##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779790_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45545
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.45341969480733	34.0	34.0	34.0	34.0	34.0
2	33.584674497749475	34.0	34.0	34.0	34.0	34.0
3	33.73861016577012	34.0	34.0	34.0	34.0	34.0
4	33.50341420573059	34.0	34.0	34.0	34.0	34.0
5	33.531013283565706	34.0	34.0	34.0	34.0	34.0
6	37.1735206938193	38.0	38.0	38.0	36.0	38.0
7	37.25464924799649	38.0	38.0	38.0	36.0	38.0
8	37.11237237896586	38.0	38.0	38.0	36.0	38.0
9	37.01150510484137	38.0	38.0	38.0	36.0	38.0
10-11	36.965528598089804	38.0	37.0	38.0	35.0	38.0
12-13	37.1562849928642	38.0	37.5	38.0	36.0	38.0
14-15	37.1938632122077	38.0	38.0	38.0	36.0	38.0
16-17	37.01430453397738	38.0	38.0	38.0	35.0	38.0
18-19	36.81228455373806	38.0	37.0	38.0	34.0	38.0
20-21	36.9600834339664	38.0	37.5	38.0	35.0	38.0
22-23	36.9364584476891	38.0	37.5	38.0	35.0	38.0
24-25	37.095740476451866	38.0	38.0	38.0	36.0	38.0
26-27	36.91534745855748	38.0	38.0	38.0	35.0	38.0
28-29	36.3289823251729	38.0	37.0	38.0	34.0	38.0
30-31	36.78798990009881	38.0	37.0	38.0	34.5	38.0
32-33	36.72374574596553	38.0	37.0	38.0	34.5	38.0
34-35	36.62968492699528	38.0	37.0	38.0	34.0	38.0
36-37	36.50496212537051	38.0	37.0	38.0	34.0	38.0
38-39	35.12881765287079	38.0	36.0	38.0	29.0	38.0
40-41	36.08140300801405	38.0	37.0	38.0	33.0	38.0
42-43	36.87523328576134	38.0	37.0	38.0	35.0	38.0
44-45	36.60816774618509	38.0	37.0	38.0	34.5	38.0
46-47	36.78950488527829	38.0	37.0	38.0	34.5	38.0
48-49	36.3150730047206	38.0	37.0	38.0	34.0	38.0
50-51	36.414304533977386	38.0	37.0	38.0	34.0	38.0
52-53	36.3450982544736	38.0	37.0	38.0	34.0	38.0
54-55	36.37438796794379	38.0	37.0	38.0	34.0	38.0
56-57	36.20767372927874	38.0	37.0	38.0	34.0	38.0
58-59	36.27897683609617	38.0	37.0	38.0	34.0	38.0
60-61	35.76933801734548	38.0	37.0	38.0	31.5	38.0
62-63	35.624678889010866	38.0	37.0	38.0	31.0	38.0
64-65	36.18205071906905	38.0	37.0	38.0	34.0	38.0
66-67	35.84218904380283	38.0	36.5	38.0	32.5	38.0
68-69	35.696179602590846	38.0	37.0	38.0	31.0	38.0
70-71	33.48889010868372	37.5	32.0	38.0	19.5	38.0
72-73	29.570139422549126	36.5	22.5	38.0	11.0	38.0
74-75	28.94677791195521	36.5	21.0	38.0	11.0	38.0
76	28.87706663739159	36.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	4.0
24	9.0
25	39.0
26	69.0
27	164.0
28	246.0
29	436.0
30	739.0
31	1047.0
32	1684.0
33	2668.0
34	4596.0
35	9061.0
36	8551.0
37	16228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.487644151565075	13.590334980779792	12.953322350356947	48.968698517298186
2	12.220880447908662	12.96739488418048	54.950049401690634	19.86167526622022
3	12.007904270501701	15.336480403996047	53.53606323416401	19.11955209133824
4	8.6595674607531	40.72894939071248	30.30409485124602	20.307388297288394
5	9.028433417499176	27.392688549785927	53.65682292238445	9.922055110330442
6	18.36425513228675	54.216708749588314	15.900757492589745	11.518278625535185
7	16.583598638708967	24.911625864529586	16.695575804149744	41.809199692611706
8	14.563618399385222	53.964211219672855	13.845647162147326	17.6265232187946
