##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779793_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131087
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.919175814535386	34.0	23.0	34.0	12.0	34.0
2	29.261856629566623	34.0	27.0	34.0	12.0	34.0
3	29.07092236453653	34.0	27.0	34.0	11.0	34.0
4	29.18663177889493	34.0	24.0	34.0	12.0	34.0
5	29.784906207327957	34.0	31.0	34.0	11.0	34.0
6	32.188172740241214	37.0	31.0	38.0	12.0	38.0
7	32.484067832813324	37.0	31.0	38.0	12.0	38.0
8	31.714449182603918	37.0	27.0	38.0	11.0	38.0
9	32.558514574290356	37.0	31.0	38.0	12.0	38.0
10-11	31.216596611410743	37.0	27.0	38.0	11.0	38.0
12-13	32.02049020879264	37.0	29.5	38.0	11.0	38.0
14-15	30.86616903277976	37.0	25.5	38.0	11.0	38.0
16-17	31.442839488278775	37.0	28.5	38.0	11.0	38.0
18-19	32.20478766010359	37.0	31.0	38.0	11.0	38.0
20-21	31.08853662071754	37.0	27.0	38.0	11.0	38.0
22-23	31.896580133804264	37.0	29.0	38.0	11.0	38.0
24-25	32.189095791344684	37.0	31.0	38.0	11.0	38.0
26-27	31.136409407492735	37.0	26.5	38.0	11.0	38.0
28-29	32.23156758488638	37.5	31.5	38.0	11.0	38.0
30-31	32.58533645594147	38.0	32.5	38.0	11.0	38.0
32-33	32.72367587937782	38.0	34.0	38.0	11.0	38.0
34-35	32.66922730705562	38.0	33.0	38.0	11.0	38.0
36-37	32.2004699169254	37.5	29.0	38.0	11.0	38.0
38-39	32.12777010687559	37.5	29.0	38.0	11.0	38.0
40-41	32.139048875937355	37.0	30.0	38.0	11.0	38.0
42-43	32.485711779200074	37.5	32.5	38.0	11.0	38.0
44-45	32.51798805373531	38.0	32.0	38.0	11.0	38.0
46-47	32.43841876006012	38.0	31.5	38.0	11.0	38.0
48-49	32.18346594246569	37.5	30.0	38.0	11.0	38.0
50-51	31.583307269218153	37.0	28.5	38.0	11.0	38.0
52-53	32.12254456963696	37.0	31.0	38.0	11.0	38.0
54-55	32.246553815405036	37.0	31.5	38.0	11.0	38.0
56-57	32.273299411840995	37.5	31.0	38.0	11.0	38.0
58-59	32.47381891415625	38.0	32.0	38.0	11.0	38.0
60-61	32.39305575686376	38.0	31.0	38.0	11.0	38.0
62-63	32.3564655534111	38.0	31.0	38.0	11.0	38.0
64-65	32.37422856576167	38.0	31.5	38.0	11.0	38.0
66-67	32.30994682920503	38.0	31.0	38.0	11.0	38.0
68-69	31.7265556462502	37.0	28.0	38.0	11.0	38.0
70-71	31.834945494213766	37.0	29.0	38.0	11.0	38.0
72-73	32.0200286832409	37.0	31.0	38.0	11.0	38.0
74-75	32.07379068862664	37.0	31.0	38.0	11.0	38.0
76	31.163135932624897	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	17.0
13	281.0
14	1210.0
15	2429.0
16	3417.0
17	4074.0
18	4008.0
19	3451.0
20	2735.0
21	2030.0
22	1561.0
23	1329.0
24	1177.0
25	1301.0
26	1301.0
27	1477.0
28	1765.0
29	2096.0
30	2573.0
31	3214.0
32	3988.0
33	5327.0
34	7491.0
35	11273.0
36	20127.0
37	41435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.723097813649055	18.59313732969196	9.388493050363882	29.295271806295105
2	19.847887280966077	20.431469176958814	30.011366497059207	29.709277045015902
3	17.47923135017202	20.642016370807173	33.56091755856798	28.31783472045283
4	13.04782320138534	24.718698269088467	29.976275298084477	32.257203231441714
5	13.334655610396148	32.784334068214235	35.847948309138204	18.033062012251406
