##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779795_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248794
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.5727710475333	34.0	34.0	34.0	34.0	34.0
2	33.65548204538695	34.0	34.0	34.0	34.0	34.0
3	33.75590649292185	34.0	34.0	34.0	34.0	34.0
4	33.59695973375564	34.0	34.0	34.0	34.0	34.0
5	33.61679542111144	34.0	34.0	34.0	34.0	34.0
6	37.309356334959844	38.0	38.0	38.0	37.0	38.0
7	37.37048723039945	38.0	38.0	38.0	37.0	38.0
8	37.27134496812624	38.0	38.0	38.0	36.0	38.0
9	37.18692974910971	38.0	38.0	38.0	36.0	38.0
10-11	37.14869932554643	38.0	38.0	38.0	36.0	38.0
12-13	37.29602000048233	38.0	38.0	38.0	36.5	38.0
14-15	37.313819867038596	38.0	38.0	38.0	36.5	38.0
16-17	37.24341623994148	38.0	38.0	38.0	36.0	38.0
18-19	37.135240399688094	38.0	38.0	38.0	36.0	38.0
20-21	37.139935046665116	38.0	38.0	38.0	36.0	38.0
22-23	37.12058168605352	38.0	38.0	38.0	36.0	38.0
24-25	37.306779906267835	38.0	38.0	38.0	36.5	38.0
26-27	37.217239965594025	38.0	38.0	38.0	36.0	38.0
28-29	36.77332451747229	38.0	37.0	38.0	34.5	38.0
30-31	37.1078522793958	38.0	38.0	38.0	36.0	38.0
32-33	37.08287378312981	38.0	38.0	38.0	36.0	38.0
34-35	37.16863951703016	38.0	38.0	38.0	36.0	38.0
36-37	37.11817005233245	38.0	38.0	38.0	36.0	38.0
38-39	36.44470324847062	38.0	37.0	38.0	34.0	38.0
40-41	36.876070162463726	38.0	37.5	38.0	35.0	38.0
42-43	37.214151064736285	38.0	38.0	38.0	36.5	38.0
44-45	36.991975288793135	38.0	38.0	38.0	35.5	38.0
46-47	37.127796490269056	38.0	38.0	38.0	36.0	38.0
48-49	36.809384470686595	38.0	37.0	38.0	35.0	38.0
50-51	36.97130959749833	38.0	37.5	38.0	35.5	38.0
52-53	36.89222006961583	38.0	38.0	38.0	35.5	38.0
54-55	36.89495526419447	38.0	37.5	38.0	35.5	38.0
56-57	36.77639533107711	38.0	37.0	38.0	35.0	38.0
58-59	36.86874884442551	38.0	37.5	38.0	35.5	38.0
60-61	36.5599913985064	38.0	37.0	38.0	34.0	38.0
62-63	36.40205149641872	38.0	37.0	38.0	34.0	38.0
64-65	36.737517785798694	38.0	37.0	38.0	34.5	38.0
66-67	36.542619596935616	38.0	37.0	38.0	34.5	38.0
68-69	36.63945071022613	38.0	37.0	38.0	34.0	38.0
70-71	36.056641237328876	38.0	37.0	38.0	32.5	38.0
72-73	34.93093482961808	38.0	37.0	38.0	23.5	38.0
74-75	34.773328536861825	38.0	37.0	38.0	23.5	38.0
76	34.47843999453363	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	6.0
23	15.0
24	53.0
25	108.0
26	208.0
27	421.0
28	643.0
29	1153.0
30	1878.0
31	3045.0
32	4858.0
33	7789.0
34	13212.0
35	25989.0
36	37578.0
37	151837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.90533258264296	16.705139547977158	10.832864151853936	33.556663717525936
2	19.582465815092004	17.82880616092028	37.887971574877206	24.700756449110507
3	18.373031503975177	19.38069246042911	35.24240938286293	27.003866652732782
4	13.14581541355499	23.16374189088161	34.104922144424705	29.585520551138693
5	13.048948125758661	32.046592763491084	40.242529964548986	14.661929146201274
6	27.896171129528845	41.25541612739857	17.31231460565769	13.53609813741489
