##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779792_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65131
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.859083999938587	23.0	12.0	33.0	12.0	34.0
2	23.19864580614454	23.0	12.0	34.0	12.0	34.0
3	23.0780273602432	23.0	11.0	34.0	11.0	34.0
4	23.762693648185962	23.0	12.0	34.0	12.0	34.0
5	22.802275414165297	23.0	11.0	34.0	11.0	34.0
6	24.64734151172253	26.0	12.0	37.0	11.0	38.0
7	24.956840828484133	26.0	12.0	37.0	11.0	38.0
8	25.198599745129048	26.0	11.0	37.0	11.0	38.0
9	25.454791113294746	27.0	12.0	37.0	11.0	38.0
10-11	24.550122061691052	26.0	11.5	36.0	11.0	38.0
12-13	24.619067725046445	26.0	11.0	37.0	11.0	38.0
14-15	23.967196880133883	25.5	11.0	35.0	11.0	38.0
16-17	24.05268612488677	24.5	11.0	36.0	11.0	38.0
18-19	24.001404860972503	25.0	11.0	37.0	11.0	38.0
20-21	23.968294667669774	24.5	11.0	35.0	11.0	38.0
22-23	24.182486066542815	24.5	11.0	36.5	11.0	38.0
24-25	24.421895871397645	26.0	11.0	37.0	11.0	38.0
26-27	23.70447252460426	23.0	11.0	36.0	10.5	38.0
28-29	23.890382459965302	23.5	11.0	36.5	11.0	38.0
30-31	23.948181357571663	24.0	11.0	37.0	11.0	38.0
32-33	24.182777786307597	26.0	11.0	37.0	11.0	38.0
34-35	24.250825259860896	26.0	11.0	37.0	11.0	38.0
36-37	24.026538821759225	25.5	11.0	37.0	11.0	38.0
38-39	23.893698853080714	24.5	11.0	37.0	11.0	38.0
40-41	23.825344306090802	24.5	11.0	36.5	11.0	38.0
42-43	24.02137231118822	25.5	11.0	37.0	11.0	38.0
44-45	24.169757872595234	25.5	11.0	37.0	11.0	38.0
46-47	24.081735271990297	25.0	11.0	37.0	11.0	38.0
48-49	23.722528442677067	24.0	11.0	36.5	11.0	38.0
50-51	23.477445456080822	23.0	11.0	36.5	11.0	38.0
52-53	23.872679676344596	24.5	11.0	36.5	11.0	38.0
54-55	23.847530361886044	24.0	11.0	37.0	11.0	38.0
56-57	23.864181418986348	23.5	11.0	37.0	11.0	38.0
58-59	23.714644332192044	23.5	11.0	37.0	11.0	38.0
60-61	23.95252644669973	24.0	11.0	37.0	11.0	38.0
62-63	23.708648723342186	23.5	11.0	37.0	11.0	38.0
64-65	23.67005726919593	23.5	11.0	37.0	11.0	38.0
66-67	23.975004222259756	24.0	11.0	37.0	11.0	38.0
68-69	23.770301392578034	24.0	11.0	36.0	11.0	38.0
70-71	23.945448403985814	24.5	11.0	36.0	11.0	38.0
72-73	23.71718536488001	23.5	11.0	36.0	11.0	38.0
74-75	23.571425281356035	23.5	11.0	36.5	11.0	38.0
76	23.155363805254026	23.0	11.0	35.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	67.0
13	794.0
14	2590.0
15	4160.0
16	5387.0
17	6250.0
18	6484.0
19	5633.0
20	4286.0
21	3101.0
22	2099.0
23	1441.0
24	1061.0
25	776.0
26	686.0
27	576.0
28	555.0
29	561.0
30	586.0
31	647.0
32	742.0
33	1008.0
34	1423.0
35	2050.0
36	3740.0
37	8426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.44505688535413	21.854416483702078	16.632632694108796	38.067893936835
2	12.99381247025226	24.04538545393131	37.49213124318681	25.468670832629627
3	11.5321429119774	21.567302820469518	33.90090740200519	32.999646865547895
