##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779795_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248794
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.456827737003305	34.0	27.0	34.0	12.0	34.0
2	30.127824625995803	34.0	31.0	34.0	12.0	34.0
3	29.89369920496475	34.0	31.0	34.0	11.0	34.0
4	29.985598527295675	34.0	31.0	34.0	12.0	34.0
5	30.469086875085413	34.0	32.0	34.0	21.0	34.0
6	33.288511780830724	37.0	34.0	38.0	21.0	38.0
7	33.51949001985579	38.0	34.0	38.0	21.0	38.0
8	32.89244113604026	37.0	34.0	38.0	11.0	38.0
9	33.81596421135558	38.0	34.0	38.0	21.0	38.0
10-11	32.36239017018095	37.0	30.5	38.0	16.5	38.0
12-13	33.24154119472335	37.0	34.0	38.0	16.0	38.0
14-15	31.987809191539988	37.0	29.0	38.0	11.0	38.0
16-17	32.65284733554668	37.0	31.0	38.0	16.0	38.0
18-19	33.57536958286775	38.0	34.0	38.0	21.0	38.0
20-21	32.19184747220592	37.0	30.0	38.0	16.0	38.0
22-23	33.13283077566179	37.5	32.5	38.0	16.0	38.0
24-25	33.44419479569443	38.0	34.0	38.0	21.0	38.0
26-27	32.33118965891461	37.0	31.0	38.0	11.0	38.0
28-29	33.6178263945272	37.5	34.0	38.0	21.0	38.0
30-31	34.04690426618005	38.0	36.0	38.0	21.0	38.0
32-33	34.143888116272905	38.0	36.0	38.0	21.0	38.0
34-35	34.04464536926132	38.0	35.5	38.0	21.0	38.0
36-37	33.53365434857754	38.0	34.0	38.0	16.0	38.0
38-39	33.49104279042099	38.0	34.0	38.0	16.0	38.0
40-41	33.48676415026085	38.0	34.0	38.0	21.0	38.0
42-43	33.87051536612619	38.0	35.0	38.0	21.5	38.0
44-45	33.92493589073692	38.0	34.5	38.0	21.0	38.0
46-47	33.821117068739596	38.0	34.5	38.0	21.0	38.0
48-49	33.55848211773596	38.0	34.0	38.0	16.0	38.0
50-51	32.90940095018368	37.5	32.5	38.0	16.0	38.0
52-53	33.488221178967336	37.5	34.0	38.0	21.0	38.0
54-55	33.652017331607674	38.0	34.5	38.0	21.0	38.0
56-57	33.698740725258645	38.0	34.0	38.0	21.0	38.0
58-59	33.963485855768226	38.0	35.0	38.0	21.0	38.0
60-61	33.82297804609436	38.0	34.5	38.0	21.0	38.0
62-63	33.827941992170224	38.0	35.0	38.0	21.0	38.0
64-65	33.83771513782487	38.0	34.5	38.0	21.0	38.0
66-67	33.68095090717622	38.0	34.0	38.0	21.0	38.0
68-69	33.05690651703819	37.5	33.5	38.0	15.0	38.0
70-71	33.11851772952724	37.0	33.5	38.0	19.0	38.0
72-73	33.37399615746361	37.0	34.0	38.0	20.5	38.0
74-75	33.46209313729431	37.5	34.0	38.0	20.5	38.0
76	32.43259081810655	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	26.0
13	359.0
14	1426.0
15	2959.0
16	4019.0
17	4525.0
18	4531.0
19	3975.0
20	3328.0
21	2513.0
22	2077.0
23	2045.0
24	1988.0
25	2172.0
26	2423.0
27	3028.0
28	3673.0
29	4406.0
30	5351.0
31	6624.0
32	8751.0
33	11546.0
34	15919.0
35	24150.0
36	42398.0
37	84579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.48213560779932	18.653809824310365	9.784919872503426	32.07913469538689
2	20.377099126184714	19.95064189650876	30.63739479247892	29.034864184827608
3	20.655240882014837	20.397598012813813	30.051769737212314	28.895391367959032
4	15.760428306148864	19.762936405218774	31.971430179184384	32.50520510944798
5	13.76480140196307	32.46460927514329	33.29099576356343	20.47959355933021
6	28.71096105148921	38.1751678122111	16.487399011214276	16.626472125085414
7	27.49307618408306	30.87238070737482	19.274938801104593	22.359604307437525
8	24.244239094172173	35.35646672481501	19.18935322703174	21.209940953981086
9	24.563356955939753	17.21649857500613	19.52583278732017	38.69431168173395
10-11	24.11772981229149	25.35471682945456	24.655733751356564	25.871819606897382
