FastQCFastQC Report
Wed 25 May 2016
SRR1779790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45545
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1169825.68448786914041No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG17253.7874629487320233No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC8961.9672851026457348No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT4571.0034032275771214RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2850.6257547480513778No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1700.3732572181359095No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1580.34690964979690414No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT1470.3227577121528159No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT1260.2766494675595565No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1120.24591063783071684RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC720.15808541003403226No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG720.15808541003403226No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG630.13832473377977825No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG630.13832473377977825No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC600.1317378416950269No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG560.12295531891535842No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAA150.002198343470.10242569
TGAACCG150.002198343470.10242553
GTAGCGG150.002198343470.10242564
CGGGCTA150.002198343470.10242568
GTTCACT150.002198343470.10242556
TGTAGCG150.002198343470.10242563
GAACCGG150.002198343470.10242554
GTCGGCG150.002198343470.10242569
TCCGGTG150.002198343470.10242548
CGTCTTA252.7730712E-670.1024248
GTCTTAT252.7730712E-670.1024249
CTCCGGT150.002207976570.025347
CCTTATA150.002217641369.948361
AATTTCG150.002217641369.9483627
AGGACTC150.002217641369.9483643
TAGCAGG150.002217641369.9483639
GTAGCAC150.002217641369.9483627
CGAGAAT150.002217641369.9483623
GAATTTC150.002217641369.9483626
TTCGCCA150.002217641369.9483630
Produced by FastQC (version 0.11.5)