##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779791_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56426
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.50680537340942	23.0	12.0	32.0	12.0	34.0
2	21.43311593946053	21.0	12.0	32.0	12.0	34.0
3	21.501559564739658	23.0	11.0	32.0	11.0	34.0
4	22.27620245985893	23.0	12.0	32.0	12.0	34.0
5	21.508701662354234	23.0	11.0	32.0	11.0	34.0
6	22.80225427994187	22.0	12.0	34.0	11.0	38.0
7	22.851770460426046	21.0	12.0	34.0	11.0	38.0
8	22.32717187112324	21.0	11.0	34.0	11.0	38.0
9	23.647166199978734	26.0	11.0	34.0	11.0	38.0
10-11	22.856085846949988	22.5	11.5	34.0	11.0	38.0
12-13	23.039724595044838	23.0	11.0	34.0	11.0	38.0
14-15	22.356989685605924	22.0	11.0	34.0	11.0	38.0
16-17	22.353055329103604	22.0	11.0	33.5	11.0	38.0
18-19	22.166616453408004	21.0	11.0	34.0	10.5	38.0
20-21	22.438556693722752	22.5	11.0	32.5	10.5	38.0
22-23	22.45160032609081	22.0	11.0	34.0	10.5	38.0
24-25	22.595753730549745	22.5	11.0	34.0	10.0	38.0
26-27	21.921206535994045	21.0	11.0	33.0	10.0	38.0
28-29	21.868677559990076	21.0	11.0	34.0	10.5	38.0
30-31	21.94545067876511	21.0	11.0	34.0	11.0	38.0
32-33	22.14262042320916	21.0	11.0	34.0	11.0	38.0
34-35	22.335767199517953	21.0	11.0	34.0	11.0	38.0
36-37	22.1295767908411	21.0	11.0	34.0	11.0	38.0
38-39	21.872505582532874	21.0	11.0	34.0	11.0	38.0
40-41	21.773925140892494	21.0	11.0	34.0	11.0	38.0
42-43	22.048603480664944	21.0	11.0	34.0	11.0	38.0
44-45	22.23307517810938	21.0	11.0	34.0	11.0	38.0
46-47	22.07880232516925	21.0	11.0	34.0	11.0	38.0
48-49	21.78202424414277	21.0	11.0	34.0	11.0	38.0
50-51	21.583693687307267	21.0	11.0	32.5	11.0	38.0
52-53	21.8903785488959	21.0	11.0	34.0	11.0	38.0
54-55	21.84692340410449	21.0	11.0	34.0	11.0	38.0
56-57	21.78849289334704	21.0	11.0	34.0	11.0	38.0
58-59	21.60598837415376	21.0	11.0	33.5	11.0	38.0
60-61	21.976712862864638	21.0	11.0	34.0	11.0	38.0
62-63	21.644472406337506	21.0	11.0	34.0	11.0	38.0
64-65	21.625137348031046	21.0	11.0	33.5	11.0	38.0
66-67	22.091571261475206	21.5	11.0	33.5	11.0	38.0
68-69	21.867011661290896	21.5	11.0	33.0	10.5	38.0
70-71	22.090720589799027	22.0	11.0	33.5	11.0	38.0
72-73	21.728139510154893	21.5	11.0	32.5	11.0	38.0
74-75	21.485529720341688	21.0	11.0	32.0	11.0	38.0
76	21.16194662035232	21.0	11.0	31.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
11	2.0
12	34.0
13	550.0
14	2304.0
15	4361.0
16	5860.0
17	6656.0
18	6543.0
19	5584.0
20	4250.0
21	2971.0
22	2003.0
23	1420.0
24	1037.0
25	758.0
26	558.0
27	486.0
28	406.0
29	361.0
30	372.0
31	417.0
32	468.0
33	600.0
34	797.0
35	1122.0
36	2160.0
37	4346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.914578385850497	20.483465069294297	8.50848899443519	51.09346755042002
2	9.474355793428561	37.95945131676886	26.542728529401337	26.02346436040123
3	9.075603445220288	21.42452061106582	31.398646014248747	38.10122992946514
4	9.327260482756177	36.162407400843584	23.758551022578246	30.751781093821997
5	10.147804203735866	30.0818771488321	36.02417325346472	23.746145393967318
6	13.33782298940205	39.9390351965406	23.460815935916067	23.262325878141283
7	25.50642445724413	25.03145768719539	15.197164377492248	34.264953478068236
8	12.85423128046079	40.54231280460789	22.741692512184315	23.86176340274701
9	20.003899050049625	19.376506451155535	15.54834822061534	45.0712462781795