12-13	24.305297365083895	22.517670276755624	25.662245999192102	27.51478635896838
14-15	24.60639969774069	24.320820923418264	22.82399424421105	28.248785134629994
16-17	25.409630152155522	25.894976315509172	23.376401524971712	25.318992007363594
18-19	22.883631379453107	25.808221490672743	25.259961332384727	26.04818579748942
20-21	24.016254411280013	25.100082799424424	25.339437446240666	25.5442253430549
22-23	25.592766619371282	24.875497907882522	24.318001856980356	25.213733615765843
24-25	22.907857292152357	27.43319613486181	24.06848401048282	25.590462562503014
26-27	23.231515292803067	27.40787038246578	25.04245488728604	24.31815943744511
28-29	23.124943472449726	26.878573797344963	26.063170165512666	23.933312564692642
30-31	23.50458612345957	26.09106328930762	25.74700354510157	24.657347042131242
32-33	22.1356265336514	27.3120077493986	25.833271367620135	24.719094349329865
34-35	22.48105794159616	27.693590851538474	25.488674960307094	24.336676246558273
36-37	24.3320930999202	25.71263168685815	25.34598894041452	24.60928627280713
38-39	24.005306106041726	25.275153756481888	26.406520078787636	24.313020058688746
40-41	22.65324168977853	25.886691587282446	26.43152859841634	25.028538124522694
42-43	21.36612658818044	25.320047750923464	27.060464405866774	26.253361255029322
44-45	20.76670766707667	24.19406056805666	28.596982048251856	26.442249716614814
46-47	21.110999457362784	25.048133930904193	28.080471089494946	25.760395522238078
48-49	20.823494182926805	25.375662949393774	28.233253682270636	25.567589185408785
50-51	22.019104580798547	25.679040630897326	26.72551183032978	25.576342957974347
52-53	19.037100165220153	26.54939117780663	27.850047636467423	26.56346102050579
54-55	19.339842588051805	25.740632059620378	27.498050439354593	27.42147491297322
56-57	19.946333276383626	26.18815513100107	26.97647307116368	26.889038521451614
58-59	20.067733247578083	26.069260762953732	26.640069140169636	27.222936849298552
60-61	19.43976521197258	25.445353488652582	27.055299817074395	28.059581482300437
62-63	20.219163544564324	25.470928269363736	26.5936791497162	27.716229036355745
64-65	20.99698067390072	25.033268872798324	25.5380310455874	28.43171940771355
66-67	20.286787628816246	26.774081377095182	24.451519664891833	28.487611329196742
68-69	20.96405639237517	27.400745601254055	23.983239044585346	27.65195896178542
70-71	20.877839967040806	27.93150918938473	24.11628164032276	27.07436920325171
72-73	21.336264937716805	28.3134297761503	23.65073176221848	26.699573523914417
74-75	22.030818827341005	28.650817098881927	23.29179002035964	26.026574053417423
76	21.890877226831307	29.901038652003344	22.72654189980063	25.481542221364716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	1.0
11	1.5
12	1.0
13	3.5
14	6.0
15	12.5
16	19.5
17	20.0
18	36.0
19	74.5
20	121.5
21	146.0
22	173.5
23	249.0
24	400.0
25	503.0
26	642.0
27	915.0
28	1193.0
29	1337.0
30	1627.5
31	2243.0
32	2919.5
33	3271.0
34	3789.5
35	4968.5
36	6158.0
37	6687.0
38	7317.5
39	9093.0
40	11050.0
41	12333.0
42	12804.0
43	13195.5
44	14104.0
45	14920.0
46	15219.0
47	15391.0
48	15233.0
49	14397.0
50	13891.0
51	13302.5
52	11770.0
53	10063.0
54	9300.0
55	8726.5
56	7480.5
57	6202.0
58	5596.0
59	5128.5
60	4328.5
61	3721.5
62	3447.0
63	3270.5
64	2855.5
65	2482.5
66	2257.5
67	2167.0
68	2036.5
69	1792.5
70	1576.5
71	1474.0
72	1423.5
73	1299.0
74	1149.5
75	1074.0
76	1014.5
77	876.0
