##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779792_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65131
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.62755062873286	34.0	34.0	34.0	34.0	34.0
2	33.69309545377777	34.0	34.0	34.0	34.0	34.0
3	33.76327708771553	34.0	34.0	34.0	34.0	34.0
4	33.56240499915555	34.0	34.0	34.0	34.0	34.0
5	33.646051803288756	34.0	34.0	34.0	34.0	34.0
6	37.38571494372879	38.0	38.0	38.0	37.0	38.0
7	37.43324991171639	38.0	38.0	38.0	37.0	38.0
8	37.31838909275153	38.0	38.0	38.0	37.0	38.0
9	37.27516850654834	38.0	38.0	38.0	37.0	38.0
10-11	37.192105141944694	38.0	38.0	38.0	36.5	38.0
12-13	37.361671093642045	38.0	38.0	38.0	36.5	38.0
14-15	37.374268781379065	38.0	38.0	38.0	37.0	38.0
16-17	37.129638728101824	38.0	38.0	38.0	36.0	38.0
18-19	36.922817091707486	38.0	38.0	38.0	35.0	38.0
20-21	37.0701048655786	38.0	38.0	38.0	35.5	38.0
22-23	37.186892570358204	38.0	38.0	38.0	36.0	38.0
24-25	37.21032227357172	38.0	38.0	38.0	36.0	38.0
26-27	37.12610738358078	38.0	38.0	38.0	35.5	38.0
28-29	36.54494019744822	38.0	37.0	38.0	34.0	38.0
30-31	36.617041040364796	38.0	37.0	38.0	34.0	38.0
32-33	36.68240929818366	38.0	37.0	38.0	35.0	38.0
34-35	36.43448588229875	38.0	37.0	38.0	34.0	38.0
36-37	36.454814143802494	38.0	37.0	38.0	34.5	38.0
38-39	34.398212832598915	37.0	34.5	38.0	25.5	38.0
40-41	36.11063088237552	38.0	36.5	38.0	32.5	38.0
42-43	37.06364096973792	38.0	37.5	38.0	35.5	38.0
44-45	36.855621746940784	38.0	37.5	38.0	35.0	38.0
46-47	36.98536027390951	38.0	38.0	38.0	35.5	38.0
48-49	36.55789869647326	38.0	37.0	38.0	34.0	38.0
50-51	36.57096467120112	38.0	37.0	38.0	34.0	38.0
52-53	36.498579785355666	38.0	37.0	38.0	34.0	38.0
54-55	36.547557998495336	38.0	37.0	38.0	34.0	38.0
56-57	36.38378038107814	38.0	37.0	38.0	34.0	38.0
58-59	36.322726505043676	38.0	37.0	38.0	34.0	38.0
60-61	35.86679154319755	38.0	37.0	38.0	31.5	38.0
62-63	35.83914725706653	38.0	37.0	38.0	32.5	38.0
64-65	36.39617079424545	38.0	37.0	38.0	34.0	38.0
66-67	35.900584974896745	38.0	36.5	38.0	32.5	38.0
68-69	35.669389384471295	38.0	37.0	38.0	31.0	38.0
70-71	31.84883542399165	35.5	29.0	38.0	17.5	38.0
72-73	25.14229783052617	24.0	16.0	37.5	10.0	38.0
74-75	24.09238304340483	23.0	11.0	37.0	11.0	38.0
76	24.31318419800095	24.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	10.0
24	13.0
25	44.0
26	103.0
27	205.0
28	389.0
29	653.0
30	1040.0
31	1603.0
32	2547.0
33	4028.0
34	7212.0
35	17052.0
36	16090.0
37	14140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.019233132604155	7.765454098562123	12.490782843799925	66.72452992503379
2	6.4961385515345995	6.979779214199076	69.8238933841028	16.700188850163517
3	6.720302160261626	9.465538683576177	70.5762233038031	13.237935852359092
4	5.051358032273418	58.17199183184658	24.67642136616972	12.100228769710277
5	4.9070335170656065	20.963903517526216	68.54646788779537	5.582595077612811
6	9.88776465891818	69.14372572200642	13.786061936712166	7.182447682363237
7	8.883634521195743	19.87532818473538	12.384271698576715	58.85676559549216
8	7.859544610093503	68.70153997328461	8.206537593465477	15.232377823156407
9	7.501804056440098	14.604412645284121	7.018163393775621	70.87561990450017
10-11	8.413044479587294	40.79931215550199	35.65967051020251	15.127972854708204