6	26.034451191602333	37.25778889872143	16.365841750328034	20.341918159348204
7	25.63816963945132	31.64070248249134	18.842216085079112	23.878911792978226
8	22.680876384608343	35.498611577309205	16.748039425101464	25.072472612980988
9	23.724365234375	17.870330810546875	18.41278076171875	39.992523193359375
10-11	22.34156984506	26.309284956859397	24.155687617768894	27.19345758031171
12-13	22.484685743056136	22.9008215917673	25.536094349554112	29.07839831562245
14-15	24.73023537892918	25.00715176620018	21.696360799929817	28.566252054940822
16-17	25.75617719529778	25.60475104320032	22.625813391106668	26.01325837039523
18-19	22.130000114430004	25.575106133829706	24.775621831719235	27.519271920021055
20-21	23.998565837954946	25.16000823880324	24.28616109911738	26.555264824124436
22-23	25.88940721443039	24.96328704003906	22.828023145375692	26.319282600154864
24-25	22.177720815977786	28.072122793365985	23.150012968981247	26.600143421674982
26-27	23.05937727666294	28.59317010653352	23.925987237337463	24.421465379466074
28-29	22.5937129277494	27.277547423871955	25.67697982506666	24.451759823311985
30-31	23.256692120500126	26.495380930221913	25.300373034702144	24.947553914575813
32-33	21.452628607827656	28.070014837530945	25.363786507382528	25.11357004725887
34-35	21.374325329468082	29.157973032250684	24.680640054927242	24.787061583353996
36-37	23.872853098938663	26.077170172667692	24.572908798193208	25.47706793020044
38-39	24.052260156399008	25.35037192447072	26.235361434293342	24.362006484836925
40-41	21.95216844032498	26.727695769920278	25.822557882290116	25.497577907464624
42-43	20.339551968386804	26.11712292452578	26.594677479030704	26.94864762805671
44-45	19.862146306482252	24.740427674491343	28.64106926251707	26.756356756509337
46-47	20.454822861676483	26.114933935491745	27.305391962405785	26.12485124042599
48-49	20.671925300947468	26.45438872190776	26.79004622919305	26.083639747951725
50-51	22.860325137508298	26.09033970842901	25.361030797281114	25.688304356781583
52-53	18.936847927673618	27.65339792862729	26.397985847527135	27.01176829617197
54-55	19.280798925814032	25.975388324330922	26.47509841618603	28.268714333669013
56-57	20.292287679437244	26.75183776546031	25.619614021568708	27.336260533533736
58-59	19.839480600727065	26.346467085512437	25.71438380463019	28.099668509130304
60-61	18.87306425952562	25.501604294396607	26.61793376038213	29.007397685695647
62-63	20.222927506599326	25.736988266170258	25.71371896791126	28.326365259319164
64-65	20.724793602832335	25.353278701033133	24.86990492758931	29.05202276854523
66-67	19.412123755388546	28.170373478808227	23.61538168084538	28.802121084957843
68-69	20.232441937194142	29.205524596354316	22.750626494719135	27.811406971732406
70-71	19.654960655750212	29.912691238228195	23.52835722976813	26.903990876253463
72-73	20.4124214694136	29.966928467075327	23.07912373788426	26.541526325626812
74-75	21.628539494714836	30.080984478292876	22.334245028590612	25.956230998401676
