##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779789_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123579
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.699754812710896	34.0	32.0	34.0	21.0	34.0
2	31.34959823270944	34.0	33.0	34.0	23.0	34.0
3	31.11529466980636	34.0	33.0	34.0	23.0	34.0
4	31.24831888913165	34.0	33.0	34.0	23.0	34.0
5	31.85931266639154	34.0	34.0	34.0	23.0	34.0
6	34.46790312269884	38.0	35.0	38.0	24.0	38.0
7	34.61217520776184	38.0	36.0	38.0	25.0	38.0
8	33.79592811076316	38.0	34.0	38.0	21.0	38.0
9	34.92098172019518	38.0	36.0	38.0	27.0	38.0
10-11	33.539788313548414	37.5	32.5	38.0	19.0	38.0
12-13	34.56337646363865	38.0	36.0	38.0	25.0	38.0
14-15	33.17797926832229	37.5	32.5	38.0	16.0	38.0
16-17	33.97836606543183	38.0	35.0	38.0	19.0	38.0
18-19	34.96781815680657	38.0	36.5	38.0	26.5	38.0
20-21	33.51694867250909	38.0	33.0	38.0	18.5	38.0
22-23	34.508702934964674	38.0	36.0	38.0	24.0	38.0
24-25	34.87741444743848	38.0	36.5	38.0	26.0	38.0
26-27	33.70865195542932	38.0	34.0	38.0	21.5	38.0
28-29	35.150543377111	38.0	36.5	38.0	28.5	38.0
30-31	35.52598337905307	38.0	37.0	38.0	31.0	38.0
32-33	35.60623568729315	38.0	37.0	38.0	31.0	38.0
34-35	35.593211629807655	38.0	37.0	38.0	31.0	38.0
36-37	35.07731086996982	38.0	37.0	38.0	26.0	38.0
38-39	35.05948421657401	38.0	36.5	38.0	27.5	38.0
40-41	35.049632219066346	38.0	36.5	38.0	26.5	38.0
42-43	35.38448280047581	38.0	37.0	38.0	29.0	38.0
44-45	35.44411671886	38.0	37.0	38.0	30.5	38.0
46-47	35.3701923465961	38.0	37.0	38.0	29.0	38.0
48-49	35.14425590108351	38.0	36.5	38.0	28.5	38.0
50-51	34.43947596274448	38.0	35.5	38.0	24.0	38.0
52-53	35.04234942830092	38.0	36.5	38.0	27.0	38.0
54-55	35.21319965366284	38.0	37.0	38.0	29.0	38.0
56-57	35.22348457262156	38.0	37.0	38.0	27.0	38.0
58-59	35.451541119445864	38.0	37.0	38.0	30.5	38.0
60-61	35.33875496645871	38.0	37.0	38.0	27.5	38.0
62-63	35.34477540682478	38.0	37.0	38.0	27.5	38.0
64-65	35.39372385275816	38.0	37.0	38.0	29.0	38.0
66-67	35.12016200163458	38.0	37.0	38.0	27.0	38.0
68-69	34.454429959782814	38.0	36.0	38.0	23.5	38.0
70-71	34.47754472847328	38.0	35.5	38.0	24.0	38.0
72-73	34.88821725374052	38.0	36.0	38.0	26.0	38.0
74-75	34.995666739494574	38.0	36.5	38.0	26.5	38.0
76	34.04127723966046	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	10.0
13	163.0
14	608.0
15	966.0
16	1215.0
17	1195.0
18	1148.0
19	975.0
20	783.0
21	576.0
22	473.0
23	505.0
24	512.0
25	592.0
26	690.0
27	882.0
28	1155.0
29	1399.0
30	1997.0
31	2630.0
32	3563.0
33	5188.0
34	7725.0
35	12784.0
36	25221.0
37	50623.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.60027998284498	21.06668608744204	9.544501897571594	26.788532032141383
2	22.019113279764362	21.205059111985047	30.388658267181317	26.387169341069278
3	19.73798137223962	21.212341902750467	30.554544056838136	28.495132668171774
4	13.652805088243147	19.31477030887125	34.984099240162166	32.04832536272344
5	14.887642722469026	32.861570331528824	35.2932132482056	16.95757369779655