4	9.807925565398966	40.77167554620688	20.454161612749687	28.966237275644467
5	9.553054613010701	41.878675285194454	27.963642505105096	20.60462759668975
6	23.12641066191712	42.65381013066376	12.318629182084786	21.90115002533434
7	16.66692256905526	29.845383776812174	20.29817746318844	33.189516190944126
8	13.578995854444956	49.72823583602027	12.795946568401659	23.896821741133117
9	18.34722925975402	23.785833832358318	13.90360372810048	43.963333179787185
10-11	15.580377706126209	34.9355135882082	21.034853370182713	28.44925533548288
12-13	15.164053983510156	26.05594878015077	24.815372096236814	33.96462514010226
14-15	25.938493190646543	29.347008337043807	17.824077628164776	26.89042084414488
16-17	26.97891156984823	26.348638502698428	16.850016505323925	29.822433422129418
18-19	18.064639950867495	26.44633809304468	22.330723169046525	33.1582987870413
20-21	25.488630606009426	25.001151525387293	18.438992184981036	31.071225683622238
22-23	27.234761246737293	24.8533701827115	16.899278366344234	31.01259020420697
24-25	17.763839734682442	33.16930116153202	16.649905188892898	32.41695391489264
26-27	24.24670469288582	32.64292458986189	17.884094241561176	25.22627647569111
28-29	18.861174447438525	31.284307177349397	21.574272399947795	28.280245975264286
30-31	21.049884079777677	29.23339116549723	21.416836836529455	28.299887918195637
32-33	17.966099092597997	31.742181142620257	21.358492883573106	28.93322688120864
34-35	16.82110257256696	35.196800221097966	20.113773328522406	27.868323877812667
36-37	23.164804790970862	26.40945909631848	21.927137318131216	28.498598794579443
38-39	24.09350888641511	23.669724770642205	24.9502898161299	27.28647652681279
40-41	19.132978151724984	30.364189095822265	21.89126529609556	28.61156745635719
42-43	14.280450169658074	29.608788441755845	24.370883296740413	31.739878091845664
44-45	14.413480730846	25.270228773222787	27.954859511745738	32.361430984185475
46-47	17.391504617723154	28.755786548338307	23.42333351246363	30.429375321474904
48-49	18.798268105817506	29.018439759868574	22.30044065038154	29.88285148393238
50-51	25.944066144126023	27.900138951796777	17.894074204865614	28.261720699211583
52-53	13.240081071121484	32.244963763665396	22.081132539000123	32.433822626212994
54-55	16.33398845350694	27.40526348114482	21.348728657413094	34.91201940793514
56-57	18.50796916746	29.753094002395358	19.10604059822498	32.63289623191966
58-59	16.98707121579707	29.88821668765163	20.557841722200042	32.56687037435126
60-61	14.282094789131419	27.640559859344172	21.705682280589954	36.37166307093446
62-63	16.758794355643587	28.97569364472492	20.391696224300212	33.87381577533128
64-65	17.259011862259587	27.51122883795923	20.43686897769588	34.7928903220853
66-67	14.595569887519675	35.95761833467696	16.673960612691467	32.7728511651119
68-69	15.641553177442386	39.00523560209424	15.117992968018301	30.235218252445073
