##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779793_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131087
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59932716440227	34.0	34.0	34.0	34.0	34.0
2	33.67816030575114	34.0	34.0	34.0	34.0	34.0
3	33.77126641085691	34.0	34.0	34.0	34.0	34.0
4	33.60681837253122	34.0	34.0	34.0	34.0	34.0
5	33.64308436381945	34.0	34.0	34.0	34.0	34.0
6	37.342505359036366	38.0	38.0	38.0	37.0	38.0
7	37.39336471198517	38.0	38.0	38.0	37.0	38.0
8	37.303699070083226	38.0	38.0	38.0	37.0	38.0
9	37.22289777018316	38.0	38.0	38.0	36.0	38.0
10-11	37.17275931251764	38.0	38.0	38.0	36.5	38.0
12-13	37.32781282659607	38.0	38.0	38.0	36.5	38.0
14-15	37.35289921960225	38.0	38.0	38.0	37.0	38.0
16-17	37.26236774050821	38.0	38.0	38.0	36.0	38.0
18-19	37.144800781160605	38.0	38.0	38.0	36.0	38.0
20-21	37.156300777346345	38.0	38.0	38.0	36.0	38.0
22-23	37.16461586579905	38.0	38.0	38.0	36.0	38.0
24-25	37.30133422841319	38.0	38.0	38.0	36.0	38.0
26-27	37.21836642840251	38.0	38.0	38.0	36.0	38.0
28-29	36.77099178408233	38.0	37.0	38.0	34.0	38.0
30-31	37.00498142455011	38.0	38.0	38.0	35.5	38.0
32-33	37.1151449037662	38.0	38.0	38.0	36.0	38.0
34-35	37.16402465538154	38.0	38.0	38.0	36.0	38.0
36-37	37.114668121171434	38.0	38.0	38.0	36.0	38.0
38-39	36.310934722741386	38.0	37.0	38.0	34.0	38.0
40-41	36.808402053597995	38.0	37.0	38.0	35.0	38.0
42-43	37.20151502437312	38.0	38.0	38.0	36.5	38.0
44-45	36.994537978594366	38.0	38.0	38.0	35.5	38.0
46-47	37.12024838466057	38.0	38.0	38.0	36.0	38.0
48-49	36.80022809279333	38.0	37.0	38.0	34.5	38.0
50-51	36.91541495342788	38.0	37.5	38.0	35.5	38.0
52-53	36.848489934165855	38.0	37.5	38.0	35.0	38.0
54-55	36.83860337028081	38.0	37.5	38.0	35.5	38.0
56-57	36.732292294430415	38.0	37.0	38.0	34.5	38.0
58-59	36.799682653504924	38.0	37.0	38.0	35.0	38.0
60-61	36.475973971484585	38.0	37.0	38.0	34.0	38.0
62-63	36.3464454903995	38.0	37.0	38.0	34.0	38.0
64-65	36.714060128006594	38.0	37.0	38.0	34.5	38.0
66-67	36.476969493542455	38.0	37.0	38.0	34.0	38.0
68-69	36.542677763622635	38.0	37.0	38.0	34.0	38.0
70-71	35.5013845766552	38.0	36.5	38.0	28.5	38.0
72-73	33.59859482633671	38.0	34.5	38.0	20.0	38.0
74-75	33.30225727951665	38.0	34.0	38.0	19.0	38.0
76	33.073851716798764	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	4.0
23	19.0
24	28.0
25	50.0
26	111.0
27	222.0
28	393.0
29	670.0
30	1155.0
31	1786.0
32	2780.0
33	4422.0
34	7730.0
35	16154.0
36	21895.0
37	73663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.95168602787403	15.935978262528813	11.302263811079394	37.81007189851776
2	17.502879766872383	15.81163654672088	42.99739867416296	23.688085012243775
3	16.542448907977146	18.076544584894002	40.324364734870734	25.05664177225812
4	11.823445498028027	27.844866386445645	33.24509676779543	27.0865913477309
5	12.095020864006347	30.433986589059174	44.20270507372966	13.268287473204818
6	25.52350728905231	45.124230472892044	16.77435596207099	12.577906275984652
7	24.281583986207632	26.35425328217138	18.821088284879508	30.543074446741475
