FastQCFastQC Report
Wed 25 May 2016
SRR1779795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248794
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT193627.782342017894322No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG26841.0788041512255118No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC15430.6201918052686158No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4450.1788628343127246RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT4060.1631872151257667No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT3790.1523348633809497No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3340.13424761047292139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA150.002224901769.9837130
ATCGTAT352.97121E-759.98604639
AACGTAT200.006952058552.4877938
TGCATCG200.006952058552.4877916
AGTCGAG200.006952058552.4877929
CGTCTTC28400.051.76933348
CCGTCTT28300.051.70487647
ATCTCGT27050.051.6146137
TCGTATG28950.051.61148540
TCTCGTA27000.051.5805938
TATGCCG28550.051.47664343
GCCGTCT28450.051.4322746
CGTATGC29000.051.28117441
CTCGTAT28850.051.06264539
TGCCGTC28750.050.9968345
GATCTCG27200.050.9440336
ATGCCGT28800.050.90829544
CGGATCT27250.050.72214534
GTATGCC29100.050.6239642
CTTGAAA28450.050.3451257
Produced by FastQC (version 0.11.5)