12-13	8.432236569375567	10.346071763062136	41.626875067172314	39.59481660038998
14-15	8.836038138520827	8.398458491348206	14.018670064946031	68.74683330518494
16-17	38.29512827992814	35.28120249957777	7.787382352489598	18.636286868004483
18-19	40.30876234051373	15.757473399763553	8.275629116703259	35.65813514301945
20-21	10.851207566289478	9.173051235202898	8.123627765580139	71.8521134329275
22-23	8.786138705071318	39.425158526661654	8.130536917903916	43.65816585036311
24-25	38.339948257728715	38.40980800085981	8.109104029602108	15.141139711809368
26-27	40.27728731326097	42.44906419370192	8.818381415915615	8.455267077121494
28-29	10.329950407639988	15.7636148684958	35.50920452626246	38.39723019760176
30-31	8.028435000230305	38.552302283091	16.5359045615759	36.88335815510279
32-33	8.216517480155378	12.595384686247716	64.77099998464632	14.417097848950577
34-35	37.69403202775944	35.72031751393346	18.597135004836407	7.988515453470697
36-37	10.227848489966375	71.44370576223304	9.926916522086257	8.401529225714329
38-39	8.079869800862877	18.20791942393023	38.40490703351706	35.30730374168983
40-41	8.177365616987302	8.95502909520812	41.97617110131888	40.8914341864857
42-43	37.88211450768451	8.489812838740384	38.78798114569099	14.84009150788411
44-45	10.5909628287605	37.80764919930601	42.449831877293455	9.151556094640032
46-47	37.61188988346563	10.674640340237367	16.771583424175894	34.94188635212111
48-49	36.054259876249404	7.907908676359951	10.71916598854616	45.31866545884448
50-51	13.281022065936765	7.918861231822858	40.650768545675106	38.149348156565274
52-53	7.401747730867876	7.845591510143904	69.65966857616759	15.092992182820636
54-55	7.023755577914148	7.936190966275221	45.734671776714464	39.30538167909617
56-57	37.04050753467795	8.017788291678828	17.13928017327455	37.80242400036867
58-59	9.67593303639994	9.410996774688988	65.89847949623714	15.014590692673938
60-61	36.43714437098298	36.32503244334892	18.20590200188901	9.031921183779094
62-63	8.81210166628273	73.01850572064808	8.620901481993396	9.548491131075789
64-65	6.503078789367811	76.21884741182072	8.26973573084778	9.008338067963699
66-67	6.816558778133566	75.95813464181775	8.149615671097392	9.075690908951293
68-69	6.276198811552812	75.86101002656348	8.069617823636895	9.793173338246811
70-71	7.126899188502376	70.72082792718786	8.716881760880748	13.435391123429019
72-73	9.117782555282556	57.510749385749385	11.49416461916462	21.877303439803438
74-75	10.480453275189634	51.95313699597703	13.298836102324724	24.267573626508614
76	10.042072290636613	53.924699812670816	12.606332340386327	23.42689555630624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	9.0
17	15.0
18	17.0
19	23.5
20	32.0
21	36.0
22	54.0
23	75.5
24	97.0
25	115.0
26	137.5
27	191.5
28	210.0
29	197.0
30	250.0
31	331.0
32	386.0
33	413.0
34	446.5
35	508.0
36	779.0
37	1022.0
38	5601.0
39	12367.0
40	13116.0
41	9309.0
42	6940.0
43	5316.0
44	2850.0
45	1654.0
46	1300.0
47	1248.5
48	1044.0
49	870.5
50	850.0
51	802.0
52	754.5
53	737.5
54	720.0
55	702.0
56	673.5
57	621.5
58	580.0
59	543.0
60	498.0
61	461.0
62	432.0
63	403.0
64	362.0
65	288.0
66	240.5
67	255.0
68	233.0
69	176.0
70	135.5
71	130.0
72	112.0
73	88.5
74	95.0
75	107.0
76	88.0
77	52.0
78	33.5
79	32.0
80	29.0
81	23.5
82	15.5
83	10.0
84	11.0
85	12.0