8	21.85876555264824	43.87620435283438	15.805533729507884	18.4594963650095
9	20.69770457787576	15.96573268134903	16.758336066886876	46.57822667388834
10-11	21.34460320245333	28.942229206557478	28.962444788575525	20.750722802413666
12-13	21.10850046152555	19.355466217092467	30.297054627842577	29.2389786935394
14-15	19.1510218404571	19.646112886861395	20.60044092854364	40.60242434413786
16-17	27.743407050279583	29.26033855378489	20.355565387872176	22.640689008063347
18-19	28.430736838893257	23.402015455384593	21.213774058449733	26.953473647272425
20-21	19.723923806327097	21.510904971507472	21.18745565921869	37.57771556294674
22-23	19.515283742857797	30.1326599891675	21.023061020543608	29.328995247431095
24-25	27.90490103711728	29.833809489298886	20.892630326239946	21.368659147343887
26-27	28.00048822537704	30.86652375903026	21.35413885434864	19.778849161244057
28-29	27.13198105075255	22.99274527603805	22.010573130821516	27.864700542387883
30-31	28.426922578135134	21.571170291485807	30.12579431980288	19.87611281057618
32-33	20.892231876540006	30.254334907351605	29.70698848856103	19.146444727547355
34-35	26.578913240824797	30.3725769908534	23.878416624074088	19.170093144247712
36-37	20.875449129204267	29.408713297275856	21.660423993225873	28.055413580294005
38-39	18.691021993027533	23.362347143500116	30.02700496616751	27.919625897304844
40-41	18.78752279020803	20.86171779047503	31.581697651178224	28.769061768138716
42-43	27.2705427332334	20.341148783436893	30.667536321436607	21.720772161893102
44-45	19.081983720735085	28.71489926537338	32.10959134010237	20.093525673789163
46-47	26.81425312960095	20.793061096828826	25.145514047922372	27.24717172564785
48-49	25.817968219579363	20.07254723961949	23.670920838832227	30.438563701968924
50-51	18.987708764086094	20.35225720803546	32.17313018333854	28.486903844539903
52-53	17.285071671391343	20.361771696954204	40.36986024364839	21.98329638800606
54-55	16.41544426297071	20.7512249477203	33.5891349808435	29.244195808465495
56-57	24.867393743274288	20.635059949476062	25.3730147221565	29.12453158509315
58-59	17.200288464840487	21.018250361534967	39.07935454084105	22.702106632783494
60-61	24.997710622710624	28.589743589743588	25.17399267399267	21.238553113553113
62-63	16.973681198348682	38.95014765694751	22.09703388860485	21.97913725609896
64-65	16.803747730151223	40.191964353837	21.20916179634688	21.7951261196649
66-67	16.7418328334974	40.18757201834512	20.621093839426752	22.44950130873072
68-69	16.92263090033798	40.078429577411065	20.599208074889567	22.399731447361393
70-71	16.890077360880113	38.61025069807894	20.85539466255703	23.644277278483912
72-73	18.260375256567755	34.81195205139748	21.090441563594467	25.837231128440298
74-75	19.009071557729136	33.6799700920889	21.261701851696436	26.04925649848553
76	19.669629573112577	33.87708388967307	20.973562736047	25.47972380116736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	5.0
15	8.0
16	15.0
17	23.0
18	30.5
19	58.5
20	91.5
