##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779794_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399005
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.112204107717947	34.0	31.0	34.0	12.0	34.0
2	30.88249771306124	34.0	32.0	34.0	21.0	34.0
3	30.48788611671533	34.0	32.0	34.0	21.0	34.0
4	30.66738762672147	34.0	32.0	34.0	21.0	34.0
5	31.358296261951605	34.0	33.0	34.0	23.0	34.0
6	34.06451297602787	38.0	34.0	38.0	22.0	38.0
7	33.854169747246274	38.0	34.0	38.0	21.0	38.0
8	33.46964073131916	38.0	34.0	38.0	21.0	38.0
9	34.34929386849789	38.0	36.0	38.0	23.0	38.0
10-11	32.94261726043533	37.0	32.0	38.0	16.5	38.0
12-13	33.89191238205035	38.0	34.0	38.0	21.0	38.0
14-15	32.67354795052694	37.0	32.5	38.0	16.0	38.0
16-17	33.34193807095149	37.5	32.5	38.0	16.5	38.0
18-19	34.25250184834776	38.0	35.0	38.0	22.5	38.0
20-21	32.918026089898625	37.5	31.0	38.0	16.5	38.0
22-23	33.83494818360672	38.0	35.0	38.0	22.0	38.0
24-25	34.13970125687648	38.0	35.0	38.0	22.5	38.0
26-27	33.04530770291099	38.0	33.5	38.0	16.0	38.0
28-29	34.30900740592223	38.0	35.0	38.0	23.0	38.0
30-31	34.684897432363	38.0	36.5	38.0	24.0	38.0
32-33	34.79600130324181	38.0	37.0	38.0	25.5	38.0
34-35	34.71552737434368	38.0	36.5	38.0	25.0	38.0
36-37	34.23502587686871	38.0	35.5	38.0	22.5	38.0
38-39	34.187064071878794	38.0	35.0	38.0	22.0	38.0
40-41	34.21324795428629	38.0	35.5	38.0	22.5	38.0
42-43	34.57011566271099	38.0	36.5	38.0	24.5	38.0
44-45	34.607139008283106	38.0	36.0	38.0	24.0	38.0
46-47	34.52201852107117	38.0	36.0	38.0	23.5	38.0
48-49	34.274787283367374	38.0	35.5	38.0	22.5	38.0
50-51	33.663739802759366	38.0	35.0	38.0	17.0	38.0
52-53	34.182831042217515	38.0	35.0	38.0	23.0	38.0
54-55	34.337391762008	38.0	35.5	38.0	23.5	38.0
56-57	34.37868949010664	38.0	36.0	38.0	23.5	38.0
58-59	34.633916868209674	38.0	36.0	38.0	24.0	38.0
60-61	34.49522812997331	38.0	36.0	38.0	23.5	38.0
62-63	34.52983546572098	38.0	36.0	38.0	24.0	38.0
64-65	34.524680141852855	38.0	36.0	38.0	23.5	38.0
66-67	34.3556471723412	38.0	36.0	38.0	23.0	38.0
68-69	33.7706545030764	38.0	34.0	38.0	21.5	38.0
70-71	33.86007067580607	38.0	34.0	38.0	21.5	38.0
72-73	34.09709026202679	38.0	35.0	38.0	23.0	38.0
74-75	34.20012781794715	38.0	35.5	38.0	23.0	38.0
76	33.29884838535858	37.0	33.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	34.0
13	475.0
14	1848.0
15	3454.0
16	4690.0
17	5447.0
18	5469.0
19	4970.0
20	4140.0
21	3205.0
22	2760.0
23	2605.0
24	2819.0
25	3083.0
26	3622.0
27	4275.0
28	5369.0
29	6416.0
30	8049.0
31	10163.0
32	13108.0
33	17649.0
34	24590.0
35	37452.0
36	68754.0
37	154558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.378322021834364	23.725075437840225	11.677577167146195	27.219025373179218
2	18.1737070964023	25.54178519066177	33.3083545318981	22.97615318103783
3	18.423921624649438	24.110094410317718	32.23860472227026	25.227379242762588
4	13.404343303968622	20.46465583138056	33.99982456360196	32.13117630104886
5	13.67526722722773	34.51485570356262	35.419105023746575	16.39077204546309
