##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779790_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45545
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.131869579536723	32.0	21.0	34.0	12.0	34.0
2	26.49689318256669	33.0	21.0	34.0	12.0	34.0
3	26.345482489845207	33.0	21.0	34.0	11.0	34.0
4	26.930310681743332	33.0	21.0	34.0	12.0	34.0
5	26.15525304643759	34.0	11.0	34.0	11.0	34.0
6	29.304665715226697	36.0	21.0	38.0	11.0	38.0
7	29.480052695136678	36.0	21.0	38.0	12.0	38.0
8	29.01850916675815	34.0	21.0	38.0	11.0	38.0
9	29.479394005928203	36.0	21.0	38.0	11.0	38.0
10-11	28.4324404435174	34.0	21.0	38.0	11.0	38.0
12-13	28.949357778021735	35.5	21.0	38.0	11.0	38.0
14-15	27.90840926556153	33.5	16.0	38.0	11.0	38.0
16-17	28.24787572730267	34.5	16.5	38.0	11.0	38.0
18-19	28.655253046437586	35.0	21.0	38.0	11.0	38.0
20-21	28.081051707102866	32.5	16.0	38.0	11.0	38.0
22-23	28.588538807772533	35.0	21.0	38.0	11.0	38.0
24-25	28.889021846525416	35.5	21.0	38.0	11.0	38.0
26-27	27.98724338566253	34.0	16.0	38.0	11.0	38.0
28-29	28.675760237128117	35.0	21.0	38.0	11.0	38.0
30-31	28.85794269403886	37.0	21.0	38.0	11.0	38.0
32-33	29.00626852563399	37.0	21.0	38.0	11.0	38.0
34-35	29.11287737402569	36.5	21.0	38.0	11.0	38.0
36-37	28.778416950268962	35.5	21.0	38.0	11.0	38.0
38-39	28.660489625644967	35.5	21.0	38.0	11.0	38.0
40-41	28.66387089691514	35.5	21.0	38.0	11.0	38.0
42-43	28.909155779997803	36.5	21.0	38.0	11.0	38.0
44-45	29.00703699637721	36.0	21.0	38.0	11.0	38.0
46-47	28.889065759139314	36.0	21.0	38.0	11.0	38.0
48-49	28.669030629048194	35.5	21.0	38.0	11.0	38.0
50-51	28.209561971676365	35.0	16.0	38.0	11.0	38.0
52-53	28.71944230980349	35.5	21.0	38.0	11.0	38.0
54-55	28.746020419365465	36.0	21.0	38.0	11.0	38.0
56-57	28.744834778790207	36.0	21.0	38.0	11.0	38.0
58-59	28.78252277966846	36.0	21.0	38.0	11.0	38.0
60-61	28.849017455264026	36.0	21.0	38.0	11.0	38.0
62-63	28.73351630255791	36.0	21.0	38.0	11.0	38.0
64-65	28.74790866176309	36.0	20.0	38.0	11.0	38.0
66-67	28.821374464815015	36.0	21.0	38.0	11.0	38.0
68-69	28.4139532330662	34.0	21.0	38.0	11.0	38.0
70-71	28.34045449555385	34.0	20.5	38.0	11.0	38.0
72-73	28.362959710176746	34.5	19.5	38.0	11.0	38.0
74-75	28.481304204632778	35.5	20.0	38.0	11.0	38.0
76	27.73430672960808	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	40.0
13	342.0
14	1230.0
15	2039.0
16	2654.0
17	2841.0
18	2743.0
19	2163.0
20	1656.0
21	1155.0
22	759.0
23	564.0
24	415.0
25	353.0
26	351.0
27	347.0
28	418.0
29	467.0
30	552.0
31	675.0
32	817.0
33	1222.0
34	1786.0
35	2806.0
36	5340.0
37	11810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.537819738719946	21.418377428916457	16.5111428257767	32.532660006586894
2	22.38225930398507	21.637940498408167	32.02546931606104	23.954330881545722
3	14.816115929300691	29.241409594906138	31.37556263036557	24.5669118454276