21	104.0
22	98.5
23	123.0
24	236.0
25	319.0
26	403.5
27	505.0
28	620.5
29	719.0
30	879.5
31	1093.0
32	1490.5
33	1835.0
34	1995.5
35	2338.0
36	2857.0
37	3194.0
38	3507.5
39	4338.0
40	7779.5
41	12132.5
42	13561.0
43	12716.5
44	10646.0
45	8523.5
46	7627.0
47	7208.0
48	6430.5
49	5831.0
50	5590.0
51	5267.0
52	4621.5
53	4047.5
54	3796.0
55	3615.0
56	3295.5
57	2839.5
58	2522.0
59	2201.5
60	1774.5
61	1528.5
62	1389.0
63	1329.0
64	1144.0
65	1019.0
66	958.0
67	897.0
68	829.0
69	692.5
70	583.0
71	542.0
72	565.0
73	531.5
74	437.0
75	399.0
76	369.5
77	317.5
78	275.0
79	255.0
80	218.5
81	180.0
82	157.5
83	137.0
84	102.0
85	70.0
86	61.0
87	49.0
88	38.5
89	23.5
90	14.5
91	7.0
92	4.0
93	4.5
94	4.0
95	1.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05263679846209006
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001907130379061234
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.8142607581224686E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.013731338729240886
52-53	0.028606955685918513
54-55	0.04653398124909411
56-57	0.045771129097469616
58-59	0.03699832935378795
60-61	0.0358540511263512
62-63	0.030514086064979743
64-65	0.01601989518411437
66-67	0.03356549467147772
68-69	0.010679930122742912
70-71	0.009917077971118418
72-73	0.022885564548734808
74-75	0.013731338729240886
76	0.01678274733573886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	131087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.301913996048423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.9026320585249	11.692234927948613
2	19.736533805420322	11.566364322930573
3	14.844705943609904	13.049348905688587
4	9.911223347478588	11.616712564937789
5	5.987868058629039	8.772799743681677
6	3.3453958501470935	5.881590089024846
7	1.8458254145947774	3.786035228512362
8	1.2001770326208638	2.8133987351911327
9	0.7159407461404285	1.888059075270622
>10	2.4654395876181305	11.034656373248302
>50	0.02082736716044883	0.43330002212271246
>100	0.015620525370336622	1.1458039317399895
>500	0.0	0.0
>1k	0.005206841790112208	2.7950902835521445
>5k	0.0	0.0
>10k+	0.002603420895056104	13.524605796150649
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	17729	13.524605796150649	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2394	1.8262680509890379	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1270	0.9688222325631068	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	427	0.3257378687436588	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	318	0.24258698421658897	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	269	0.2052072287869888	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	222	0.1693531776606376	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	148	0.11290211844042505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00839137366786943	0.0
2	0.0	0.0	0.0	0.00839137366786943	0.0
3	0.0	0.0	0.0	0.00839137366786943	0.0
4	0.0	0.0	0.0	0.009917077971118418	0.0
5	0.0	0.0	0.0	0.010679930122742912	0.0
6	0.0	0.0	0.0	0.010679930122742912	0.0