10-11	13.59668944005813	33.2456070393705	22.729085254007497	30.428618266563877
12-13	13.341367454719455	22.901676532095134	26.45588912912487	37.30106688406054
14-15	27.1931379151455	27.716832665792364	18.133484563853543	26.95654485520859
16-17	29.93300960550101	23.341190231453584	16.16630631269273	30.559493850352677
18-19	19.214000886132034	23.243243243243246	21.47984049623394	36.06291537439078
20-21	25.969411264310775	22.51267146350973	17.973983624570234	33.54393364760926
22-23	30.450771371099943	21.373315256400033	15.846558736741368	32.329354635758655
24-25	18.756203034169857	32.468453140507584	14.96260456543315	33.812739259889405
26-27	24.160847836103923	33.0769503420409	17.48130294545068	25.280898876404496
28-29	19.782362115411882	30.234474691620587	21.950765631646107	28.032397561321424
30-31	21.907454010562507	26.744763052493532	22.88927090348421	28.458512033459755
32-33	16.627972920284975	31.786764966504805	22.553432814659907	29.031829298550317
34-35	15.350465219317677	37.866194062915376	18.98449268941072	27.798848028356225
36-37	24.039986883734947	25.056497425489866	20.69356682648422	30.20994886429097
38-39	25.724438615458233	21.257288694326782	25.721780125126276	27.296492565088705
40-41	18.629709708290505	29.325133803565734	22.186580654308298	29.858575833835467
42-43	12.655513415801225	28.188246553007478	25.035444653174068	34.12079537801723
44-45	12.712337506978228	22.792000070889934	31.38651850670353	33.109143915428305
46-47	16.09127159946832	28.099246787771374	25.44528134692069	30.364200265839607
48-49	17.540672739517245	29.518307163364405	21.967709920958423	30.973310176159924
50-51	26.02280883643034	28.380785283249594	16.11150986699276	29.484896013327305
52-53	11.521999202445834	32.99127121272542	21.692587177101334	33.79414240772741
54-55	15.275685447423923	25.825461248072596	21.76417418428655	37.13467912021694
56-57	19.214136329800432	29.407855091985397	18.00893268583177	33.369075892382405
58-59	16.76148951631485	29.485475266301552	19.209159710037042	34.543875507346556
60-61	12.362846760613312	26.45395728086502	21.665337233005406	39.51785872551626
62-63	17.08436724565757	27.757001063452673	19.407125132931586	35.751506557958166
64-65	17.363443795478112	26.519777716721766	18.832037862606242	37.284740625193876
66-67	13.332388025310623	37.16478491288396	14.564242542405928	34.93858451939949
68-69	15.302342891574805	41.23365115372346	12.606777017686882	30.857228937014852
70-71	12.47485622635156	42.608395140494984	15.507173175248779	29.40957545790467
72-73	15.208202256152134	41.848697794358735	15.11604207466747	27.82705787482166
74-75	17.71383502888944	40.9113466378363	13.452199496650245	27.922618836624014
76	14.231028249388581	45.87778683585581	12.13624924680112	27.754935667954488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	3.5
17	2.0
18	5.0
19	15.5
20	23.5
21	24.0
22	26.5
23	30.5
24	35.0
25	38.0
26	54.5
27	89.0
28	128.5
29	150.0
30	185.0
31	283.0
32	393.0
33	440.0
34	512.0
35	713.0
36	934.0
37	1026.0
38	1173.5
39	1518.0
40	1817.5
41	2158.0
42	2396.0
43	2574.0
44	2923.0
45	3239.5
46	3385.0
47	3513.0
48	3683.0
49	3637.5
50	3550.0
51	3499.0
52	3351.0
53	3124.0
54	2994.0
55	2776.5
56	2435.0
57	2139.5
58	1968.0
59	1810.0
60	1499.5
61	1204.5
62	1062.0
63	1000.5
64	831.0
65	674.5