4	11.344823800636734	27.57053463607421	26.101657701174663	34.982983862114395
5	20.498408167746184	31.050609287517837	29.287517839499394	19.16346470523658
6	22.50301899220551	43.63157316939291	14.21012185750357	19.655285980898014
7	19.699198594796353	36.032495334284775	16.041277857064443	28.227028213854428
8	17.218135909540017	45.39686024810627	15.360632341640137	22.02437150071358
9	20.077287897417882	22.580361847883363	16.693746706481644	40.64860354821711
10-11	19.355794881928663	31.62401607219313	21.98399367651418	27.03619536936403
12-13	18.56535915423377	25.372986859006026	25.082062598118327	30.979591388641875
14-15	25.199253485563727	27.5496761444725	20.315073004720606	26.935997365243164
16-17	26.688988912064993	26.306949171149412	19.92425074102536	27.079811175760238
18-19	20.904829342730736	26.381890239216588	23.017049259515417	29.696231158537255
20-21	24.696454056427708	25.41113184762323	21.551213085958942	28.34120100999012
22-23	26.418926336590186	25.13667801075859	20.275551652212098	28.168844000439126
24-25	20.463502728101087	30.9323848104601	19.7652845019706	28.83882795946821
26-27	23.705126797672634	30.431441431551214	21.42276869030629	24.440663080469864
28-29	21.436883824078343	28.836484201743406	23.688601980545858	26.03802999363239
30-31	22.149522450323854	27.895487978921945	23.91700515973213	26.037984411022062
32-33	19.36656054451641	30.223954330881547	23.57778021736744	26.831704907234606
34-35	18.345995081679256	32.11839100649921	23.04584577551379	26.489768136307745
36-37	23.41838863391818	26.688113485144598	23.40631107402446	26.487186806912753
38-39	23.75933245498463	24.89020641194554	25.665349143610012	25.685111989459813
40-41	20.61170942704388	28.56546893697373	24.00070260953573	26.82211902644666
42-43	17.460944790260076	27.81235934086443	25.723193799470845	29.003502069404647
44-45	17.267387604984354	25.169896250754793	28.58868090245375	28.974035241807105
46-47	19.202055245707257	27.155153484695443	26.12862851872996	27.51416275086733
48-49	19.37994708471934	28.293207741878824	24.915192833382736	27.411652340019106
50-51	24.104692268844826	27.199569637485453	21.849680521704762	26.846057571964955
52-53	15.762892370527332	30.20059068301841	24.425511358021982	29.611005588432278
54-55	17.897059792275094	27.01411914538548	24.12002371489427	30.96879734744516
56-57	19.394365269329587	28.167065592129823	23.062649597048683	29.375919541491907
58-59	18.583099086437105	28.216136683063947	23.55718552354181	29.643578706957136
60-61	16.58175030196552	26.856264411990775	24.261557044032063	32.30042824201164
62-63	18.920462031709782	27.20914401159471	22.89845843032193	30.971935526373578
64-65	19.162814191748932	26.264179120867055	22.952331799663984	31.62067488772003
66-67	17.35292179985506	31.152689022113883	20.432834837604585	31.06155434042647
68-69	18.414956800491826	33.386029048512995	19.30639264894773	28.89262150204745
