FastQCFastQC Report
Wed 25 May 2016
SRR1779796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125260
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2145717.12996966310075No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG27302.179466709244771No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC16121.2869231997445314No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT4800.38320293788919046Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3780.30177231358773754No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT3540.282612166693278No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3400.2714354143381766No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1600.12773431262973017No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1510.12054925754430784No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT1490.11895257863643621No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1390.11096918409707808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGC150.00222036769.9991914
ACGTTAG150.00222036769.9991970
TTACTCG150.00222036769.9991919
CCCACGT150.00222036769.9991959
ATCGTAT301.0169242E-769.9991939
ACGATCT150.00222036769.9991934
GAGCGCC150.00222036769.9991928
CGAGACA150.00222036769.9991923
CACGTAA150.00222036769.9991961
TATACCG150.00222036769.999196
CCACGTA150.00222036769.9991960
TAAGCGC150.00222036769.9991947
AGATATC308.337325E-658.3326635
GCTTATA252.3735341E-456.0441061
AAAGACG252.3829266E-455.9993666
CCGTCTT32100.054.6255447
CGTCTTC32250.054.58852448
GCCGTCT32150.054.5405946
TATGCCG32350.054.5279743
ATGCCGT32350.054.5279744
Produced by FastQC (version 0.11.5)