66	552.0
67	478.0
68	424.0
69	318.5
70	233.0
71	199.0
72	188.5
73	154.5
74	123.0
75	115.0
76	95.0
77	75.0
78	62.0
79	49.0
80	47.0
81	31.0
82	22.0
83	27.0
84	21.5
85	15.5
86	10.0
87	5.0
88	5.5
89	6.0
90	5.0
91	3.0
92	2.0
93	3.5
94	3.0
95	2.0
96	3.0
97	2.0
98	1.5
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0017722326587034348
8	0.0017722326587034348
9	0.0035444653174068696
10-11	8.861163293517174E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0017722326587034348
20-21	0.0
22-23	8.861163293517174E-4
24-25	0.0035444653174068696
26-27	0.0
28-29	0.0035444653174068696
30-31	0.0
32-33	0.0
34-35	0.0017722326587034348
36-37	0.013291744940275761
38-39	0.005316697976110304
40-41	0.0
42-43	0.0
44-45	8.861163293517174E-4
46-47	0.0017722326587034348
48-49	0.0
50-51	8.861163293517174E-4
52-53	0.006202814305462021
54-55	0.005316697976110304
56-57	0.007088930634813739
58-59	0.008861163293517172
60-61	0.01949455924573778
62-63	0.010633395952220608
64-65	0.020380675575089498
66-67	0.012405628610924042
68-69	0.0
70-71	8.861163293517174E-4
72-73	0.004430581646758586
74-75	0.007088930634813739
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	56426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.09715379435012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.66101920770592	89.9886577109843
2	1.4638975081380519	2.7256938290858823
3	0.8014315356646552	2.238329847942438
4	0.5101749443185928	1.8998334101300818
5	0.2988711427538025	1.3912026370821962
6	0.15229102815480383	0.8506716761776487
7	0.06091641126192153	0.39698011554956936
8	0.02094001637128553	0.15595647396590223
9	0.011421827111610288	0.09570056356998546
>10	0.01903637851935048	0.25697373551199804
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012405628610924042	0.0
2	0.0	0.0	0.0	0.012405628610924042	0.0
3	0.0	0.0	0.0	0.012405628610924042	0.0
4	0.0	0.0	0.0	0.021266791904441217	0.0
5	0.0	0.0	0.0	0.03367242051536526	0.0
6	0.0	0.0	0.0	0.03367242051536526	0.0
7	0.0	0.0	0.0	0.03367242051536526	0.0
8	0.0017722326587034346	0.0	0.0	0.03367242051536526	0.0
9	0.0017722326587034346	0.0	0.0	0.03367242051536526	0.0
10	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
11	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
12	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
13	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
14	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
15	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
16	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
17	0.0017722326587034346	0.0	0.0	0.03898911849147556	0.0
18	0.0017722326587034346	0.0	0.0	0.040761351150178995	0.0
19	0.0017722326587034346	0.0	0.0	0.040761351150178995	0.0
20	0.0017722326587034346	0.0	0.0	0.06202814305462021	0.0
21	0.0017722326587034346	0.0	0.0	0.06202814305462021	0.0
22	0.0017722326587034346	0.0	0.0	0.10456172686350264	0.0
23	0.0017722326587034346	0.0	0.0	0.10633395952220608	0.0
24	0.0017722326587034346	0.0	0.0	0.1329174494027576	0.0
25	0.0017722326587034346	0.0	0.0	0.13646191472016447	0.0
26	0.0017722326587034346	0.0	0.0	0.14000638003757132	0.0
27	0.0017722326587034346	0.0	0.0	0.1683621025768263	0.0
28	0.0017722326587034346	0.0	0.0	0.1843121965051572	0.0
29	0.0017722326587034346	0.0	0.0	0.203806755750895	0.0
