FastQCFastQC Report
Wed 25 May 2016
SRR1779794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399005
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT247696.207691632936931No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG33230.8328216438390497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC18700.4686658061929049No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT5380.13483540306512448Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4220.1057630856756181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG200.006925427452.544652
TAACCGG200.00694262552.5116647
ATGCCGT34300.049.1699444
TCTCGTA33450.049.16414338
TATGCCG34500.049.08773443
CGTATGC34650.049.07719441
GTATGCC34600.048.9458642
ATCTCGT33600.048.9446637
TCGTATG34750.048.9359740
TATCTCG33500.048.88186636
CTCGTAT34500.048.78347439
TGCCGTC34600.048.7558345
CCGTCTT34700.048.42573547
GCCGTCT34800.048.38718446
CGTCTTC34750.048.16665348
TTATCTC34550.047.49758535
CTGCTTG35550.047.49460254
TGCTTGA35300.047.32885455
GATTATC34550.047.29503633
TCTGCTT36100.046.77153
Produced by FastQC (version 0.11.5)