FastQCFastQC Report
Wed 25 May 2016
SRR1779791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56426
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3046453.98929571474144TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG39647.025130259100415TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC23124.09740190692234RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT7091.2565129550207352TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5340.9463722397476341RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT5010.8878885620104207No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT4460.7904157657817318No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT3100.5493921241980647No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2540.45014709531067243TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT2030.3597632297167972No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1960.3473576011058732TruSeq Adapter, Index 5 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC1940.3438131357884663RNA PCR Primer, Index 5 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG1410.24988480487718426No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1220.21621238436181903No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1120.19849005777478468TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT1080.19140112713997093No Hit
CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT970.17190656789423314No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCG920.16304540460071598No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC850.15063977598979195TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC840.1488675433310885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCC740.13114521674405416No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT720.12760075142664729No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCG700.12405628610924041No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT670.11873958813313011Illumina PCR Primer Index 5 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT650.11519512281572325RNA PCR Primer, Index 5 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTCTGCCGT640.11342289015701983No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT580.1027894942047992No Hit
CTTATACACATCTCCGAGCCCACGAGAAACAAAGTGATCTCGTATGCCGT570.10101726154609578Illumina PCR Primer Index 5 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAT150.002208687470.0417152
TGAACAA150.002208687470.0417159
GCTTGAC401.3096724E-1070.041756
CTTGACA401.3096724E-1070.041757
CTTTACA150.002216480269.9796141
TTATATA150.002216480269.9796142
TAATCTC150.002216480269.97961435
CTTATAT150.002216480269.9796141
AACGTGA207.874888E-569.9796131
GCGATCT207.874888E-569.9796134
CACAACG207.874888E-569.9796128
AGCGATC207.874888E-569.9796133
ACAACGT207.874888E-569.9796129
CAACGTG207.874888E-569.9796130
TCTTTAT501.2732926E-1162.9816471
GAAAAAC352.9130933E-760.03574460
CTATACA308.291965E-658.3163451
GAGCTCG308.291965E-658.31634536
TGAGCTC308.291965E-658.31634535
CGTCTTA252.3645404E-456.03336348
Produced by FastQC (version 0.11.5)