##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779796_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125260
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59740539677471	34.0	34.0	34.0	34.0	34.0
2	33.67215391984672	34.0	34.0	34.0	34.0	34.0
3	33.75942040555644	34.0	34.0	34.0	34.0	34.0
4	33.60490180424716	34.0	34.0	34.0	34.0	34.0
5	33.63866357975411	34.0	34.0	34.0	34.0	34.0
6	37.34087498004151	38.0	38.0	38.0	37.0	38.0
7	37.40245090212358	38.0	38.0	38.0	37.0	38.0
8	37.30178029698228	38.0	38.0	38.0	37.0	38.0
9	37.239589653520675	38.0	38.0	38.0	36.0	38.0
10-11	37.17495609133003	38.0	38.0	38.0	36.5	38.0
12-13	37.33186172760658	38.0	38.0	38.0	36.5	38.0
14-15	37.353436851349194	38.0	38.0	38.0	37.0	38.0
16-17	37.24260737665655	38.0	38.0	38.0	36.0	38.0
18-19	37.09309037202618	38.0	38.0	38.0	36.0	38.0
20-21	37.13963356219064	38.0	38.0	38.0	36.0	38.0
22-23	37.13704295066262	38.0	38.0	38.0	36.0	38.0
24-25	37.27633322688807	38.0	38.0	38.0	36.0	38.0
26-27	37.19948107935494	38.0	38.0	38.0	36.0	38.0
28-29	36.77297620948427	38.0	37.0	38.0	34.5	38.0
30-31	36.99227606578317	38.0	38.0	38.0	35.5	38.0
32-33	37.02044946511256	38.0	38.0	38.0	36.0	38.0
34-35	37.007444515407954	38.0	38.0	38.0	36.0	38.0
36-37	37.034943317898765	38.0	38.0	38.0	36.0	38.0
38-39	35.93570174038001	38.0	37.0	38.0	32.5	38.0
40-41	36.6942998562989	38.0	37.0	38.0	35.0	38.0
42-43	37.19101868114322	38.0	38.0	38.0	36.5	38.0
44-45	36.9899289477886	38.0	38.0	38.0	35.5	38.0
46-47	37.127542711160785	38.0	38.0	38.0	36.0	38.0
48-49	36.78933418489542	38.0	37.0	38.0	34.5	38.0
50-51	36.92129969663101	38.0	37.5	38.0	35.0	38.0
52-53	36.832991377933894	38.0	37.5	38.0	35.0	38.0
54-55	36.827311192719144	38.0	37.5	38.0	35.0	38.0
56-57	36.722189845122145	38.0	37.0	38.0	34.5	38.0
58-59	36.79652323167811	38.0	37.0	38.0	35.0	38.0
60-61	36.45612725530896	38.0	37.0	38.0	34.0	38.0
62-63	36.321167970621104	38.0	37.0	38.0	34.0	38.0
64-65	36.69144180105381	38.0	37.0	38.0	34.5	38.0
66-67	36.41553967747086	38.0	37.0	38.0	34.0	38.0
68-69	36.45108574165735	38.0	37.0	38.0	34.0	38.0
70-71	35.07266884879451	38.0	35.5	38.0	28.0	38.0
72-73	32.59600830273032	38.0	30.0	38.0	16.0	38.0
74-75	32.2798578955772	38.0	28.5	38.0	11.0	38.0
76	32.12166693277982	38.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	5.0
23	9.0
24	32.0
25	55.0
26	136.0
27	205.0
28	436.0
29	728.0
30	1189.0
31	1753.0
32	2875.0
33	4675.0
34	8216.0
35	17873.0
36	21603.0
37	65470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.01623055049682	14.270423975206874	11.091408671203553	41.62193680309275
2	16.389908989302253	15.692958646016287	45.026345201979886	22.89078716270158
3	15.444675075842248	16.988663579754114	43.80249081909628	23.76417052530736
4	11.367555484592048	31.56793868752994	31.6852945872585	25.379211240619508
5	11.185534089094682	28.963755388791313	47.05492575443078	12.79578476768322
6	23.49513012933099	48.2077279259141	16.12885198786524	12.16828995688967
7	22.740699345361648	25.505348874341372	17.826121666932778	33.9278301133642