70-71	14.204449494096513	39.96944619305707	16.74548218206384	29.080622130782572
72-73	15.842161830185782	39.49792722247812	16.688162137263934	27.97174881007216
74-75	17.308755335810584	38.63050087522649	15.655037926480976	28.405705862481955
76	15.542760632580993	42.08659603869185	14.076462459695993	28.294180869031166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	1.0
15	8.0
16	12.5
17	10.0
18	11.0
19	15.5
20	35.0
21	51.0
22	57.0
23	67.0
24	102.5
25	134.0
26	149.5
27	180.0
28	263.0
29	331.0
30	364.0
31	501.5
32	692.5
33	779.0
34	867.0
35	1085.0
36	1389.0
37	1563.0
38	1739.5
39	2087.0
40	2489.5
41	2902.0
42	3083.0
43	3251.5
44	3584.5
45	3875.0
46	4001.0
47	4010.5
48	3964.0
49	3811.5
50	3715.0
51	3663.0
52	3440.5
53	3097.5
54	2925.0
55	2764.5
56	2463.5
57	2151.0
58	1979.0
59	1815.5
60	1547.0
61	1283.0
62	1124.0
63	1054.0
64	878.0
65	682.0
66	535.0
67	478.0
68	444.0
69	389.5
70	324.0
71	279.0
72	234.5
73	176.5
74	141.0
75	119.0
76	112.5
77	87.5
78	69.5
79	70.0
80	54.0
81	34.0
82	31.5
83	33.0
84	29.5
85	16.5
86	13.5
87	20.0
88	15.0
89	10.5
90	10.5
91	8.0
92	6.0
93	7.0
94	6.0
95	4.5
96	5.0
97	5.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003070734366123658
7	0.003070734366123658
8	0.001535367183061829
9	0.006141468732247316
10-11	0.001535367183061829
12-13	0.0
14-15	0.0
16-17	7.676835915309145E-4
18-19	0.001535367183061829
20-21	0.0
22-23	0.001535367183061829
24-25	0.0023030507745927437
26-27	7.676835915309145E-4
28-29	0.003838417957654573
30-31	0.0
32-33	0.0
34-35	0.0023030507745927437
36-37	0.01305062105602555
38-39	0.005373785140716402
40-41	0.0
42-43	0.0
44-45	0.001535367183061829
46-47	0.0023030507745927437
48-49	0.0
50-51	7.676835915309145E-4
52-53	0.0046061015491854875
54-55	0.0046061015491854875
56-57	0.007676835915309146
58-59	0.007676835915309146
60-61	0.01151525387296372
62-63	0.006141468732247316
64-65	0.01305062105602555
66-67	0.01305062105602555
68-69	0.0
70-71	0.0
72-73	0.001535367183061829
74-75	0.007676835915309146
76	0.001535367183061829
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	65131.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.73088084015292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.00770038501925	82.47378360534921
2	2.4361218060903043	4.274462237644133
3	1.536576828841442	4.044157160184858
4	0.9327966398319916	3.2734028342878196
5	0.4497724886244312	1.9729468302344506
6	0.33776688834441726	1.7779551979855983
7	0.1662583129156458	1.0210191767361165
8	0.05775288764438222	0.4053369363283229
9	0.028001400070003502	0.2210928743609034
>10	0.04725236261813091	0.5358431468885784
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009212203098370975	0.0
2	0.0	0.0	0.0	0.013818304647556463	0.0
3	0.0	0.0	0.0	0.013818304647556463	0.0
4	0.0	0.0	0.0	0.01688903901368012	0.0
5	0.0	0.0	0.0	0.03224271084429842	0.0
6	0.0	0.0	0.0	0.03224271084429842	0.0
7	0.0	0.0	0.0	0.03224271084429842	0.0