9	14.330881545724008	16.513338456471622	13.397738500384234	55.75804149742013
10-11	15.696563837962454	34.22329564167307	31.527061148314857	18.553079372049623
12-13	15.748161159293005	15.57141288835218	35.15863431770776	33.52179163464705
14-15	14.419804588868152	15.133384564716215	18.536612141837743	51.910198704577894
16-17	31.951915687781312	31.54682182456911	15.270611483148535	21.23065100450104
18-19	33.16500164672302	20.744318805576903	15.751454605335383	30.339224942364694
20-21	16.359644307827423	16.496871226259742	15.292567790097705	51.85091667581513
22-23	14.90174552640246	33.936765835986385	15.462729168953782	35.69875946865737
24-25	32.39433527280711	33.75782193435064	15.340871665385883	18.50697112745636
26-27	32.81809199692612	35.560434734877596	16.733999341310792	14.887473926885498
28-29	15.545065320013174	20.05269513667801	31.967285102645736	32.434954440663084
30-31	14.417608958173236	16.302557909759578	53.04204632780767	16.237786804259525
32-33	29.503787462948733	16.43100230541223	22.58864858930728	31.476561642331756
34-35	18.26545175101548	16.9853990558788	33.72378965857943	31.02535953452629
36-37	13.82698430124053	48.281918981227356	18.52234054232078	19.36875617521133
38-39	14.494456032495334	22.307607860357887	33.214403337358654	29.983532769788123
40-41	14.299044900647711	15.719617960259086	35.9787023822593	34.0026347568339
42-43	31.65764598680382	15.739897021528867	34.30456596440765	18.297891027259652
44-45	16.00614776594577	32.16269623449336	36.230102096827316	15.601053902733561
46-47	31.183444944560325	16.718629926446372	21.839938522340542	30.25798660665276
48-49	30.040619167855965	14.8688110659787	18.36974420902404	36.72082555714129
50-51	17.207606833853045	15.011638631472616	35.59993851289033	32.180816021784004
52-53	13.313053886954899	14.938338037139939	52.15075608657933	19.597851989325836
54-55	12.735621072982116	15.210466189199877	39.05817478799596	32.99573794982204
56-57	30.224204409390005	15.420781474849779	22.381993343073393	31.973020772686827
58-59	14.362280480170453	16.444629932675095	49.89840858420006	19.294681002954388
60-61	29.238000065888453	32.41821596037908	22.528359487387853	15.815424486344618
62-63	13.860701107011069	53.73945703742752	16.306448778773504	16.09339307678791
64-65	12.285074331891346	55.64571027031775	15.700827861832714	16.36838753595819
66-67	12.530615382925678	55.70846467286845	15.620160572878342	16.140759371327526
68-69	12.327218031904966	55.61081650802016	15.531987308279263	16.529978151795614
70-71	13.052118444022353	51.87141116149362	16.03956917469066	19.03690121979337
72-73	14.381553664562174	44.06368377710678	17.525116662091683	24.029645896239362
74-75	15.417636467436651	40.16622019234992	18.50819024197444	25.90795309823899
76	14.714213565797852	41.18267056059375	17.92889923365758	26.174216639950814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	3.5
16	5.0
17	5.0
18	16.0
19	33.0
20	38.5
21	38.0
22	44.0
23	74.5
24	130.0
25	161.0
26	190.5
27	253.0
28	294.0
29	302.0
30	361.5
31	481.0
32	597.5
33	654.0
34	689.5
35	760.5
36	899.0
37	1002.0
38	1101.5
39	2757.5
40	5555.0
41	6410.5
42	6025.0