7	26.80088748120935	27.759511885334852	19.610199602884315	25.829401030571475
8	24.01062726593085	39.739704333705795	16.982322724824552	19.2673456755388
9	22.751352524578568	16.45980208525929	18.2882223847842	42.50062300537794
10-11	23.439271043513912	27.35998456554419	27.28864040129585	21.912103989646052
12-13	23.288342966470253	20.820437791908162	28.234201789432223	27.657017452189365
14-15	21.467358537585312	21.10621638785501	21.438820871885977	35.987604202673694
16-17	26.328810180309816	28.34111755106634	22.04313608849088	23.28693618013296
18-19	26.45461707275899	24.563494296486247	23.16193316559081	25.81995546516395
20-21	21.444247047758385	23.504787092936326	22.8140550013264	32.23691085797889
22-23	21.557794802125454	28.31197697693674	22.92438724406537	27.20584097687243
24-25	26.31380091361642	28.219814583697595	22.981492534913773	22.48489196777221
26-27	26.128845550937722	29.01778177930336	23.67922859876042	21.174144070998498
28-29	21.24850277739817	28.69140734905183	24.024695129303762	26.035394744246243
30-31	20.827873662548132	24.17059896942852	33.19955465163951	21.801972716383837
32-33	25.74901323986913	23.350643504264575	25.179264773266237	25.721078482600063
34-35	25.97430002331246	23.051801892328594	23.743739800799055	27.23015828355989
36-37	27.364204924556056	27.584065532126978	23.171981639428605	21.879747903888358
38-39	21.014976245407848	24.574748587184576	28.635738803990453	25.774536363417123
40-41	20.6094198413145	22.37071633560295	29.902650385459456	27.117213437623093
42-43	25.55673681185401	22.1707962627641	29.58236449103373	22.690102434348162
44-45	20.406440669791074	26.627852761722547	30.74049213405468	22.2252144344317
46-47	24.72889217585633	21.856033505631164	26.754865471032257	26.660208847480245
48-49	23.84020514964187	22.17014879780059	25.975505840172996	28.014140212384543
50-51	19.770463795789155	22.099391990352242	31.16084618863374	26.969298025224862
52-53	18.2737769772603	22.44580425203435	35.68355794281303	23.596860827892314
54-55	17.676354387622773	22.694399485287462	31.754747519427383	27.874498607662378
56-57	22.58634660897156	22.63782179531777	26.580901499415877	28.19493009629479
58-59	18.195981778487198	22.354523414161477	34.8512566491233	24.598238158228025
60-61	22.83339833305859	26.872695692729547	26.41434711461529	23.87955885959657
62-63	18.235107285439174	33.237981899979495	23.91036027162092	24.61655054296041
64-65	17.953954324168954	34.037780448879005	23.032084035167415	24.976181191784622
66-67	17.951857123328427	33.93441568362564	22.40247991701445	25.71124727603149
68-69	18.370308436272715	34.13802251870194	22.098033934823594	25.39363511020175
70-71	18.587206673031506	33.55107783528466	21.948042811919	25.913672679764836
72-73	19.005091528926034	31.28466129171667	22.342826934248322	27.367420245108974
74-75	19.54568659530251	30.07183313167532	22.805913872597692	27.576566400424486
76	19.997025269132745	30.20879394762866	22.58182519838239	27.21235558485621