6	27.548369868667006	37.17945605644972	18.664174333826942	16.60799974105633
7	27.766358619127	29.538576075873568	22.057228866913754	20.63783643808568
8	24.579405219502327	34.14444669229213	18.7667408456403	22.509407242565242
9	24.545189693124435	18.002233587983945	20.413051922827915	37.0395247960637
10-11	23.96874785050435	24.88640547681377	25.471068294281636	25.673778378400247
12-13	24.207996504260432	23.468793241570168	26.16463962323696	26.158570630932438
14-15	23.74037555076328	24.355369259215802	23.627087236048343	28.277167953972576
16-17	24.25331164680083	26.896964694648766	24.65103294249023	24.198690716060174
18-19	22.41342337275593	26.711200844827655	26.420690185352274	24.454685597064145
20-21	22.916110342372086	26.05013796842506	26.95644081923304	24.077310869969818
22-23	24.116752172716826	25.967000598812085	25.901859554289597	24.01438767418149
24-25	21.962686778528106	27.780182965069937	26.014056184276008	24.243074072125946
26-27	22.47770638786839	27.50530029616922	26.50431306543236	23.51268025053003
28-29	22.187200330167876	27.310871667469154	27.46219861054352	23.039729391819446
30-31	22.47851559339041	26.902846785026462	26.975675282008126	23.642962339575003
32-33	21.74731041402833	27.883491060338166	26.67575670525213	23.693441820381377
34-35	22.128126175919594	27.73814774203207	26.828162996111626	23.305563085936708
36-37	23.54238495281568	26.691513297398796	25.952993735735447	23.813108014050083
38-39	22.971966852259484	26.79739091026803	27.10248608053865	23.128156156933834
40-41	22.52756367461714	26.569157006736667	27.280046934110175	23.62323238453602
42-43	21.516457283890674	27.036879690882238	27.153001153122535	24.29366187210455
44-45	21.377097469885857	26.307441440785297	27.915012765888576	24.400448323440273
46-47	21.45313739136064	26.385404696780878	27.86548950426465	24.29596840759383
48-49	21.419988104726954	26.677779710872034	27.85516898165943	24.04706320274158
50-51	22.590956172719622	26.23648604907102	27.24720010357998	23.925357674629378
52-53	20.855454839753964	26.644545160246036	27.75412754936873	24.745872450631275
54-55	20.3884516559775	26.787383413923564	27.82001739939709	25.004147530701843
56-57	20.58950878314672	26.769072640528325	27.60590965559382	25.035508920731143
58-59	21.02232491512324	26.708966789817218	26.970779733169316	25.29792856189023
60-61	20.47227842978551	25.890327802509105	27.7838931606637	25.853500607041685
62-63	20.65205075912078	25.89710595319025	27.64478327020815	25.80606001748082
64-65	21.106839336301093	26.07122622420073	27.10967219749089	25.712262242007284
66-67	20.83567714129621	26.628356366726695	26.48388336941786	26.05208312255924
68-69	20.779993202673616	27.434899415753613	26.096473482335043	25.68863389923773
70-71	21.393775591124633	26.92388612859894	25.787356972923988	25.894981307352445
72-73	21.81657070488414	26.25927079835078	25.756331252250686	26.167827244514395