70-71	19.095609788229094	31.890788130290154	19.195512081590532	29.81808999989022
72-73	20.292018882423974	31.42386650565375	19.364364913821493	28.919749698100777
74-75	18.38034277181849	30.275249503189468	20.4762793557383	30.86812836925374
76	17.773630475354047	30.209682731364584	20.469864968712262	31.54682182456911
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	5.5
17	10.0
18	19.5
19	29.5
20	34.5
21	39.0
22	60.0
23	101.5
24	141.5
25	161.0
26	206.0
27	288.5
28	335.0
29	344.0
30	382.0
31	519.5
32	647.5
33	676.0
34	758.5
35	974.0
36	1177.5
37	1248.0
38	1294.5
39	1410.0
40	1675.5
41	1945.0
42	2018.0
43	2055.0
44	2194.5
45	2390.5
46	2484.0
47	2474.0
48	2479.0
49	2416.5
50	2339.0
51	2246.0
52	2128.5
53	2010.5
54	1917.0
55	1832.0
56	1719.0
57	1546.0
58	1401.0
59	1330.0
60	1163.5
61	973.5
62	879.0
63	800.0
64	668.5
65	536.5
66	427.0
67	397.0
68	386.0
69	354.5
70	293.0
71	252.0
72	224.0
73	175.0
74	142.0
75	130.0
76	126.0
77	102.5
78	77.0
79	71.0
80	58.5
81	52.5
82	44.0
83	29.0
84	25.0
85	23.5
86	17.5
87	9.0
88	9.0
89	9.5
90	5.0
91	5.0
92	10.0
93	6.5
94	4.0
95	3.5
96	2.0
97	1.5
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002195630694917115
10-11	0.0010978153474585574
12-13	0.0010978153474585574
14-15	0.0
16-17	0.0
18-19	0.0010978153474585574
20-21	0.0
22-23	0.0
24-25	0.0010978153474585574
26-27	0.0
28-29	0.00439126138983423
30-31	0.0
32-33	0.0
34-35	0.002195630694917115
36-37	0.01317378416950269
38-39	0.010978153474585575
40-41	0.0010978153474585574
42-43	0.0010978153474585574
44-45	0.0054890767372927874
46-47	0.006586892084751345
48-49	0.0010978153474585574
50-51	0.00439126138983423
52-53	0.009880338127127016
54-55	0.00878252277966846
56-57	0.01317378416950269
58-59	0.019760676254254032
60-61	0.02195630694917115
62-63	0.015369414864419803
64-65	0.025249752991546824
66-67	0.01756504555933692
68-69	0.0010978153474585574
70-71	0.0010978153474585574
72-73	0.0
74-75	0.009880338127127016
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	45545.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.28883521791634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.54828079842261	71.16697771434845
2	6.498662993274451	10.565374903941157
3	3.2277233071334037	7.871336041277857
4	1.5746968100910246	5.120210780546712
5	0.7292763957539907	2.9641014381381052
6	0.2349890608540637	1.146119222746734
7	0.11614401858304298	0.6608848391700516
8	0.04591740269562164	0.29860577450872766
9	0.01620614212786646	0.11856405752552421
>10	0.00810307106393323	0.0878252277966846
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.002195630694917115	0.0
5	0.0	0.0	0.0	0.019760676254254036	0.0
6	0.0	0.0	0.0	0.019760676254254036	0.0
7	0.0	0.0	0.0	0.019760676254254036	0.0
8	0.0	0.0	0.0	0.02195630694917115	0.0
9	0.0	0.0	0.0	0.02195630694917115	0.0
10	0.0	0.0	0.0	0.02195630694917115	0.0