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	3.5
14	6.5
15	11.0
16	22.0
17	28.0
18	36.0
19	54.5
20	98.0
21	131.0
22	192.0
23	311.5
24	458.5
25	547.0
26	680.5
27	947.0
28	1240.0
29	1400.0
30	1639.0
31	2166.0
32	2873.0
33	3292.0
34	3695.0
35	4685.0
36	5823.0
37	6374.0
38	7029.0
39	8744.5
40	10287.5
41	14058.5
42	17347.0
43	19187.5
44	20637.5
45	18725.5
46	17204.0
47	16104.0
48	14335.0
49	12924.0
50	12182.0
51	11643.0
52	10016.5
53	8316.0
54	7703.0
55	7251.5
56	6259.0
57	5155.5
58	4593.0
59	4178.5
60	3504.0
61	3096.0
62	2948.0
63	2739.0
64	2344.5
65	2077.5
66	1951.5
67	1907.0
68	1791.0
69	1657.5
70	1539.0
71	1438.0
72	1359.5
73	1223.0
74	1061.5
75	958.0
76	919.0
77	836.0
78	712.5
79	633.0
80	586.0
81	478.0
82	374.0
83	331.0
84	268.0
85	195.5
86	133.5
87	81.0
88	65.0
89	45.5
90	24.5
91	8.0
92	9.0
93	6.0
94	1.5
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05385981977057325
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010048473837793515
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.009694767558703E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01266107703561983
52-53	0.026527970931774884
54-55	0.04481619331655908
56-57	0.052453033433282156
58-59	0.03094929942040403
60-61	0.030145421513380545
62-63	0.026126031978263138
64-65	0.016479497093981367
66-67	0.03054736046689229
68-69	0.009244595930770035
70-71	0.01266107703561983
72-73	0.019092100291807683
74-75	0.009244595930770035
76	0.0128620465123757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.53520583293809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.17195310369899	9.598302209860368
2	17.16350086340089	9.108740564483066
3	14.882304825956558	11.847150654758556
4	10.993971341149383	11.669091698352855
5	7.1950074222181835	9.546050145903841
6	4.273077039595262	6.803218727139722
7	2.384198248962404	4.428563389792358
8	1.4768699445605744	3.135123837391577
9	0.9239904268532825	2.2066448547794564
>10	4.448786694537853	20.095339919772982
>50	0.06513375140113302	1.0836274186676529
>100	0.016662122451452634	0.9968086047091167
>500	0.0	0.0
>1k	0.0030294768093550245	1.6989959564941277
>5k	0.0	0.0
>10k+	0.0015147384046775123	7.782342017894322
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	19362	7.782342017894322	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	2684	1.0788041512255118	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1543	0.6201918052686158	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	445	0.1788628343127246	RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	406	0.1631872151257667	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	379	0.1523348633809497	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	334	0.13424761047292139	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.0193895351174066E-4	0.0
2	0.0	0.0	0.0	4.0193895351174066E-4	0.0
3	0.0	0.0	0.0	4.0193895351174066E-4	0.0
4	0.0	0.0	0.0	0.0012058168605352218	0.0
5	0.0	0.0	0.0	0.0028135726745821842	0.0