6	27.25110465597141	39.587160677004356	17.694295388640903	15.46743927838333
7	24.136790182868143	29.811316971446907	22.468268768046197	23.583624077638753
8	21.49296600675203	36.93366517039232	22.046863212453413	19.52650561040224
9	23.2079165235874	17.73096753333016	22.60886347856819	36.45225246451425
10-11	23.302848177407064	26.25829590568232	26.051274211011748	24.387581705898864
12-13	22.725404443553337	24.250823924512225	27.25554817608802	25.768223455846417
14-15	23.082282589353966	26.16032962158179	24.33678443520475	26.420603353859494
16-17	23.444944298943625	27.85541534567236	24.90520168920189	23.794438666182128
18-19	21.503456192323693	27.53224359263548	26.947272390061006	24.017027824979824
20-21	23.357727346775103	26.6881367401411	26.35919349381587	23.594942419267927
22-23	23.696263761051135	26.88776245465197	25.503292627146784	23.912681157150107
24-25	22.0536026456704	28.278672603301075	25.625984513454547	24.041740237573983
26-27	22.382719886115858	28.262389662207205	26.207638056952092	23.147252394724838
28-29	21.807342760753727	28.032247392086784	26.844838562527258	23.315571284632234
30-31	22.40772625769731	27.51019415894116	26.734260143407447	23.347819439954083
32-33	21.544520856933765	28.228288438211145	26.78093452697216	23.44625617788293
34-35	21.48682551736756	28.22996007428739	26.97692429152537	23.306290116819678
36-37	22.727016352923336	27.501413192385932	26.405578067755524	23.365992386935208
38-39	22.352867455383997	26.918989372368156	27.63610387006216	23.092039302185682
40-41	21.706336575597554	27.50256265021892	27.16020761852727	23.630893155656253
42-43	20.80974835871133	27.10184573703542	27.756356195042365	24.33204970921089
44-45	20.492252166448402	26.39652115067703	28.604512729255045	24.506713953619517
46-47	20.766274306351825	27.073738926880125	28.253702109422285	23.906284657345758
48-49	20.557515303788822	26.93178614169084	28.443054868077265	24.06764368644307
50-51	21.938342663012488	26.872859468339865	27.543919668266938	23.644878200380706
52-53	19.487251471369703	27.626783440448378	28.27386025246398	24.612104835717936
54-55	19.924530288143412	26.973967837019508	28.0187884742636	25.082713400573482
56-57	20.198650181417122	27.363592497665707	27.61137256622195	24.826384754695223
58-59	19.93813745616608	27.115495965649743	27.98879054716267	24.957576031021507
60-61	19.52052605256264	26.995165400462025	27.744233765484942	25.740074781490392
62-63	20.013886086467206	27.059696385983074	27.387799042762378	25.53861848478734
64-65	20.45062852601906	26.611299054046096	27.175211420134143	25.762860999800708
66-67	19.945979143687957	27.731124034894215	26.345006517597515	25.977890303820317
68-69	20.258343598489738	28.549874875784298	25.89288063889164	25.298900886834325
70-71	20.318544370022746	28.513605804474913	26.01399740603129	25.153852419471058
72-73	20.630552391264303	28.673491510300554	25.99418271682914	24.701773381606007
74-75	21.333136154741748	28.800648684146214	25.520758268654998	24.34545689245704