78	741.0
79	685.0
80	657.5
81	541.5
82	389.5
83	326.0
84	296.0
85	238.0
86	165.5
87	121.0
88	104.5
89	87.5
90	70.5
91	44.5
92	35.0
93	29.5
94	22.0
95	17.5
96	15.0
97	13.0
98	9.0
99	12.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001205816860535222
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0016077558140469624
7	0.0060290843026761095
8	0.002009694767558703
9	0.008440718023746553
10-11	0.0016077558140469624
12-13	2.009694767558703E-4
14-15	4.019389535117406E-4
16-17	0.0010048473837793515
18-19	0.0028135726745821842
20-21	0.0
22-23	0.001205816860535222
24-25	0.002411633721070444
26-27	2.009694767558703E-4
28-29	0.008641687500502425
30-31	0.0
32-33	2.009694767558703E-4
34-35	0.003617450581605666
36-37	0.020297917152342904
38-39	0.009646534884281776
40-41	0.0016077558140469624
42-43	0.001205816860535222
44-45	0.00643102325618785
46-47	0.003617450581605666
48-49	2.009694767558703E-4
50-51	0.0022106642443145736
52-53	0.01406786337291092
54-55	0.008038779070234812
56-57	0.014670771803178533
58-59	0.009646534884281776
60-61	0.023714398257192695
62-63	0.012058168605352219
64-65	0.02532215407123966
66-67	0.015474649710202015
68-69	6.02908430267611E-4
70-71	6.02908430267611E-4
72-73	0.004421328488629147
74-75	0.00703393168645546
76	0.004019389535117406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248794.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.85723243551085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8057194446509	46.741835206197564
2	9.877241858675259	12.02203207230315
3	5.555268119129601	10.142347295023601
4	3.079725672614227	7.49694324383576
5	1.6928066618128477	5.150976424316284
6	0.8706454714287005	3.179104429419681
7	0.49800722558645666	2.121513903745522
8	0.326286185666238	1.5885499393269196
9	0.2354909326781779	1.2898193783805998
>10	1.054749196587107	10.1083648969214
>50	0.0033826926420653445	0.11445888968109103
>100	6.765385284130689E-4	0.04405432084847748
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.0193895351174066E-4	0.0
2	0.0	0.0	0.0	4.0193895351174066E-4	0.0
3	0.0	0.0	0.0	4.0193895351174066E-4	0.0
4	0.0	0.0	0.0	8.038779070234813E-4	0.0
5	0.0	0.0	0.0	0.0024116337210704436	0.0
6	0.0	0.0	0.0	0.0024116337210704436	0.0
7	0.0	0.0	0.0	0.0032155116280939253	4.0193895351174066E-4
8	0.0	0.0	0.0	0.004019389535117406	4.0193895351174066E-4
9	0.0	0.0	0.0	0.005225206395652628	4.0193895351174066E-4
10	0.0	0.0	0.0	0.007234901163211332	4.0193895351174066E-4
11	0.0	0.0	0.0	0.007636840116723072	4.0193895351174066E-4
12	0.0	0.0	0.0	0.007636840116723072	4.0193895351174066E-4
13	0.0	0.0	0.0	0.008038779070234812	4.0193895351174066E-4
14	0.0	0.0	0.0	0.008038779070234812	4.0193895351174066E-4
15	0.0	0.0	0.0	0.008038779070234812	4.0193895351174066E-4
16	0.0	0.0	0.0	0.008038779070234812	4.0193895351174066E-4
17	0.0	0.0	0.0	0.008440718023746553	4.0193895351174066E-4
18	0.0	0.0	0.0	0.009646534884281775	4.0193895351174066E-4
19	0.0	0.0	0.0	0.011656229651840478	4.0193895351174066E-4
20	0.0	0.0	0.0	0.014469802326422664	4.0193895351174066E-4
21	0.0	0.0	0.0	0.014871741279934403	4.0193895351174066E-4
22	0.0	0.0	0.0	0.02492021511772792	4.0193895351174066E-4
23	0.0	0.0	0.0	0.026929909885286624	4.0193895351174066E-4
24	0.0	0.0	0.0	0.036174505816056655	4.0193895351174066E-4
25	0.0	0.0	0.0	0.03979195639766232	4.0193895351174066E-4
26	0.0	0.0	0.0	0.04300746802575625	4.0193895351174066E-4