7	0.0	0.0	0.0	0.010679930122742912	0.0
8	0.0	0.0	0.0	0.010679930122742912	0.0
9	0.0	0.0	0.0	0.015257043032489873	0.0
10	0.0	0.0	0.0	0.021359860245485824	0.0
11	0.0	0.0	0.0	0.024411268851983796	0.0
12	0.0	0.0	0.0	0.028225529610106267	0.0
13	0.0	0.0	0.0	0.02975123391335525	0.0
14	0.0	0.0	0.0	0.02975123391335525	0.0
15	0.0	0.0	0.0	0.035091198974726706	0.0
16	0.0	0.0	0.0	0.035091198974726706	0.0
17	0.0	0.0	0.0	0.035091198974726706	0.0
18	0.0	0.0	0.0	0.038905459732849174	0.0
19	0.0	0.0	0.0	0.04271972049097165	0.0
20	0.0	0.0	0.0	0.045008276945845124	0.0
21	0.0	0.0	0.0	0.045008276945845124	0.0
22	0.0	0.0	0.0	0.045008276945845124	0.0
23	0.0	0.0	0.0	0.04729683340071861	0.0
24	0.0	0.0	0.0	0.04729683340071861	0.0
25	0.0	0.0	0.0	0.04882253770396759	0.0
26	0.0	0.0	0.0	0.051111094158841075	0.0
27	0.0	0.0	0.0	0.08772799743681677	0.0
28	0.0	0.0	0.0	0.1090878576823026	0.0
29	0.0	0.0	0.0	0.1327362743826619	0.0
30	0.0	0.0	0.0	0.14875616956677626	0.0
31	0.0	0.0	0.0	0.18766162929962543	0.0
32	0.0	0.0	0.0	0.2517412100360829	0.0
33	0.0	0.0	0.0	0.3142950864692914	0.0
34	0.0	0.0	0.0	0.3966831188447367	0.0
35	0.0	0.0	0.0	0.48517396843317795	0.0
36	0.0	0.0	0.0	0.7025868316461586	0.0
37	0.0	0.0	0.0	1.0496845606353034	0.0
38	0.0	0.0	0.0	1.4173792977183093	0.0
39	0.0	0.0	0.0	1.8407622418699032	0.0
40	0.0	0.0	0.0	2.4159527641947713	0.0
41	0.0	0.0	0.0	3.0361515634654848	0.0
42	0.0	0.0	0.0	3.793663750028607	0.0
43	0.0	0.0	0.0	4.6724694287000235	0.0
44	0.0	0.0	0.0	5.485669822331734	0.0
45	0.0	0.0	0.0	6.211142218526628	0.0
46	0.0	0.0	0.0	7.03502254228108	0.0
47	0.0	0.0	0.0	7.809317476179942	0.0
48	0.0	0.0	0.0	8.486730186822491	0.0
49	0.0	0.0	0.0	9.113794655457825	0.0
50	0.0	0.0	0.0	9.797310183313373	0.0
51	0.0	0.0	0.0	10.518968318750144	0.0
52	0.0	0.0	0.0	11.317674521500988	0.0
53	0.0	0.0	0.0	12.123246393616453	0.0
54	0.0	0.0	0.0	12.875418615118203	0.0
55	0.0	0.0	0.0	13.632930801681326	0.0
56	0.0	0.0	0.0	14.389680136092824	0.0
57	0.0	0.0	0.0	15.030475943457398	0.0
58	0.0	0.0	0.0	15.602615057175768	0.0
59	0.0	0.0	0.0	16.05422353093747	0.0
60	0.0	0.0	0.0	16.618734123139593	0.0
61	0.0	0.0	0.0	17.09704242220815	0.0
62	0.0	0.0	0.0	17.664604423016776	0.0
63	0.0	0.0	0.0	18.353459915933694	0.0
64	0.0	0.0	0.0	18.976710123810903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGC	15	0.0022231867	69.97863	6
CACGATA	15	0.0022231867	69.97863	21
CGACAAT	15	0.0022231867	69.97863	32
AGACGGT	15	0.0022231867	69.97863	10
TTCGACA	15	0.0022231867	69.97863	28
ACGGTTT	15	0.0022231867	69.97863	12
CGGCATT	25	2.3822991E-4	56.004272	45
CTTCGCT	20	0.006925801	52.524055	65
GATCATC	20	0.006925801	52.524055	55
ACGGCCG	20	0.006925801	52.524055	51
GCTAGAC	20	0.006925801	52.524055	52
TCCGGAG	20	0.006946751	52.48398	34
ACGCCGA	20	0.006946751	52.48398	41
CGAGTCC	20	0.006946751	52.48398	25
CCTAGAG	20	0.006946751	52.48398	3
ATCGTAC	20	0.006946751	52.48398	10
AAGTTAG	20	0.006946751	52.48398	30
CGAATTC	20	0.006946751	52.48398	14
GACGGTT	20	0.006946751	52.48398	11
TCGTACT	20	0.006946751	52.48398	11
>>END_MODULE