110731892302	25.41045446380456	25.897052072730176
42-43	19.66469742934696	26.333226888072808	26.58031294906594	27.42176273351429
44-45	19.557372337367866	24.538131127646665	28.186376265448853	27.718120269536616
46-47	20.39151973366439	25.625621230205457	26.98164950840482	27.00120952772533
48-49	20.505748044068337	26.42064505827878	26.503273191761135	26.57033370589175
50-51	23.39092113876958	26.23425250283415	24.495840585032493	25.878985773363777
52-53	18.087055808178597	27.860573372244822	25.971423210357592	28.08094760921899
54-55	18.961105322618636	26.159195519307616	25.711285963504555	29.168413194569197
56-57	19.750883286424337	27.227578497714433	24.849392179172405	28.172146036688822
58-59	19.391735167656087	27.16072547837578	25.301118239561166	28.146421114406966
60-61	18.39006568308411	25.83161971690391	25.693465631176505	30.08484896883547
62-63	19.67527905082797	26.10622295322805	24.963671494498826	29.25482650144516
64-65	20.204907887276704	25.589130139666366	24.437222004839214	29.768739968217716
66-67	19.25418721178608	28.562074541353084	22.782424690076457	29.40131355678438
68-69	19.839292984935213	30.12078972369251	21.886251686505563	28.153665604866717
70-71	19.366594948886114	30.92939058514524	22.324055261277586	27.379959204691062
72-73	20.154645759816052	30.939131684417266	21.89031887205199	27.015903683714694
74-75	20.92879355840589	30.92172760552023	21.608242813287184	26.541236022786702
76	20.30817133048581	32.62145223743563	20.85745080036725	26.212925631711308
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.5
11	1.5
12	1.0
13	2.0
14	4.0
15	3.0
16	2.5
17	4.0
18	11.0
19	29.0
20	42.0
21	44.0
22	74.5
23	121.0
24	198.0
25	259.0
26	324.5
27	451.5
28	619.0
29	725.0
30	869.0
31	1155.0
32	1450.0
33	1603.0
34	1874.5
35	2401.0
36	2891.0
37	3126.0
38	3579.5
39	4515.5
40	5335.5
41	6029.0
42	6385.0
43	6564.0
44	7079.5
45	7391.5
46	7367.0
47	7437.5
48	7489.0
49	7197.0
50	6924.0
51	6645.0
52	6081.5
53	5364.5
54	4932.0
55	4557.5
56	3961.0
57	3417.0
58	3095.0
59	2930.0
60	2521.0
61	2055.5
62	1834.0
63	1710.0
64	1546.0
65	1366.0
66	1129.0
67	1032.0
68	1003.0
69	893.0
70	742.0
71	672.0
72	646.5
73	579.0
74	530.5
75	524.0
76	457.0
77	379.0
78	338.5
79	309.0
80	292.0
81	240.0
82	181.0
83	157.0
84	142.5
85	115.0
86	83.5
87	65.0
88	64.5
89	49.0
90	22.5
91	15.5
92	20.0
93	17.0
94	12.0
95	9.0
96	8.0
97	5.0
98	3.0
99	6.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003193357815743254
7	0.007983394539358136
8	0.0023950183618074404
9	0.01117675235510139
10-11	0.0023950183618074404
12-13	0.0
14-15	0.0011975091809037202
16-17	7.983394539358135E-4
18-19	0.005189206450582787
20-21	0.0
22-23	0.0027941880887753473
24-25	0.0035925275427111606
26-27	7.983394539358135E-4
28-29	0.008382564266326041
30-31	3.9916972696790677E-4
32-33	0.0
34-35	0.0027941880887753473
36-37	0.021555165256266964
38-39	0.012773431262973017
40-41	0.001995848634839534
42-43	0.0
44-45	0.006386715631486508
46-47	0.0035925275427111606
48-49	0.0
50-51	0.001596678907871627
52-53	0.01476927989781255
54-55	0.009180903720261854
56-57	0.013970940443876737
58-59	0.01476927989781255
60-61	0.029937729522593007
62-63	0.01117675235510139
64-65	0.026345201979881848
66-67	0.02195433498323487
68-69	7.983394539358135E-4
70-71	3.9916972696790677E-4
72-73	0.004790036723614881
74-75	0.009180903720261854
76	0.003991697269679068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	125260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.21395497365481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08754974417283	57.63691521634999