8	19.96567140348076	46.434615998722656	15.2474852307201	18.35222736707648
9	19.43796902442919	15.860609931342806	15.714513811272553	48.98690723295545
10-11	20.111368353824048	30.563228484751715	29.369711001117675	19.955692160306562
12-13	19.63595720900527	18.206929586460163	31.45297780616318	30.704135398371385
14-15	17.889589653520677	17.939086699664696	20.08542232157113	44.08590132524349
16-17	29.326201500878174	29.740938847197828	18.901085741657354	22.031773910266644
18-19	29.732157113204533	22.457288839214435	19.49784448347437	28.312709564106658
20-21	18.740619511416252	19.997604981638194	19.716589493852787	41.54518601309277
22-23	18.55261057001437	31.0621906434616	19.286683697908348	31.09851508861568
24-25	29.165003432914467	30.78206581615546	19.31293809577033	20.73999265515975
26-27	29.38288360210762	31.63460003193358	20.265447868433657	18.717068497525148
28-29	18.76417052530736	22.177870030336898	30.033530257065305	29.024429187290433
30-31	27.58023311512055	19.89701421044228	33.69830752035766	18.824445154079513
32-33	20.03073606897653	20.130528500718505	30.639469902602585	29.19926552770238
34-35	18.0360849433179	40.23072010218745	22.78820054287083	18.94499441162382
36-37	29.028820054287085	32.55388791314067	20.109771674916175	18.307520357656077
38-39	18.701900047900367	22.428149449145778	30.859013252434934	28.010937250518918
40-41	17.659667890787162	18.984113044866678	32.85725690563628	30.498962158709887
42-43	28.55791377101138	18.748278573681038	31.70817383112658	20.985633824181
44-45	18.055245090212356	29.534568098355418	33.487945074245566	18.922241737186653
46-47	27.782612166693276	19.226808238863164	24.175315344084307	28.815264250359252
48-49	26.98307520357656	18.727446910426313	22.01939964873064	32.27007823726649
50-51	18.477974465231036	18.43246221285362	33.264665724483194	29.824897597432148
52-53	16.157138978069547	18.830162761352565	43.644480649128695	21.3682176114492
54-55	15.278138316353632	19.468683730236386	34.459678031286316	30.79349992212367
56-57	26.32243222085537	18.973367628968095	24.50873100825971	30.195469141916824
58-59	16.302620334949246	19.279946970362502	42.28075359587263	22.136679098815616
60-61	26.323082513707025	29.30408635127526	24.025333540985308	20.3474975940324
62-63	15.825766831971762	42.95416976114612	20.283813676401298	20.93624973048082
64-65	15.50893302724823	43.912965365804965	19.61153807765246	20.96656352929434
66-67	15.344589360019809	43.80026675824834	19.21258396096065	21.642559920771205
68-69	15.564817476286288	44.130257736897576	18.793315448245025	21.51160933857111
70-71	16.257196010954704	42.21633145165798	19.195883203053267	22.330589334334057
72-73	17.026688174790774	36.7453523286271	20.407030601162717	25.82092889541941
74-75	17.306440859313554	34.96221240103961	20.927902139500716	26.803444600146122
76	17.18821115165646	35.316240408186076	20.988206360740037	26.507342079417423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	4.5
15	5.0
16	12.5
17	22.0
18	25.5
19	41.5
20	66.5
21	79.0
22	105.0
23	170.5
24	248.5
25	287.0
26	339.0
27	438.0
28	574.5
29	664.0