86	7.5
87	3.0
88	3.5
89	2.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05373785140716402
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.676835915309145E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.012282937464494633
52-53	0.027636609295112927
54-55	0.04682869908338579
56-57	0.04913174985797853
58-59	0.03224271084429842
60-61	0.02686892570358201
62-63	0.024565874928989265
64-65	0.012282937464494633
66-67	0.02686892570358201
68-69	0.006141468732247316
70-71	0.006909152323778232
72-73	0.01688903901368012
74-75	0.007676835915309146
76	0.007676835915309146
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	65131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.575716632632695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.35534946844605	6.7003423868818235
2	13.232300384528386	3.5927592083646807
3	10.49536304003619	4.274462237644133
4	8.40307622709794	4.563111268059757
5	6.627459850712508	4.4986258463711595
6	4.331599185704592	3.528273786676084
7	2.985749830355123	2.8373585542982602
8	1.62859081655734	1.7687429948872273
9	0.7464374575887808	0.9120081067387267
>10	1.9000226193168965	4.044157160184858
>50	0.12440624293146346	1.3019913712364313
>100	0.12440624293146346	3.8077106139933363
>500	0.011309658448314861	1.1208180436351354
>1k	0.022619316896629722	10.065867252153353
>5k	0.0	0.0
>10k+	0.011309658448314861	46.98377116887504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	30601	46.98377116887504	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	4090	6.279651778722882	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	2466	3.786215473430471	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	730	1.1208180436351354	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	487	0.7477238181511108	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	351	0.5389138812547021	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	338	0.5189541078748983	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	276	0.4237613425250648	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	190	0.29171976478174755	RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT	188	0.2886490304156239	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	153	0.23491117900845984	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	143	0.21955750717784156	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	128	0.19652699943191412	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	120	0.18424406196741952	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	106	0.1627489214045539	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	99	0.1520013511231211	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT	95	0.14585988239087377	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC	92	0.1412537808416883	Illumina PCR Primer Index 8 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	85	0.13050621056025546	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGACATCTCGTATGCCGT	81	0.12436474182800816	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCG	76	0.11668790591269902	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	74	0.11361717154657536	RNA PCR Primer, Index 12 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	71	0.10901106999738988	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012282937464494634	0.0