43	5068.5
44	3347.5
45	2152.5
46	1722.0
47	1563.5
48	1322.5
49	1180.5
50	1121.0
51	1038.0
52	948.0
53	918.5
54	896.0
55	877.0
56	851.0
57	803.5
58	763.0
59	684.5
60	550.5
61	466.5
62	438.0
63	417.0
64	380.5
65	351.0
66	307.5
67	278.0
68	268.5
69	253.0
70	226.5
71	206.0
72	196.0
73	160.5
74	130.5
75	126.0
76	98.5
77	65.5
78	60.0
79	60.0
80	41.5
81	31.0
82	34.0
83	29.0
84	21.5
85	15.0
86	10.0
87	4.0
88	3.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0439126138983423
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0032934460423756726
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015369414864419803
52-53	0.031836645076298166
54-55	0.059282028762762104
56-57	0.06257547480513777
58-59	0.04281479855088374
60-61	0.029641014381381052
62-63	0.03732572181359096
64-65	0.01317378416950269
66-67	0.045010429245800856
68-69	0.007684707432209901
70-71	0.009880338127127016
72-73	0.01646723021187836
74-75	0.006586892084751345
76	0.00878252277966846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	45545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.013283565704246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.69734513274336	16.96344274892963
2	18.16637168141593	11.267976726314634
3	11.66725663716814	10.855198155670216
4	7.136283185840709	8.852782961905808
5	4.127433628318584	6.40026347568339
6	1.9964601769911505	3.715007135799758
7	0.9203539823008848	1.9980239323745745
8	0.5380530973451327	1.334943462509606
9	0.16991150442477876	0.47425623010209683
>10	0.4672566371681416	2.656713140849709
>50	0.04247787610619469	0.8475134482380063
>100	0.049557522123893805	3.1946426611044023
>500	0.007079646017699115	1.9672851026457348
>1k	0.007079646017699115	3.7874629487320233
>5k	0.0	0.0
>10k+	0.007079646017699115	25.68448786914041
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	11698	25.68448786914041	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	1725	3.7874629487320233	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	896	1.9672851026457348	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	457	1.0034032275771214	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	285	0.6257547480513778	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	170	0.3732572181359095	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	158	0.34690964979690414	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	147	0.3227577121528159	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	126	0.2766494675595565	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	112	0.24591063783071684	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	72	0.15808541003403226	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	72	0.15808541003403226	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	63	0.13832473377977825	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG	63	0.13832473377977825	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	60	0.1317378416950269	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	56	0.12295531891535842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006586892084751344	0.0