30	769.0
31	1003.5
32	1294.5
33	1456.0
34	1653.5
35	2069.5
36	2573.0
37	2858.0
38	3211.5
39	7059.5
40	12377.5
41	13348.5
42	12496.0
43	10965.5
44	8411.0
45	6756.5
46	6126.0
47	5923.5
48	5410.5
49	4906.0
50	4712.0
51	4561.5
52	4016.5
53	3440.5
54	3259.0
55	3071.0
56	2732.5
57	2429.5
58	2277.0
59	1984.5
60	1649.0
61	1452.5
62	1299.0
63	1223.0
64	1127.0
65	1061.0
66	986.5
67	958.0
68	835.0
69	697.5
70	644.0
71	605.0
72	553.5
73	508.5
74	479.0
75	443.0
76	429.0
77	383.0
78	294.5
79	238.0
80	246.5
81	219.0
82	141.0
83	99.0
84	106.5
85	87.0
86	60.5
87	61.0
88	41.5
89	18.5
90	9.0
91	3.5
92	4.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05109372505189207
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001596678907871627
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.9916972696790677E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015168449624780455
52-53	0.03672361488104742
54-55	0.04909787641705254
56-57	0.0590771195912502
58-59	0.03752195433498323
60-61	0.04031614242375858
62-63	0.0295385597956251
64-65	0.0179626377135558
66-67	0.04231199105859811
68-69	0.012773431262973017
70-71	0.013571770716908831
72-73	0.028740220341689285
74-75	0.01636595880568418
76	0.01836180744052371
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	125260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.41306083346639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.77633368596041	10.506147213795305
2	17.225328698806106	9.099473095960402
3	14.408342149010126	11.417052530736068
4	10.593924739307843	11.192719144180105
5	6.6374489950128455	8.765767204215233
6	3.9081154601783283	6.193517483634041
7	2.3938340637751248	4.42599393262015
8	1.3087501889073598	2.765447868433658
9	0.9248904337312982	2.1986268561392306
>10	2.75049115913556	10.392783011336421
>50	0.03324769533021007	0.5460641864920964
>100	0.03022517757291824	1.9000479003672361
>500	0.0	0.0
>1k	0.006045035514583648	3.466389908989302
>5k	0.0	0.0
>10k+	0.003022517757291824	17.12996966310075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	21457	17.12996966310075	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2730	2.179466709244771	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1612	1.2869231997445314	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	480	0.38320293788919046	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	378	0.30177231358773754	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	354	0.282612166693278	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	340	0.2714354143381766	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	160	0.12773431262973017	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	151	0.12054925754430784	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	149	0.11895257863643621	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	139	0.11096918409707808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001596678907871627	0.0
2	0.0	0.0	0.0	0.005588376177550695	0.0
3	0.0	0.0	0.0	0.005588376177550695	0.0
4	0.0	0.0	0.0	0.005588376177550695	0.0