2	7.750994883456509	10.88456011496088
3	4.503695281409891	9.486667731119272
4	2.5264354747015347	7.095641066581511
5	1.3189312109152929	4.630368832827719
6	0.7254121660034111	3.0560434296662944
7	0.3479249573621376	1.7100431103305125
8	0.2376350198976691	1.3348235669806803
9	0.14326321773735076	0.9053169407632126
>10	0.35815804434337695	3.2596199904199263
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001596678907871627	0.0
2	0.0	0.0	0.0	0.005588376177550695	0.0
3	0.0	0.0	0.0	0.005588376177550695	0.0
4	0.0	0.0	0.0	0.005588376177550695	0.0
5	0.0	0.0	0.0	0.007185055085422321	0.0
6	0.0	0.0	0.0	0.007185055085422321	0.0
7	0.0	0.0	0.0	0.008781733993293949	0.0
8	0.0	0.0	0.0	0.008781733993293949	0.0
9	0.0	0.0	0.0	0.01117675235510139	0.0
10	0.0	0.0	0.0	0.01117675235510139	0.0
11	0.0	0.0	0.0	0.011975091809037202	0.0
12	0.0	0.0	0.0	0.011975091809037202	0.0
13	0.0	0.0	0.0	0.011975091809037202	0.0
14	0.0	0.0	0.0	0.011975091809037202	0.0
15	0.0	0.0	0.0	0.011975091809037202	0.0
16	0.0	0.0	0.0	0.011975091809037202	0.0
17	0.0	0.0	0.0	0.01357177071690883	0.0
18	0.0	0.0	0.0	0.017563467986587897	0.0
19	0.0	0.0	0.0	0.017563467986587897	0.0
20	0.0	0.0	0.0	0.021555165256266964	0.0
21	0.0	0.0	0.0	0.02474852307201022	0.0
22	0.0	0.0	0.0	0.030336899249560914	0.0
23	0.0	0.0	0.0	0.03193357815743254	0.0
24	0.0	0.0	0.0	0.035126935973175795	0.0
25	0.0	0.0	0.0	0.03911863324285486	0.0
26	0.0	0.0	0.0	0.046303688328277186	0.0
27	0.0	0.0	0.0	0.07264889030815903	0.0
28	0.0	0.0	0.0	0.10378412901165576	0.0
29	0.0	0.0	0.0	0.13172600989940922	0.0
30	0.0	0.0	0.0	0.1716429825961999	0.0
31	0.0	0.0	0.0	0.20437490020756827	0.0
32	0.0	0.0	0.0	0.2363084783650008	0.0
33	0.0	0.0	0.0	0.295385597956251	0.0
34	0.0	0.0	0.0	0.3792112406195114	0.0
35	0.0	0.0	0.0	0.4981638192559476	0.0
36	0.0	0.0	0.0	0.676193517483634	0.0
37	0.0	0.0	0.0	0.9867475650646655	0.0
38	0.0	0.0	0.0	1.4058757783809677	0.0
39	0.0	0.0	0.0	1.9311831390707328	0.0
40	0.0	0.0	0.0	2.5778380967587418	0.0
41	0.0	0.0	0.0	3.28756187130768	0.0
42	0.0	0.0	0.0	4.017244132205014	0.0
43	0.0	0.0	0.0	4.8674756506466546	0.0
44	0.0	0.0	0.0	5.609133003353025	0.0
45	0.0	0.0	0.0	6.342806961520038	0.0
46	0.0	0.0	0.0	7.047740699345361	0.0
47	0.0	0.0	0.0	7.795784767683219	0.0
48	0.0	0.0	0.0	8.58135079035606	0.0
49	0.0	0.0	0.0	9.215232316781096	0.0
50	0.0	0.0	0.0	9.8818457608175	0.0
51	0.0	0.0	0.0	10.607536324445155	0.0
52	0.0	0.0	0.0	11.31406674117835	0.0
53	0.0	0.0	0.0	11.97189845122146	0.0
54	0.0	0.0	0.0	12.61057001437011	0.0
55	0.0	0.0	0.0	13.25323327478844	0.0
56	0.0	0.0	0.0	13.835222736707648	0.0
57	0.0	0.0	0.0	14.47549097876417	0.0
58	0.0	0.0	0.0	15.061472137953057	0.0
59	0.0	0.0	0.0	15.557240938847197	0.0
60	0.0	0.0	0.0	16.0833466389909	0.0
61	0.0	0.0	0.0	16.610250678588535	0.0
62	0.0	0.0	0.0	17.26329235190803	0.0
63	0.0	0.0	0.0	17.80137314386077	0.0
64	0.0	0.0	0.0	18.377774229602426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATC	35	3.6779966E-9	70.0	23
ACGATCT	35	2.9544572E-7	60.0	24
CTGTGTA	60	1.8189894E-12	58.333336	34
ATCTCGG	105	0.0	56.666664	42
TAGATCT	125	0.0	56.0	39
TCGGTGG	75	0.0	56.0	45
AGCCGTA	25	2.3827922E-4	55.999996	53
GATCTCG	100	0.0	55.999996	41
CGATCTA	25	2.3827922E-4	55.999996	25
CGACGAT	95	0.0	55.26316	22
CAGCCGT	20	0.0069376566	52.5	12
CCGGACC	20	0.0069376566	52.5	53
AACGGAC	20	0.0069376566	52.5	35
ACGTGGG	20	0.0069376566	52.5	14
TTACGTG	20	0.0069376566	52.5	12
CGGTATA	20	0.0069376566	52.5	67
CGACTAT	20	0.0069376566	52.5	22
CGTTTTC	20	0.0069376566	52.5	40
GCCGTAA	20	0.0069376566	52.5	54
GGTAGCC	20	0.0069376566	52.5	50
>>END_MODULE