##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779794_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399005
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.62091953734915	34.0	34.0	34.0	34.0	34.0
2	33.71020162654603	34.0	34.0	34.0	34.0	34.0
3	33.78885477625594	34.0	34.0	34.0	34.0	34.0
4	33.65293667999148	34.0	34.0	34.0	34.0	34.0
5	33.67737998270698	34.0	34.0	34.0	34.0	34.0
6	37.39421811756745	38.0	38.0	38.0	37.0	38.0
7	37.45946542023283	38.0	38.0	38.0	37.0	38.0
8	37.369754764977884	38.0	38.0	38.0	37.0	38.0
9	37.28597636621095	38.0	38.0	38.0	37.0	38.0
10-11	37.25912081302239	38.0	38.0	38.0	36.5	38.0
12-13	37.38234608588865	38.0	38.0	38.0	37.0	38.0
14-15	37.39491861004248	38.0	38.0	38.0	37.0	38.0
16-17	37.36084635530883	38.0	38.0	38.0	37.0	38.0
18-19	37.25505820729064	38.0	38.0	38.0	36.5	38.0
20-21	37.249009410909636	38.0	38.0	38.0	36.5	38.0
22-23	37.235078507788124	38.0	38.0	38.0	36.0	38.0
24-25	37.39716168970314	38.0	38.0	38.0	37.0	38.0
26-27	37.32715630129948	38.0	38.0	38.0	37.0	38.0
28-29	36.947013195323365	38.0	38.0	38.0	36.0	38.0
30-31	37.199199258154664	38.0	38.0	38.0	36.0	38.0
32-33	37.26470595606571	38.0	38.0	38.0	37.0	38.0
34-35	37.25965464091929	38.0	38.0	38.0	37.0	38.0
36-37	37.24052455483014	38.0	38.0	38.0	36.5	38.0
38-39	36.990877307301915	38.0	38.0	38.0	36.0	38.0
40-41	37.119176451422916	38.0	38.0	38.0	36.0	38.0
42-43	37.31581183193193	38.0	38.0	38.0	37.0	38.0
44-45	37.13586295911079	38.0	38.0	38.0	36.0	38.0
46-47	37.23262866380121	38.0	38.0	38.0	36.5	38.0
48-49	36.95202441072167	38.0	38.0	38.0	35.5	38.0
50-51	37.10481322289194	38.0	38.0	38.0	36.0	38.0
52-53	37.03362489191865	38.0	38.0	38.0	36.0	38.0
54-55	37.01816518589993	38.0	38.0	38.0	36.0	38.0
56-57	36.920162654603324	38.0	38.0	38.0	36.0	38.0
58-59	37.00677685743286	38.0	38.0	38.0	36.0	38.0
60-61	36.75838022079924	38.0	37.5	38.0	35.0	38.0
62-63	36.61541835315347	38.0	37.0	38.0	34.0	38.0
64-65	36.88876831117405	38.0	37.5	38.0	35.5	38.0
66-67	36.734164985401186	38.0	37.5	38.0	35.0	38.0
68-69	36.83591433691307	38.0	38.0	38.0	35.0	38.0
70-71	36.38179346123482	38.0	37.0	38.0	33.5	38.0
72-73	35.52912745454317	38.0	37.0	38.0	29.0	38.0
74-75	35.41014147692385	38.0	37.0	38.0	26.0	38.0
76	35.16490269545494	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	5.0
22	20.0
23	34.0
24	76.0
25	142.0
26	293.0
27	537.0
28	832.0
29	1491.0
30	2347.0
31	3934.0
32	6212.0
33	9834.0
34	17124.0
35	34027.0
36	57627.0
37	264469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.16304658545514	19.979989668851587	12.51824250114094	30.338721244552325
2	17.89777070462776	20.973922632548465	38.88347263818749	22.244834024636283
3	18.44989411160261	22.23255347677347	34.96948659791231	24.34806581371161
4	13.13291813385797	22.819012293079034	35.732133682535306	28.315935890527687
5	13.167504166614453	32.94845929249007	39.66291149233719	14.221125048558289
6	27.304920990965027	41.02504981140587	18.079222064886405	13.590807132742697