5	0.0	0.0	0.0	0.007983394539358136	0.0
6	0.0	0.0	0.0	0.007983394539358136	0.0
7	0.0	0.0	0.0	0.010378412901165576	0.0
8	0.0	0.0	0.0	0.010378412901165576	0.0
9	0.0	0.0	0.0	0.01357177071690883	0.0
10	0.0	0.0	0.0	0.01357177071690883	0.0
11	0.0	0.0	0.0	0.014370110170844642	0.0
12	0.0	0.0	0.0	0.014370110170844642	0.0
13	0.0	0.0	0.0	0.014370110170844642	0.0
14	0.0	0.0	0.0	0.014370110170844642	0.0
15	0.0	0.0	0.0	0.014370110170844642	0.0
16	0.0	0.0	0.0	0.014370110170844642	0.0
17	0.0	0.0	0.0	0.01596678907871627	0.0
18	0.0	0.0	0.0	0.020756825802331152	0.0
19	0.0	0.0	0.0	0.020756825802331152	0.0
20	0.0	0.0	0.0	0.025546862525946033	0.0
21	0.0	0.0	0.0	0.02714354143381766	0.0
22	0.0	0.0	0.0	0.03193357815743254	0.0
23	0.0	0.0	0.0	0.03273191761136835	0.0
24	0.0	0.0	0.0	0.03592527542711161	0.0
25	0.0	0.0	0.0	0.04470700942040556	0.0
26	0.0	0.0	0.0	0.053488743413699504	0.0
27	0.0	0.0	0.0	0.08063228484751717	0.0
28	0.0	0.0	0.0	0.11336420245888552	0.0
29	0.0	0.0	0.0	0.1421044228005748	0.0
30	0.0	0.0	0.0	0.18441641385917293	0.0
31	0.0	0.0	0.0	0.2187450103784129	0.0
32	0.0	0.0	0.0	0.2538719463515887	0.0
33	0.0	0.0	0.0	0.3129490659428389	0.0
34	0.0	0.0	0.0	0.3967747086060993	0.0
35	0.0	0.0	0.0	0.5292990579594443	0.0
36	0.0	0.0	0.0	0.7177071690882963	0.0
37	0.0	0.0	0.0	1.0490180424716589	0.0
38	0.0	0.0	0.0	1.4737346319655118	0.0
39	0.0	0.0	0.0	2.0046303688328275	0.0
40	0.0	0.0	0.0	2.6832189046782693	0.0
41	0.0	0.0	0.0	3.4184895417531536	0.0
42	0.0	0.0	0.0	4.1777103624461125	0.0
43	0.0	0.0	0.0	5.058278780137314	0.0
44	0.0	0.0	0.0	5.8374580871786685	0.0
45	0.0	0.0	0.0	6.600670605141306	0.0
46	0.0	0.0	0.0	7.352706370748843	0.0
47	0.0	0.0	0.0	8.1119271914418	0.0
48	0.0	0.0	0.0	8.946990260258662	0.0
49	0.0	0.0	0.0	9.623982117196231	0.0
50	0.0	0.0	0.0	10.313747405396775	0.0
51	0.0	0.0	0.0	11.055404758103146	0.0
52	0.0	0.0	0.0	11.772313587737505	0.0
53	0.0	0.0	0.0	12.42375858214913	0.0
54	0.0	0.0	0.0	13.068816860929267	0.0
55	0.0	0.0	0.0	13.723455213156635	0.0
56	0.0	0.0	0.0	14.299057959444356	0.0
57	0.0	0.0	0.0	14.943317898770557	0.0
58	0.0	0.0	0.0	15.527702379051572	0.0
59	0.0	0.0	0.0	16.033849592846877	0.0
60	0.0	0.0	0.0	16.563148650806323	0.0
61	0.0	0.0	0.0	17.094044387673637	0.0
62	0.0	0.0	0.0	17.76464952897972	0.0
63	0.0	0.0	0.0	18.315503752195433	0.0
64	0.0	0.0	0.0	18.90148491138432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGC	15	0.002220367	69.99919	14
ACGTTAG	15	0.002220367	69.99919	70
TTACTCG	15	0.002220367	69.99919	19
CCCACGT	15	0.002220367	69.99919	59
ATCGTAT	30	1.0169242E-7	69.99919	39
ACGATCT	15	0.002220367	69.99919	34
GAGCGCC	15	0.002220367	69.99919	28
CGAGACA	15	0.002220367	69.99919	23
CACGTAA	15	0.002220367	69.99919	61
TATACCG	15	0.002220367	69.99919	6
CCACGTA	15	0.002220367	69.99919	60
TAAGCGC	15	0.002220367	69.99919	47
AGATATC	30	8.337325E-6	58.33266	35
GCTTATA	25	2.3735341E-4	56.044106	1
AAAGACG	25	2.3829266E-4	55.99936	66
CCGTCTT	3210	0.0	54.62554	47
CGTCTTC	3225	0.0	54.588524	48
GCCGTCT	3215	0.0	54.54059	46
TATGCCG	3235	0.0	54.52797	43
ATGCCGT	3235	0.0	54.52797	44
>>END_MODULE