FastQCFastQC Report
Wed 25 May 2016
SRR1779794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399005
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGT200.006951630652.49445743
GCCGTAT551.3082354E-744.55191854
TCTCGGT1050.039.99577343
TGGTCGC1150.039.57095749
CGCCGTA1007.2759576E-1238.50558553
CGGTGGT1100.038.1825746
GTAGATC2050.037.56171838
GGTCGCC1151.8189894E-1236.5270450
TCGCCGT806.225673E-835.00507752
ACGTGTA601.2133531E-535.00507734
CTCGGTG1102.3646862E-1134.99630444
GTTCGAT400.002418799834.99630443
TCGGTGG1201.8189894E-1234.99630445
GATCTCG1550.033.8673941
TAGATCT2350.032.76660539
ACGCTTC552.9940318E-431.81083515
TCGATCT450.004304707531.11172339
CGGTCAA450.00430734931.1078249
ATCTCGG1357.2759576E-1231.10782242
TGCCGCT901.7573984E-731.10392613