7	23.986165586897407	29.293116627611184	22.670392601596472	24.050325183894937
8	21.608751770027943	39.843360358892745	20.607761807496146	17.940126063583165
9	22.168393879775945	16.649415420859388	21.745842783925013	39.436347915439654
10-11	22.931166276111828	28.13874512850716	28.69024197691758	20.239846618463428
12-13	22.24809212917131	22.434931893084045	29.366048044510723	25.95092793323392
14-15	20.714652698587738	23.55509329457024	23.656094535156203	32.07415947168582
16-17	24.4602197967444	29.45000689214421	23.85496422350597	22.234809087605417
18-19	24.498815804313228	26.153556972970264	25.38953145950552	23.958095763210988
20-21	21.151614641420533	25.183268380095488	24.745053320133835	28.92006365835015
22-23	20.77818573702084	29.062292452475535	24.88690617912056	25.272615631383065
24-25	24.57512637812938	28.928749552006135	24.895802266159734	21.60032180370475
26-27	24.761469154521873	29.07883359857646	25.176877482738313	20.982819764163356
28-29	20.712898334607335	29.318053658475456	25.444292678036618	24.52475532888059
30-31	24.595807070086842	26.28952018145136	28.217315572486562	20.89735717597524
32-33	23.953960476685758	28.927707672836178	26.270096865954063	20.848234984524
34-35	21.077555419105025	28.96505056327615	29.124822997205545	20.83257102041328
36-37	20.40137341637323	28.92695580255886	29.92042706231752	20.75124371875039
38-39	20.21440834074761	25.67499154145938	29.911028683851082	24.19957143394193
40-41	20.159772433929398	24.246312702848336	30.365408954775003	25.228505908447264
42-43	23.903734939004142	24.153106810107705	30.034899530704696	21.908258720183458
44-45	20.188970063031793	27.915940902996205	30.552875277252166	21.34221375671984
46-47	23.506472349970554	24.54417864437789	27.568451523163873	24.380897482487686
48-49	22.847583363616998	24.23265372614378	27.10517412062505	25.814588789614167
50-51	19.91547516393607	24.311543706258647	30.69264242886077	25.08033870094451
52-53	18.696597268187016	24.38825039613294	34.59744870329141	22.31770363238863
54-55	18.442559231247618	24.602157602262924	31.33250245752001	25.622780708969444
56-57	22.183602791633124	24.36033433392767	27.732888959106035	25.72317391533317
58-59	18.60341505547804	24.41936409916974	34.091384888862144	22.885835956490073
60-61	22.289079599557738	27.931549745649463	27.26903492211533	22.51033573267746
62-63	18.663521105927007	32.65570901724627	25.960277685154775	22.720492191671955
64-65	18.425993467542007	33.10033564701666	25.294474483055968	23.179196402385376
66-67	18.349944212518963	33.416826507202224	24.942645454887362	23.290583825391455
68-69	18.564508750932116	33.28148988300759	24.666662489112248	23.487338876948048
70-71	18.927766623302077	32.61079903645383	24.615671913030184	23.845762427213913
72-73	19.04455109845413	31.220113235974296	24.96694046023893	24.768395205332645
74-75	19.637467915296384	30.73576742600465	24.761495347717975	24.865269310980988
76	19.547355017245525	31.312164113259005	24.676646346354374	24.46383452314109
