FastQCFastQC Report
Wed 25 May 2016
SRR1779792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65131
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3060146.98377116887504No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG40906.279651778722882No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC24663.786215473430471No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT7301.1208180436351354TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4870.7477238181511108No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT3510.5389138812547021No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3380.5189541078748983No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2760.4237613425250648TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1900.29171976478174755RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1880.2886490304156239No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1530.23491117900845984No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1430.21955750717784156No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT1280.19652699943191412No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT1200.18424406196741952No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG1060.1627489214045539No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG990.1520013511231211No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT950.14585988239087377No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC920.1412537808416883Illumina PCR Primer Index 8 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC850.13050621056025546TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGACATCTCGTATGCCGT810.12436474182800816No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCG760.11668790591269902No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT740.11361717154657536RNA PCR Primer, Index 12 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC710.10901106999738988No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGAG150.002205242670.08153551
CGTCTTA207.827308E-570.08153548
CGCTGCC150.002218743869.97388525
TGCCGCA150.002218743869.97388528
GCGGTAG150.002218743869.97388518
AGAGACT207.88722E-569.97388523
AGACCAC150.002218743869.97388517
GCTTGAC501.2732926E-1163.02490656
TTAATAC401.049375E-861.2271462
TTGAACA308.272531E-658.3563958
TTGACAA553.45608E-1157.29537258
ACAACCA252.369617E-456.0221470
GTATGCA252.3786434E-455.9791142
TGCAGTC252.3786434E-455.9791145
CTTAATA452.6426278E-854.4659651
TAATACA452.6588168E-854.424133
AGGTGCC200.00689091852.5611553
TGCTTGA43900.052.5606355
GGACTGT200.006911927452.52075247
GACAAAC200.006911927452.52075260
Produced by FastQC (version 0.11.5)