6	0.0	0.0	0.0	0.0028135726745821842	0.0
7	0.0	0.0	0.0	0.003617450581605666	0.0
8	0.0	0.0	0.0	0.004421328488629147	0.0
9	0.0	0.0	0.0	0.0056271453491643685	0.0
10	0.0	0.0	0.0	0.008038779070234812	0.0
11	0.0	0.0	0.0	0.008440718023746553	0.0
12	0.0	0.0	0.0	0.008440718023746553	0.0
13	0.0	0.0	0.0	0.008842656977258294	0.0
14	0.0	0.0	0.0	0.008842656977258294	0.0
15	0.0	0.0	0.0	0.008842656977258294	0.0
16	0.0	0.0	0.0	0.008842656977258294	0.0
17	0.0	0.0	0.0	0.009244595930770035	0.0
18	0.0	0.0	0.0	0.010450412791305257	0.0
19	0.0	0.0	0.0	0.012862046512375701	0.0
20	0.0	0.0	0.0	0.015273680233446144	0.0
21	0.0	0.0	0.0	0.016077558140469624	0.0
22	0.0	0.0	0.0	0.01889113081505181	0.0
23	0.0	0.0	0.0	0.021704703489633995	0.0
24	0.0	0.0	0.0	0.028135726745821845	0.0
25	0.0	0.0	0.0	0.03376287209498621	0.0
26	0.0	0.0	0.0	0.036576444769568395	0.0
27	0.0	0.0	0.0	0.07033931686455461	0.0
28	0.0	0.0	0.0	0.11254290698328738	0.0
29	0.0	0.0	0.0	0.1591678255906493	0.0
30	0.0	0.0	0.0	0.2106160116401521	0.0
31	0.0	0.0	0.0	0.2729165494344719	0.0
32	0.0	0.0	0.0	0.33079575874016254	0.0
33	0.0	0.0	0.0	0.40033119769769365	0.0
34	0.0	0.0	0.0	0.49920818026158187	0.0
35	0.0	0.0	0.0	0.646317837246879	0.0
36	0.0	0.0	0.0	0.9316944942402148	0.0
37	0.0	0.0	0.0	1.3991494971743692	0.0
38	0.0	0.0	0.0	2.035820799536966	0.0
39	0.0	0.0	0.0	2.731979067019301	0.0
40	0.0	0.0	0.0	3.600167206604661	0.0
41	0.0	0.0	0.0	4.611847552593712	0.0
42	0.0	0.0	0.0	5.6311647386994865	0.0
43	0.0	0.0	0.0	6.68746030852834	0.0
44	0.0	0.0	0.0	7.690699936493646	0.0
45	0.0	0.0	0.0	8.802061142953608	0.0
46	0.0	0.0	0.0	9.798869747662724	0.0
47	0.0	0.0	0.0	10.847126538421344	0.0
48	0.0	0.0	0.0	11.730990297193662	0.0
49	0.0	0.0	0.0	12.622490896082702	0.0
50	0.0	0.0	0.0	13.511177922297161	0.0
51	0.0	0.0	0.0	14.452117012468147	0.0
52	0.0	0.0	0.0	15.388634774150502	0.0
53	0.0	0.0	0.0	16.329573864321485	0.0
54	0.0	0.0	0.0	17.24921018995635	0.0
55	0.0	0.0	0.0	18.078812190004584	0.0
56	0.0	0.0	0.0	18.885101730749135	0.0
57	0.0	0.0	0.0	19.679333102888332	0.0
58	0.0	0.0	0.0	20.438997725025523	0.0
59	0.0	0.0	0.0	21.12832303029816	0.0
60	0.0	0.0	0.0	21.789914547778483	0.0
61	0.0	0.0	0.0	22.499336800726706	0.0
62	0.0	0.0	0.0	23.126763507158532	0.0
63	0.0	0.0	0.0	23.820108201966285	0.0
64	0.0	0.0	0.0	24.480091963632564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGA	15	0.0022249017	69.98371	30
ATCGTAT	35	2.97121E-7	59.986046	39
AACGTAT	20	0.0069520585	52.48779	38
TGCATCG	20	0.0069520585	52.48779	16
AGTCGAG	20	0.0069520585	52.48779	29
CGTCTTC	2840	0.0	51.769333	48
CCGTCTT	2830	0.0	51.704876	47
ATCTCGT	2705	0.0	51.61461	37
TCGTATG	2895	0.0	51.611485	40
TCTCGTA	2700	0.0	51.58059	38
TATGCCG	2855	0.0	51.476643	43
GCCGTCT	2845	0.0	51.43227	46
CGTATGC	2900	0.0	51.281174	41
CTCGTAT	2885	0.0	51.062645	39
TGCCGTC	2875	0.0	50.99683	45
GATCTCG	2720	0.0	50.94403	36
ATGCCGT	2880	0.0	50.908295	44
CGGATCT	2725	0.0	50.722145	34
GTATGCC	2910	0.0	50.62396	42
CTTGAAA	2845	0.0	50.34512	57
>>END_MODULE