2	0.0	0.0	0.0	0.01995977337980378	0.0
3	0.0	0.0	0.0	0.01995977337980378	0.0
4	0.0	0.0	0.0	0.021495140562865608	0.0
5	0.0	0.0	0.0	0.03991954675960756	0.0
6	0.0	0.0	0.0	0.03991954675960756	0.0
7	0.0	0.0	0.0	0.03991954675960756	0.0
8	0.0	0.0	0.0	0.03991954675960756	0.0
9	0.0	0.0	0.0	0.03991954675960756	0.0
10	0.0	0.0	0.0	0.047596382674916705	0.0
11	0.0	0.0	0.0	0.047596382674916705	0.0
12	0.0	0.0	0.0	0.047596382674916705	0.0
13	0.0	0.0	0.0	0.047596382674916705	0.0
14	0.0	0.0	0.0	0.047596382674916705	0.0
15	0.0	0.0	0.0	0.047596382674916705	0.0
16	0.0	0.0	0.0	0.047596382674916705	0.0
17	0.0	0.0	0.0	0.04913174985797854	0.0
18	0.0	0.0	0.0	0.05680858577328768	0.0
19	0.0	0.0	0.0	0.06448542168859683	0.0
20	0.0	0.0	0.0	0.07216225760390597	0.0
21	0.0	0.0	0.0	0.07983909351921512	0.0
22	0.0	0.0	0.0	0.07983909351921512	0.0
23	0.0	0.0	0.0	0.09058666380064792	0.0
24	0.0	0.0	0.0	0.0997988668990189	0.0
25	0.0	0.0	0.0	0.10747570281432804	0.0
26	0.0	0.0	0.0	0.10901106999738988	0.0
27	0.0	0.0	0.0	0.11668790591269902	0.0
28	0.0	0.0	0.0	0.12897084337719367	0.0
29	0.0	0.0	0.0	0.13511231210944097	0.0
30	0.0	0.0	0.0	0.16581965577067756	0.0
31	0.0	0.0	0.0	0.18577942915048135	0.0
32	0.0	0.0	0.0	0.20266846816416145	0.0
33	0.0	0.0	0.0	0.22416360872702706	0.0
34	0.0	0.0	0.0	0.28557829604950025	0.0
35	0.0	0.0	0.0	0.3608112880195299	0.0
36	0.0	0.0	0.0	0.5711565920990005	0.0
37	0.0	0.0	0.0	0.8106738726566458	0.0
38	0.0	0.0	0.0	1.034837481383673	0.0
39	0.0	0.0	0.0	1.320415777433173	0.0
40	0.0	0.0	0.0	1.5752867298214368	0.0
41	0.0	0.0	0.0	1.8224808462943913	0.0
42	0.0	0.0	0.0	2.1971104389614777	0.0
43	0.0	0.0	0.0	2.5840229690930587	0.0
44	0.0	0.0	0.0	2.9417635227464647	0.0
45	0.0	0.0	0.0	3.236554021894336	0.0
46	0.0	0.0	0.0	3.6388202238565355	0.0
47	0.0	0.0	0.0	3.9243985199060356	0.0
48	0.0	0.0	0.0	4.277532972010256	0.0
49	0.0	0.0	0.0	4.584606408622622	0.0
50	0.0	0.0	0.0	4.85176029847538	0.0
51	0.0	0.0	0.0	5.326188758041486	0.0
52	0.0	0.0	0.0	5.496614515361348	0.0
53	0.0	0.0	0.0	5.703889085074696	0.0
54	0.0	0.0	0.0	6.050882068446669	0.0
55	0.0	0.0	0.0	6.275045677173696	0.0
56	0.0	0.0	0.0	6.545270301392578	0.0
57	0.0	0.0	0.0	6.703413121247946	0.0
58	0.0	0.0	0.0	6.9460011361717156	0.0
59	0.0	0.0	0.0	7.223902596305907	0.0
60	0.0	0.0	0.0	7.391257619259646	0.0
61	0.0	0.0	0.0	7.573966314044004	0.0
62	0.0	0.0	0.0	7.741321336997743	0.0
63	0.0	0.0	0.0	7.98237398473845	0.0
64	0.0	0.0	0.0	8.212679062197724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCAGAG	15	0.0022052426	70.081535	51
CGTCTTA	20	7.827308E-5	70.081535	48
CGCTGCC	15	0.0022187438	69.973885	25
TGCCGCA	15	0.0022187438	69.973885	28
GCGGTAG	15	0.0022187438	69.973885	18
AGAGACT	20	7.88722E-5	69.973885	23
AGACCAC	15	0.0022187438	69.973885	17
GCTTGAC	50	1.2732926E-11	63.024906	56
TTAATAC	40	1.049375E-8	61.227146	2
TTGAACA	30	8.272531E-6	58.35639	58
TTGACAA	55	3.45608E-11	57.295372	58
ACAACCA	25	2.369617E-4	56.02214	70
GTATGCA	25	2.3786434E-4	55.97911	42
TGCAGTC	25	2.3786434E-4	55.97911	45
CTTAATA	45	2.6426278E-8	54.465965	1
TAATACA	45	2.6588168E-8	54.42413	3
AGGTGCC	20	0.006890918	52.56115	53
TGCTTGA	4390	0.0	52.56063	55
GGACTGT	20	0.0069119274	52.520752	47
GACAAAC	20	0.0069119274	52.520752	60
>>END_MODULE