8	0.0	0.0	0.0	0.03224271084429842	0.0
9	0.0	0.0	0.0	0.03224271084429842	0.0
10	0.0	0.0	0.0	0.03377807802736024	0.0
11	0.0	0.0	0.0	0.03377807802736024	0.0
12	0.0	0.0	0.0	0.03377807802736024	0.0
13	0.0	0.0	0.0	0.03377807802736024	0.0
14	0.0	0.0	0.0	0.03377807802736024	0.0
15	0.0	0.0	0.0	0.03377807802736024	0.0
16	0.0	0.0	0.0	0.03377807802736024	0.0
17	0.0	0.0	0.0	0.03377807802736024	0.0
18	0.0	0.0	0.0	0.042990281125731215	0.0
19	0.0	0.0	0.0	0.047596382674916705	0.0
20	0.0	0.0	0.0	0.052202484224102196	0.0
21	0.0	0.0	0.0	0.06602078887165866	0.0
22	0.0	0.0	0.0	0.09672813253289524	0.0
23	0.0	0.0	0.0	0.09672813253289524	0.0
24	0.0	0.0	0.0	0.1105464371804517	0.0
25	0.0	0.0	0.0	0.11975864027882269	0.0
26	0.0	0.0	0.0	0.12129400746188451	0.0
27	0.0	0.0	0.0	0.12590010901107	0.0
28	0.0	0.0	0.0	0.13818304647556462	0.0
29	0.0	0.0	0.0	0.14278914802475012	0.0
30	0.0	0.0	0.0	0.17042575731986304	0.0
31	0.0	0.0	0.0	0.18731479633354317	0.0
32	0.0	0.0	0.0	0.20420383534722328	0.0
33	0.0	0.0	0.0	0.22723434309315071	0.0
34	0.0	0.0	0.0	0.2932551319648094	0.0
35	0.0	0.0	0.0	0.36541738956871533	0.0
36	0.0	0.0	0.0	0.5511968187191967	0.0
37	0.0	0.0	0.0	0.7692189587139765	0.0
38	0.0	0.0	0.0	0.997988668990189	0.0
39	0.0	0.0	0.0	1.2789608634905039	0.0
40	0.0	0.0	0.0	1.5184781440481492	0.0
41	0.0	0.0	0.0	1.7595307917888563	0.0
42	0.0	0.0	0.0	2.1372311188220663	0.0
43	0.0	0.0	0.0	2.4980424068415963	0.0
44	0.0	0.0	0.0	2.849641491762755	0.0
45	0.0	0.0	0.0	3.141361256544503	0.0
46	0.0	0.0	0.0	3.5390213569575164	0.0
47	0.0	0.0	0.0	3.8353472232884496	0.0
48	0.0	0.0	0.0	4.1854109410265465	0.0
49	0.0	0.0	0.0	4.475595338625232	0.0
50	0.0	0.0	0.0	4.738143126928805	0.0
51	0.0	0.0	0.0	5.200288649030416	0.0
52	0.0	0.0	0.0	5.381461976631711	0.0
53	0.0	0.0	0.0	5.599484116626491	0.0
54	0.0	0.0	0.0	5.975649076476639	0.0
55	0.0	0.0	0.0	6.205954153935914	0.0
56	0.0	0.0	0.0	6.4746434109717335	0.0
57	0.0	0.0	0.0	6.651210637023844	0.0
58	0.0	0.0	0.0	6.913758425327417	0.0
59	0.0	0.0	0.0	7.208548924475288	0.0
60	0.0	0.0	0.0	7.388186884893522	0.0
61	0.0	0.0	0.0	7.5785724155931895	0.0
62	0.0	0.0	0.0	7.753604274462238	0.0
63	0.0	0.0	0.0	7.994656922202945	0.0
64	0.0	0.0	0.0	8.21728516374691	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGA	20	7.872656E-5	70.00001	36
CTATCGG	15	0.0022154627	70.0	42
TTTGTTA	15	0.0022154627	70.0	9
TGGCCAC	15	0.0022154627	70.0	49
GGCTTAG	25	2.8109826E-6	70.0	29
TCGTGTA	15	0.0022154627	70.0	34
GGTGGAT	15	0.0022154627	70.0	47
CTAGGTG	15	0.0022154627	70.0	44
TATCGGT	15	0.0022154627	70.0	43
TGGTCGC	100	0.0	66.5	49
GGTGGTC	110	0.0	63.63636	47
CGGTGGT	105	0.0	63.333332	46
TTACGCT	35	2.9322473E-7	60.0	13
GTGGTCG	105	0.0	59.999996	48
GGTCGCC	135	0.0	59.629627	50
TTAGGTG	30	8.292001E-6	58.333332	32
GCCCACG	30	8.292001E-6	58.333332	20
CCGAATC	30	8.292001E-6	58.333332	55
CCGGTGG	30	8.292001E-6	58.333332	45
CTCGGTG	100	0.0	55.999996	44
>>END_MODULE