76	21.081335779522192	29.430208427321126	25.059149648610983	24.429306144545702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	1.0
7	2.0
8	2.0
9	2.0
10	5.0
11	8.0
12	8.0
13	15.0
14	41.0
15	104.0
16	217.0
17	286.0
18	363.0
19	602.5
20	915.0
21	1065.0
22	1278.5
23	1799.0
24	2448.5
25	2791.0
26	3275.0
27	4226.5
28	5245.5
29	5797.0
30	6504.0
31	7793.0
32	9091.5
33	9808.0
34	10771.5
35	12601.5
36	14148.5
37	14829.0
38	15849.0
39	17495.5
40	18612.5
41	19121.0
42	19139.0
43	19490.5
44	20072.0
45	19972.5
46	19643.0
47	19435.5
48	18571.5
49	17465.0
50	17015.0
51	16505.5
52	15226.5
53	14060.5
54	13664.0
55	13181.5
56	11646.5
57	9890.0
58	9186.0
59	8483.0
60	7178.0
61	5918.5
62	5261.0
63	4787.0
64	4030.5
65	3370.5
66	2648.5
67	2304.0
68	2118.0
69	1836.0
70	1616.0
71	1492.0
72	1360.5
73	1122.0
74	985.5
75	956.0
76	942.0
77	847.5
78	707.5
79	648.0
80	634.5
81	541.0
82	415.5
83	370.0
84	355.0
85	291.5
86	222.0
87	201.0
88	170.5
89	124.5
90	88.0
91	48.5
92	30.0
93	31.5
94	24.5
95	14.0
96	12.0
97	8.5
98	8.0
99	13.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.506234257716069E-4
2	0.0
3	5.012468515432138E-4
4	0.0
5	0.0
6	0.0030074811092592824
7	0.0072680793473766
8	0.0035087279608024963
9	0.010526183882407489
10-11	0.0032581045350308893
12-13	0.0
14-15	5.012468515432138E-4
16-17	0.0
18-19	0.002756857683487676
20-21	0.0
22-23	0.0018796756932870516
24-25	0.003383416247916693
26-27	7.518702773148206E-4
28-29	0.006766832495833386
30-31	5.012468515432138E-4
32-33	2.506234257716069E-4
34-35	0.0035087279608024963
36-37	0.021428302903472387
38-39	0.01127805415972231
40-41	0.0010024937030864275
42-43	0.001127805415972231
44-45	0.005639027079861155
46-47	0.0023809225448302653
48-49	6.265585644290172E-4
50-51	0.0023809225448302653
52-53	0.01428553526898159
54-55	0.009273066753549455
56-57	0.015663964110725428
58-59	0.014034911843209985
60-61	0.02744326512199095
62-63	0.011152742446836506
64-65	0.023934537161188457
66-67	0.021302991190586585
68-69	3.759351386574103E-4
70-71	6.265585644290172E-4
72-73	0.005388403654089548
74-75	0.010902119021064898
76	0.004260598238117317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	399005.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.13124281220196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53891954800035	46.42603386131795
2	11.626424747731175	14.679812875736214
3	6.813112249104372	12.903607311149868
4	3.8753783634441126	9.786298098069764
5	2.060671649903705	6.504638114314583
6	1.0094280433837597	3.8235868144983645
7	0.4826442238061799	2.1328950779510283
8	0.26131581949287175	1.3197753960859222
9	0.11497493741140115	0.6532659621933201
>10	0.21713041772210484	1.7700864886829537
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.506234257716069E-4	0.0
2	0.0	0.0	0.0	7.518702773148206E-4	0.0
3	0.0	0.0	0.0	0.0010024937030864275	0.0
4	0.0	0.0	0.0	0.0010024937030864275	0.0
5	0.0	0.0	0.0	0.0032581045350308893	0.0
6	0.0	0.0	0.0	0.0032581045350308893	0.0