30	0.0017722326587034346	0.0	0.0	0.23216247829014994	0.0
31	0.0017722326587034346	0.0	0.0	0.2676071314642186	0.0
32	0.0017722326587034346	0.0	0.0	0.2764682947577358	0.0
33	0.0017722326587034346	0.0	0.0	0.29241838868606673	0.0
34	0.0017722326587034346	0.0017722326587034346	0.0	0.3952078828908659	0.0
35	0.0017722326587034346	0.0017722326587034346	0.0	0.4466026299932655	0.0
36	0.0017722326587034346	0.0017722326587034346	0.0	0.6061035692765746	0.0
37	0.0017722326587034346	0.0017722326587034346	0.0	0.7656045085598837	0.0
38	0.0017722326587034346	0.0017722326587034346	0.0	1.0208060114131783	0.0
39	0.0017722326587034346	0.0017722326587034346	0.0	1.2281572324814802	0.0
40	0.0017722326587034346	0.0017722326587034346	0.0	1.4408251515258923	0.0
41	0.0017722326587034346	0.0017722326587034346	0.0	1.6588097685464147	0.0
42	0.0017722326587034346	0.0017722326587034346	0.0	1.8572998263211995	0.0
43	0.0017722326587034346	0.0017722326587034346	0.0	2.1249069577854183	0.0
44	0.0017722326587034346	0.0017722326587034346	0.0	2.3730195300038988	0.0
45	0.0017722326587034346	0.0017722326587034346	0.0	2.6069542409527524	0.0
46	0.0017722326587034346	0.0017722326587034346	0.0	2.862155743806047	0.0
47	0.0017722326587034346	0.0017722326587034346	0.0	3.110268316024528	0.0
48	0.0017722326587034346	0.0017722326587034346	0.0	3.3495197249494915	0.0
49	0.0017722326587034346	0.0017722326587034346	0.0	3.5958600645092686	0.0
50	0.0017722326587034346	0.0017722326587034346	0.0	3.8156169141884946	0.0
51	0.0017722326587034346	0.0017722326587034346	0.0	4.026512600574203	0.0
52	0.0017722326587034346	0.0017722326587034346	0.0	4.32424768723638	0.0
53	0.0017722326587034346	0.0017722326587034346	0.0	4.547549002233013	0.0
54	0.0017722326587034346	0.0017722326587034346	0.0	4.774394782547053	0.0
55	0.0017722326587034346	0.0017722326587034346	0.0	4.930351256512955	0.0
56	0.0017722326587034346	0.0017722326587034346	0.0	5.187324992024953	0.0
57	0.0017722326587034346	0.0017722326587034346	0.0	5.337964768014745	0.0
58	0.0017722326587034346	0.0017722326587034346	0.0	5.460248821465282	0.0
59	0.0017722326587034346	0.0017722326587034346	0.0	5.612660830113778	0.0
60	0.0017722326587034346	0.0017722326587034346	0.0	5.77393400205579	0.0
61	0.0017722326587034346	0.0017722326587034346	0.0	5.864317867649665	0.0
62	0.0017722326587034346	0.0017722326587034346	0.0	6.025591039591678	0.0
63	0.0017722326587034346	0.0017722326587034346	0.0	6.1336972317725875	0.0
64	0.0017722326587034346	0.0017722326587034346	0.0	6.328642824229965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTC	15	0.0022139207	70.00001	50
AGGACAG	15	0.0022139207	70.00001	30
CACCTTG	15	0.0022139207	70.00001	1
GGCCTCT	15	0.0022139207	70.00001	25
TGGTCCA	15	0.0022139207	70.00001	49
GCTTAGG	20	7.863532E-5	70.0	30
TCGGCGG	45	3.7471182E-10	62.222225	45
AGGCTTA	35	2.9251532E-7	60.000004	28
TTACGTG	30	8.2776605E-6	58.333336	32
CTCGGCG	55	3.45608E-11	57.272724	44
CTTAAAG	25	2.3715281E-4	55.999996	42
GAGGATA	25	2.3715281E-4	55.999996	29
TCGGGGG	25	2.3715281E-4	55.999996	45
CCGTACC	25	2.3715281E-4	55.999996	55
AAGGCTT	50	9.531504E-10	55.999996	27
AGAGGAT	25	2.3715281E-4	55.999996	28
CTTAGGT	25	2.3715281E-4	55.999996	31
CTAGGTG	25	2.3715281E-4	55.999996	44
ACGAACG	25	2.3715281E-4	55.999996	24
GAAGGCT	45	2.6424459E-8	54.444447	26
>>END_MODULE