74-75	21.52022174115362	26.532057377546685	25.753535517025107	26.194185364274585
76	21.643077371270422	25.80418052487194	25.940132553227645	26.61260955062999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	6.0
16	16.5
17	23.0
18	24.5
19	38.0
20	59.5
21	69.0
22	91.0
23	169.5
24	300.0
25	374.0
26	416.0
27	490.5
28	642.0
29	761.0
30	932.5
31	1256.5
32	1791.0
33	2173.0
34	2404.5
35	2927.0
36	3585.0
37	3952.0
38	4400.0
39	5375.0
40	6438.5
41	7086.5
42	7198.0
43	7324.0
44	7758.5
45	7828.0
46	7589.0
47	7542.0
48	7366.5
49	6927.0
50	6616.0
51	6206.0
52	5279.5
53	4436.0
54	4109.0
55	3780.0
56	3199.5
57	2640.5
58	2333.0
59	2075.5
60	1721.5
61	1474.5
62	1324.0
63	1240.0
64	1080.5
65	978.0
66	848.0
67	745.0
68	707.5
69	646.0
70	591.0
71	560.0
72	508.0
73	470.5
74	459.5
75	434.0
76	391.0
77	315.5
78	270.0
79	257.0
80	227.5
81	187.0
82	151.0
83	126.0
84	120.0
85	94.5
86	72.0
87	69.0
88	56.5
89	41.5
90	33.5
91	26.5
92	25.0
93	21.0
94	13.5
95	7.0
96	4.0
97	3.5
98	4.5
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.004045994869678505
8	0.0032367958957428044
9	0.008901188713292712
10-11	0.0028321964087749535
12-13	0.0
14-15	4.0459948696785054E-4
16-17	0.0
18-19	0.0028321964087749535
20-21	0.0
22-23	8.091989739357011E-4
24-25	0.0028321964087749535
26-27	8.091989739357011E-4
28-29	0.004450594356646356
30-31	8.091989739357011E-4
32-33	4.0459948696785054E-4
34-35	0.004450594356646356
36-37	0.01699317845264972
38-39	0.008901188713292712
40-41	0.0012137984609035515
42-43	0.0012137984609035515
44-45	0.006068992304517758
46-47	0.004045994869678505
48-49	4.0459948696785054E-4
50-51	0.002427596921807103
52-53	0.01537478050477832
54-55	0.009305788200260562
56-57	0.01497018101781047
58-59	0.014160982043874767
60-61	0.02346677024413533
62-63	0.012137984609035516
64-65	0.02346677024413533
66-67	0.021443772809296078
68-69	8.091989739357011E-4
70-71	8.091989739357011E-4
72-73	0.004450594356646356
74-75	0.009305788200260562
76	0.004855193843614206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	123579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.01603023167367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76356378393433	46.22791898299873
2	11.402729334376616	13.914985555798316
3	5.491823932734772	10.052678853203215
4	2.7585109345781995	6.732535463145033
5	1.456175483734069	4.442502366906999
6	0.8302056947336313	3.0393513461024932
7	0.5384401151147833	2.2997434839252624
8	0.3819476678646738	1.8643944359478555
9	0.2891131652586767	1.5876483868618456
>10	1.0861636804901662	9.79373518154379
>50	0.001326207180085673	0.044505943566463556
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0016183979478714022	0.0
2	0.0	0.0	0.0	0.0016183979478714022	0.0
3	0.0	0.0	0.0	0.0016183979478714022	0.0
4	0.0	0.0	0.0	0.004045994869678505	0.0
5	0.0	0.0	0.0	0.004045994869678505	0.0
6	0.0	0.0	0.0	0.004045994869678505	0.0
7	0.0	0.0	0.0	0.004045994869678505	0.0
8	0.0	0.0	0.0	0.004045994869678505	0.0