11	0.0	0.0	0.0	0.024151937644088264	0.0
12	0.0	0.0	0.0	0.024151937644088264	0.0
13	0.0	0.0	0.0	0.024151937644088264	0.0
14	0.0	0.0	0.0	0.028543199033922495	0.0
15	0.0	0.0	0.0	0.03073882972883961	0.0
16	0.0	0.0	0.0	0.03073882972883961	0.0
17	0.0	0.0	0.0	0.03073882972883961	0.0
18	0.0	0.0	0.0	0.03073882972883961	0.0
19	0.0	0.0	0.0	0.04830387528817653	0.0
20	0.0	0.0	0.0	0.059282028762762104	0.0
21	0.0	0.0	0.0	0.06147765945767922	0.0
22	0.0	0.0	0.0	0.1163684268306071	0.0
23	0.0	0.0	0.0	0.12075968822044132	0.0
24	0.0	0.0	0.0	0.12075968822044132	0.0
25	0.0	0.0	0.0	0.12075968822044132	0.0
26	0.0	0.0	0.0	0.12295531891535844	0.0
27	0.0	0.0	0.0	0.1295422110001098	0.0
28	0.0	0.0	0.0	0.15149851794928093	0.0
29	0.0	0.0	0.0	0.18223734767812055	0.0
30	0.0	0.0	0.0	0.18662860906795478	0.0
31	0.0	0.0	0.0	0.20858491601712592	0.0
32	0.0	0.0	0.0	0.248106268525634	0.0
33	0.0	0.0	0.0	0.2788450982544736	0.0
34	0.0	0.0	0.0	0.34910528049182127	0.0
35	0.0	0.0	0.0	0.43473487759358875	0.0
36	0.0	0.0	0.0	0.5884290262377868	0.0
37	0.0	0.0	0.0	0.801405203644747	0.0
38	0.0	0.0	0.0	1.0473158414754637	0.0
39	0.0	0.0	0.0	1.3283565704248546	0.0
40	0.0	0.0	0.0	1.7213744648150182	0.0
41	0.0	0.0	0.0	2.195630694917115	0.0
42	0.0	0.0	0.0	2.5666922823581073	0.0
43	0.0	0.0	0.0	3.0453397738500385	0.0
44	0.0	0.0	0.0	3.515204742562301	0.0
45	0.0	0.0	0.0	4.1146119222746735	0.0
46	0.0	0.0	0.0	4.678889010868372	0.0
47	0.0	0.0	0.0	5.133384564716215	0.0
48	0.0	0.0	0.0	5.627401471072566	0.0
49	0.0	0.0	0.0	6.090679547700077	0.0
50	0.0	0.0	0.0	6.532001317378417	0.0
51	0.0	0.0	0.0	7.052365792073773	0.0
52	0.0	0.0	0.0	7.54199143704029	0.0
53	0.0	0.0	0.0	7.976726314633878	0.0
54	0.0	0.0	0.0	8.45537380612581	0.0
55	0.0	0.0	0.0	8.88132616093973	0.0
56	0.0	0.0	0.0	9.33801734548249	0.0
57	0.0	0.0	0.0	9.750795916126908	0.0
58	0.0	0.0	0.0	10.095509935228895	0.0
59	0.0	0.0	0.0	10.48633219892414	0.0
60	0.0	0.0	0.0	10.918871445822813	0.0
61	0.0	0.0	0.0	11.34701943133165	0.0
62	0.0	0.0	0.0	11.68075529695905	0.0
63	0.0	0.0	0.0	12.036447469535624	0.0
64	0.0	0.0	0.0	12.425074102535953	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	15	0.002204678	70.051704	32
AGCGTAG	15	0.002204678	70.051704	35
TGCTAGC	15	0.002204678	70.051704	36
CTTGTCA	20	7.861244E-5	69.974724	1
GAAGACG	15	0.0022143282	69.974724	14
CCGGAGT	15	0.0022143282	69.974724	27
TAGCCCG	20	7.861244E-5	69.974724	5
ACGGGAG	15	0.0022143282	69.974724	3
GTGCTAC	15	0.0022143282	69.974724	46
TCATCGT	15	0.0022143282	69.974724	5
GTGACGC	15	0.0022143282	69.974724	14
GCCCGCT	15	0.0022143282	69.974724	7
CTTACGC	20	7.861244E-5	69.974724	12
TGCCGCT	15	0.0022143282	69.974724	13
ACGAAGG	15	0.0022143282	69.974724	24
TCTCGGT	50	1.2732926E-11	62.97725	43
CTCGGTG	45	3.7289283E-10	62.199757	44
TGTGTAG	30	8.215824E-6	58.376423	35
GGAGTAG	25	2.357984E-4	56.04136	35
ATATGTA	25	2.3708568E-4	55.97978	26
>>END_MODULE