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.0
10	5.5
11	15.0
12	22.0
13	23.0
14	65.5
15	137.5
16	234.0
17	300.0
18	399.0
19	643.5
20	973.5
21	1158.0
22	1444.5
23	2008.5
24	2694.0
25	3102.0
26	3395.0
27	4249.5
28	5333.5
29	5856.0
30	6480.5
31	7793.5
32	9468.0
33	10454.0
34	11022.0
35	12142.0
36	13573.5
37	14453.0
38	19090.0
39	25804.5
40	26964.0
41	24019.5
42	21993.0
43	20685.5
44	18741.5
45	17621.0
46	17137.0
47	16817.5
48	15871.5
49	14820.5
50	14396.0
51	14189.5
52	13181.0
53	11841.0
54	11303.0
55	11047.0
56	10368.0
57	9104.0
58	8263.0
59	7581.0
60	6446.5
61	5498.5
62	5003.0
63	4436.5
64	3471.5
65	2932.0
66	2513.0
67	2235.0
68	2102.5
69	1769.5
70	1584.5
71	1600.0
72	1363.0
73	1078.5
74	1009.0
75	987.0
76	897.0
77	744.5
78	677.5
79	673.0
80	607.5
81	506.0
82	407.0
83	344.0
84	294.5
85	259.5
86	205.5
87	137.0
88	122.5
89	92.5
90	54.5
91	33.5
92	35.0
93	23.0
94	13.0
95	9.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05288154283780905
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010024937030864275
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.265585644290172E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017293016378240874
52-53	0.03734289043996942
54-55	0.057392764501697974
56-57	0.06065086903672886
58-59	0.03847069585594165
60-61	0.0365910201626546
62-63	0.03408478590493853
64-65	0.01854613350709891
66-67	0.04260598238117317
68-69	0.011904612724151326
70-71	0.015538652397839628
72-73	0.025939524567361313
74-75	0.015288028972068018
76	0.015288028972068018
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	399005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.067366437139306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.10690398218591	11.257859544702207
2	19.79523972258022	12.695624117899913
3	15.514440995719077	14.925217934313
4	11.288475958086405	14.479667802591756
5	7.541462362957203	12.091741853242153
6	4.62090008302432	8.8908057739049
7	2.631449297243848	5.906855421282983
8	1.3797405375902227	3.5395716325664996
9	0.7512847578970032	2.1682551267108616
>10	1.3535398977848971	5.739821326187022
>50	0.0063094875927191775	0.1385951257064222
>100	0.007098173541809074	0.4522045342392024
>500	7.886859490898972E-4	0.13606601757309336
>1k	0.0015773718981797944	1.3133658536376835
>5k	0.0	0.0
>10k+	7.886859490898972E-4	6.264347935442284
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	24769	6.207691632936931	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3323	0.8328216438390497	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	1870	0.4686658061929049	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	538	0.13483540306512448	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	422	0.1057630856756181	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.012468515432138E-4	0.0
2	0.0	0.0	0.0	0.0010024937030864275	0.0
3	0.0	0.0	0.0	0.0010024937030864275	0.0
4	0.0	0.0	0.0	0.0010024937030864275	0.0
5	0.0	0.0	0.0	0.0035087279608024963	0.0