27	0.0	0.0	0.0	0.07677034012074246	4.0193895351174066E-4
28	0.0	0.0	0.0	0.11495454070435782	4.0193895351174066E-4
29	0.0	0.0	0.0	0.16157945931171974	4.0193895351174066E-4
30	0.0	0.0	0.0	0.20981213373312863	4.0193895351174066E-4
31	0.0	0.0	0.0	0.266485526178284	4.0193895351174066E-4
32	0.0	0.0	0.0	0.3215511628093925	4.0193895351174066E-4
33	0.0	0.0	0.0	0.3886749680458532	4.0193895351174066E-4
34	0.0	0.0	0.0	0.48112092735355355	4.0193895351174066E-4
35	0.0	0.0	0.0	0.6173782325940336	4.0193895351174066E-4
36	0.0	0.0	0.0	0.8750211017950593	4.0193895351174066E-4
37	0.0	0.0	0.0	1.3163500727509505	4.0193895351174066E-4
38	0.0	0.0	0.002009694767558703	1.913631357669397	4.0193895351174066E-4
39	0.0	0.0	0.002009694767558703	2.595319822825309	4.0193895351174066E-4
40	0.0	0.0	0.002009694767558703	3.4176869217103305	4.0193895351174066E-4
41	0.0	0.0	0.002009694767558703	4.382340410138508	4.0193895351174066E-4
42	0.0	0.0	0.002009694767558703	5.356640433450967	4.0193895351174066E-4
43	0.0	0.0	0.002009694767558703	6.357468427695202	4.0193895351174066E-4
44	0.0	0.0	0.002009694767558703	7.31086762542505	4.0193895351174066E-4
45	0.0	0.0	0.002009694767558703	8.376809730138186	4.0193895351174066E-4
46	0.0	0.0	0.002009694767558703	9.31734688135566	4.0193895351174066E-4
47	0.0	0.0	0.002009694767558703	10.3354582506009	4.0193895351174066E-4
48	0.0	0.0	0.002009694767558703	11.172295151812342	4.0193895351174066E-4
49	0.0	0.0	0.002009694767558703	12.00752429720974	4.0193895351174066E-4
50	0.0	0.0	0.002009694767558703	12.857223244933559	4.0193895351174066E-4
51	0.0	0.0	0.002009694767558703	13.77404599789384	4.0193895351174066E-4
52	0.0	0.0	0.002009694767558703	14.697299774110308	4.0193895351174066E-4
53	0.0	0.0	0.002009694767558703	15.631405902071593	4.0193895351174066E-4
54	0.0	0.0	0.002009694767558703	16.53134721898438	4.0193895351174066E-4
55	0.0	0.0	0.002009694767558703	17.364968608567732	4.0193895351174066E-4
56	0.0	0.0	0.002009694767558703	18.158396102799905	4.0193895351174066E-4
57	0.0	0.0	0.002009694767558703	18.932932466217032	4.0193895351174066E-4
58	0.0	0.0	0.002009694767558703	19.680136980795357	4.0193895351174066E-4
59	0.0	0.0	0.002009694767558703	20.342532376182707	4.0193895351174066E-4
60	0.0	0.0	0.002009694767558703	21.00412389366303	4.0193895351174066E-4
61	0.0	0.0	0.002009694767558703	21.686214297772455	4.0193895351174066E-4
62	0.0	0.0	0.002009694767558703	22.297965385017324	4.0193895351174066E-4
63	0.0	0.0	0.002009694767558703	22.968399559474907	4.0193895351174066E-4
64	0.0	0.0	0.002009694767558703	23.606276678698038	4.0193895351174066E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	25	2.3885019E-4	55.994694	49
CATCGTT	20	0.0069427476	52.50558	32
GCTTAAG	20	0.0069482704	52.49502	21
TGCGTGA	30	5.867399E-4	46.671623	32
GACGCTT	30	5.8732176E-4	46.662243	14
CGCTTAT	30	5.8732176E-4	46.662243	50
GACGACA	55	1.3080353E-7	44.541233	23
CAAGTCT	40	4.5815024E-5	43.75465	34
CTTCCGA	75	7.530616E-10	41.99602	18
GTAGATC	85	5.638867E-11	41.17257	38
ACGTGTA	35	0.0012532175	40.004253	34
TAGATCT	125	0.0	39.196285	39
TGGTCGC	55	6.6824177E-6	38.1782	49
CGGTGGT	65	4.853264E-7	37.688736	46
CTCGGTG	75	3.526111E-8	37.329796	44
TCGGTGG	75	3.526111E-8	37.329796	45
CGCATGT	40	0.002412162	35.010757	63
GTCGCTT	40	0.0024169262	34.99668	48
GTTCTAC	40	0.0024169262	34.99668	1
GGTCCAA	40	0.0024169262	34.99668	11
>>END_MODULE