FastQCFastQC Report
Wed 25 May 2016
SRR1779793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131087
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT1772913.524605796150649No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG23941.8262680509890379No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC12700.9688222325631068No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4270.3257378687436588RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3180.24258698421658897No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT2690.2052072287869888No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT2220.1693531776606376No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1480.11290211844042505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGC150.002223186769.978636
CACGATA150.002223186769.9786321
CGACAAT150.002223186769.9786332
AGACGGT150.002223186769.9786310
TTCGACA150.002223186769.9786328
ACGGTTT150.002223186769.9786312
CGGCATT252.3822991E-456.00427245
CTTCGCT200.00692580152.52405565
GATCATC200.00692580152.52405555
ACGGCCG200.00692580152.52405551
GCTAGAC200.00692580152.52405552
TCCGGAG200.00694675152.4839834
ACGCCGA200.00694675152.4839841
CGAGTCC200.00694675152.4839825
CCTAGAG200.00694675152.483983
ATCGTAC200.00694675152.4839810
AAGTTAG200.00694675152.4839830
CGAATTC200.00694675152.4839814
GACGGTT200.00694675152.4839811
TCGTACT200.00694675152.4839811