76	20.55736104118033	31.803756427274532	21.698631391037672	25.94025114050747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	1.5
12	1.0
13	2.0
14	4.5
15	4.0
16	13.0
17	24.0
18	26.5
19	50.5
20	70.5
21	69.0
22	79.0
23	128.5
24	228.5
25	289.0
26	320.0
27	437.0
28	614.5
29	706.0
30	856.5
31	1129.0
32	1554.0
33	1857.0
34	2068.5
35	2560.0
36	3150.0
37	3460.0
38	3877.0
39	4667.0
40	5428.0
41	6147.5
42	6479.0
43	6814.0
44	7491.5
45	7872.0
46	7910.0
47	8020.5
48	7940.5
49	7525.0
50	7300.0
51	6994.0
52	6259.0
53	5580.5
54	5331.0
55	4984.0
56	4309.5
57	3684.0
58	3386.0
59	3120.0
60	2585.0
61	2149.0
62	1982.0
63	1830.0
64	1513.0
65	1234.0
66	1091.0
67	1062.0
68	1031.5
69	911.0
70	762.0
71	703.0
72	646.5
73	541.0
74	466.5
75	441.0
76	422.0
77	388.0
78	331.5
79	290.0
80	262.0
81	218.5
82	167.5
83	132.0
84	122.5
85	106.0
86	80.5
87	62.0
88	58.0
89	48.0
90	32.5
91	22.5
92	22.0
93	19.5
94	11.5
95	5.0
96	4.0
97	6.5
98	6.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.628521516244937E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002288556454873481
7	0.006865669364620443
8	0.002288556454873481
9	0.011442782274367404
10-11	0.003051408606497975
12-13	0.0
14-15	3.8142607581224686E-4
16-17	0.0
18-19	0.001907130379061234
20-21	0.0
22-23	0.001907130379061234
24-25	0.003814260758122468
26-27	0.0011442782274367405
28-29	0.006484243288808196
30-31	0.0
32-33	3.8142607581224686E-4
34-35	0.0034328346823102214
36-37	0.019834155942236836
38-39	0.009154225819493924
40-41	0.0015257043032489875
42-43	0.001907130379061234
44-45	0.004577112909746962
46-47	0.002288556454873481
48-49	7.628521516244937E-4
50-51	0.003051408606497975
52-53	0.011061356198555158
54-55	0.00839137366786943
56-57	0.01334991265342864
58-59	0.00953565189530617
60-61	0.024792694927796042
62-63	0.009917077971118418
64-65	0.022122712397110316
66-67	0.01678274733573886
68-69	3.8142607581224686E-4
70-71	3.8142607581224686E-4
72-73	0.005721391137183702
74-75	0.00953565189530617
76	0.003814260758122468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	131087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.91148626484701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54885203799233	55.507411108653024
2	8.927978391303386	12.304805205703083
3	4.854207718024221	10.035320054620215
4	2.5859587752119912	7.128090504779269
5	1.2907653818053004	4.447428043970798
6	0.6608807315075165	2.732536407118936
7	0.3265658556025417	1.5752896931045794
8	0.21475856266743418	1.1839465393212143
9	0.14391037704518786	0.8925370174006575
>10	0.4450151659397348	4.140761479017751
>50	0.001107002900347599	0.05187394631046557
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004577112909746962	0.0
2	0.0	0.0	0.0	0.004577112909746962	0.0
3	0.0	0.0	0.0	0.004577112909746962	0.0
4	0.0	0.0	0.0	0.006102817212995949	0.0
5	0.0	0.0	0.0	0.006102817212995949	0.0
6	0.0	0.0	0.0	0.006102817212995949	0.0
7	0.0	0.0	0.0	0.006102817212995949	0.0
