FastQCFastQC Report
Wed 25 May 2016
SRR1779795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248794
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA252.3885019E-455.99469449
CATCGTT200.006942747652.5055832
GCTTAAG200.006948270452.4950221
TGCGTGA305.867399E-446.67162332
GACGCTT305.8732176E-446.66224314
CGCTTAT305.8732176E-446.66224350
GACGACA551.3080353E-744.54123323
CAAGTCT404.5815024E-543.7546534
CTTCCGA757.530616E-1041.9960218
GTAGATC855.638867E-1141.1725738
ACGTGTA350.001253217540.00425334
TAGATCT1250.039.19628539
TGGTCGC556.6824177E-638.178249
CGGTGGT654.853264E-737.68873646
CTCGGTG753.526111E-837.32979644
TCGGTGG753.526111E-837.32979645
CGCATGT400.00241216235.01075763
GTCGCTT400.002416926234.9966848
GTTCTAC400.002416926234.996681
GGTCCAA400.002416926234.9966811