9	0.0	0.0	0.0	0.004045994869678505	0.0
10	0.0	0.0	0.0	0.005664392817549907	0.0
11	0.0	0.0	0.0	0.005664392817549907	0.0
12	0.0	0.0	0.0	0.005664392817549907	0.0
13	0.0	0.0	0.0	0.005664392817549907	0.0
14	0.0	0.0	0.0	0.005664392817549907	0.0
15	0.0	0.0	0.0	0.005664392817549907	0.0
16	0.0	0.0	0.0	0.005664392817549907	0.0
17	0.0	0.0	0.0	0.005664392817549907	0.0
18	0.0	0.0	0.0	0.005664392817549907	0.0
19	0.0	0.0	0.0	0.00728279076542131	0.0
20	0.0	0.0	0.0	0.00728279076542131	0.0
21	0.0	0.0	0.0	0.00809198973935701	0.0
22	0.0	0.0	0.0	0.012137984609035516	0.0
23	0.0	0.0	0.0	0.012137984609035516	0.0
24	0.0	0.0	0.0	0.018611576400521124	0.0
25	0.0	0.0	0.0	0.021039173322328228	0.0
26	0.0	0.0	0.0	0.02346677024413533	0.0
27	0.0	0.0	0.0	0.02994036203562094	0.0
28	0.0	0.0	0.0	0.03236795895742804	0.0
29	0.0	0.0	0.0	0.04612434151433496	0.0
30	0.0	0.0	0.0	0.06311751996698468	0.0
31	0.0	0.0	0.0	0.0817290963675058	0.0
32	0.0	0.0	0.0	0.09548547892441273	0.0
33	0.0	0.0	0.0	0.12299824403822655	0.0
34	0.0	0.0	0.0	0.1577937999174617	0.0
35	0.0	0.0	0.0	0.21605612604083219	0.0
36	0.0	0.0	0.0	0.3091140080434378	0.0
37	0.0	0.0	0.0	0.4814733894917421	0.0
38	0.0	0.0	0.0	0.6951019186107672	0.0
39	0.0	0.0	0.0	1.0139263143414334	0.0
40	0.0	0.0	0.0	1.3732106587688846	0.0
41	0.0	0.0	0.0	1.7753825488149282	0.0
42	0.0	0.0	0.0	2.235816764984342	0.0
43	0.0	0.0	0.0	2.704342970893113	0.0
44	0.0	0.0	0.0	3.2214211152380257	0.0
45	0.0	0.0	0.0	3.7805776062275953	0.0
46	0.0	0.0	0.0	4.325977714660258	0.0
47	0.0	0.0	0.0	4.952297720486491	0.0
48	0.0	0.0	0.0	5.540585374537745	0.0
49	0.0	0.0	0.0	6.032578350690652	0.0
50	0.0	0.0	0.0	6.641905178064234	0.0
51	0.0	0.0	0.0	7.210772056741032	0.0
52	0.0	0.0	0.0	7.8395196594890715	0.0
53	0.0	0.0	0.0	8.465030466341368	0.0
54	0.0	0.0	0.0	9.049272125522945	0.0
55	0.0	0.0	0.0	9.612474611382194	0.0
56	0.0	0.0	0.0	10.210472653120675	0.0
57	0.0	0.0	0.0	10.771247542058116	0.0
58	0.0	0.0	0.0	11.377337573535957	0.0
59	0.0	0.0	0.0	11.96562522758721	0.0
60	0.0	0.0	0.0	12.600846422126736	0.0
61	0.0	0.0	0.0	13.21017324950032	0.0
62	0.0	0.0	0.0	13.855914030701008	0.0
63	0.0	0.0	0.0	14.42397171040387	0.0
64	0.0	0.0	0.0	15.055956109047655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTGC	15	0.0022215676	69.989075	8
ATCACGA	20	0.006930599	52.513065	54
CTTACGC	20	0.006930599	52.513065	54
TGGACTG	20	0.0069417125	52.491802	5
CGAGCGG	20	0.0069417125	52.491802	25
GATCTCG	20	0.0069417125	52.491802	41
CGGTAGG	20	0.0069417125	52.491802	4
CGCCACG	20	0.0069417125	52.491802	70
CGACGAT	20	0.0069528394	52.47056	22
CGTTAGT	20	0.0069528394	52.47056	16
CTACCCG	20	0.0069528394	52.47056	10
GGATTTG	20	0.0069528394	52.47056	20
GCATGTT	20	0.0069528394	52.47056	11
GTAGATC	50	6.129267E-8	49.01219	38
CGACGAA	50	6.168557E-8	48.97252	22
TTATCCA	35	0.0012474012	40.026165	37
AGATCTC	35	0.0012523836	39.993755	40
GACGGCG	35	0.0012548806	39.97757	14
CTCGAAT	45	9.140852E-5	38.898567	67
GGCAAGC	45	9.184596E-5	38.86708	21
>>END_MODULE