8	0.0	0.0	0.0	0.006102817212995949	0.0
9	0.0	0.0	0.0	0.009154225819493924	0.0
10	0.0	0.0	0.0	0.012968486577616392	0.0
11	0.0	0.0	0.0	0.016019895184114365	0.0
12	0.0	0.0	0.0	0.01907130379061234	0.0
13	0.0	0.0	0.0	0.019834155942236836	0.0
14	0.0	0.0	0.0	0.019834155942236836	0.0
15	0.0	0.0	0.0	0.024411268851983796	0.0
16	0.0	0.0	0.0	0.024411268851983796	0.0
17	0.0	0.0	0.0	0.024411268851983796	0.0
18	0.0	0.0	0.0	0.02669982530685728	0.0
19	0.0	0.0	0.0	0.030514086064979747	0.0
20	0.0	0.0	0.0	0.03203979036822873	0.0
21	0.0	0.0	0.0	0.03203979036822873	0.0
22	0.0	0.0	0.0	0.0366169032779757	0.0
23	0.0	0.0	0.0	0.0366169032779757	0.0
24	0.0	0.0	0.0	0.0366169032779757	0.0
25	0.0	0.0	0.0	0.038905459732849174	0.0
26	0.0	0.0	0.0	0.04119401618772266	0.0
27	0.0	0.0	0.0	0.07399665870757588	0.0
28	0.0	0.0	0.0	0.0968822232563107	0.0
29	0.0	0.0	0.0	0.12129349210829449	0.0
30	0.0	0.0	0.0	0.13807623944403336	0.0
31	0.0	0.0	0.0	0.17316743841876006	0.0
32	0.0	0.0	0.0	0.23343275839709507	0.0
33	0.0	0.0	0.0	0.2929352262238056	0.0
34	0.0	0.0	0.0	0.36769473708300593	0.0
35	0.0	0.0	0.0	0.44931991730682674	0.0
36	0.0	0.0	0.0	0.6545271460938156	0.0
37	0.0	0.0	0.0	0.9955220578699642	0.0
38	0.0	0.0	0.0	1.3548254212851008	0.0
39	0.0	0.0	0.0	1.7728684003753232	0.0
40	0.0	0.0	0.0	2.3297504710612036	0.0
41	0.0	0.0	0.0	2.9293522622380554	0.0
42	0.0	0.0	0.0	3.654824658432949	0.0
43	0.0	0.0	0.0	4.486333503703647	0.0
44	0.0	0.0	0.0	5.275885480634998	0.0
45	0.0	0.0	0.0	5.97389519937141	0.0
46	0.0	0.0	0.0	6.745901576815397	0.0
47	0.0	0.0	0.0	7.487393868194405	0.0
48	0.0	0.0	0.0	8.142683866439846	0.0
49	0.0	0.0	0.0	8.730080023190705	0.0
50	0.0	0.0	0.0	9.395287099407264	0.0
51	0.0	0.0	0.0	10.09558537459855	0.0
52	0.0	0.0	0.0	10.89200302089452	0.0
53	0.0	0.0	0.0	11.703677710222982	0.0
54	0.0	0.0	0.0	12.447458558056862	0.0
55	0.0	0.0	0.0	13.183610884374499	0.0
56	0.0	0.0	0.0	13.933494549421377	0.0
57	0.0	0.0	0.0	14.563610426663208	0.0
58	0.0	0.0	0.0	15.138038096836452	0.0
59	0.0	0.0	0.0	15.580492344778659	0.0
60	0.0	0.0	0.0	16.135085859009667	0.0
61	0.0	0.0	0.0	16.595085706439235	0.0
62	0.0	0.0	0.0	17.16112200294461	0.0
63	0.0	0.0	0.0	17.832431896374164	0.0
64	0.0	0.0	0.0	18.433559391854264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGG	15	0.0022204963	70.0	15
TGTCGAT	15	0.0022204963	70.0	37
GACGATC	25	2.3832014E-4	55.999996	23
CACGGCA	20	0.0069383685	52.500004	19
TCGGAGC	20	0.0069383685	52.500004	48
GTGTCGA	20	0.0069383685	52.500004	36
TCGTCGG	20	0.0069383685	52.500004	66
AAGGGTT	20	0.0069383685	52.500004	40
TGTGTCG	20	0.0069383685	52.500004	35
GTCGGTT	20	0.0069383685	52.500004	12
GTACGAA	20	0.0069383685	52.500004	43
GTATCAT	35	2.0772692E-5	50.000004	57
GGTCGGT	30	5.8602507E-4	46.666664	11
GTGTAGA	170	0.0	45.294117	36
CTATACA	105	0.0	43.333336	2
CCGACAA	60	2.576362E-7	40.833332	21
CGTATCA	35	0.0012517024	40.000004	56
CGGCCCG	35	0.0012517024	40.000004	54
GTAGATC	140	0.0	40.000004	38
TTACGCT	35	0.0012517024	40.000004	13
>>END_MODULE