5	0.0	0.0	0.0	0.028543199033922495	0.0
6	0.0	0.0	0.0	0.028543199033922495	0.0
7	0.0	0.0	0.0	0.028543199033922495	0.0
8	0.0	0.0	0.0	0.03293446042375672	0.0
9	0.0	0.0	0.0	0.03293446042375672	0.0
10	0.0	0.0	0.0	0.03293446042375672	0.0
11	0.0	0.0	0.0	0.03732572181359095	0.0
12	0.0	0.0	0.0	0.03732572181359095	0.0
13	0.0	0.0	0.0	0.03732572181359095	0.0
14	0.0	0.0	0.0	0.03952135250850807	0.0
15	0.0	0.0	0.0	0.041716983203425186	0.0
16	0.0	0.0	0.0	0.041716983203425186	0.0
17	0.0	0.0	0.0	0.041716983203425186	0.0
18	0.0	0.0	0.0	0.041716983203425186	0.0
19	0.0	0.0	0.0	0.052695136678010755	0.0
20	0.0	0.0	0.0	0.06147765945767922	0.0
21	0.0	0.0	0.0	0.06147765945767922	0.0
22	0.0	0.0	0.0	0.0746514436271819	0.0
23	0.0	0.0	0.0	0.09002085849160171	0.0
24	0.0	0.0	0.0	0.09002085849160171	0.0
25	0.0	0.0	0.0	0.09002085849160171	0.0
26	0.0	0.0	0.0	0.09221648918651883	0.0
27	0.0	0.0	0.0	0.10099901196618728	0.0
28	0.0	0.0	0.0	0.12295531891535844	0.0
29	0.0	0.0	0.0	0.15149851794928093	0.0
30	0.0	0.0	0.0	0.15588977933911516	0.0
31	0.0	0.0	0.0	0.18004171698320343	0.0
32	0.0	0.0	0.0	0.22614996157646283	0.0
33	0.0	0.0	0.0	0.26786694477988804	0.0
34	0.0	0.0	0.0	0.34251838840706994	0.0
35	0.0	0.0	0.0	0.4259523548139203	0.0
36	0.0	0.0	0.0	0.5950159183225382	0.0
37	0.0	0.0	0.0	0.8672741244922604	0.0
38	0.0	0.0	0.0	1.108793500933143	0.0
39	0.0	0.0	0.0	1.3898342298825337	0.0
40	0.0	0.0	0.0	1.778460862882863	0.0
41	0.0	0.0	0.0	2.239543308815457	0.0
42	0.0	0.0	0.0	2.6062136348666156	0.0
43	0.0	0.0	0.0	3.0826654956636292	0.0
44	0.0	0.0	0.0	3.5591173564606433	0.0
45	0.0	0.0	0.0	4.145350752003513	0.0
46	0.0	0.0	0.0	4.711823471292129	0.0
47	0.0	0.0	0.0	5.1663190251399715	0.0
48	0.0	0.0	0.0	5.6581403008014055	0.0
49	0.0	0.0	0.0	6.14117905368317	0.0
50	0.0	0.0	0.0	6.578109561971677	0.0
51	0.0	0.0	0.0	7.10066966736195	0.0
52	0.0	0.0	0.0	7.585904050938632	0.0
53	0.0	0.0	0.0	7.992095729498298	0.0
54	0.0	0.0	0.0	8.450982544735975	0.0
55	0.0	0.0	0.0	8.887913053024482	0.0
56	0.0	0.0	0.0	9.327039192007904	0.0
57	0.0	0.0	0.0	9.73323087056757	0.0
58	0.0	0.0	0.0	10.066966736194972	0.0
59	0.0	0.0	0.0	10.453397738500385	0.0
60	0.0	0.0	0.0	10.879350093314304	0.0
61	0.0	0.0	0.0	11.292128663958723	0.0
62	0.0	0.0	0.0	11.619277637501373	0.0
63	0.0	0.0	0.0	11.98594796355253	0.0
64	0.0	0.0	0.0	12.387748380722362	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCTAA	15	0.0021983434	70.102425	69
TGAACCG	15	0.0021983434	70.102425	53
GTAGCGG	15	0.0021983434	70.102425	64
CGGGCTA	15	0.0021983434	70.102425	68
GTTCACT	15	0.0021983434	70.102425	56
TGTAGCG	15	0.0021983434	70.102425	63
GAACCGG	15	0.0021983434	70.102425	54
GTCGGCG	15	0.0021983434	70.102425	69
TCCGGTG	15	0.0021983434	70.102425	48
CGTCTTA	25	2.7730712E-6	70.10242	48
GTCTTAT	25	2.7730712E-6	70.10242	49
CTCCGGT	15	0.0022079765	70.0253	47
CCTTATA	15	0.0022176413	69.94836	1
AATTTCG	15	0.0022176413	69.94836	27
AGGACTC	15	0.0022176413	69.94836	43
TAGCAGG	15	0.0022176413	69.94836	39
GTAGCAC	15	0.0022176413	69.94836	27
CGAGAAT	15	0.0022176413	69.94836	23
GAATTTC	15	0.0022176413	69.94836	26
TTCGCCA	15	0.0022176413	69.94836	30
>>END_MODULE