6	0.0	0.0	0.0	0.0035087279608024963	0.0
7	0.0	0.0	0.0	0.0035087279608024963	0.0
8	0.0	0.0	0.0	0.0035087279608024963	0.0
9	0.0	0.0	0.0	0.01127805415972231	0.0
10	0.0	0.0	0.0	0.015789275823611233	0.0
11	0.0	0.0	0.0	0.016290522675154448	0.0
12	0.0	0.0	0.0	0.016290522675154448	0.0
13	0.0	0.0	0.0	0.01679176952669766	0.0
14	0.0	0.0	0.0	0.01779426322978409	0.0
15	0.0	0.0	0.0	0.019548627210185337	0.0
16	0.0	0.0	0.0	0.020551120913271763	0.0
17	0.0	0.0	0.0	0.021302991190586585	0.0
18	0.0	0.0	0.0	0.02230548489367301	0.0
19	0.0	0.0	0.0	0.02255610831944462	0.0
20	0.0	0.0	0.0	0.023558602022531044	0.0
21	0.0	0.0	0.0	0.025312966002932293	0.0
22	0.0	0.0	0.0	0.02957356424104961	0.0
23	0.0	0.0	0.0	0.031578551647222466	0.0
24	0.0	0.0	0.0	0.03508727960802496	0.0
25	0.0	0.0	0.0	0.03608977331111139	0.0
26	0.0	0.0	0.0	0.03834538414305585	0.0
27	0.0	0.0	0.0	0.052630919412037444	0.0
28	0.0	0.0	0.0	0.07192892319645117	0.0
29	0.0	0.0	0.0	0.09548752521898222	0.0
30	0.0	0.0	0.0	0.12455984260848861	0.0
31	0.0	0.0	0.0	0.1558877708299395	0.0
32	0.0	0.0	0.0	0.20500996228117443	0.0
33	0.0	0.0	0.0	0.2659114547436749	0.0
34	0.0	0.0	0.0	0.3736795278254659	0.0
35	0.0	0.0	0.0	0.5152817633864237	0.0
36	0.0	0.0	0.0	0.742095963709728	0.0
37	0.0	0.0	0.0	1.0671545469355022	0.0
38	0.0	0.0	0.0	1.4202829538476962	0.0
39	0.0	0.0	0.0	1.7922081176927607	0.0
40	0.0	0.0	0.0	2.2741569654515605	0.0
41	0.0	0.0	0.0	2.8320447112191576	0.0
42	0.0	0.0	0.0	3.3964486660568163	0.0
43	0.0	0.0	0.0	3.982656858936605	0.0
44	0.0	0.0	0.0	4.609967293642937	0.0
45	0.0	0.0	0.0	5.323742810240473	0.0
46	0.0	0.0	0.0	6.044285159333843	0.0
47	0.0	0.0	0.0	6.686131752734928	0.0
48	0.0	0.0	0.0	7.27133745191163	0.0
49	0.0	0.0	0.0	7.8921316775479005	0.0
50	0.0	0.0	0.0	8.498139121063646	0.0
51	0.0	0.0	0.0	9.055024373128155	0.0
52	0.0	0.0	0.0	9.694865979123069	0.0
53	0.0	0.0	0.0	10.310647736243906	0.0
54	0.0	0.0	0.0	10.940715028633726	0.0
55	0.0	0.0	0.0	11.553489304645305	0.0
56	0.0	0.0	0.0	12.194834651194848	0.0
57	0.0	0.0	0.0	12.78329845490658	0.0
58	0.0	0.0	0.0	13.308354531898097	0.0
59	0.0	0.0	0.0	13.800578940113532	0.0
60	0.0	0.0	0.0	14.318617561183444	0.0
61	0.0	0.0	0.0	14.783022769138231	0.0
62	0.0	0.0	0.0	15.295046427989623	0.0
63	0.0	0.0	0.0	15.829124948308918	0.0
64	0.0	0.0	0.0	16.36169972807358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACCG	20	0.0069254274	52.5446	52
TAACCGG	20	0.006942625	52.51166	47
ATGCCGT	3430	0.0	49.16994	44
TCTCGTA	3345	0.0	49.164143	38
TATGCCG	3450	0.0	49.087734	43
CGTATGC	3465	0.0	49.077194	41
GTATGCC	3460	0.0	48.94586	42
ATCTCGT	3360	0.0	48.94466	37
TCGTATG	3475	0.0	48.93597	40
TATCTCG	3350	0.0	48.881866	36
CTCGTAT	3450	0.0	48.783474	39
TGCCGTC	3460	0.0	48.75583	45
CCGTCTT	3470	0.0	48.425735	47
GCCGTCT	3480	0.0	48.387184	46
CGTCTTC	3475	0.0	48.166653	48
TTATCTC	3455	0.0	47.497585	35
CTGCTTG	3555	0.0	47.494602	54
TGCTTGA	3530	0.0	47.328854	55
GATTATC	3455	0.0	47.295036	33
TCTGCTT	3610	0.0	46.771	53
>>END_MODULE