7	0.0	0.0	0.0	0.0032581045350308893	0.0
8	0.0	0.0	0.0	0.0032581045350308893	0.0
9	0.0	0.0	0.0	0.009273066753549454	0.0
10	0.0	0.0	0.0	0.01278179471435195	0.0
11	0.0	0.0	0.0	0.013032418140123557	0.0
12	0.0	0.0	0.0	0.013032418140123557	0.0
13	0.0	0.0	0.0	0.013283041565895165	0.0
14	0.0	0.0	0.0	0.014034911843209985	0.0
15	0.0	0.0	0.0	0.015538652397839626	0.0
16	0.0	0.0	0.0	0.016290522675154448	0.0
17	0.0	0.0	0.0	0.017293016378240874	0.0
18	0.0	0.0	0.0	0.018044886655555696	0.0
19	0.0	0.0	0.0	0.0182955100813273	0.0
20	0.0	0.0	0.0	0.019047380358642122	0.0
21	0.0	0.0	0.0	0.020300497487500156	0.0
22	0.0	0.0	0.0	0.0255635894287039	0.0
23	0.0	0.0	0.0	0.026315459706018722	0.0
24	0.0	0.0	0.0	0.033332915627623715	0.0
25	0.0	0.0	0.0	0.034335409330710144	0.0
26	0.0	0.0	0.0	0.036340396736882996	0.0
27	0.0	0.0	0.0	0.04937281487700655	0.0
28	0.0	0.0	0.0	0.06716707810679064	0.0
29	0.0	0.0	0.0	0.08821944587160561	0.0
30	0.0	0.0	0.0	0.11453490557762434	0.0
31	0.0	0.0	0.0	0.14536158694753198	0.0
32	0.0	0.0	0.0	0.1932306612699089	0.0
33	0.0	0.0	0.0	0.2481171915138908	0.0
34	0.0	0.0	0.0	0.3491184320998484	0.0
35	0.0	0.0	0.0	0.48295134146188645	0.0
36	0.0	0.0	0.0	0.6919712785554065	0.0
37	0.0	0.0	0.0	0.9967293642936805	0.0
38	0.0	0.0	0.0	1.3380784701946091	0.0
39	0.0	0.0	0.0	1.7007305672861242	0.0
40	0.0	0.0	0.0	2.156113331913134	0.0
41	0.0	0.0	0.0	2.691444969361286	0.0
42	0.0	0.0	0.0	3.24106214207842	0.0
43	0.0	0.0	0.0	3.80872420145111	0.0
44	0.0	0.0	0.0	4.415984762095713	0.0
45	0.0	0.0	0.0	5.101690455006829	0.0
46	0.0	0.0	0.0	5.7831355496798285	0.0
47	0.0	0.0	0.0	6.404180398741871	0.0
48	0.0	0.0	0.0	6.964574378767184	0.0
49	0.0	0.0	0.0	7.548276337389256	0.0
50	0.0	0.0	0.0	8.132980789714415	0.0
51	0.0	0.0	0.0	8.677334870490345	0.0
52	0.0	0.0	0.0	9.310660267415196	0.0
53	0.0	0.0	0.0	9.91817145148557	0.0
54	0.0	0.0	0.0	10.549241237578476	0.0
55	0.0	0.0	0.0	11.159509279332338	0.0
56	0.0	0.0	0.0	11.796343404217993	0.0
57	0.0	0.0	0.0	12.367263568125713	0.0
58	0.0	0.0	0.0	12.890815904562599	0.0
59	0.0	0.0	0.0	13.368504154083283	0.0
60	0.0	0.0	0.0	13.877770955251187	0.0
61	0.0	0.0	0.0	14.334657460432826	0.0
62	0.0	0.0	0.0	14.831643713737924	0.0
63	0.0	0.0	0.0	15.350434205085149	0.0
64	0.0	0.0	0.0	15.854688537737623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGGT	20	0.0069516306	52.494457	43
GCCGTAT	55	1.3082354E-7	44.551918	54
TCTCGGT	105	0.0	39.995773	43
TGGTCGC	115	0.0	39.570957	49
CGCCGTA	100	7.2759576E-12	38.505585	53
CGGTGGT	110	0.0	38.18257	46
GTAGATC	205	0.0	37.561718	38
GGTCGCC	115	1.8189894E-12	36.52704	50
TCGCCGT	80	6.225673E-8	35.005077	52
ACGTGTA	60	1.2133531E-5	35.005077	34
CTCGGTG	110	2.3646862E-11	34.996304	44
GTTCGAT	40	0.0024187998	34.996304	43
TCGGTGG	120	1.8189894E-12	34.996304	45
GATCTCG	155	0.0	33.86739	41
TAGATCT	235	0.0	32.766605	39
ACGCTTC	55	2.9940318E-4	31.810835	15
TCGATCT	45	0.0043047075	31.111723	39
CGGTCAA	45	0.004307349	31.107824	9
ATCTCGG	135	7.2759576E-12	31.107822	42
TGCCGCT	90	1.7573984E-7	31.103926	13
>>END_MODULE